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Protein

Ubiquitin carboxyl-terminal hydrolase 36

Gene

scny

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4).1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei181 – 1811Nucleophile
Active sitei439 – 4391Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  1. germ-line stem cell maintenance Source: UniProtKB
  2. histone H2B conserved C-terminal lysine deubiquitination Source: FlyBase
  3. negative regulation of defense response to bacterium Source: FlyBase
  4. negative regulation of innate immune response Source: FlyBase
  5. positive regulation of chromatin silencing Source: FlyBase
  6. positive regulation of stem cell maintenance Source: FlyBase
  7. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  8. protein deubiquitination Source: UniProtKB
  9. regulation of apoptotic process Source: FlyBase
  10. regulation of proteasomal protein catabolic process Source: GO_Central
  11. somatic stem cell maintenance Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 36
Protein scrawny
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36
Gene namesi
Name:scny
Synonyms:Usp36
ORF Names:CG5505
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0260936. scny.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. nucleolus Source: UniProtKB
  2. nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi181 – 1811C → S: Loss of H2B deubiquitination. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10381038Ubiquitin carboxyl-terminal hydrolase 36PRO_0000378498Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei513 – 5131Phosphoserine1 Publication
Modified residuei515 – 5151Phosphoserine1 Publication
Modified residuei658 – 6581Phosphothreonine1 Publication
Modified residuei662 – 6621Phosphothreonine1 Publication
Modified residuei672 – 6721Phosphoserine1 Publication
Modified residuei674 – 6741Phosphoserine1 Publication
Modified residuei747 – 7471Phosphoserine1 Publication
Modified residuei779 – 7791Phosphoserine1 Publication
Modified residuei782 – 7821Phosphothreonine1 Publication
Modified residuei785 – 7851Phosphoserine1 Publication
Modified residuei819 – 8191Phosphoserine1 Publication
Modified residuei825 – 8251Phosphothreonine1 Publication
Modified residuei843 – 8431Phosphoserine1 Publication
Modified residuei846 – 8461Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VRP5.

Expressioni

Gene expression databases

BgeeiQ9VRP5.
ExpressionAtlasiQ9VRP5. differential.

Interactioni

Subunit structurei

Interacts with atms/PAF1, but not with CycT.1 Publication

Protein-protein interaction databases

IntActiQ9VRP5. 96 interactions.
STRINGi7227.FBpp0076805.

Structurei

3D structure databases

ProteinModelPortaliQ9VRP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 480309USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121889.
InParanoidiQ9VRP5.
KOiK11855.
OMAiNEMDRGR.
OrthoDBiEOG7XDBF6.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform D (identifier: Q9VRP5-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVSMAVCET ANVVNAALRE SLGGNSSAGS STDQAKSGED TNGSLQNHIV
60 70 80 90 100
ANAKRILMAK IEYEEVPNYH ESVLENLKSK YIVIKPGNPG AINGFSGKNN
110 120 130 140 150
TGKLVGANGH DNNGARKQAE HPNNQSHHIN HHNHQHPTSN PNELPKPKRV
160 170 180 190 200
LYPRENIRIG WKQSERKWQV GTGMINVGNT CYLNSTLQAL LHIPALANWL
210 220 230 240 250
VSEQAHLADC NVAEPGSGCI ICAMTKTLLA TQSNQSAVRP FLIYSKLKQI
260 270 280 290 300
CKHMVVGRQE DAHEFLRFLV EAMERAYLMR FRNYKELDQL VKETTPLGQI
310 320 330 340 350
FGGYLRSEVR CLSCNHVSIT FQHFQDLLLD IRKADSLEDA FEGHFSRERL
360 370 380 390 400
EDMGYKCEGC KKKVSATKQF SLERAPITLC IQLKRFSMIG NKLTKQISFK
410 420 430 440 450
SRIDLSKYAA RSQAAQAQPL TYRLVSMVTH LGASQHCGHY TAIGSTDTGS
460 470 480 490 500
FYNFDDSYVR PIAMHSVCNT NAYIMFFELD LSQAASPAAN RPNGVRLTNG
510 520 530 540 550
HSTTPVPAAT VSSPSPTRFI GPQLPAGGAN GYTNGNAQKT AIQFKQQNQQ
560 570 580 590 600
SPQNGLQLGT GKFQDTAKPP LVGAHAKGEA TSAPTANGNK SSSPSSNSSS
610 620 630 640 650
NHKSINQQQY LPISSDDEDI EDEMKPRPTT AQLPSMPNMT ENHTEPKAKS
660 670 680 690 700
PVKIQVKTPV KTPLKSLVPY ESASEEEEAP LPNPRKRPSG EDSSESDQES
710 720 730 740 750
GQTNGHSKTN GSHTNGSASS SVHVNNSKQK TDAIDEIFKS LKKSADSDED
760 770 780 790 800
DDEEEPSIQL TNGWHPQKQS QSQSKAPPSP KTPPSPAVIK SKTGIWKVTR
810 820 830 840 850
NDEVDAIEDD VDVVVVEGSP VKIPTPNKNH RNPFSSSKPS TDSPATPGAK
860 870 880 890 900
RQKLLNGSAL KSHQQPRVGN GYQSNATSNG STINELLKQS YRGYGSPVLS
910 920 930 940 950
WNGKPAELEK ELLVDAREQR QRDIDDDEEN EMDRGRQRKV KSGSAKGNNA
960 970 980 990 1000
SNSTPGYNPF QEYEGQKRWN KNGGGGGFPR FYNQNYRQNF QQRNKFKFNR
1010 1020 1030
FGGPGSAKFQ QQRALQRHLS AGGGFSRRQP SAQQQQQT

