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Protein

Palmitoyltransferase Hip14

Gene

Hip14

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable palmitoyltransferase which is required for photoreceptor synaptic transmission and for the correct expression and localization of palmitoylated protein Csp and synaptosomal-associated protein Snap25 (PubMed:18158335, PubMed:18032660). Probably palmitoylates Csp (PubMed:18158335). Probably also palmitoylates the dorsal-ventral patterning protein Sog and promotes its secretion and activity and the stabilization of the membrane-bound form (PubMed:20599894). Required for synaptic vesicle exocytosis (PubMed:18158335).3 Publications

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei460S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

  • protein-cysteine S-palmitoyltransferase activity Source: FlyBase

GO - Biological processi

  • chemical synaptic transmission Source: FlyBase
  • positive regulation of protein secretion Source: FlyBase
  • protein palmitoylation Source: FlyBase
  • spermatogenesis Source: FlyBase
  • synaptic vesicle exocytosis Source: FlyBase

Keywordsi

Molecular functionAcyltransferase, Transferase

Protein family/group databases

TCDBi9.B.37.1.2. the huntington-interacting protein 14 (hip14) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase Hip141 Publication (EC:2.3.1.225UniRule annotation)
Alternative name(s):
Huntingtin-interacting protein 14 homologCurated
Gene namesi
Name:Hip14Imported
ORF Names:CG6017Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0259824. Hip14.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 295CytoplasmicCuratedAdd BLAST295
Transmembranei296 – 315HelicalSequence analysisAdd BLAST20
Topological domaini316 – 318LumenalCurated3
Transmembranei319 – 341HelicalSequence analysisAdd BLAST23
Topological domaini342 – 345CytoplasmicCurated4
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Topological domaini367 – 373LumenalCurated7
Transmembranei374 – 394HelicalSequence analysisAdd BLAST21
Topological domaini395 – 472CytoplasmicCuratedAdd BLAST78
Transmembranei473 – 493HelicalSequence analysisAdd BLAST21
Topological domaini494 – 520LumenalCuratedAdd BLAST27
Transmembranei521 – 541HelicalSequence analysisAdd BLAST21
Topological domaini542 – 637CytoplasmicCuratedAdd BLAST96

Keywords - Cellular componenti

Cell junction, Cell membrane, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes reduced basal secretion of Sog.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004363081 – 637Palmitoyltransferase Hip14CuratedAdd BLAST637

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ9VUW9.
PRIDEiQ9VUW9.

Expressioni

Tissue specificityi

In stage 13-15 embryos, expressed in the central nervous system. At the third instar larval stage, expressed in the ventral nerve cord and is enriched in the neuropil.1 Publication

Gene expression databases

BgeeiFBgn0259824.
ExpressionAtlasiQ9VUW9. differential.

Interactioni

Subunit structurei

Interacts with dorsal-ventral patterning protein Sog.1 Publication

Protein-protein interaction databases

IntActiQ9VUW9. 2 interactors.
MINTiMINT-299680.

Structurei

3D structure databases

ProteinModelPortaliQ9VUW9.
SMRiQ9VUW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati77 – 106ANK 1Sequence analysisAdd BLAST30
Repeati111 – 140ANK 2Sequence analysisAdd BLAST30
Repeati144 – 173ANK 3Sequence analysisAdd BLAST30
Repeati177 – 207ANK 4Sequence analysisAdd BLAST31
Repeati212 – 242ANK 5Sequence analysisAdd BLAST31
Domaini430 – 480DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.UniRule annotation

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0509. Eukaryota.
COG0666. LUCA.
COG5273. LUCA.
GeneTreeiENSGT00530000063074.
KOiK20032.
OrthoDBiEOG091G056H.
PhylomeDBiQ9VUW9.

Family and domain databases

CDDicd00204. ANK. 2 hits.
Gene3Di1.25.40.20. 2 hits.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR036770. Ankyrin_rpt-contain_sf.
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF12796. Ank_2. 2 hits.
PF01529. DHHC. 1 hit.
SMARTiView protein in SMART
SM00248. ANK. 5 hits.
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS50216. DHHC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VUW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQSACQAAT TGSCVPGTGQ QPDNERQSAL IAQQPPTAPV EPDYSGFDIV
60 70 80 90 100
KATQYGAIAR VRELVESGWD VNQPDSETVT LLHWAAINNR RDIIRYFLEK
110 120 130 140 150
GATVDAVGGE LNATPLHWAT RQGHLGAVVL LMAAGADPRI RDAEGCSCIH
160 170 180 190 200
IAAQFAHTAL VAYFIAKGVD PDLQDRGGMT ALMWAAWKVC ALDPVRLLLT
210 220 230 240 250
LGANPAMVDY THGNTALHWA ILARNATAIS TLVLKSKASL DVPNLRGETP
260 270 280 290 300
LSMLESQTGA IWIGAKVMDR VKEAALTSQQ RRSLLSKLRH DKRLRWWSMV
310 320 330 340 350
ACPFTAFYLA GIVFTVNTLY IIKFFLLGCL YSIFHTIGKA LFDEHLMALL
360 370 380 390 400
PLSVYLATKA WFYVTWLMYI DDAVSFTATV CFLISSLLLW VCFLKSWKGD
410 420 430 440 450
PGIIRPTREQ RFKTIIELSE RGGIGFEPAS FCSGCLVRRP IRSKHCSVCD
460 470 480 490 500
RCVARFDHHC PWVGNCIGLK NHSYFMGFLW MLLIMCAWML YGGSKYYVNQ
510 520 530 540 550
CNVRFDDFLG AMRAIGNCDA WVGWVMGNAL LHMSWVILLT ICQTYQVICL
560 570 580 590 600
GMTTNERMNR GRYRHFQAKG GHSPFTRGPI QNLVDFLECS CFGLVQPKRV
610 620 630
DWMNYYDYDA QTHQTIEKEP LLSVDCPDGM AGDHQYV
Length:637
Mass (Da):71,299
Last modified:May 1, 2000 - v1
Checksum:i0985078D5D091710
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49554.1.
AE014296 Genomic DNA. Translation: AGB94604.1.
AY094779 mRNA. Translation: AAM11132.1.
RefSeqiNP_001261911.1. NM_001274982.1.
NP_648824.1. NM_140567.3.
UniGeneiDm.11460.

Genome annotation databases

EnsemblMetazoaiFBtr0075518; FBpp0075273; FBgn0259824.
FBtr0333577; FBpp0305754; FBgn0259824.
GeneIDi39747.
KEGGidme:Dmel_CG6017.
UCSCiCG6017-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiHIP14_DROME
AccessioniPrimary (citable) accession number: Q9VUW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 8, 2016
Last sequence update: May 1, 2000
Last modified: October 25, 2017
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families