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Protein

Nedd8-conjugating enzyme Ubc12

Gene

Ubc12

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin-like protein Nedd8 from the Uba3-APP-BP1 E1 complex and catalyzes its covalent attachment to other proteins. Required for Cul1 and Cul3 neddylation. Negatively regulates full-length ci stability and hedgehog signaling.1 Publication

Catalytic activityi

ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular protein modification process Source: UniProtKB
  • negative regulation of smoothened signaling pathway Source: FlyBase
  • protein neddylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
SignaLinkiQ9VSF3.
UniPathwayiUPA00885.

Names & Taxonomyi

Protein namesi
Recommended name:
Nedd8-conjugating enzyme Ubc12 (EC:6.3.2.-)
Alternative name(s):
Nedd8 carrier protein
Nedd8 protein ligase
Gene namesi
Name:Ubc12
ORF Names:CG7375
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0035853. Ubc12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 2322Missing : Abolishes neddylation E2 activity. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Nedd8-conjugating enzyme Ubc12PRO_0000082492Add
BLAST

Proteomic databases

PaxDbiQ9VSF3.
PRIDEiQ9VSF3.

Expressioni

Tissue specificityi

Expressed in the wing disk.1 Publication

Gene expression databases

BgeeiFBgn0035853.
ExpressionAtlasiQ9VSF3. differential.
GenevisibleiQ9VSF3. DM.

Interactioni

Subunit structurei

Interacts with Uba3.By similarity

Protein-protein interaction databases

BioGridi64336. 17 interactions.
IntActiQ9VSF3. 4 interactions.
MINTiMINT-295740.
STRINGi7227.FBpp0304449.

Structurei

3D structure databases

ProteinModelPortaliQ9VSF3.
SMRiQ9VSF3. Positions 24-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 74Interaction with Uba3By similarity
Regioni24 – 5431Interaction with Uba3By similarityAdd
BLAST

Domaini

Both the N-terminal docking peptide and the catalytic core domain must bind the Uba3-Ula1 complex simultaneously for optimal transfer of Nedd8.By similarity

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0420. Eukaryota.
ENOG410XS81. LUCA.
GeneTreeiENSGT00630000089859.
InParanoidiQ9VSF3.
KOiK10579.
OMAiHEPPKVR.
OrthoDBiEOG091G0N8A.
PhylomeDBiQ9VSF3.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VSF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKLFTLKQQ KKDGEQKGSQ QKKASAAQLR IQKDINELNL PNTCATDFPD
60 70 80 90 100
PNDLLNFKLI ISPDEGFYRD GRFVFNFRVG SNYPHEPPKV KCATQVYHPN
110 120 130 140 150
IDLDGNVCLN ILREDWNPVL NINSIVYGLQ FLFLEPNPED PLNKEAADVL
160 170 180
QTNRRQFENN VKKAMRGGCV GETYFECCLL K
Length:181
Mass (Da):20,738
Last modified:May 1, 2000 - v1
Checksum:iB8580A25E1E7543D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50468.1.
AY118545 mRNA. Translation: AAM49914.1.
RefSeqiNP_001261567.1. NM_001274638.1.
NP_001261568.1. NM_001274639.1.
NP_648187.1. NM_139930.2.
UniGeneiDm.11643.

Genome annotation databases

EnsemblMetazoaiFBtr0076722; FBpp0076445; FBgn0035853.
FBtr0332138; FBpp0304448; FBgn0035853.
FBtr0332139; FBpp0304449; FBgn0035853.
GeneIDi38916.
KEGGidme:Dmel_CG7375.
UCSCiCG7375-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50468.1.
AY118545 mRNA. Translation: AAM49914.1.
RefSeqiNP_001261567.1. NM_001274638.1.
NP_001261568.1. NM_001274639.1.
NP_648187.1. NM_139930.2.
UniGeneiDm.11643.

3D structure databases

ProteinModelPortaliQ9VSF3.
SMRiQ9VSF3. Positions 24-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64336. 17 interactions.
IntActiQ9VSF3. 4 interactions.
MINTiMINT-295740.
STRINGi7227.FBpp0304449.

Proteomic databases

PaxDbiQ9VSF3.
PRIDEiQ9VSF3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0076722; FBpp0076445; FBgn0035853.
FBtr0332138; FBpp0304448; FBgn0035853.
FBtr0332139; FBpp0304449; FBgn0035853.
GeneIDi38916.
KEGGidme:Dmel_CG7375.
UCSCiCG7375-RA. d. melanogaster.

Organism-specific databases

CTDi38916.
FlyBaseiFBgn0035853. Ubc12.

Phylogenomic databases

eggNOGiKOG0420. Eukaryota.
ENOG410XS81. LUCA.
GeneTreeiENSGT00630000089859.
InParanoidiQ9VSF3.
KOiK10579.
OMAiHEPPKVR.
OrthoDBiEOG091G0N8A.
PhylomeDBiQ9VSF3.

Enzyme and pathway databases

UniPathwayiUPA00885.
ReactomeiR-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
SignaLinkiQ9VSF3.

Miscellaneous databases

GenomeRNAii38916.
PROiQ9VSF3.

Gene expression databases

BgeeiFBgn0035853.
ExpressionAtlasiQ9VSF3. differential.
GenevisibleiQ9VSF3. DM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC12_DROME
AccessioniPrimary (citable) accession number: Q9VSF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.