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Protein

Actin-interacting protein 1

Gene

flr

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Essential for organismal and cell viability. Required for the development of normal wing cell planar polarity.1 Publication

GO - Molecular functioni

  • actin binding Source: FlyBase

GO - Biological processi

  • actin filament depolymerization Source: FlyBase
  • border follicle cell migration Source: FlyBase
  • establishment of planar polarity Source: UniProtKB
  • imaginal disc-derived wing hair organization Source: FlyBase
  • negative regulation of Arp2/3 complex-mediated actin nucleation Source: FlyBase
  • phagocytosis Source: FlyBase
  • regulation of actin filament depolymerization Source: UniProtKB
  • regulation of actin filament polymerization Source: FlyBase
  • sarcomere organization Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-DME-114608. Platelet degranulation.
SignaLinkiQ9VU68.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-interacting protein 1
Short name:
AIP1
Alternative name(s):
Protein flare
Gene namesi
Name:flr
ORF Names:CG10724
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0260049. flr.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication

  • Note: At the cell periphery of wing cells.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Pupals exhibit grossly abnormal epidermal hairs on wings, an abnormal accumulation of F-actin and microtubules, and disruption of the frizzled-based planar cell polarity system.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Actin-interacting protein 1PRO_0000051346Add
BLAST

Proteomic databases

PaxDbiQ9VU68.
PRIDEiQ9VU68.

Expressioni

Tissue specificityi

Expressed in pupal wing cells.1 Publication

Gene expression databases

BgeeiQ9VU68.
ExpressionAtlasiQ9VU68. differential.
GenevisibleiQ9VU68. DM.

Interactioni

GO - Molecular functioni

  • actin binding Source: FlyBase

Protein-protein interaction databases

BioGridi64849. 8 interactions.
DIPiDIP-18795N.
IntActiQ9VU68. 2 interactions.
MINTiMINT-318098.
STRINGi7227.FBpp0075581.

Structurei

3D structure databases

ProteinModelPortaliQ9VU68.
SMRiQ9VU68. Positions 9-605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati62 – 10140WD 1Add
BLAST
Repeati106 – 14944WD 2Add
BLAST
Repeati150 – 19041WD 3Add
BLAST
Repeati193 – 23240WD 4Add
BLAST
Repeati237 – 27640WD 5Add
BLAST
Repeati323 – 36240WD 6Add
BLAST
Repeati366 – 40338WD 7Add
BLAST
Repeati444 – 48340WD 8Add
BLAST
Repeati487 – 52640WD 9Add
BLAST
Repeati531 – 57040WD 10Add
BLAST
Repeati575 – 60733WD 11Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat AIP1 family.Curated
Contains 11 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0318. Eukaryota.
ENOG410XQN1. LUCA.
GeneTreeiENSGT00390000009416.
InParanoidiQ9VU68.
OMAiWDVGANS.
OrthoDBiEOG7TXKG1.
PhylomeDBiQ9VU68.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 11 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q9VU68-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQPQPPAYE NKNIYATLPR TQRGQPIVLG ADPKGKNFLY TNGNSVIIRN
60 70 80 90 100
IENPAIADVY TEHSCAVNVA KYSPSGFYIA SGDASGKIRI WDTVNKEHLL
110 120 130 140 150
KNEFQPIAGP IKDISWSPDN QRIVAVGEGR ERFGHVFMSE TGTSVGEISG
160 170 180 190 200
QSKSINSADF RPARPFRIVT GSEDNTIAVF EGPPFKFKMT KQDHSRFVQA
210 220 230 240 250
VRYSPDGKFF ASAGFDGKVF LYDGTSSELV GEFGSPAHKG GVYALAWKPD
260 270 280 290 300
STQLLTCSGD KTCRLWTVES RELVSEFVMG TTVDDQQVSC LWQGDNLITV
310 320 330 340 350
SLSGVITYLN VADPSKPLRV VKGHNKPITV LGLSDDRSTI YTGSHDGVVT
360 370 380 390 400
NWNSGSGTND RITGTGHGNQ INGIAAWGDF VYTCGIDDSL RQFSVEGNSY
410 420 430 440 450
TDYVVKLNCQ PRGLAILRNE NIIALACIKE LTLVQDQKKI FSLPIKYEAS
460 470 480 490 500
SIAVNADTSD VAVGGDDQKL HIYTLKGGVL EPKVELDHLG AVTDVSYSPD
510 520 530 540 550
LKYLVACDAH RKVVLYSVEE YKPAHNKEWG FHSARVNTVA WSPNSLLVAS
560 570 580 590 600
GSLDTTIIIW SVANPAKHTI IKNAHPQSQI TRLVWLDNNT VISTGQDCNT

