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Protein

Pyrimidodiazepine synthase

Gene

se

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the conversion of 2-amino-4-oxo-6-pyruvoyl-5,6,7,8-tetrahydropteridine (6-PTP; also named 6-pyruvoyltetrahydropterin) to 2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1,4]diazepin-4-one (pyrimidodiazepine or PDA), a key intermediate in red eye pigment drosopterin biosynthesis.2 Publications

Catalytic activityi

2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei30 – 301NucleophileBy similarity
Binding sitei57 – 571GlutathioneBy similarity
Binding sitei70 – 701Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  • glutathione dehydrogenase (ascorbate) activity Source: FlyBase
  • glutathione transferase activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase
  • pyrimidodiazepine synthase activity Source: FlyBase
  • transferase activity, transferring sulfur-containing groups Source: FlyBase

GO - Biological processi

  • eye pigment biosynthetic process Source: FlyBase
  • glutathione metabolic process Source: FlyBase
  • pteridine biosynthetic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18458.
ReactomeiR-DME-156581. Methylation.
R-DME-156590. Glutathione conjugation.
R-DME-196836. Vitamin C (ascorbate) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrimidodiazepine synthaseCurated (EC:1.5.4.11 Publication)
Alternative name(s):
Protein sepia1 Publication
Gene namesi
Name:seImported
ORF Names:CG6781Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0086348. se.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Sepia eye color, due to defects in 2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1,4]diazepin-4-one (pyrimidodiazepine or PDA) synthesis, a key intermediate in red eye pigment drosopterin biosynthesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243Pyrimidodiazepine synthasePRO_0000430582Add
BLAST

Proteomic databases

PaxDbiQ9VSL3.
PRIDEiQ9VSL3.

Expressioni

Developmental stagei

Appears only at the late pupal stage; expressed at high level. Also expressed in the early adult (0-2 days old) at lower level. Expressed exclusively in the adult head.1 Publication

Gene expression databases

BgeeiFBgn0086348.
ExpressionAtlasiQ9VSL3. differential.
GenevisibleiQ9VSL3. DM.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi64383. 1 interaction.
IntActiQ9VSL3. 1 interaction.
MINTiMINT-321360.
STRINGi7227.FBpp0076349.

Structurei

3D structure databases

ProteinModelPortaliQ9VSL3.
SMRiQ9VSL3. Positions 22-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 10283GST N-terminalSequence analysisAdd
BLAST
Domaini107 – 230124GST C-terminalSequence analysisAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni86 – 872Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Omega family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0406. Eukaryota.
ENOG410XSIX. LUCA.
GeneTreeiENSGT00390000005479.
InParanoidiQ9VSL3.
KOiK00310.
OMAiWSGLDIY.
OrthoDBiEOG091G0IEA.
PhylomeDBiQ9VSL3.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR005442. GST_omega.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01625. GSTRNSFRASEO.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VSL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNGRHLAKG SPMPDVPEDG ILRLYSMRFC PFAQRVHLVL DAKQIPYHSI
60 70 80 90 100
YINLTDKPEW LLEKNPQGKV PALEIVREPG PPVLTESLLI CEYLDEQYPL
110 120 130 140 150
RPLYPRDPLK KVQDKLLIER FRAVLGAFFK ASDGGDLEPF WSGLDIYERE
160 170 180 190 200
LARRGTEFFG GEQTGILDYM IWPWCERLEL LKLQRGEDYN YDQSRFPQLT
210 220 230 240
LWLERMKRDP AVMAFYMEAE VQAEFLRTRS LGRPNYNLLV KDA
Length:243
Mass (Da):28,413
Last modified:May 1, 2000 - v1
Checksum:iA6B651F36487A5AE
GO

Sequence cautioni

The sequence ACZ02425 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence ACZ02430 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50405.1.
AY070667 mRNA. Translation: AAL48138.1.
GQ351317 Genomic DNA. Translation: ACZ02425.1. Sequence problems.
GQ351318 Genomic DNA. Translation: ACZ02430.1. Sequence problems.
RefSeqiNP_648235.1. NM_139978.4.
UniGeneiDm.6327.

Genome annotation databases

EnsemblMetazoaiFBtr0076623; FBpp0076349; FBgn0086348.
GeneIDi38973.
KEGGidme:Dmel_CG6781.
UCSCiCG6781-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50405.1.
AY070667 mRNA. Translation: AAL48138.1.
GQ351317 Genomic DNA. Translation: ACZ02425.1. Sequence problems.
GQ351318 Genomic DNA. Translation: ACZ02430.1. Sequence problems.
RefSeqiNP_648235.1. NM_139978.4.
UniGeneiDm.6327.

3D structure databases

ProteinModelPortaliQ9VSL3.
SMRiQ9VSL3. Positions 22-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64383. 1 interaction.
IntActiQ9VSL3. 1 interaction.
MINTiMINT-321360.
STRINGi7227.FBpp0076349.

Proteomic databases

PaxDbiQ9VSL3.
PRIDEiQ9VSL3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0076623; FBpp0076349; FBgn0086348.
GeneIDi38973.
KEGGidme:Dmel_CG6781.
UCSCiCG6781-RA. d. melanogaster.

Organism-specific databases

CTDi38973.
FlyBaseiFBgn0086348. se.

Phylogenomic databases

eggNOGiKOG0406. Eukaryota.
ENOG410XSIX. LUCA.
GeneTreeiENSGT00390000005479.
InParanoidiQ9VSL3.
KOiK00310.
OMAiWSGLDIY.
OrthoDBiEOG091G0IEA.
PhylomeDBiQ9VSL3.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18458.
ReactomeiR-DME-156581. Methylation.
R-DME-156590. Glutathione conjugation.
R-DME-196836. Vitamin C (ascorbate) metabolism.

Miscellaneous databases

GenomeRNAii38973.
PROiQ9VSL3.

Gene expression databases

BgeeiFBgn0086348.
ExpressionAtlasiQ9VSL3. differential.
GenevisibleiQ9VSL3. DM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR005442. GST_omega.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01625. GSTRNSFRASEO.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEPIA_DROME
AccessioniPrimary (citable) accession number: Q9VSL3
Secondary accession number(s): D1KS67, D1KS72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.