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Protein

Putative U5 small nuclear ribonucleoprotein 200 kDa helicase

Gene

l(3)72Ab

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Putative RNA helicase involved in the second step of RNA splicing.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi503 – 5108ATPPROSITE-ProRule annotation
Nucleotide bindingi1350 – 13578ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: FlyBase
  • nucleic acid binding Source: InterPro
  • RNA helicase activity Source: FlyBase

GO - Biological processi

  • mitotic nuclear division Source: FlyBase
  • mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • RNA splicing Source: FlyBase
  • ventral cord development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-72163. mRNA Splicing - Major Pathway.
R-DME-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative U5 small nuclear ribonucleoprotein 200 kDa helicase (EC:3.6.4.13)
Alternative name(s):
Protein lethal (3) 72Ab
Gene namesi
Name:l(3)72Ab
ORF Names:CG5931
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0263599. l(3)72Ab.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: FlyBase
  • nucleus Source: FlyBase
  • precatalytic spliceosome Source: FlyBase
  • small nuclear ribonucleoprotein complex Source: FlyBase
  • spliceosomal complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21422142Putative U5 small nuclear ribonucleoprotein 200 kDa helicasePRO_0000102089Add
BLAST

Proteomic databases

PaxDbiQ9VUV9.
PRIDEiQ9VUV9.

Expressioni

Gene expression databases

BgeeiQ9VUV9.
GenevisibleiQ9VUV9. DM.

Interactioni

Protein-protein interaction databases

BioGridi65049. 11 interactions.
IntActiQ9VUV9. 7 interactions.
STRINGi7227.FBpp0075282.

Structurei

3D structure databases

ProteinModelPortaliQ9VUV9.
SMRiQ9VUV9. Positions 263-368, 460-896, 985-1285, 1307-1831, 1839-2130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini490 – 673184Helicase ATP-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini684 – 917234Helicase C-terminal 1PROSITE-ProRule annotationAdd
BLAST
Domaini981 – 1286306SEC63 1Add
BLAST
Domaini1337 – 1511175Helicase ATP-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini1544 – 1752209Helicase C-terminal 2PROSITE-ProRule annotationAdd
BLAST
Domaini1811 – 2128318SEC63 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi615 – 6184DEIH box
Motifi1453 – 14564DELQ box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi396 – 3994Poly-Ala

Domaini

Composed of two similar domains.

