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Protein

Alpha-tubulin N-acetyltransferase 1

Gene

CG3967

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released.UniRule annotation

Catalytic activityi

Acetyl-CoA + [alpha-tubulin]-L-lysine = CoA + [alpha-tubulin]-N(6)-acetyl-L-lysine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Crucial for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • alpha-tubulin acetylation Source: GO_Central
  • behavioral response to ethanol Source: FlyBase
  • neuron development Source: UniProtKB-HAMAP
  • regulation of microtubule cytoskeleton organization Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-tubulin N-acetyltransferase 1UniRule annotation (EC:2.3.1.108UniRule annotation)
Short name:
Alpha-TAT 1UniRule annotation
Short name:
TAT 1UniRule annotation
Alternative name(s):
Acetyltransferase mec-17 homolog 1UniRule annotation
Gene namesi
ORF Names:CG3967
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0035989. CG3967.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003480691 – 461Alpha-tubulin N-acetyltransferase 1Add BLAST461

Proteomic databases

PaxDbiQ9VSY4.
PRIDEiQ9VSY4.

Expressioni

Gene expression databases

BgeeiFBgn0035989.
ExpressionAtlasiQ9VSY4. baseline.
GenevisibleiQ9VSY4. DM.

Interactioni

Protein-protein interaction databases

BioGridi64480. 1 interactor.
IntActiQ9VSY4. 1 interactor.
MINTiMINT-1031519.
STRINGi7227.FBpp0076209.

Structurei

3D structure databases

ProteinModelPortaliQ9VSY4.
SMRiQ9VSY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 189N-acetyltransferaseUniRule annotationAdd BLAST188

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 136Acetyl-CoA bindingUniRule annotationAdd BLAST14
Regioni159 – 168Acetyl-CoA bindingUniRule annotation10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi318 – 359Pro-richAdd BLAST42

Sequence similaritiesi

Belongs to the acetyltransferase ATAT1 family.UniRule annotation
Contains 1 N-acetyltransferase domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG4601. Eukaryota.
ENOG4111Q8H. LUCA.
GeneTreeiENSGT00390000008276.
InParanoidiQ9VSY4.
KOiK19573.
OMAiGRELFEH.
OrthoDBiEOG091G0F8N.
PhylomeDBiQ9VSY4.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
HAMAPiMF_03130. mec17. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR007965. GNAT_ATAT.
[Graphical view]
PfamiPF05301. Acetyltransf_16. 1 hit.
[Graphical view]
PROSITEiPS51730. GNAT_ATAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9VSY4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVEFRFDIKP LFAQPIIKVT SNLLPNTFRG DRRQCLDATS KMTEIIDQLG
60 70 80 90 100
QLSATSQGLS KPVTTAQRLR MSDNQTIYLL ADNEAGHNGA VLGLLKVGTK
110 120 130 140 150
NLYLFDEAGK TRMVEQTPSI LDFYVHESRQ RAGLGKRLFQ TMLNEEQWTA
160 170 180 190 200
RKCSVDRPSE KLLSFLSKHY GLKRIIPQAN NFVLYEGFFN DGESGNGGGN
210 220 230 240 250
GHANGTPNGL HITNSPNTHL FGATYLGEDS NQRRGSQQQT TPNARLQQIT
260 270 280 290 300
QISPSGRYGA KRPTCSMAEI IHAGNSKGGN GNGSAEANSG NGNHDIPEIA
310 320 330 340 350
EQLQRQSLAD LEANSYEPEP EVEPEPEPEP EPEPEPEVIT PPSPPPKSHT
360 370 380 390 400
PTPPSVRSPE VAESIVGDNR RAPAFQLSKQ HTGMKNRSFG VGMAVMPSSK
410 420 430 440 450
MEFDQMERED FGVVKINRPP GHEVTSPGQD NTDAMSTVSS GGGGLTDQGY
460
YDLKFYHNKL W
Length:461
Mass (Da):50,560
Last modified:October 1, 2002 - v2
Checksum:i567F1C6B08EB9B68
GO
Isoform 2 (identifier: Q9VSY4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     289-461: SGNGNHDIPE...DLKFYHNKLW → RIK

Note: No experimental confirmation available.
Show »
Length:291
Mass (Da):32,015
Checksum:iFF6C22538588A244
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035094289 – 461SGNGN…HNKLW → RIK in isoform 2. CuratedAdd BLAST173

