Q9VTH4 (SCLLA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein scylla

Alternative name(s):
Protein regulated in development and DNA damage response 2
Short name=REDD-2

Gene names

Name:	scyl
ORF Names:	CG7590

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits cell growth by regulating the Tor pathway upstream of the Tsc1-Tsc2 complex and downstream of Akt1. Acts as cell death activator during head development. Ref.7 Ref.8 Ref.9
Subcellular location	Cytoplasm By similarity.
Developmental stage	Expression peaks 2-8 hours after egg laying. Expressed in the dorsal domains of gastrulation stage embryos. During mid-embryonic developmental stages, expressed in the central nervous system, the three thoracic segments and the cardiac precursor cells. Ref.9
Induction	Expression is controlled by homeobox transcription factors. At larval stages, induced by starvation, and by hypoxia in midgut and fat body. Ref.7 Ref.9
Sequence similarities	Belongs to the DDIT4 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from mutant phenotype Ref.9. Source: UniProtKB head involution Inferred from mutant phenotype Ref.9. Source: UniProtKB multicellular organism reproduction Inferred from expression pattern PubMed 11404480. Source: FlyBase negative regulation of growth Inferred from genetic interaction Ref.7. Source: FlyBase negative regulation of signal transduction Inferred from electronic annotation. Source: InterPro regulation of TOR signaling cascade Inferred from mutant phenotype Ref.9. Source: UniProtKB response to DNA damage stimulus Inferred from mutant phenotype PubMed 19543366. Source: FlyBase response to oxidative stress Inferred from mutant phenotype Ref.7. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VTH4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9VTH4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Protein scylla		PRO_0000307211
Natural variations
	Alternative sequence	1 – 39	39	Missing in isoform B.		VSP_028636
Experimental info
	Sequence conflict	21	1	K → E in AAV36860. Ref.4
	Sequence conflict	203	1	S → P in AAF59841. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 894841884CD28258	FASTA	280	30,762
	10 20 30 40 50 60 MKMDVIAREQ IIYGSLQGSN KNKDWTSRLP PPSAYTLDLM SKKAKTTTGG SSNGSNATAT 70 80 90 100 110 120 STTTSTSSSI KHKQPAGSSN NNVGQSQSKK TKPSGSYNSN SNYYYYAADE EEGGSADYAL 130 140 150 160 170 180 SNYDKKAVEE LSLRLLDELR AAKSRHLTCT EVSLPCDLTP SVAREIIRVS EKEPRGIRGC 190 200 210 220 230 240 TIYIEFEDEP KNSRRIASIK VDSDTVSTFE VYLTLRQDHR GWTSLLPQFM KSLARTITIS 250 260 270 280 PEYTITKNKL YSADGLGARR SYSFGSHAHR PSAAIATPTN
	Isoform B [UniParc]. Checksum: E0EB30B227B1A7F6	FASTA	241	26,342
	10 20 30 40 50 60 MSKKAKTTTG GSSNGSNATA TSTTTSTSSS IKHKQPAGSS NNNVGQSQSK KTKPSGSYNS 70 80 90 100 110 120 NSNYYYYAAD EEEGGSADYA LSNYDKKAVE ELSLRLLDEL RAAKSRHLTC TEVSLPCDLT 130 140 150 160 170 180 PSVAREIIRV SEKEPRGIRG CTIYIEFEDE PKNSRRIASI KVDSDTVSTF EVYLTLRQDH 190 200 210 220 230 240 RGWTSLLPQF MKSLARTITI SPEYTITKNK LYSADGLGAR RSYSFGSHAH RPSAAIATPT N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of charybde and scylla, two paralogous target genes of Hox and cofactors proteins in Drosophila." Chauvet S., Maurel-Zaffran C., Miassod R., Jullien N., Pradel J., Aragnol D. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING (ISOFORMS A AND B). Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B). Strain: Berkeley. Tissue: Embryo.
[5]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley.
[6]	"REDD1, a developmentally regulated transcriptional target of p63 and p53, links p63 to regulation of reactive oxygen species." Ellisen L.W., Ramsayer K.D., Johannessen C.M., Yang A., Beppu H., Minda K., Oliner J.D., McKeon F., Haber D.A. Mol. Cell 10:995-1005(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[7]	"The hypoxia-induced paralogs Scylla and Charybdis inhibit growth by down-regulating S6K activity upstream of TSC in Drosophila." Reiling J.H., Hafen E. Genes Dev. 18:2879-2892(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[8]	"The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway." Corradetti M.N., Inoki K., Guan K.-L. J. Biol. Chem. 280:9769-9772(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"scylla and charybde, homologues of the human apoptotic gene RTP801, are required for head involution in Drosophila." Scuderi A., Simin K., Kazuko S.G., Metherall J.E., Letsou A. Dev. Biol. 291:110-122(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF221110 mRNA. Translation: AAF59841.1. AE014296 Genomic DNA. Translation: AAF50074.1. AE014296 Genomic DNA. Translation: AAS65038.1. AY075382 mRNA. Translation: AAL68220.1. BT015975 mRNA. Translation: AAV36860.1. BT057991 mRNA. Translation: ACM16701.1.
RefSeq	NP_648456.2. NM_140199.3.
UniGene	Dm.6574.
3D structure databases
ProteinModelPortal	Q9VTH4.
SMR	Q9VTH4. Positions 154-251.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-976501.
STRING	7227.FBpp0075899.
Proteomic databases
PRIDE	Q9VTH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076168; FBpp0075899; FBgn0041094.
GeneID	39270.
KEGG	dme:Dmel_CG7590.
UCSC	CG7590-RA. d. melanogaster.
Organism-specific databases
CTD	39270.
FlyBase	FBgn0041094. scyl.
Phylogenomic databases
eggNOG	NOG71738.
GeneTree	ENSGT00530000063652.
InParanoid	Q9VTH4.
OMA	ISPEYTI.
OrthoDB	EOG4FJ6RK.
PhylomeDB	Q9VTH4.
Gene expression databases
Bgee	Q9VTH4.
Family and domain databases
InterPro	IPR012918. RTP801-like. [Graphical view]
PANTHER	PTHR12478. PTHR12478. 1 hit.
Pfam	PF07809. RTP801_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	scyl. drosophila.
GenomeRNAi	39270.
NextBio	812820.

Entry information

Entry name

SCLLA_DROME

Accession

Primary (citable) accession number: Q9VTH4
Secondary accession number(s): B9EQR6 , Q5U1B7, Q8SY44, Q9NHN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project