Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cyclin-dependent kinase 8

Gene

Cdk8

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May phosphorylate the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Required for leg and eye development and macrochaete specification or differentiation.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 PublicationBy similarity
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52ATPPROSITE-ProRule annotationBy similarity1
Active sitei151Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

GO - Biological processi

  • axon guidance Source: FlyBase
  • chaeta development Source: FlyBase
  • G1/S transition of mitotic cell cycle Source: UniProtKB
  • imaginal disc-derived leg segmentation Source: FlyBase
  • protein phosphorylation Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • sex comb development Source: FlyBase
  • snRNA 3'-end processing Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-212436 Generic Transcription Pathway
R-DME-2173796 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
SignaLinkiQ9VT57

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 8 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 8
Short name:
DmCdk8
Mediator complex subunit Cdk8
Mediator of RNA polymerase II transcription subunit Cdk8
Gene namesi
Name:Cdk8
ORF Names:CG10572
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0015618 Cdk8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857981 – 454Cyclin-dependent kinase 8Add BLAST454

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VT57
PRIDEiQ9VT57

Expressioni

Developmental stagei

Expressed both maternally and zygotically during developmental periods of maximal cell division; most abundant in early embryos and low levels in larvae, pupae and adults.1 Publication

Gene expression databases

BgeeiFBgn0015618
ExpressionAtlasiQ9VT57 differential
GenevisibleiQ9VT57 DM

Interactioni

Subunit structurei

Component of the Cdk8 module of the Mediator complex, composed of CycC, Cdk8, kto and skd.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi64544, 47 interactors
DIPiDIP-38574N
IntActiQ9VT57, 5 interactors
MINTiQ9VT57
STRINGi7227.FBpp0076098

Structurei

3D structure databases

ProteinModelPortaliQ9VT57
SMRiQ9VT57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 335Protein kinasePROSITE-ProRule annotationAdd BLAST315

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi328 – 451Gln-richPROSITE-ProRule annotationAdd BLAST124

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0666 Eukaryota
ENOG410XPPA LUCA
GeneTreeiENSGT00530000064012
InParanoidiQ9VT57
KOiK02208
OMAiKSIYMVF
OrthoDBiEOG091G0812
PhylomeDBiQ9VT57

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VT57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYDFKMKTQ IERTKVEDLF NYEGCKVGRG TYGHVYKAKW KETSDGKEYA
60 70 80 90 100
LKQIDGTGLS MSACREIALL RELKHQNVIT LIRVFLSHND RKVFLLIDYA
110 120 130 140 150
EHDLWHIIKF HRAAKATKKQ VVVPRGMVKS LLYQILDGIH YLHSNWVLHR
160 170 180 190 200
DLKPANILVM GDGNERGRVK IADMGFARLF NAPLKPLADL DPVVVTFWYR
210 220 230 240 250
APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED IKTSNPYHHD
260 270 280 290 300
QLDRIFNVMG FPQDKDWEDI KKMPEHHTLT KDFKRSTYST CSLAKYMERH
310 320 330 340 350
KIKPDSKAFH LLQKLLLMDP NKRITSEQAM QDQYFQEEPQ PTQDVFAGCP
360 370 380 390 400
IPYPKREFLT DDDQEDKSDN KRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ
410 420 430 440 450
MNAEPNAKRV RLSGAGNQQD FHHQQQQQQQ QQQQQQQQQQ QMMFNQQQNF

QRFN
Length:454
Mass (Da):53,682
Last modified:March 1, 2001 - v2
Checksum:iD73F6219E2A03C98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9T → A in AAB38385 (PubMed:8730095).Curated1
Sequence conflicti15K → N in AAB38385 (PubMed:8730095).Curated1
Sequence conflicti37K → R in AAB38385 (PubMed:8730095).Curated1
Sequence conflicti40W → R in AAB38385 (PubMed:8730095).Curated1
Sequence conflicti70L → W in AAB38385 (PubMed:8730095).Curated1
Sequence conflicti185K → R in AAM50971 (PubMed:12537569).Curated1
Sequence conflicti321N → I in AAB38385 (PubMed:8730095).Curated1
Sequence conflicti336Q → R in AAB38385 (PubMed:8730095).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33015 mRNA Translation: AAB38385.1
AE014296 Genomic DNA Translation: AAF50197.2
AY119111 mRNA Translation: AAM50971.1
RefSeqiNP_536735.2, NM_080487.4

Genome annotation databases

EnsemblMetazoaiFBtr0076369; FBpp0076098; FBgn0015618
GeneIDi39157
KEGGidme:Dmel_CG10572

Similar proteinsi

Entry informationi

Entry nameiCDK8_DROME
AccessioniPrimary (citable) accession number: Q9VT57
Secondary accession number(s): P91642, Q8MS41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health