Q9VT52 (INSL3_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable insulin-like peptide 3
Short name=dILP3

Alternative name(s):
Insulin-related peptide 3

Cleaved into the following 2 chains:

1. Probable insulin-like peptide 3 A chain
2. Probable insulin-like peptide 3 B chain

Gene names

Name:	Ilp3
ORF Names:	CG14167

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subunit structure	Heterodimer of a B chain and an A chain linked by two disulfide bonds By similarity.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed at a high level in seven cells of each larval brain hemisphere that may correspond to neurosecretory cells. Ref.3
Developmental stage	Expressed in the larva, but not in the embryo. Ref.3
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	female mating behavior Inferred from mutant phenotype. Source: FlyBase insulin receptor signaling pathway Inferred from sequence or structural similarity Ref.3. Source: UniProtKB
Cellular component	extracellular region Traceable author statement. Source: Reactome
Molecular function	hormone activity Inferred from electronic annotation. Source: InterPro insulin receptor binding Inferred from sequence or structural similarity Ref.3. Source: UniProtKB transmembrane receptor protein tyrosine kinase activity Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 120	91	Probable insulin-like peptide 3		PRO_0000016193
	Peptide	30 – 48	19	Probable insulin-like peptide 3 B chain Potential		PRO_0000016194
	Propeptide	51 – 89	39	Connecting peptide Potential		PRO_0000016195
	Peptide	92 – 120	29	Probable insulin-like peptide 3 A chain Potential		PRO_0000016196
Amino acid modifications
	Disulfide bond	34 ↔ 101		Interchain (between B and A chains) By similarity
	Disulfide bond	46 ↔ 114		Interchain (between B and A chains) By similarity
	Disulfide bond	100 ↔ 105		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9VT52 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: FA834C8BAE1CE31B	FASTA	120	13,708
	10 20 30 40 50 60 MGIEMRCQDR RILLPSLLLL ILMIGGVQAT MKLCGRKLPE TLSKLCVYGF NAMTKRTLDP 70 80 90 100 110 120 VNFNQIDGFE DRSLLERLLS DSSVQMLKTR RLRDGVFDEC CLKSCTMDEV LRYCAAKPRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control." Brogiolo W., Stocker H., Ikeya T., Rintelen F., Fernandez R., Hafen E. Curr. Biol. 11:213-221(2001) [PubMed: 11250149] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014296 Genomic DNA. Translation: AAF50203.2.
RefSeq	NP_648360.2. NM_140103.3.
UniGene	Dm.27102.
3D structure databases
ProteinModelPortal	Q9VT52.
SMR	Q9VT52. Positions 29-56.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9VT52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076373; FBpp0076102; FBgn0044050.
GeneID	39151.
KEGG	dme:Dmel_CG14167.
NMPDR	fig|7227.3.peg.9155.
Organism-specific databases
CTD	39151.
FlyBase	FBgn0044050. Ilp3.
Phylogenomic databases
GeneTree	EMGT00050000016170.
InParanoid	Q9VT52.
OMA	CGTELPE.
OrthoDB	EOG4P2NJQ.
PhylomeDB	Q9VT52.
Enzyme and pathway databases
Reactome	REACT_115106. Signal Transduction. REACT_6313. Insulin receptor mediated signaling.
Gene expression databases
Bgee	Q9VT52.
GermOnline	CG14167. Drosophila melanogaster.
Family and domain databases
InterPro	IPR016179. Insulin-like. IPR022353. Insulin_CS. IPR022352. Insulin_family. [Graphical view]
Gene3D	G3DSA:1.10.100.10. Ins/IGF/relaxin. 1 hit.
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00276. INSULINFAMLY.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	812201.

Entry information

Entry name

INSL3_DROME

Accession

Primary (citable) accession number: Q9VT52

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 7, 2004
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project