Reviewed, UniProtKB/Swiss-Prot Q9VS24 (MELT_DROME)

Names and origin

Protein names

Recommended name:
    Protein melted

Gene names

Name:	melt
ORF Names:	CG8624

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	992 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Participates in fat metabolism regulation by recruiting FOXO and the TSC1-TSC2 complex to the cell membrane, which positively regulates TOR activity and negatively regulates the expression of FOXO target genes. Involved in R8 photoreceptor subtype specification. During early embryonic development, may be required for ectodermal patterning. Ref.4 Ref.5 Ref.6
Subunit structure	Interacts with TSC1 and FOXO. Ref.6
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Ref.6
Domain	The PH domain is required for membrane targeting.
Sequence similarities	Belongs to the MELT/VEPH family. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	R8 cell fate specification Ref.5 Inferred from mutant phenotype. Source: FlyBase ectoderm development Ref.4 Inferred from expression pattern. Source: FlyBase insulin receptor signaling pathway Ref.6 Inferred from mutant phenotype. Source: FlyBase lipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW peripheral nervous system development Ref.4 Inferred from mutant phenotype. Source: FlyBase sequestering of triglyceride Ref.6 Inferred from mutant phenotype. Source: FlyBase
Cellular component	plasma membrane Ref.6 Inferred from direct assay. Source: UniProtKB
Molecular function	phosphatidylinositol-5-phosphate binding Ref.6 Inferred from direct assay. Source: FlyBase protein binding Ref.6 Inferred from physical interaction. Source: UniProtKB specific transcriptional repressor activity Ref.5 Inferred from mutant phenotype. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VS24-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9VS24-2)

The sequence of this isoform differs from the canonical sequence as follows:
     385-385: K → KPV
     714-719: Missing.

Isoform C (identifier: Q9VS24-3)

The sequence of this isoform differs from the canonical sequence as follows:
     385-385: K → KPV

