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Protein

E3 ubiquitin-protein ligase Bre1

Gene

Bre1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-117' of histone H2B. H2B 'Lys-117' ubiquitination gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It thereby plays a central role in histone code and gene regulation. Required for the expression of Notch target genes in development by affecting the levels of Su(H) in imaginal disk cells and stimulating the Su(H)-mediated transcription of Notch-specific genes.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri991 – 103040RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB
  • ubiquitin-protein transferase activity Source: FlyBase
  • zinc ion binding Source: FlyBase

GO - Biological processi

  • histone modification Source: FlyBase
  • histone ubiquitination Source: UniProtKB
  • Notch signaling pathway Source: UniProtKB-KW
  • peripheral nervous system development Source: FlyBase
  • phagocytosis Source: FlyBase
  • protein monoubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Ligase

Keywords - Biological processi

Notch signaling pathway, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Bre1 (EC:6.3.2.-)
Alternative name(s):
dBre1
Gene namesi
Name:Bre1
ORF Names:CG10542
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0086694. Bre1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044E3 ubiquitin-protein ligase Bre1PRO_0000055846Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei242 – 2421Phosphothreonine1 Publication
Modified residuei243 – 2431Phosphoserine1 Publication
Modified residuei632 – 6321Phosphoserine1 Publication
Modified residuei638 – 6381Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VRP9.
PRIDEiQ9VRP9.

PTM databases

iPTMnetiQ9VRP9.

Expressioni

Gene expression databases

BgeeiQ9VRP9.
ExpressionAtlasiQ9VRP9. differential.
GenevisibleiQ9VRP9. DM.

Interactioni

Protein-protein interaction databases

BioGridi64112. 4 interactions.
IntActiQ9VRP9. 7 interactions.
MINTiMINT-767697.
STRINGi7227.FBpp0076822.

Structurei

3D structure databases

ProteinModelPortaliQ9VRP9.
SMRiQ9VRP9. Positions 982-1042.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili43 – 8139Sequence analysisAdd
BLAST
Coiled coili218 – 386169Sequence analysisAdd
BLAST
Coiled coili417 – 546130Sequence analysisAdd
BLAST
Coiled coili664 – 767104Sequence analysisAdd
BLAST
Coiled coili794 – 970177Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the BRE1 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri991 – 103040RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0978. Eukaryota.
ENOG410Y5C6. LUCA.
InParanoidiQ9VRP9.
KOiK10696.
OMAiPNLGHPE.
OrthoDBiEOG7DVD99.
PhylomeDBiQ9VRP9.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VRP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRSADDAT GSSCLVAAAA AGQPPIKKVH FEPHLIGPVS TLEEMDIKVL
60 70 80 90 100
EFQNKKLAQR IEQRMRTEAE LRHRIEQLEK RQTQDDAVLN VVNRYWNQLN
110 120 130 140 150
EDIRVLLQRF DAETADELEN RNENEVTTSF LAQLSTWDKE ELDEKLANRV
160 170 180 190 200
QVSKRAVAKI VQVIDRLMQR NEKITHVLKG DSLASAGSGS GAGAGGEEEQ
210 220 230 240 250
QQASGDAETT TSSAGVHALE ETLKQTHIEI MSENHKLQNL NTSLHEKFHT
260 270 280 290 300
MSLKMKEYQD AHTAKETENA ELKNQIDELQ YDLEKIHCRN DKLENHLAEA
310 320 330 340 350
IEKLKAYHQI YGDPNKSTNS AKTPTTTGSG GATTSVNSQL LEELQKELEE
360 370 380 390 400
YRELANNRLQ ELDKLHATHR ETLKEVEKLK MDIRQLPESV IVETTEYKCL
410 420 430 440 450
QSQFSVLYNE SMQIKTMLDE TRNQLQTSKN QHLRQIEVME SEELIAQKKV
460 470 480 490 500
RSEMIQMEDV LALIRKEYET LRIEFEQNMA ANEQTAPINR EMRHLITSLQ
510 520 530 540 550
NHNGQLKGEV QRYKRKYKDT STDNLKLRQE LADALATLEG NKLQAATGAA
560 570 580 590 600
GEEIKQENST GVKEENSNNV SASGQTNQTN SGNDTNVAIK EENHISAEDE
610 620 630 640 650
ADDEASGKDV KDGIKQEKLS SGDAAAAEKK DSPGPGNSTS SATNSVPVKN
660 670 680 690 700
EKDSKDGVKG KDVKAVESET VRDLKAQLKK ALNDQKEMKL LLDMYKGVSK
710 720 730 740 750
DQRDKVQLMA TEKKLRSEIE ELRQQLKKLQ ESKREERKKL ADEEALRKIK
760 770 780 790 800
QLEEQKYELQ KQMANHKPTD NSWGSGAPGT ANYTRPFVGS HEEEALLNEM
810 820 830 840 850
EVTGQAFEDM QEQNSRLIQQ LREKDDANFK LMSERIKANQ LHKLLREEKT
860 870 880 890 900
VLEDQMATAT TQIEAMHIVL RKLEEKERSL QATVASIEKE LMLRQQAMEM
910 920 930 940 950
HKRKAIESAQ SAADLKLHLE KYHAQMKEAQ QVVAEKTSSL EAEAYKTKRL
960 970 980 990 1000
QEELAQFKRK AERMKKMEMS GTTIDEVMIE EIREYKETLT CPSCKVKRKD
1010 1020 1030 1040
AVLSKCFHVF CYDCLRTRYE TRQRKCPKCN CAFGANDYHR LYLQ
Length:1,044
Mass (Da):119,060
Last modified:October 1, 2002 - v2
Checksum:i426B02E6C90B37BC
GO

