Q9VSF2 (MED24_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 24

Alternative name(s):
Mediator complex subunit 24
dMED24
dTRAP100

Gene names

Name:	MED24
Synonyms:	Trap100
ORF Names:	CG7999

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	993 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for activated transcription of the MtnA, MtnB and MtnD genes. Ref.7
Subunit structure	Component of the Mediator complex, which includes at least CDK8, MED4, MED6, MED11, MED14, MED17, MED18, MED20, MED21, MED22, MED27, MED28, MED30 and MED31.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the Mediator complex subunit 24 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from mutant phenotype. Source: FlyBase dendrite morphogenesis Inferred from mutant phenotype. Source: FlyBase muscle organ development Inferred from mutant phenotype. Source: FlyBase positive regulation of programmed cell death Inferred from mutant phenotype. Source: FlyBase pupal development Inferred from mutant phenotype. Source: FlyBase salivary gland histolysis Inferred from mutant phenotype. Source: FlyBase
Cellular component	mediator complex Inferred from direct assay Ref.6. Source: UniProtKB
Molecular function	RNA polymerase II transcription cofactor activity Inferred from mutant phenotype Ref.6. Source: UniProtKB protein binding Inferred from physical interaction Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 993	993	Mediator of RNA polymerase II transcription subunit 24		PRO_0000305919
Amino acid modifications
	Modified residue	827	1	Phosphoserine Ref.8
	Modified residue	829	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	684	1	P → T in AAM52735. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9VSF2 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 63146DB943200E7A	FASTA	993	111,743
	10 20 30 40 50 60 MKENNKILQL IYVAWRERWT DSQWGINIKK VLPRGVSGDV YNLADCLMQQ ALIGSTANPL 70 80 90 100 110 120 VLNYLKHSLC AHLVSHAAVI RCIAKYDKLE RVYCITALLE FLASIVDGVT CRIKSEEAVL 130 140 150 160 170 180 PSSVVHLVYW LLQIFARTVQ HYELYGEISA EQSYMLDQTC VVIDRLSQQQ FLLSMLYVGC 190 200 210 220 230 240 HEELEICGRI RDKYATIKGS LTNSNFTLNA PQVEQQLQQL AFIEAKHLEM QPLNPPSTLE 250 260 270 280 290 300 KISCCVQPLL AVEVLLNPCK DTSYYVAELQ MLQRLKKYSN TRLFYEIIRA GFLTLSNVAD 310 320 330 340 350 360 TSPDTMWGAF MFFKMPHIIK QLHALQRIPG EQPPPADYIP ELVEALELLI EDNLLLDFMD 370 380 390 400 410 420 TKCSCNMIEF LLNDWTKQQL VNDVHVKKFA SQREAASQLL KKCDNGQQTP SNINFIKRAE 430 440 450 460 470 480 VPLSGVLKTL CTNKVQDMVN VLCQVPVGNS FELILSVATV EGRLKTFVSR LIQCNENSKP 490 500 510 520 530 540 VPGDLGKLCV IRSTLFDVSF LMLTSIVQTY GSDVVLSERG DSFFEKWVRE CMVERNKLKN 550 560 570 580 590 600 PRQILALCDD SIVDELLLSF SKPEASQLKP NNLSWQETCL NLPGVLHHVL IAWEQETLSS 610 620 630 640 650 660 ADVKSILDNI KRRLFSFSVC ATSFLCAYMY SVKETELLKP LNMIQQFLAP LTSEELSSQE 670 680 690 700 710 720 NAKERLALSY QIIRKMQHDV HPAPSTKSRL ISHSPLVEQF REVWRTVVDA GHLPVRAAQS 730 740 750 760 770 780 LESLLLAGGA AWLSTQLVEQ LLACKYTRDM SRTMDVVFAV MHLDIEKTTE ALLQYVVAPL 790 800 810 820 830 840 ILRRQGEDIN EPQSLVLARL CVYCIISCLE SRKGNGTSAL TAMKKRSRSH DDEELAANAA 850 860 870 880 890 900 KVRKVIGDGS DNSSDFTDTT TGAGLAALLG STSTSELRTT PLTLREPLQT SVQHIFAVFL 910 920 930 940 950 960 QFVSGDELSP KAVFVYQFIS LLVECGGERV APVLRLLPNG LVQQLLKVLV TDDIKVGLIS 970 980 990 RLYDLRIQAG RLSAVSDLCL WRNMQMARHS IHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transcriptional coactivators in Drosophila." Southworth J.W., Kennison J.A. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Novel Mediator proteins of the small Mediator complex in Drosophila SL2 cells." Gu J.-Y., Park J.M., Song E.J., Mizuguchi G., Yoon J.H., Kim-Ha J., Lee K.-J., Kim Y.-J. J. Biol. Chem. 277:27154-27161(2002) [PubMed: 12021283] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, INTERACTION WITH MED6 AND MED17.
[7]	"Coactivator cross-talk specifies transcriptional output." Marr M.T. II, Isogai Y., Wright K.J., Tjian R. Genes Dev. 20:1458-1469(2006) [PubMed: 16751183] [Abstract] Cited for: FUNCTION.
[8]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827 AND SER-829, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF289998 mRNA. Translation: AAG02463.1. AE014296 Genomic DNA. Translation: AAF50469.2. AY122223 mRNA. Translation: AAM52735.1. BT025232 mRNA. Translation: ABF17923.1.
RefSeq	NP_648185.1. NM_139928.4.
UniGene	Dm.3405.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9VSF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076694; FBpp0076417; FBgn0035851.
GeneID	38914.
KEGG	dme:Dmel_CG7999.
NMPDR	fig|7227.3.peg.8809.
Organism-specific databases
CTD	9862.
FlyBase	FBgn0035851. MED24.
Phylogenomic databases
eggNOG	inNOG05757.
GeneTree	EMGT00050000017270.
InParanoid	Q9VSF2.
OMA	FPSTGMD.
OrthoDB	EOG4V9S5J.
PhylomeDB	Q9VSF2.
Gene expression databases
Bgee	Q9VSF2.
Family and domain databases
InterPro	IPR021429. Mediator_Med24_N. [Graphical view]
KO	K15167.
Pfam	PF11277. Med24_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	810992.

Entry information

Entry name

MED24_DROME

Accession

Primary (citable) accession number: Q9VSF2
Secondary accession number(s): Q8MQZ3, Q9GYW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project