Note: No experimental confirmation available.

Length:1,038
Mass (Da):114,088
Last modified:April 16, 2014 - v3
Checksum:i98CECD6993333E22
GO
Isoform B (identifier: Q9VRP5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     71-110: ESVLENLKSK...TGKLVGANGH → MTVIMVDGFA...KLSTVLFTFA

Note: No experimental confirmation available.

Show »
Length:968
Mass (Da):107,252
Checksum:i941F6973D4435C8A
GO
Isoform C (identifier: Q9VRP5-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     91-110: AINGFSGKNNTGKLVGANGH → MLHSPVPRYNKCVPFPTLRT

Note: No experimental confirmation available.

Show »
Length:948
Mass (Da):104,961
Checksum:i29E5A05BB8761243
GO
Isoform G (identifier: Q9VRP5-7) [UniParc]FASTAAdd to basket

Also known as: H

The sequence of this isoform differs from the canonical sequence as follows:
     911-1038: ELLVDAREQR...QPSAQQQQQT → ETFELVCAKR...PDPDRLTDGR

Note: No experimental confirmation available.

Show »
Length:976
Mass (Da):105,957
Checksum:i89E02FAC5B5FCC82
GO
Isoform I (identifier: Q9VRP5-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     91-110: AINGFSGKNNTGKLVGANGH → MLHSPVPRYNKCVPFPTLRT
     911-1038: ELLVDAREQR...QPSAQQQQQT → ETFELVCAKR...PDPDRLTDGR

Note: No experimental confirmation available.

Show »
Length:886
Mass (Da):96,830
Checksum:i572BE9A8370C7615
GO
Isoform J (identifier: Q9VRP5-9) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     71-110: ESVLENLKSK...TGKLVGANGH → MTVIMVDGFA...KLSTVLFTFA
     911-1038: ELLVDAREQR...QPSAQQQQQT → ETFELVCAKR...PDPDRLTDGR

Note: No experimental confirmation available.

