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Q9VT57 (CDK8_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 8

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division protein kinase 8
Short name=DmCdk8
Mediator complex subunit Cdk8
Mediator of RNA polymerase II transcription subunit Cdk8
Gene names
Name:Cdk8
ORF Names:CG10572
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length454 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May phosphorylate the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Required for leg and eye development and macrochaete specification or differentiation. Ref.1 Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1 UniProtKB P49336

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactor

Magnesium By similarity. UniProtKB P49336

Subunit structure

Component of the Cdk8 module of the Mediator complex, composed of CycC, Cdk8, kto and skd.

Subcellular location

Nucleus Ref.1.

Developmental stage

Expressed both maternally and zygotically during developmental periods of maximal cell division; most abundant in early embryos and low levels in larvae, pupae and adults. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionActivator
Kinase
Repressor
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG1/S transition of mitotic cell cycle

Inferred from mutant phenotype Ref.1. Source: UniProtKB

axon guidance

Inferred from mutant phenotype PubMed 18516287. Source: FlyBase

chaeta development

Inferred from mutant phenotype Ref.5. Source: FlyBase

imaginal disc-derived leg segmentation

Inferred from mutant phenotype Ref.5. Source: FlyBase

mitotic G2 DNA damage checkpoint

Inferred from genetic interaction PubMed 21205937. Source: FlyBase

mitotic cell cycle

Inferred from mutant phenotype PubMed 14527345. Source: FlyBase

protein phosphorylation

Inferred from direct assay Ref.1. Source: UniProtKB

regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity PubMed 11090137. Source: GOC

sex comb development

Inferred from mutant phenotype Ref.5. Source: FlyBase

snRNA 3'-end processing

Inferred from direct assay PubMed 23097424. Source: FlyBase

transcription initiation from RNA polymerase II promoter

Inferred from sequence or structural similarity PubMed 11090137. Source: FlyBase

   Cellular_componentmediator complex

Inferred from direct assay PubMed 12556495. Source: FlyBase

nucleus

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA polymerase II carboxy-terminal domain kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

RNA polymerase II transcription cofactor activity

Inferred from sequence or structural similarity PubMed 11090137. Source: FlyBase

cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein serine/threonine kinase activity

Non-traceable author statement PubMed 10908587. Source: FlyBase

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CycCP250082EBI-163640,EBI-195485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 454454Cyclin-dependent kinase 8
PRO_0000085798

Regions

Domain21 – 335315Protein kinase
Nucleotide binding27 – 359ATP By similarity UniProtKB P49336
Compositional bias328 – 451124Gln-rich

Sites

Active site1511Proton acceptor By similarity UniProtKB P49336
Binding site521ATP By similarity UniProtKB P49336

Experimental info

Sequence conflict91T → A in AAB38385. Ref.1
Sequence conflict151K → N in AAB38385. Ref.1
Sequence conflict371K → R in AAB38385. Ref.1
Sequence conflict401W → R in AAB38385. Ref.1
Sequence conflict701L → W in AAB38385. Ref.1
Sequence conflict1851K → R in AAM50971. Ref.4
Sequence conflict3211N → I in AAB38385. Ref.1
Sequence conflict3361Q → R in AAB38385. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9VT57 [UniParc].

Last modified March 1, 2001. Version 2.
Checksum: D73F6219E2A03C98

FASTA45453,682
        10         20         30         40         50         60 
MDYDFKMKTQ IERTKVEDLF NYEGCKVGRG TYGHVYKAKW KETSDGKEYA LKQIDGTGLS 

        70         80         90        100        110        120 
MSACREIALL RELKHQNVIT LIRVFLSHND RKVFLLIDYA EHDLWHIIKF HRAAKATKKQ 

       130        140        150        160        170        180 
VVVPRGMVKS LLYQILDGIH YLHSNWVLHR DLKPANILVM GDGNERGRVK IADMGFARLF 

       190        200        210        220        230        240 
NAPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED 

       250        260        270        280        290        300 
IKTSNPYHHD QLDRIFNVMG FPQDKDWEDI KKMPEHHTLT KDFKRSTYST CSLAKYMERH 

       310        320        330        340        350        360 
KIKPDSKAFH LLQKLLLMDP NKRITSEQAM QDQYFQEEPQ PTQDVFAGCP IPYPKREFLT 

       370        380        390        400        410        420 
DDDQEDKSDN KRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ MNAEPNAKRV RLSGAGNQQD 

       430        440        450 
FHHQQQQQQQ QQQQQQQQQQ QMMFNQQQNF QRFN 

« Hide

References

« Hide 'large scale' references
[1]"Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II."
Leclerc V., Tassan J.-P., O'Farrell P.H., Nigg E.A., Leopold P.
Mol. Biol. Cell 7:505-513(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[5]"Distinct roles for Mediator Cdk8 module subunits in Drosophila development."
Loncle N., Boube M., Joulia L., Boschiero C., Werner M., Cribbs D.L., Bourbon H.-M.
EMBO J. 26:1045-1054(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CYCC; KTO AND SKD.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33015 mRNA. Translation: AAB38385.1.
AE014296 Genomic DNA. Translation: AAF50197.2.
AY119111 mRNA. Translation: AAM50971.1.
RefSeqNP_536735.2. NM_080487.3.
UniGeneDm.5731.

3D structure databases

ProteinModelPortalQ9VT57.
SMRQ9VT57. Positions 1-359.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid64544. 32 interactions.
DIPDIP-38574N.
IntActQ9VT57. 3 interactions.
MINTMINT-4329200.
STRING7227.FBpp0076098.

Proteomic databases

PaxDbQ9VT57.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0076369; FBpp0076098; FBgn0015618.
GeneID39157.
KEGGdme:Dmel_CG10572.

Organism-specific databases

CTD1024.
FlyBaseFBgn0015618. Cdk8.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00530000064012.
InParanoidQ9VT57.
KOK02208.
OMANVITLIR.
OrthoDBEOG76739K.
PhylomeDBQ9VT57.

Enzyme and pathway databases

SignaLinkQ9VT57.

Gene expression databases

BgeeQ9VT57.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi39157.
NextBio812240.
PROQ9VT57.

Entry information

Entry nameCDK8_DROME
AccessionPrimary (citable) accession number: Q9VT57
Secondary accession number(s): P91642, Q8MS41
Entry history
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase