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Protein

Ubiquitin-conjugating enzyme E2 C

Gene

vih

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.PROSITE-ProRule annotation2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei116Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: FlyBase
  • exit from mitosis Source: UniProtKB
  • positive regulation of cellular protein catabolic process Source: FlyBase
  • positive regulation of mitotic metaphase/anaphase transition Source: FlyBase
  • protein polyubiquitination Source: GO_Central

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-DME-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-DME-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-DME-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-DME-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-DME-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-DME-176408 Regulation of APC/C activators between G1/S and early anaphase
R-DME-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-DME-176412 Phosphorylation of the APC/C
R-DME-179409 APC-Cdc20 mediated degradation of Nek2A
R-DME-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-DME-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ9VTY6
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 C (EC:2.3.2.23)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme C (EC:2.3.2.24)
E2 ubiquitin-conjugating enzyme C
Ubiquitin carrier protein C
Ubiquitin carrier protein vihar
Ubiquitin-protein ligase C
Gene namesi
Name:vih
Synonyms:Ubch10
ORF Names:CG10682
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0264848 vih

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality. Both homozygous and hemizygous females show maternal effect lethality and produce syncytial embryos that fail to develop as a result of mitotic defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003904711 – 178Ubiquitin-conjugating enzyme E2 CAdd BLAST178

Post-translational modificationi

Ubiquitinated by the APC/C complex, leading to its degradation at the metaphase-anaphase transition.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9VTY6
PRIDEiQ9VTY6

Expressioni

Gene expression databases

BgeeiFBgn0264848
GenevisibleiQ9VTY6 DM

Interactioni

Subunit structurei

Component of the APC/C complex.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi68841, 9 interactors
IntActiQ9VTY6, 6 interactors
STRINGi7227.FBpp0075690

Structurei

3D structure databases

ProteinModelPortaliQ9VTY6
SMRiQ9VTY6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0421 Eukaryota
ENOG4111IGV LUCA
GeneTreeiENSGT00760000119350
InParanoidiQ9VTY6
KOiK06688
OMAiAELWDKD
OrthoDBiEOG091G0M7S
PhylomeDBiQ9VTY6

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VTY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQNISPEQS GGAGGGGSKH SDDSMPVKDN HAVSKRLHKE LMNLMMANER
60 70 80 90 100
GISAFPDGEN IFKWVGTIAG PRNTVYSGQT YRLSLDFPNS YPYAAPVVKF
110 120 130 140 150
LTSCFHPNVD LQGAICLDIL KDKWSALYDV RTILLSIQSL LGEPNNESPL
160 170
NAQAAMMWND QKEYKKYLDA FYEKHKDT
Length:178
Mass (Da):19,832
Last modified:May 1, 2000 - v1
Checksum:i7EF04EE68FEDC7AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF410850 mRNA Translation: AAL02117.1
AE014296 Genomic DNA Translation: AAF49909.1
BT028772 mRNA Translation: ABI34153.1
RefSeqiNP_648582.1, NM_140325.3
UniGeneiDm.3505

Genome annotation databases

EnsemblMetazoaiFBtr0075958; FBpp0075690; FBgn0264848
GeneIDi44118
KEGGidme:Dmel_CG10682
UCSCiCG10682-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiUBE2C_DROME
AccessioniPrimary (citable) accession number: Q9VTY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health