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50275.2.
AE014296 Genomic DNA. Translation: AAF50276.1.
AE014296 Genomic DNA. Translation: AAN11967.1.
AE014296 Genomic DNA. Translation: AAN11968.1.
AE014296 Genomic DNA. Translation: AAN11969.1.
AY061003 mRNA. Translation: AAL28551.1.
RefSeqiNP_001261628.1. NM_001274699.1. [Q9VSY4-2]
NP_648309.1. NM_140052.2. [Q9VSY4-1]
NP_648310.1. NM_140053.2. [Q9VSY4-1]
NP_729487.1. NM_170630.2. [Q9VSY4-1]
NP_729488.1. NM_170631.1. [Q9VSY4-1]
NP_729489.1. NM_170632.2. [Q9VSY4-2]
UniGeneiDm.7851.

Genome annotation databases

EnsemblMetazoaiFBtr0076481; FBpp0076209; FBgn0035989. [Q9VSY4-1]
FBtr0076482; FBpp0076210; FBgn0035989. [Q9VSY4-1]
FBtr0076483; FBpp0076211; FBgn0035989. [Q9VSY4-1]
FBtr0076484; FBpp0076212; FBgn0035989. [Q9VSY4-1]
GeneIDi39086.
KEGGidme:Dmel_CG3967.
UCSCiCG3967-RA. d. melanogaster. [Q9VSY4-1]
CG3967-RB. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50275.2.
AE014296 Genomic DNA. Translation: AAF50276.1.
AE014296 Genomic DNA. Translation: AAN11967.1.
AE014296 Genomic DNA. Translation: AAN11968.1.
AE014296 Genomic DNA. Translation: AAN11969.1.
AY061003 mRNA. Translation: AAL28551.1.
RefSeqiNP_001261628.1. NM_001274699.1. [Q9VSY4-2]
NP_648309.1. NM_140052.2. [Q9VSY4-1]
NP_648310.1. NM_140053.2. [Q9VSY4-1]
NP_729487.1. NM_170630.2. [Q9VSY4-1]
NP_729488.1. NM_170631.1. [Q9VSY4-1]
NP_729489.1. NM_170632.2. [Q9VSY4-2]
UniGeneiDm.7851.

3D structure databases

ProteinModelPortaliQ9VSY4.
SMRiQ9VSY4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64480. 1 interactor.
IntActiQ9VSY4. 1 interactor.
MINTiMINT-1031519.
STRINGi7227.FBpp0076209.

Proteomic databases

PaxDbiQ9VSY4.
PRIDEiQ9VSY4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0076481; FBpp0076209; FBgn0035989. [Q9VSY4-1]
FBtr0076482; FBpp0076210; FBgn0035989. [Q9VSY4-1]
FBtr0076483; FBpp0076211; FBgn0035989. [Q9VSY4-1]
FBtr0076484; FBpp0076212; FBgn0035989. [Q9VSY4-1]
GeneIDi39086.
KEGGidme:Dmel_CG3967.
UCSCiCG3967-RA. d. melanogaster. [Q9VSY4-1]
CG3967-RB. d. melanogaster.

Organism-specific databases

FlyBaseiFBgn0035989. CG3967.

Phylogenomic databases

eggNOGiKOG4601. Eukaryota.
ENOG4111Q8H. LUCA.
GeneTreeiENSGT00390000008276.
InParanoidiQ9VSY4.
KOiK19573.
OMAiGRELFEH.
OrthoDBiEOG091G0F8N.
PhylomeDBiQ9VSY4.

Miscellaneous databases

ChiTaRSiCG3967. fly.
GenomeRNAii39086.
PROiQ9VSY4.

Gene expression databases

BgeeiFBgn0035989.
ExpressionAtlasiQ9VSY4. baseline.
GenevisibleiQ9VSY4. DM.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
HAMAPiMF_03130. mec17. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR007965. GNAT_ATAT.
[Graphical view]
PfamiPF05301. Acetyltransf_16. 1 hit.
[Graphical view]
PROSITEiPS51730. GNAT_ATAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATAT1_DROME
AccessioniPrimary (citable) accession number: Q9VSY4
Secondary accession number(s): Q95S14, Q9VSY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.