Sequence similaritiesi

Belongs to the helicase family. SKI2 subfamily.Curated
Contains 2 helicase ATP-binding domains.PROSITE-ProRule annotation
Contains 2 helicase C-terminal domains.PROSITE-ProRule annotation
Contains 2 SEC63 domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0952. Eukaryota.
COG1204. LUCA.
GeneTreeiENSGT00640000091272.
InParanoidiQ9VUV9.
KOiK12854.
OMAiKKMENWW.
OrthoDBiEOG7PGDPQ.
PhylomeDBiQ9VUV9.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.40.50.300. 4 hits.
InterProiIPR000008. C2_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 2 hits.
PF00271. Helicase_C. 1 hit.
PF02889. Sec63. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF52540. SSF52540. 4 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VUV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADAAARQLQ YEYKANSNLV LQADVRLIER PRRDEATGEV CSLVGKLDGT
60 70 80 90 100
RMGDRYQRTK PEKTEERKVK RQKRDEAQYD FERMKGATLL SEGIDEMVGI
110 120 130 140 150
VYRPKTQETR QTYEVLLSFI QEALGDQPRD ILCGAADEIL AVLKNDRLKD
160 170 180 190 200
RERKKDIDSL LGAVTDERFA LLVNLGKKIT DFGSDAVNAL TAAPNNEEQI
210 220 230 240 250
DETYGINVQF EESEEESDND MYGEIRDDDA QDEGEEARID HTLHAENLAN
260 270 280 290 300
EEAANNVKKE RSLHPLDIDA YWLQRCLSKF YKDAMVSQSK AADVLKILKD
310 320 330 340 350
AADDRDCENQ LVLLLGYDCF DFIKQLKLNR QMVLYCTMLA SAQTDSERQR
360 370 380 390 400
IREKMRGNSA LAKILRQLDT GKSEDQEEGE ARGSKRGKGD AEDGGAAAAG
410 420 430 440 450
QVAGVRQLLE LEEMAFTQGS HFMANKRCQL PDGSYRKQRK GYEEVHVPAL
460 470 480 490 500
KPVPFDANEE LQPVDKLPKY VQPVFEGFKT LNRIQSRLYK AALDSDENML
510 520 530 540 550
LCAPTGAGKT NVALLTMMRE IGKHINEDGT INAQDFKIIY VAPMKSLVQE
560 570 580 590 600
MVGNFGRRLA CYNLTVSELT GDHQLTREQI AATQVIVCTP EKWDIITRKG
610 620 630 640 650
GERTFVSLVR LVIIDEIHLL HDERGPVLEA LVARTIRNIE TTQEEVRLVG
660 670 680 690 700
LSATLPNYQD VATFLRVKPD KGLFYFDNSY RPVSLEQQYI GVTEKKALKR
710 720 730 740 750
FQVMNEIVYE KTMEHAGRNQ VLVFVHSRKE TGKTARAVRD MCLEQDTLGS
760 770 780 790 800
FLKEGSASME VLRTEAEQVK NTELKELLPY GFAIHHAGMT RVDRTLVEDL
810 820 830 840 850
FADRHIQVLV STATLAWGVN LPAHTVIIKG TQVYNPEKGR WVELSALDVL
860 870 880 890 900
QMLGRAGRPQ YDTKGEGILI TNHSELQFYL SLLNQQLPIE SQFISKLPDM
910 920 930 940 950
LNAEIVLGTV QHLQDAVNWL GYTYLYIRML RNPTLYGVSH DAIKADPLLE
960 970 980 990 1000
QHRADLLHTA ACCLERSGLI KYDRKTGHFQ VTDLGRIASH YYLTHETMLT
1010 1020 1030 1040 1050
YNQLLKQTLS EIELFRVFSL SSEFRHISVR EEEKLELQKL MERVPIPIKE
1060 1070 1080 1090 1100
SIEEHSAKVN VLLQAYISQL KLEGFALMSD MVFITQSAAR LMRAIFEIVL
1110 1120 1130 1140 1150
TRGWAQLADK TLTLCKMIDR RMWQSMTPLR QFKKMPDEIA KKLEKKHFPW
1160 1170 1180 1190 1200
GRLYDLEPHE LGELIRVPKL GKTIHKFVHQ FPKLELSTHI QPITRGTLRV
1210 1220 1230 1240 1250
ELTITPDFQW DEKVHGQSEG FWVLIEDVDS ELILHHEFFL LKQKYSQDEH
1260 1270 1280 1290 1300
QLKFFVPVFE PLPPQYFLRI VSDRWIGAET QLPVSFRHLI LPEKNMPPTE
1310 1320 1330 1340 1350
LLDLQPLPIS ALRQPKFESF YSQRFPQFNP IQTQVFNAVY NSDENVFVGA
1360 1370 1380 1390 1400
PTGSGKMTIA EFAIMRLFTT QSDARCVYLV SEEALADLVF ADWHSKFGSL
1410 1420 1430 1440 1450
DIKVVKLTGE TGTDLKLIAK GQLVITTADK WDVLSRRWKQ RKNVQLVNLF
1460 1470 1480 1490 1500
IVDELQLVGG EEGPVLEIVC SRMRYISSQI EKQIRIVALS ASLTDARDVA
1510 1520 1530 1540 1550
QWLGCNPNAT FNFHPSVRPI PLELHIQGYN VTHNATRIAT MSKPVYNAIL
1560 1570 1580 1590 1600
KYSAHKPVIV FVSSRKQARL TAIDVLTYAA SDLQPNRFFH AEEEDIKPFL
1610 1620 1630 1640 1650
ERMTDKTLKE TLAQGVAYLH EGLSASDHRL VEQLFDSGAV QVAVISRDLC
1660 1670 1680 1690 1700
WGMSISAHLV IIMDTQFYNG KNHSYEDYPI TDVLQMIGRA NRPNEDADAK
1710 1720 1730 1740 1750
CVLMCQSSKK DFFKKFINEP LPIESHLDHR MHDHFNAEVV TKTIENKQDA
1760 1770 1780 1790 1800
VDYLTWTFLY RRLTQNPNYY NLQGVTHRHL SDHLSELVEN TLSDLEQSKC
1810 1820 1830 1840 1850
ISVEDDMDTL PLNLGMIAAY YYINYTTIEL FSLSLNSKTK VRGLLEIISS
1860 1870 1880 1890 1900
AAEYEDVVVR HHEEQVLRTL SQRLPNKLTG PNETAPKFND PHIKTNLLLQ
1910 1920 1930 1940 1950
AHLSRLQLGP ELQGDTEQIL SKAIRLIQAC VDVLSSNGWL SPAVAAMELA
1960 1970 1980 1990 2000
QMVTQAMWSK DSYLKQLPHF SPEIVKRCTE KKIETVFDIM ELEDEDRTRL
2010 2020 2030 2040 2050
LQLSDLQMAD VARFCNRYPN IELNYEVVDK DRINSGSTVN VVVQLEREDE
2060 2070 2080 2090 2100
VTGPVIAPFF PQKREEGWWV VIGDPKTNSL LSIKRLTLQQ KAKVKLDFVA
2110 2120 2130 2140
PSPGKHDYTL YYMSDSYLGC DQEYKFSIEV GDFQSESESE SD
Length:2,142
Mass (Da):244,510
Last modified:March 1, 2003 - v4
Checksum:i10CFE9E84438F2BE
GO

