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Protein

Probable medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Gene

CG12262

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This enzyme is specific for acyl chain lengths of 4 to 16.By similarity

Catalytic activityi

A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein.

Cofactori

FADBy similarity

Pathwayi: mitochondrial fatty acid beta-oxidation

This protein is involved in the pathway mitochondrial fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway mitochondrial fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163Substrate; via carbonyl oxygenBy similarity1
Active sitei397Proton acceptorBy similarity1
Binding sitei398Substrate; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi154 – 163FADBy similarity10
Nucleotide bindingi187 – 189FADBy similarity3
Nucleotide bindingi302 – 304FADBy similarity3
Nucleotide bindingi312 – 313FADBy similarity2
Nucleotide bindingi370 – 374FADBy similarity5
Nucleotide bindingi399 – 401FADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-DME-77288 mitochondrial fatty acid beta-oxidation of unsaturated fatty acids
R-DME-77346 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA
R-DME-77348 Beta oxidation of octanoyl-CoA to hexanoyl-CoA
UniPathwayiUPA00660

Names & Taxonomyi

Protein namesi
Recommended name:
Probable medium-chain specific acyl-CoA dehydrogenase, mitochondrial (EC:1.3.8.7)
Short name:
MCAD
Gene namesi
ORF Names:CG12262
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0035811 CG12262

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionBy similarityAdd BLAST17
ChainiPRO_000000050818 – 419Probable medium-chain specific acyl-CoA dehydrogenase, mitochondrialAdd BLAST402

Proteomic databases

PaxDbiQ9VSA3
PRIDEiQ9VSA3

Expressioni

Gene expression databases

BgeeiFBgn0035811
ExpressionAtlasiQ9VSA3 differential
GenevisibleiQ9VSA3 DM

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi64295, 93 interactors
DIPiDIP-20518N
IntActiQ9VSA3, 8 interactors
STRINGi7227.FBpp0076520

Structurei

3D structure databases

ProteinModelPortaliQ9VSA3
SMRiQ9VSA3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni274 – 277Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0140 Eukaryota
COG1960 LUCA
GeneTreeiENSGT00760000119007
InParanoidiQ9VSA3
KOiK00249
OMAiCPPSKAF
OrthoDBiEOG091G04BS
PhylomeDBiQ9VSA3

Family and domain databases

CDDicd01157 MCAD, 1 hit
Gene3Di1.10.540.10, 1 hit
InterProiView protein in InterPro
IPR006089 Acyl-CoA_DH_CS
IPR006091 Acyl-CoA_Oxase/DH_cen-dom
IPR036250 AcylCo_DH-like_C
IPR009075 AcylCo_DH/oxidase_C
IPR013786 AcylCoA_DH/ox_N
IPR037069 AcylCoA_DH/ox_N_sf
IPR009100 AcylCoA_DH/oxidase_NM_dom
IPR034180 MCAD
PfamiView protein in Pfam
PF00441 Acyl-CoA_dh_1, 1 hit
PF02770 Acyl-CoA_dh_M, 1 hit
PF02771 Acyl-CoA_dh_N, 1 hit
SUPFAMiSSF47203 SSF47203, 1 hit
SSF56645 SSF56645, 1 hit
PROSITEiView protein in PROSITE
PS00072 ACYL_COA_DH_1, 1 hit
PS00073 ACYL_COA_DH_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9VSA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFLNKLAAP ALRQLVSQSR AYAAVSHVSP NGTSFALTED QLQLQELARK
60 70 80 90 100
FTREEIIPVA AQYDKSGEYP WPIIKKAWEL GLMNNHIPAD IGGLDLDVFT
110 120 130 140 150
TCLSAEELAY GCTGIMTALE ASGLGQTPVI LSGNKEQKKK YLGRLLEEPL
160 170 180 190 200
VAAYCVTEPG AGSDVSGIKT RAEKKGDEWV INGQKMWITN GGVANWYFVL
210 220 230 240 250
ARTNPDPKCP PSKAFTGFIV ERDSPGLTPG RKELNMGQRA SDTRGITFED
260 270 280 290 300
VRVPKENVLI GEGAGFKIAM GTFDKTRPPV AAGAVGLAQR CLDEALKYAL
310 320 330 340 350
ERKTFGVPIA YHQAVQFMLA DMAIGVETSR LAWRLSAWEI DQGRRNSYYA
360 370 380 390 400
SIAKCHAADM ANKIASDAVQ IFGGNGFNSE YPVEKLMRDA KIYQIYEGTS
410
QIQRLIISRN MYEAAKGQA
Length:419
Mass (Da):45,871
Last modified:May 1, 2000 - v1
Checksum:i0B1970302243C394
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AAF50524.1
AY089546 mRNA Translation: AAL90284.1
RefSeqiNP_648149.1, NM_139892.3
UniGeneiDm.879

Genome annotation databases

EnsemblMetazoaiFBtr0076808; FBpp0076520; FBgn0035811
GeneIDi38864
KEGGidme:Dmel_CG12262
UCSCiCG12262-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiACADM_DROME
AccessioniPrimary (citable) accession number: Q9VSA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health