KVWHVENI
Length:608
Mass (Da):66,550
Last modified:May 1, 2000 - v1
Checksum:iA43F0BC8EA0AE123
GO
Isoform B (identifier: Q9VU68-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MAQPQPPAYENK → MSQQAKNE

Show »
Length:604
Mass (Da):66,111
Checksum:i1E6F8DE270D3BBC7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1212MAQPQ…AYENK → MSQQAKNE in isoform B. CuratedVSP_036571Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49822.1.
AE014296 Genomic DNA. Translation: AAN11835.1.
AY061340 mRNA. Translation: AAL28888.1.
RefSeqiNP_648642.1. NM_140385.4. [Q9VU68-1]
NP_729891.1. NM_168543.2. [Q9VU68-2]
UniGeneiDm.1492.

Genome annotation databases

EnsemblMetazoaiFBtr0075845; FBpp0075581; FBgn0260049. [Q9VU68-1]
GeneIDi39505.
KEGGidme:Dmel_CG10724.
UCSCiCG10724-RA. d. melanogaster. [Q9VU68-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49822.1.
AE014296 Genomic DNA. Translation: AAN11835.1.
AY061340 mRNA. Translation: AAL28888.1.
RefSeqiNP_648642.1. NM_140385.4. [Q9VU68-1]
NP_729891.1. NM_168543.2. [Q9VU68-2]
UniGeneiDm.1492.

3D structure databases

ProteinModelPortaliQ9VU68.
SMRiQ9VU68. Positions 9-605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64849. 8 interactions.
DIPiDIP-18795N.
IntActiQ9VU68. 2 interactions.
MINTiMINT-318098.
STRINGi7227.FBpp0075581.

Proteomic databases

PaxDbiQ9VU68.
PRIDEiQ9VU68.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075845; FBpp0075581; FBgn0260049. [Q9VU68-1]
GeneIDi39505.
KEGGidme:Dmel_CG10724.
UCSCiCG10724-RA. d. melanogaster. [Q9VU68-1]

Organism-specific databases

CTDi797829.
FlyBaseiFBgn0260049. flr.

Phylogenomic databases

eggNOGiKOG0318. Eukaryota.
ENOG410XQN1. LUCA.
GeneTreeiENSGT00390000009416.
InParanoidiQ9VU68.
OMAiWDVGANS.
OrthoDBiEOG7TXKG1.
PhylomeDBiQ9VU68.

Enzyme and pathway databases

ReactomeiR-DME-114608. Platelet degranulation.
SignaLinkiQ9VU68.

Miscellaneous databases

GenomeRNAii39505.
PROiQ9VU68.

Gene expression databases

BgeeiQ9VU68.
ExpressionAtlasiQ9VU68. differential.
GenevisibleiQ9VU68. DM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 11 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The flare gene, which encodes the AIP1 protein of Drosophila, functions to regulate F-actin disassembly in pupal epidermal cells."
    Ren N., Charlton J., Adler P.N.
    Genetics 176:2223-2234(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiWDR1_DROME
AccessioniPrimary (citable) accession number: Q9VU68
Secondary accession number(s): Q8IQJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.