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 992	992	Protein melted		PRO_0000297961
Regions
	Domain	870 – 965	96	PH
Natural variations
	Alternative sequence	385	1	K → KPV in isoform B and isoform C.		VSP_027437
	Alternative sequence	714 – 719	6	Missing in isoform B.		VSP_027438
Experimental info
	Sequence conflict	403	1	V → A in AAF14808. Ref.4
	Sequence conflict	412	1	S → A in AAF14808. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified May 1, 2000. Version 1. Checksum: A1DC9F308B793412	FASTA	992	107,908
	10 20 30 40 50 60 MHELFNKVLA KRDLSRAGDL FSVPDADIVD DITEVLSEIS PIISHADYVK NNNDQSVVEI 70 80 90 100 110 120 CVTRVLSCIR ETKTAERYCA ALVDLLRTCL LWNLQPSGTT KEEPPHAKIA ADIISSIFLN 130 140 150 160 170 180 YDKKELMKIA LPVAVQFLPK GNRELSRNLA SYLSLAAIDH AGLLSPHTES VMESILSGNY 190 200 210 220 230 240 GLLRVLSQVY EVAPEAVTPH APLLMPLLPQ CDPQERMAVF QLYLLIVQKS PEVLESCVPQ 250 260 270 280 290 300 LCGSLLDSDT SSITMQILLK LSQHSPSLLV DHFEQLRLAA KTNPGTIPLC AQIMTTAGIG 310 320 330 340 350 360 SKETAQQALD FVLEHLPTQA LLQQEATRLC SAYPVLFTDK VLACVRQKNA ALSSQQDVGN 370 380 390 400 410 420 GLANKTSGGV TIVSLNSSSP SPPLKPVGAP AINTTIVQPA SVVSATPTPP PSSASAPIAS 430 440 450 460 470 480 STPVSAATPT PQHAGYTRRV KLGDSRSTGR LHPASNTHRS VTRLNVASGS VGGLHKSMTR 490 500 510 520 530 540 LSNSQINQQP GGSSNGNVVV QSGATPKTPT GLSNPPVTPV PPLSNNVVIT GHNRHGIPVT 550 560 570 580 590 600 SGGVTVTTSP SKVRPHSQGP STLLNSSTVL MKYSTDALNQ SVGSISIPQS AAVATLPSTQ 610 620 630 640 650 660 TQNAVSVHHA SPALPQSASN GHAKSVMKLP VNGNSEVIVS GPTTNVAPRR SDNTSRTLLN 670 680 690 700 710 720 ANSVMDQRMS TFEPYQIMRD PVQQFCEKNF NSIKSYMDEV SQHLPPPTRC SIEVSNEFAE 730 740 750 760 770 780 RRTKKVAKLH FACQIRGPHC LYSKTCFTMR TRNPKTWIHM MFLDFQVRHF VKEKCVLSTR 790 800 810 820 830 840 ESGISNLKNI WQILKCENRS FTELVTSQFP QVKDREILVN ELRHSGFLDV FEVSKTDKSN 850 860 870 880 890 900 PNCNELEYQW GCFLCNHPDK AVGFLNGSNQ PMIEGQLKEK KGKWRLFRRW RTRYFTLSGA 910 920 930 940 950 960 HLSCKGSSGG ESIDVNQIRS VKVSRGARNI PKAFEIFTAD QTLILKPKDG KNAEEWVQCL 970 980 990 SIVVAHSQAR DNPTAKTNSL PARSIGSSKP SF
	Isoform B. Checksum: B67970066F31F637	FASTA	988	107,457
	10 20 30 40 50 60 MHELFNKVLA KRDLSRAGDL FSVPDADIVD DITEVLSEIS PIISHADYVK NNNDQSVVEI 70 80 90 100 110 120 CVTRVLSCIR ETKTAERYCA ALVDLLRTCL LWNLQPSGTT KEEPPHAKIA ADIISSIFLN 130 140 150 160 170 180 YDKKELMKIA LPVAVQFLPK GNRELSRNLA SYLSLAAIDH AGLLSPHTES VMESILSGNY 190 200 210 220 230 240 GLLRVLSQVY EVAPEAVTPH APLLMPLLPQ CDPQERMAVF QLYLLIVQKS PEVLESCVPQ 250 260 270 280 290 300 LCGSLLDSDT SSITMQILLK LSQHSPSLLV DHFEQLRLAA KTNPGTIPLC AQIMTTAGIG 310 320 330 340 350 360 SKETAQQALD FVLEHLPTQA LLQQEATRLC SAYPVLFTDK VLACVRQKNA ALSSQQDVGN 370 380 390 400 410 420 GLANKTSGGV TIVSLNSSSP SPPLKPVPVG APAINTTIVQ PASVVSATPT PPPSSASAPI 430 440 450 460 470 480 ASSTPVSAAT PTPQHAGYTR RVKLGDSRST GRLHPASNTH RSVTRLNVAS GSVGGLHKSM 490 500 510 520 530 540 TRLSNSQINQ QPGGSSNGNV VVQSGATPKT PTGLSNPPVT PVPPLSNNVV ITGHNRHGIP 550 560 570 580 590 600 VTSGGVTVTT SPSKVRPHSQ GPSTLLNSST VLMKYSTDAL NQSVGSISIP QSAAVATLPS 610 620 630 640 650 660 TQTQNAVSVH HASPALPQSA SNGHAKSVMK LPVNGNSEVI VSGPTTNVAP RRSDNTSRTL 670 680 690 700 710 720 LNANSVMDQR MSTFEPYQIM RDPVQQFCEK NFNSIKSYMD EVSQHLPPPT RCSIEERRTK 730 740 750 760 770 780 KVAKLHFACQ IRGPHCLYSK TCFTMRTRNP KTWIHMMFLD FQVRHFVKEK CVLSTRESGI 790 800 810 820 830 840 SNLKNIWQIL KCENRSFTEL VTSQFPQVKD REILVNELRH SGFLDVFEVS KTDKSNPNCN 850 860 870 880 890 900 ELEYQWGCFL CNHPDKAVGF LNGSNQPMIE GQLKEKKGKW RLFRRWRTRY FTLSGAHLSC 910 920 930 940 950 960 KGSSGGESID VNQIRSVKVS RGARNIPKAF EIFTADQTLI LKPKDGKNAE EWVQCLSIVV 970 980 AHSQARDNPT AKTNSLPARS IGSSKPSF