Sequence cautioni

The sequence AAL39415.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49564.4.
BT016121 mRNA. Translation: AAV37006.1.
AY069270 mRNA. Translation: AAL39415.1. Different initiation.
RefSeqiNP_648818.3. NM_140561.4.
UniGeneiDm.973.

Genome annotation databases

EnsemblMetazoaiFBtr0075527; FBpp0075282; FBgn0263599.
GeneIDi39737.
KEGGidme:Dmel_CG5931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49564.4.
BT016121 mRNA. Translation: AAV37006.1.
AY069270 mRNA. Translation: AAL39415.1. Different initiation.
RefSeqiNP_648818.3. NM_140561.4.
UniGeneiDm.973.

3D structure databases

ProteinModelPortaliQ9VUV9.
SMRiQ9VUV9. Positions 263-368, 460-896, 985-1285, 1307-1831, 1839-2130.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65049. 11 interactions.
IntActiQ9VUV9. 7 interactions.
STRINGi7227.FBpp0075282.

Proteomic databases

PaxDbiQ9VUV9.
PRIDEiQ9VUV9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075527; FBpp0075282; FBgn0263599.
GeneIDi39737.
KEGGidme:Dmel_CG5931.

Organism-specific databases

FlyBaseiFBgn0263599. l(3)72Ab.

Phylogenomic databases

eggNOGiKOG0952. Eukaryota.
COG1204. LUCA.
GeneTreeiENSGT00640000091272.
InParanoidiQ9VUV9.
KOiK12854.
OMAiKKMENWW.
OrthoDBiEOG7PGDPQ.
PhylomeDBiQ9VUV9.

Enzyme and pathway databases

ReactomeiR-DME-72163. mRNA Splicing - Major Pathway.
R-DME-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

GenomeRNAii39737.
PROiQ9VUV9.

Gene expression databases

BgeeiQ9VUV9.
GenevisibleiQ9VUV9. DM.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.40.50.300. 4 hits.
InterProiIPR000008. C2_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 2 hits.
PF00271. Helicase_C. 1 hit.
PF02889. Sec63. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF52540. SSF52540. 4 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1212-2130.
    Strain: Berkeley.
    Tissue: Ovary.

Entry informationi

Entry nameiU520_DROME
AccessioniPrimary (citable) accession number: Q9VUV9
Secondary accession number(s): Q5U0X1, Q8T9I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 123 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.