Sequence cautioni

The sequence AAL13880.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171A → V in AAN71372 (PubMed:12537569).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50744.2.
AY058651 mRNA. Translation: AAL13880.1. Different initiation.
BT001617 mRNA. Translation: AAN71372.1.
BT082047 mRNA. Translation: ACO95725.1.
RefSeqiNP_001286950.1. NM_001300021.1.
NP_647989.2. NM_139732.3.
UniGeneiDm.855.

Genome annotation databases

EnsemblMetazoaiFBtr0077116; FBpp0076822; FBgn0086694.
FBtr0344538; FBpp0310889; FBgn0086694.
GeneIDi38652.
KEGGidme:Dmel_CG10542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50744.2.
AY058651 mRNA. Translation: AAL13880.1. Different initiation.
BT001617 mRNA. Translation: AAN71372.1.
BT082047 mRNA. Translation: ACO95725.1.
RefSeqiNP_001286950.1. NM_001300021.1.
NP_647989.2. NM_139732.3.
UniGeneiDm.855.

3D structure databases

ProteinModelPortaliQ9VRP9.
SMRiQ9VRP9. Positions 982-1042.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64112. 4 interactions.
IntActiQ9VRP9. 7 interactions.
MINTiMINT-767697.
STRINGi7227.FBpp0076822.

PTM databases

iPTMnetiQ9VRP9.

Proteomic databases

PaxDbiQ9VRP9.
PRIDEiQ9VRP9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077116; FBpp0076822; FBgn0086694.
FBtr0344538; FBpp0310889; FBgn0086694.
GeneIDi38652.
KEGGidme:Dmel_CG10542.

Organism-specific databases

CTDi38652.
FlyBaseiFBgn0086694. Bre1.

Phylogenomic databases

eggNOGiKOG0978. Eukaryota.
ENOG410Y5C6. LUCA.
InParanoidiQ9VRP9.
KOiK10696.
OMAiPNLGHPE.
OrthoDBiEOG7DVD99.
PhylomeDBiQ9VRP9.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

GenomeRNAii38652.
PROiQ9VRP9.

Gene expression databases

BgeeiQ9VRP9.
ExpressionAtlasiQ9VRP9. differential.
GenevisibleiQ9VRP9. DM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley1 Publication.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BerkeleyImported.
    Tissue: Embryo1 Publication.
  4. Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
  5. "Bre1 is required for Notch signaling and histone modification."
    Bray S., Musisi H., Bienz M.
    Dev. Cell 8:279-286(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-242; SER-243; SER-632 AND SER-638, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiBRE1_DROME
AccessioniPrimary (citable) accession number: Q9VRP9
Secondary accession number(s): C3KGI2, Q8IGT1, Q95TN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.