	Isoform C. Checksum: BE3A08490E2855E9	FASTA	994	108,105
	10 20 30 40 50 60 MHELFNKVLA KRDLSRAGDL FSVPDADIVD DITEVLSEIS PIISHADYVK NNNDQSVVEI 70 80 90 100 110 120 CVTRVLSCIR ETKTAERYCA ALVDLLRTCL LWNLQPSGTT KEEPPHAKIA ADIISSIFLN 130 140 150 160 170 180 YDKKELMKIA LPVAVQFLPK GNRELSRNLA SYLSLAAIDH AGLLSPHTES VMESILSGNY 190 200 210 220 230 240 GLLRVLSQVY EVAPEAVTPH APLLMPLLPQ CDPQERMAVF QLYLLIVQKS PEVLESCVPQ 250 260 270 280 290 300 LCGSLLDSDT SSITMQILLK LSQHSPSLLV DHFEQLRLAA KTNPGTIPLC AQIMTTAGIG 310 320 330 340 350 360 SKETAQQALD FVLEHLPTQA LLQQEATRLC SAYPVLFTDK VLACVRQKNA ALSSQQDVGN 370 380 390 400 410 420 GLANKTSGGV TIVSLNSSSP SPPLKPVPVG APAINTTIVQ PASVVSATPT PPPSSASAPI 430 440 450 460 470 480 ASSTPVSAAT PTPQHAGYTR RVKLGDSRST GRLHPASNTH RSVTRLNVAS GSVGGLHKSM 490 500 510 520 530 540 TRLSNSQINQ QPGGSSNGNV VVQSGATPKT PTGLSNPPVT PVPPLSNNVV ITGHNRHGIP 550 560 570 580 590 600 VTSGGVTVTT SPSKVRPHSQ GPSTLLNSST VLMKYSTDAL NQSVGSISIP QSAAVATLPS 610 620 630 640 650 660 TQTQNAVSVH HASPALPQSA SNGHAKSVMK LPVNGNSEVI VSGPTTNVAP RRSDNTSRTL 670 680 690 700 710 720 LNANSVMDQR MSTFEPYQIM RDPVQQFCEK NFNSIKSYMD EVSQHLPPPT RCSIEVSNEF 730 740 750 760 770 780 AERRTKKVAK LHFACQIRGP HCLYSKTCFT MRTRNPKTWI HMMFLDFQVR HFVKEKCVLS 790 800 810 820 830 840 TRESGISNLK NIWQILKCEN RSFTELVTSQ FPQVKDREIL VNELRHSGFL DVFEVSKTDK 850 860 870 880 890 900 SNPNCNELEY QWGCFLCNHP DKAVGFLNGS NQPMIEGQLK EKKGKWRLFR RWRTRYFTLS 910 920 930 940 950 960 GAHLSCKGSS GGESIDVNQI RSVKVSRGAR NIPKAFEIFT ADQTLILKPK DGKNAEEWVQ 970 980 990 CLSIVVAHSQ ARDNPTAKTN SLPARSIGSS KPSF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING (ISOFORMS A AND B).
[3]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Strain: Berkeley.
[4]	"Mutations affecting the development of the peripheral nervous system in Drosophila: a molecular screen for novel proteins." Prokopenko S.N., He Y., Lu Y., Bellen H.J. Genetics 156:1691-1715(2000) [PubMed: 11102367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-715 (ISOFORM C), FUNCTION.
[5]	"The growth regulators warts/lats and melted interact in a bistable loop to specify opposite fates in Drosophila R8 photoreceptors." Mikeladze-Dvali T., Wernet M.F., Pistillo D., Mazzoni E.O., Teleman A.A., Chen Y.-W., Cohen S., Desplan C. Cell 122:775-787(2005) [PubMed: 16143107] [Abstract] Cited for: FUNCTION.
[6]	"Drosophila Melted modulates FOXO and TOR activity." Teleman A.A., Chen Y.-W., Cohen S.M. Dev. Cell 9:271-281(2005) [PubMed: 16054033] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TSC1 AND FOXO.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014296 Genomic DNA. Translation: AAF50606.1. AE014296 Genomic DNA. Translation: AAX52758.1. BT029129 mRNA. Translation: ABJ17062.1. AF205831 mRNA. Translation: AAF14808.1.
RefSeq	NP_001014572.1. NP_523953.1.
UniGene	Dm.9528
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9VS24.
Genome annotation databases
Ensembl	FBgn0023001. Drosophila melanogaster. [Contig view]
GeneID	38785.
KEGG	dme:Dmel_CG8624.
NMPDR	fig|7227.3.peg.8624.
Organism-specific databases
FlyBase	FBgn0023001. melt.
Phylogenomic databases
HOGENOM	Q9VS24.
OMA	Q9VS24. GFSETAG.
Gene expression databases
ArrayExpress	Q9VS24.
Family and domain databases
InterPro	IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF00169. PH. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	810368.

Entry information

Entry name

MELT_DROME

Accession

Primary (citable) accession number: Q9VS24
Secondary accession number(s): Q058T9, Q59E21, Q9U422

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project