Q9VU27 (OR69A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative odorant receptor 69a, isoform A

Gene names

Name:	Or69a
Synonyms:	Or69b
ORF Names:	CG32116

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Complexes with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide By similarity.
Subunit structure	Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Orco complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the heteromeric odorant receptor channel (TC 1.A.69) family. [View classification]

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VU27-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B (identifier: P82985-1)

The sequence of this isoform can be found in the external entry P82985.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Putative odorant receptor 69a, isoform A		PRO_0000174266
Regions
	Topological domain	1 – 39	39	Cytoplasmic Potential
	Transmembrane	40 – 60	21	Helical; Name=1; Potential
	Topological domain	61 – 76	16	Extracellular Potential
	Transmembrane	77 – 97	21	Helical; Name=2; Potential
	Topological domain	98 – 139	42	Cytoplasmic Potential
	Transmembrane	140 – 160	21	Helical; Name=3; Potential
	Topological domain	161 – 189	29	Extracellular Potential
	Transmembrane	190 – 210	21	Helical; Name=4; Potential
	Topological domain	211 – 269	59	Cytoplasmic Potential
	Transmembrane	270 – 290	21	Helical; Name=5; Potential
	Topological domain	291 – 304	14	Extracellular Potential
	Transmembrane	305 – 325	21	Helical; Name=6; Potential
	Topological domain	326 – 365	40	Cytoplasmic Potential
	Transmembrane	366 – 386	21	Helical; Name=7; Potential
	Topological domain	387 – 393	7	Extracellular Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified January 16, 2004. Version 2. Checksum: 4C2922FAEF58E17D	FASTA	393	45,440
	10 20 30 40 50 60 MQLHDHMKYI DLGCKMACIP RYQWKGRPTE RQFYASEQRI VFLLGTICQI FQITGVLIYW 70 80 90 100 110 120 YCNGRLATET GTFVAQLSEM CSSFCLTFVG FCNVYAISTN RNQIETLLEE LHQIYPRYRK 130 140 150 160 170 180 NHYRCQHYFD MAMTIMRIEF LFYMILYVYY NSAPLWVLLW EHLHEEYDLS FKTQTNTWFP 190 200 210 220 230 240 WKVHGSALGF GMAVLSITVG SFVGVGFSIV TQNLICLLTF QLKLHYDGIS SQLVSLDCRR 250 260 270 280 290 300 PGAHKELSIL IAHHSRILQL GDQVNDIMNF VFGSSLVGAT IAICMSSVSI MLLDLASAFK 310 320 330 340 350 360 YASGLVAFVL YNFVICYMGT EVTLASGKVL PAAFYNNWYE GDLVYRRMLL ILMMRATKPY 370 380 390 MWKTYKLAPV SITTYMATLK FSYQMFTCVR SLK
	Isoform B [UniParc]. See P82985.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"Molecular evolution of the insect chemoreceptor gene superfamily in Drosophila melanogaster." Robertson H.M., Warr C.G., Carlson J.R. Proc. Natl. Acad. Sci. U.S.A. 100:14537-14542(2003) [PubMed: 14608037] [Abstract] Cited for: IDENTIFICATION OF ALTERNATIVE SPLICING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014296 Genomic DNA. Translation: AAF49864.3.
RefSeq	NP_996070.1. NM_206348.1.
UniGene	Dm.26498.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VU27. 1 interaction.
STRING	Q9VU27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0075901; FBpp0089412; FBgn0041622.
GeneID	2768964.
KEGG	dme:Dmel_CG33264.
NMPDR	fig|7227.3.peg.9649.
Organism-specific databases
CTD	2768964.
FlyBase	FBgn0041622. Or69a.
Phylogenomic databases
GeneTree	EMGT00050000009320.
InParanoid	Q9VU27.
OMA	LKFSYQL.
OrthoDB	EOG44J10S.
PhylomeDB	Q9VU27.
Gene expression databases
Bgee	Q9VU27.
GermOnline	CG33264. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
KO	K08471.
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	848843.

Entry information

Entry name

OR69A_DROME

Accession

Primary (citable) accession number: Q9VU27

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 16, 2004
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project