Q9VT51 (INSL2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable insulin-like peptide 2
Short name=dILP2

Alternative name(s):
Insulin-related peptide 2

Cleaved into the following 2 chains:

1. Probable insulin-like peptide 2 A chain
2. Probable insulin-like peptide 2 B chain

Gene names

Name:	Ilp2
Synonyms:	IRP
ORF Names:	CG8167

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a role in regulating body size by increasing cell size and cell number of individual organs. Probably mediates its growth effects by acting as a ligand for the insulin receptor and transducing a signal via the Chico/PI3K/Akt(PKB) pathway. Ref.5
Subunit structure	Heterodimer of a B chain and an A chain linked by two disulfide bonds By similarity.
Subcellular location	Secreted Potential.
Tissue specificity	Broadly expressed at a low level in the embryonic mesoderm, beginning at stage 12. Expressed at a high level in the embryonic anterior midgut, with expression diminishing at late stage 16. Expressed at a low level in larval imaginal disks. Expressed at a high level in larval salivary glands and in seven cells of each larval brain hemisphere that may correspond to neurosecretory cells. Ref.5
Developmental stage	Expressed in the embryo and larva. Ref.5
Miscellaneous	Of the insulin-like peptides, Ilp2 is the closest homolog of human insulin.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Biological process	Growth regulation
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate utilization Inferred from mutant phenotype. Source: FlyBase determination of adult lifespan Inferred from mutant phenotype. Source: FlyBase female mating behavior Inferred from mutant phenotype. Source: FlyBase germ-line stem-cell niche homeostasis Inferred from mutant phenotype. Source: FlyBase growth Traceable author statement. Source: FlyBase insulin receptor signaling pathway Inferred from genetic interaction Ref.5. Source: UniProtKB larval feeding behavior Inferred from mutant phenotype. Source: FlyBase positive regulation of cell growth Inferred from mutant phenotype Ref.5. Source: UniProtKB positive regulation of cell proliferation Inferred from mutant phenotype Ref.5. Source: UniProtKB positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: FlyBase regulation of cell size Inferred from mutant phenotype Ref.5. Source: FlyBase regulation of growth rate Inferred from mutant phenotype. Source: FlyBase regulation of organ growth Inferred from mutant phenotype Ref.5. Source: UniProtKB triglyceride homeostasis Inferred from mutant phenotype. Source: FlyBase
Cellular component	extracellular region Non-traceable author statement Ref.5. Source: UniProtKB
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW hormone activity Inferred from electronic annotation. Source: InterPro insulin receptor binding Inferred from genetic interaction Ref.5. Source: UniProtKB transmembrane receptor protein tyrosine kinase activity Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 137	111	Probable insulin-like peptide 2		PRO_0000016189
	Peptide	27 – 50	24	Probable insulin-like peptide 2 B chain Potential		PRO_0000016190
	Propeptide	53 – 104	52	Connecting peptide Potential		PRO_0000016191
	Peptide	108 – 137	30	Probable insulin-like peptide 2 A chain Potential		PRO_0000016192
Amino acid modifications
	Disulfide bond	29 ↔ 119		Interchain (between B and A chains) By similarity
	Disulfide bond	41 ↔ 132		Interchain (between B and A chains) By similarity
	Disulfide bond	118 ↔ 123		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9VT51 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F5B2A357D7B653D6	FASTA	137	15,255
	10 20 30 40 50 60 MSKPLSFISM VAVILLASST VKLAQGTLCS EKLNEVLSMV CEEYNPVIPH KRAMPGADSD 70 80 90 100 110 120 LDALNPLQFV QEFEEEDNSI SEPLRSALFP GSYLGGVLNS LAEVRRRTRQ RQGIVERCCK 130 KSCDMKALRE YCSVVRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Neuropeptides and their precursors in the fruitfly, Drosophila melanogaster." Vanden Broeck J.J.M. Peptides 22:241-254(2001) [PubMed: 11179818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[5]	"An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control." Brogiolo W., Stocker H., Ikeya T., Rintelen F., Fernandez R., Hafen E. Curr. Biol. 11:213-221(2001) [PubMed: 11250149] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ291726 mRNA. Translation: CAC17605.1. AE014296 Genomic DNA. Translation: AAF50204.1. AY069095 mRNA. Translation: AAL39240.1.
RefSeq	NP_524012.1. NM_079288.2.
UniGene	Dm.2285.
3D structure databases
ProteinModelPortal	Q9VT51.
SMR	Q9VT51. Positions 24-133.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9VT51.
Proteomic databases
PRIDE	Q9VT51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076329; FBpp0076058; FBgn0036046.
GeneID	39150.
KEGG	dme:Dmel_CG8167.
NMPDR	fig|7227.3.peg.9154.
Organism-specific databases
CTD	39150.
FlyBase	FBgn0036046. Ilp2.
Phylogenomic databases
GeneTree	EMGT00050000016173.
InParanoid	Q9VT51.
OMA	RRTRQGI.
OrthoDB	EOG48W9JM.
PhylomeDB	Q9VT51.
Enzyme and pathway databases
Reactome	REACT_115106. Signal Transduction. REACT_6313. Insulin receptor mediated signaling.
Gene expression databases
Bgee	Q9VT51.
GermOnline	CG8167. Drosophila melanogaster.
Family and domain databases
InterPro	IPR016179. Insulin-like. IPR022353. Insulin_CS. IPR022352. Insulin_family. [Graphical view]
Gene3D	G3DSA:1.10.100.10. Ins/IGF/relaxin. 2 hits.
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00276. INSULINFAMLY.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	812196.

Entry information

Entry name

INSL2_DROME

Accession

Primary (citable) accession number: Q9VT51

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project