Reviewed, UniProtKB/Swiss-Prot Q9VRX6 (INX4_DROME)

Names and origin

Protein names

Recommended name:
    Innexin inx4
      Short name=Innexin-4
Alternative name(s):
    Protein zero population growth

Gene names

Name:	zpg
Synonyms:	inx4
ORF Names:	CG10125

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Structural component of the gap junctions in germline cells. Required for differentiation and survival of germline cysts in females and of spermatogonia in males; gap junctional communication between spermatogonia and somatic cyst cells may be required for normal differentiation and survival of spermatogonia. Ref.1 Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction. Note: Concentrated at the interface between germline and somatic support cells in spermatogonia, early spermatocytes and germ cells in the ovary. Ref.2
Tissue specificity	Expressed in nurse cells and oocyte during oogenesis. Uniform expression in imaginal wing disk and low expression in developing imaginal CNS. Expressed in embryonic pole cells and primordial germ cells. Ref.1 Ref.2
Developmental stage	Expressed both maternally and zygotically. First seen at the embryonic syncitial blastoderm stage. Ref.2
Sequence similarities	Belongs to the pannexin family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	germ cell development Ref.2 Inferred from mutant phenotype. Source: FlyBase
Cellular component	gap junction Ref.2 Inferred from direct assay. Source: UniProtKB integral to membrane Ref.2 Non-traceable author statement. Source: UniProtKB
Molecular function	gap junction channel activity Non-traceable author statement. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Innexin inx4		PRO_0000208499
Regions
	Topological domain	1 – 21	21	Cytoplasmic Potential
	Transmembrane	22 – 42	21	Potential
	Topological domain	43 – 110	68	Extracellular Potential
	Transmembrane	111 – 131	21	Potential
	Topological domain	132 – 186	55	Cytoplasmic Potential
	Transmembrane	187 – 207	21	Potential
	Topological domain	208 – 268	61	Extracellular Potential
	Transmembrane	269 – 289	21	Potential
	Topological domain	290 – 367	78	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9VRX6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B984A4035DA7CF3E	FASTA	367	42,784
	10 20 30 40 50 60 MYAAVKPLSK YLQFKSVHIY DAIFTLHSKV TVALLLACTF LLSSKQYFGD PIQCFGDKDM 70 80 90 100 110 120 DYVHAFCWIY GAYVSDNVTV TPLRNGAAQC RPDAVSKVVP PENRNYITYY QWVVLVLLLE 130 140 150 160 170 180 SFVFYMPAFL WKIWEGGRLK HLCDDFHKMA VCKDKSRTHL RVLVNYFSSD YKETHFRYFV 190 200 210 220 230 240 SYVFCEILNL SISILNFLLL DVFFGGFWGR YRNALLSLYN GDYNQWNIIT MAVFPKCAKC 250 260 270 280 290 300 EMYKGGPSGS SNIYDYLCLL PLNILNEKIF AFLWIWFILV AMLISLKFLY RLATVLYPGM 310 320 330 340 350 360 RLQLLRARAR FMPKKHLQVA LRNCSFGDWF VLMRVGNNIS PELFRKLLEE LYEAQSLIKI PPGADKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gap junctions in Drosophila: developmental expression of the entire innexin gene family." Stebbings L.A., Todman M.G., Phillips R., Greer C.E., Tam J., Phelan P., Jacobs K., Bacon J.P., Davies J.A. Mech. Dev. 113:197-205(2002) [PubMed: 11960713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, TISSUE SPECIFICITY. Tissue: Ovary.
[2]	"A germline-specific gap junction protein required for survival of differentiating early germ cells." Tazuke S.I., Schulz C., Gilboa L., Fogarty M., Mahowald A.P., Guichet A., Ephrussi A., Wood C.G., Lehmann R., Fuller M.T. Development 129:2529-2539(2002) [PubMed: 11973283] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Ovary.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF271718 mRNA. Translation: AAL36976.1. AE014296 Genomic DNA. Translation: AAF50655.1. AY094856 mRNA. Translation: AAM11209.1.
RefSeq	NP_648049.1.
UniGene	Dm.3496
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:17426N.
IntAct	Q9VRX6. 1 interaction.
Proteomic databases
PRIDE	Q9VRX6.
Genome annotation databases
Ensembl	FBgn0024177. Drosophila melanogaster. [Contig view]
GeneID	251414.
KEGG	dme:Dmel_CG10125.
NMPDR	fig|7227.3.peg.8556.
Organism-specific databases
FlyBase	FBgn0024177. zpg.
Phylogenomic databases
HOGENOM	Q9VRX6.
OMA	Q9VRX6. WITFNSA.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-015465-MON.
Gene expression databases
ArrayExpress	Q9VRX6.
GermOnline	CG10125. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000990. Innexin. [Graphical view]
PANTHER	PTHR11893. Innexin. 1 hit.
Pfam	PF00876. Innexin. 1 hit. [Graphical view]
PRINTS	PR01262. INNEXIN.
PROSITE	PS51013. PANNEXIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	843533.

Entry information

Entry name

INX4_DROME

Accession

Primary (citable) accession number: Q9VRX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project