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Q9VT57

- CDK8_DROME

UniProt

Q9VT57 - CDK8_DROME

Protein

Cyclin-dependent kinase 8

Gene

Cdk8

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May phosphorylate the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Required for leg and eye development and macrochaete specification or differentiation.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity1 Publication
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521ATPBy similarityPROSITE-ProRule annotation
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi27 – 359ATPBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. protein binding Source: UniProtKB
    4. protein serine/threonine kinase activity Source: FlyBase
    5. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC
    6. RNA polymerase II transcription cofactor activity Source: FlyBase

    GO - Biological processi

    1. axon guidance Source: FlyBase
    2. chaeta development Source: FlyBase
    3. G1/S transition of mitotic cell cycle Source: UniProtKB
    4. imaginal disc-derived leg segmentation Source: FlyBase
    5. mitotic cell cycle Source: FlyBase
    6. mitotic G2 DNA damage checkpoint Source: FlyBase
    7. protein phosphorylation Source: UniProtKB
    8. regulation of transcription from RNA polymerase II promoter Source: GOC
    9. sex comb development Source: FlyBase
    10. snRNA 3'-end processing Source: FlyBase
    11. transcription initiation from RNA polymerase II promoter Source: FlyBase

    Keywords - Molecular functioni

    Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_180846. PPARA activates gene expression.
    REACT_181685. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
    SignaLinkiQ9VT57.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 8 (EC:2.7.11.22, EC:2.7.11.23)
    Alternative name(s):
    Cell division protein kinase 8
    Short name:
    DmCdk8
    Mediator complex subunit Cdk8
    Mediator of RNA polymerase II transcription subunit Cdk8
    Gene namesi
    Name:Cdk8
    ORF Names:CG10572
    OrganismiDrosophila melanogaster (Fruit fly)Imported
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0015618. Cdk8.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. mediator complex Source: FlyBase
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 454454Cyclin-dependent kinase 8PRO_0000085798Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9VT57.

    Expressioni

    Developmental stagei

    Expressed both maternally and zygotically during developmental periods of maximal cell division; most abundant in early embryos and low levels in larvae, pupae and adults.1 Publication

    Gene expression databases

    BgeeiQ9VT57.

    Interactioni

    Subunit structurei

    Component of the Cdk8 module of the Mediator complex, composed of CycC, Cdk8, kto and skd.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CycCP250082EBI-163640,EBI-195485

    Protein-protein interaction databases

    BioGridi64544. 32 interactions.
    DIPiDIP-38574N.
    IntActiQ9VT57. 3 interactions.
    MINTiMINT-4329200.
    STRINGi7227.FBpp0076098.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VT57.
    SMRiQ9VT57. Positions 1-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 335315Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi328 – 451124Gln-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000064012.
    InParanoidiQ9VT57.
    KOiK02208.
    OMAiNVITLIR.
    OrthoDBiEOG76739K.
    PhylomeDBiQ9VT57.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9VT57-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYDFKMKTQ IERTKVEDLF NYEGCKVGRG TYGHVYKAKW KETSDGKEYA    50
    LKQIDGTGLS MSACREIALL RELKHQNVIT LIRVFLSHND RKVFLLIDYA 100
    EHDLWHIIKF HRAAKATKKQ VVVPRGMVKS LLYQILDGIH YLHSNWVLHR 150
    DLKPANILVM GDGNERGRVK IADMGFARLF NAPLKPLADL DPVVVTFWYR 200
    APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED IKTSNPYHHD 250
    QLDRIFNVMG FPQDKDWEDI KKMPEHHTLT KDFKRSTYST CSLAKYMERH 300
    KIKPDSKAFH LLQKLLLMDP NKRITSEQAM QDQYFQEEPQ PTQDVFAGCP 350
    IPYPKREFLT DDDQEDKSDN KRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ 400
    MNAEPNAKRV RLSGAGNQQD FHHQQQQQQQ QQQQQQQQQQ QMMFNQQQNF 450
    QRFN 454
    Length:454
    Mass (Da):53,682
    Last modified:March 1, 2001 - v2
    Checksum:iD73F6219E2A03C98
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91T → A in AAB38385. (PubMed:8730095)Curated
    Sequence conflicti15 – 151K → N in AAB38385. (PubMed:8730095)Curated
    Sequence conflicti37 – 371K → R in AAB38385. (PubMed:8730095)Curated
    Sequence conflicti40 – 401W → R in AAB38385. (PubMed:8730095)Curated
    Sequence conflicti70 – 701L → W in AAB38385. (PubMed:8730095)Curated
    Sequence conflicti185 – 1851K → R in AAM50971. (PubMed:12537569)Curated
    Sequence conflicti321 – 3211N → I in AAB38385. (PubMed:8730095)Curated
    Sequence conflicti336 – 3361Q → R in AAB38385. (PubMed:8730095)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33015 mRNA. Translation: AAB38385.1.
    AE014296 Genomic DNA. Translation: AAF50197.2.
    AY119111 mRNA. Translation: AAM50971.1.
    RefSeqiNP_536735.2. NM_080487.3.
    UniGeneiDm.5731.

    Genome annotation databases

    EnsemblMetazoaiFBtr0076369; FBpp0076098; FBgn0015618.
    GeneIDi39157.
    KEGGidme:Dmel_CG10572.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33015 mRNA. Translation: AAB38385.1 .
    AE014296 Genomic DNA. Translation: AAF50197.2 .
    AY119111 mRNA. Translation: AAM50971.1 .
    RefSeqi NP_536735.2. NM_080487.3.
    UniGenei Dm.5731.

    3D structure databases

    ProteinModelPortali Q9VT57.
    SMRi Q9VT57. Positions 1-359.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 64544. 32 interactions.
    DIPi DIP-38574N.
    IntActi Q9VT57. 3 interactions.
    MINTi MINT-4329200.
    STRINGi 7227.FBpp0076098.

    Proteomic databases

    PaxDbi Q9VT57.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0076369 ; FBpp0076098 ; FBgn0015618 .
    GeneIDi 39157.
    KEGGi dme:Dmel_CG10572.

    Organism-specific databases

    CTDi 1024.
    FlyBasei FBgn0015618. Cdk8.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000064012.
    InParanoidi Q9VT57.
    KOi K02208.
    OMAi NVITLIR.
    OrthoDBi EOG76739K.
    PhylomeDBi Q9VT57.

    Enzyme and pathway databases

    Reactomei REACT_180846. PPARA activates gene expression.
    REACT_181685. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
    SignaLinki Q9VT57.

    Miscellaneous databases

    GenomeRNAii 39157.
    NextBioi 812240.
    PROi Q9VT57.

    Gene expression databases

    Bgeei Q9VT57.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II."
      Leclerc V., Tassan J.-P., O'Farrell P.H., Nigg E.A., Leopold P.
      Mol. Biol. Cell 7:505-513(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
      Tissue: Embryo1 Publication.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley1 Publication.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley1 Publication.
      Tissue: Embryo1 Publication.
    5. "Distinct roles for Mediator Cdk8 module subunits in Drosophila development."
      Loncle N., Boube M., Joulia L., Boschiero C., Werner M., Cribbs D.L., Bourbon H.-M.
      EMBO J. 26:1045-1054(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CYCC; KTO AND SKD.

    Entry informationi

    Entry nameiCDK8_DROME
    AccessioniPrimary (citable) accession number: Q9VT57
    Secondary accession number(s): P91642, Q8MS41
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 2, 2004
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3