Reviewed, UniProtKB/Swiss-Prot Q9VSH4 (CPSF6_DROME)

Names and origin

Protein names

Recommended name:
    Cleavage and polyadenylation specificity factor subunit CG7185

Gene names

ORF Names:

CG7185

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in pre-mRNA 3' processing By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RRM CPSF6/7 family. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW regulation of alternative nuclear mRNA splicing, via spliceosome Inferred from mutant phenotype. Source: FlyBase
Cellular component	nucleus Inferred by curator. Source: FlyBase
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VHN3	1	EBI-193310,EBI-141029
	Q9VMH8	1	EBI-193310,EBI-108827
	Q9VSY2	1	EBI-193310,EBI-172334
Cbp20	Q9V3L6	1	EBI-193310,EBI-85006

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 652	652	Cleavage and polyadenylation specificity factor subunit CG7185		PRO_0000372849
Regions
	Domain	93 – 173	81	RRM
	Compositional bias	26 – 76	51	Gly-rich
	Compositional bias	195 – 392	198	Gly-rich
	Compositional bias	521 – 651	131	Arg-rich
Amino acid modifications
	Modified residue	596	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	Q9VSH4-1 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 96B7410A48AD3F6C	FASTA	652	71,094
	10 20 30 40 50 60 MADVVLDLYA EDLDKDFAGQ AQDEFGGDGV DLYDDIGGPT ESAASGGGGG GTPSADGAAG 70 80 90 100 110 120 PGSGEPGERN SGGPNGVYHQ SSGSLTPTMN RRYQLYVGNL TWWTTDQDIA NSLRDIGVSD 130 140 150 160 170 180 LQEVKFFENR ANGQSKGFSV ISLGSESSLR AVLDQLPKKE MHGQAPVVTY PSKQALTQFE 190 200 210 220 230 240 SLQKTRPVPP PQQNGPPRGP APPSMGGGPM PTGHPGGPQG GGPPGHPPRG MNSIMQPGQY 250 260 270 280 290 300 RPQHMSQVPQ VGGPNSGPPR MQPPMHPQGG LMGNQQPPPR YPSAQGQWPG QRPGGPRPGP 310 320 330 340 350 360 PNGPPQRPMF QGGPMGMPVR GPAGPDWRRP PMHGGFPPQG PPRGLPPAPG PGGPHGAPAP 370 380 390 400 410 420 HVNPAFFNQP GGPAQHPGMG GPPHGAPGPQ PGMNMPPQQG MNMTPQHGPP PQFAQHGPRG 430 440 450 460 470 480 PWPPPQGKPP GPFPDPQQMG PQLTEVEFEE VMSRNRTVSS SAIARAVSDA AAGEYSSAIE 490 500 510 520 530 540 TLVTAISLIK QSKVAHDERC KILISSLQDT LHGIEAKSYN RRERSRSRER SHRSRQRRER 550 560 570 580 590 600 STSRYRERSR ERERDRDRER ERDGGSYRER SRSRERERQA PDHYRDDSRS VRPRKSPEPV 610 620 630 640 650 VAEAAEAPSS KRYYEDRERY RSSDRERRDR DRDRDRERER DRDRREEHRS RH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014296 Genomic DNA. Translation: AAF50445.2. AY058563 mRNA. Translation: AAL13792.1.
RefSeq	NP_648206.1.
UniGene	Dm.887
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VSH4. 7 interactions.
Genome annotation databases
Ensembl	FBgn0035872. Drosophila melanogaster. [Contig view]
GeneID	38937.
KEGG	dme:Dmel_CG7185.
NMPDR	fig|7227.3.peg.8836.
Organism-specific databases
FlyBase	FBgn0035872. CG7185.
Phylogenomic databases
HOGENOM	Q9VSH4.
OMA	Q9VSH4. EMQSRKS.
Gene expression databases
ArrayExpress	Q9VSH4.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR000504. RRM_RNP1. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	811101.

Entry information

Entry name

CPSF6_DROME

Accession

Primary (citable) accession number: Q9VSH4
Secondary accession number(s): Q95TS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 1, 2002
Last modified:	July 7, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project