Q9VTI8 (CHRB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein charybde

Alternative name(s):
Protein charybdis
Protein regulated in development and DNA damage response 1
Short name=REDD-1

Gene names

Name:	chrb
Synonyms:	char
ORF Names:	CG7533

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits cell growth by regulating the Tor pathway upstream of the Tsc1-Tsc2 complex and downstream of Akt1. Acts as cell death activator during head development. Ref.6 Ref.7 Ref.8
Subcellular location	Cytoplasm By similarity.
Developmental stage	Expression peaks 2-8 hours after egg laying. Expressed in the dorsal domains of gastrulation stage embryos. During mid-embryonic development, expressed in the central nervous system, the three thoracic segments and the cardiac precursor cells. Ref.8
Induction	Expression is controlled by homeobox transcription factors. At larval stages, induced by starvation, and mildly by hypoxia in midgut but not fat body. Ref.6 Ref.8
Sequence similarities	Belongs to the DDIT4 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from mutant phenotype Ref.8. Source: UniProtKB head involution Inferred from mutant phenotype Ref.8. Source: UniProtKB negative regulation of growth Inferred from genetic interaction Ref.6. Source: FlyBase negative regulation of signal transduction Inferred from electronic annotation. Source: InterPro regulation of TOR signaling cascade Inferred from mutant phenotype Ref.8. Source: UniProtKB response to oxidative stress Inferred from mutant phenotype Ref.6. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Protein charybde		PRO_0000307209
Experimental info
	Sequence conflict	154	1	C → G in AAF59840. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9VTI8 [UniParc]. Last modified March 1, 2001. Version 2. Checksum: CD224D3BFBF315E7	FASTA	299	32,179
	10 20 30 40 50 60 MKMEVLSVQN HIQGKFGVNK IKDWQASTAP LEEEEELTAG VNGNTAAGEG ILDVDVVDGH 70 80 90 100 110 120 PASVLHMRQH QALNTRPSAT PPSAGGGGPL AGGGSVGMTT PKQATSPVAA ASFEAPLSGG 130 140 150 160 170 180 SAAAYHHAYM TNVLSSTAQQ HHPLPASPLQ STACARFGAA DNLDDVSASA VRELSQQLQA 190 200 210 220 230 240 QLRDAKRRHL ACTEVTLPND LTQRIAAEII RMSEREPCGE RACTLFIEFE SEPNKVKRIA 250 260 270 280 290 YFKVDPDTVS IFELYLTLRQ DKSGWSSLVP QFIKNLTRSN TINISPDFTL TKKKLYSSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of charybde and scylla, two paralogous target genes of Hox and cofactors proteins in Drosophila." Chauvet S., Maurel-Zaffran C., Miassod R., Jullien N., Pradel J., Aragnol D. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"REDD1, a developmentally regulated transcriptional target of p63 and p53, links p63 to regulation of reactive oxygen species." Ellisen L.W., Ramsayer K.D., Johannessen C.M., Yang A., Beppu H., Minda K., Oliner J.D., McKeon F., Haber D.A. Mol. Cell 10:995-1005(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"The hypoxia-induced paralogs Scylla and Charybdis inhibit growth by down-regulating S6K activity upstream of TSC in Drosophila." Reiling J.H., Hafen E. Genes Dev. 18:2879-2892(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[7]	"The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway." Corradetti M.N., Inoki K., Guan K.-L. J. Biol. Chem. 280:9769-9772(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"scylla and charybde, homologues of the human apoptotic gene RTP801, are required for head involution in Drosophila." Scuderi A., Simin K., Kazuko S.G., Metherall J.E., Letsou A. Dev. Biol. 291:110-122(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF221109 mRNA. Translation: AAF59840.1. AE014296 Genomic DNA. Translation: AAF50060.2. AY060367 mRNA. Translation: AAL25406.1.
RefSeq	NP_001261711.1. NM_001274782.1. NP_648470.2. NM_140213.4.
UniGene	Dm.20385.
3D structure databases
ProteinModelPortal	Q9VTI8.
SMR	Q9VTI8. Positions 164-296.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VTI8. 1 interaction.
MINT	MINT-912909.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076093; FBpp0099774; FBgn0036165. FBtr0331841; FBpp0304225; FBgn0036165.
GeneID	39284.
KEGG	dme:Dmel_CG7533.
Organism-specific databases
CTD	39284.
FlyBase	FBgn0036165. chrb.
Phylogenomic databases
eggNOG	NOG71738.
GeneTree	ENSGT00530000063652.
InParanoid	Q9VTI8.
OMA	CGERACT.
OrthoDB	EOG4FJ6RK.
PhylomeDB	Q9VTI8.
Gene expression databases
Bgee	Q9VTI8.
Family and domain databases
InterPro	IPR012918. RTP801-like. [Graphical view]
PANTHER	PTHR12478. PTHR12478. 1 hit.
Pfam	PF07809. RTP801_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	chrb. drosophila.
GenomeRNAi	39284.
NextBio	812898.

Entry information

Entry name

CHRB_DROME

Accession

Primary (citable) accession number: Q9VTI8
Secondary accession number(s): Q9NHN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project