Show »
Length:906
Mass (Da):99,121
Checksum:i74BBC44F1AB468F4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151A → T in AAQ22589 (Ref. 4) Curated
Sequence conflicti642 – 6421N → D in AAQ22589 (Ref. 4) Curated
Sequence conflicti642 – 6421N → D in AAO42671 (Ref. 4) Curated
Sequence conflicti766 – 7661P → T in AAQ22589 (Ref. 4) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9090Missing in isoform C and isoform I. 1 PublicationVSP_037590Add
BLAST
Alternative sequencei1 – 7070Missing in isoform B and isoform J. 1 PublicationVSP_037591Add
BLAST
Alternative sequencei71 – 11040ESVLE…GANGH → MTVIMVDGFALWLLYKLFLS PCCLLLWHVLKLSTVLFTFA in isoform B and isoform J. 1 PublicationVSP_037592Add
BLAST
Alternative sequencei91 – 11020AINGF…GANGH → MLHSPVPRYNKCVPFPTLRT in isoform C and isoform I. 1 PublicationVSP_037593Add
BLAST
Alternative sequencei911 – 1038128ELLVD…QQQQT → ETFELVCAKRIAGHGSVEGS DIVEGSVAVDAAVTSGSDSK DVVVIAVAVTDTTADAPDPD RLTDGR in isoform G, isoform I and isoform J. CuratedVSP_054032Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAN12107.1.
AE014296 Genomic DNA. Translation: AAN12108.2.
AE014296 Genomic DNA. Translation: AAN12109.1.
AE014296 Genomic DNA. Translation: AAN12110.2.
AE014296 Genomic DNA. Translation: AAN12111.1.
AE014296 Genomic DNA. Translation: AFH04310.1.
AE014296 Genomic DNA. Translation: AFH04311.1.
AY051916 mRNA. Translation: AAK93340.1.
BT004507 mRNA. Translation: AAO42671.1.
BT010120 mRNA. Translation: AAQ22589.1.
BT046169 mRNA. Translation: ACI47091.1.
RefSeqiNP_001246639.1. NM_001259710.2. [Q9VRP5-7]
NP_001246640.1. NM_001259711.2. [Q9VRP5-7]
NP_647986.3. NM_139729.3. [Q9VRP5-9]
NP_729092.1. NM_168132.3. [Q9VRP5-3]
NP_729093.2. NM_168133.2. [Q9VRP5-8]
NP_729094.1. NM_168134.2. [Q9VRP5-6]
NP_729095.1. NM_168135.2. [Q9VRP5-2]
UniGeneiDm.6825.

Genome annotation databases

EnsemblMetazoaiFBtr0077100; FBpp0076806; FBgn0260936. [Q9VRP5-3]
GeneIDi38648.
KEGGidme:Dmel_CG5505.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAN12107.1.
AE014296 Genomic DNA. Translation: AAN12108.2.
AE014296 Genomic DNA. Translation: AAN12109.1.
AE014296 Genomic DNA. Translation: AAN12110.2.
AE014296 Genomic DNA. Translation: AAN12111.1.
AE014296 Genomic DNA. Translation: AFH04310.1.
AE014296 Genomic DNA. Translation: AFH04311.1.
AY051916 mRNA. Translation: AAK93340.1.
BT004507 mRNA. Translation: AAO42671.1.
BT010120 mRNA. Translation: AAQ22589.1.
BT046169 mRNA. Translation: ACI47091.1.
RefSeqiNP_001246639.1. NM_001259710.2. [Q9VRP5-7]
NP_001246640.1. NM_001259711.2. [Q9VRP5-7]
NP_647986.3. NM_139729.3. [Q9VRP5-9]
NP_729092.1. NM_168132.3. [Q9VRP5-3]
NP_729093.2. NM_168133.2. [Q9VRP5-8]
NP_729094.1. NM_168134.2. [Q9VRP5-6]
NP_729095.1. NM_168135.2. [Q9VRP5-2]
UniGeneiDm.6825.

3D structure databases

ProteinModelPortaliQ9VRP5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9VRP5. 96 interactions.
STRINGi7227.FBpp0076805.

Proteomic databases

PaxDbiQ9VRP5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077100; FBpp0076806; FBgn0260936. [Q9VRP5-3]
GeneIDi38648.
KEGGidme:Dmel_CG5505.

Organism-specific databases

CTDi38648.
FlyBaseiFBgn0260936. scny.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121889.
InParanoidiQ9VRP5.
KOiK11855.
OMAiNEMDRGR.
OrthoDBiEOG7XDBF6.

Miscellaneous databases

GenomeRNAii38648.
NextBioi809699.

Gene expression databases

BgeeiQ9VRP5.
ExpressionAtlasiQ9VRP5. differential.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Strain: Berkeley.
    Tissue: Embryo.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS C AND D).
    Strain: Berkeley.
    Tissue: Testis.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-513; SER-515; THR-658; THR-662; SER-672; SER-674; SER-747; SER-779; THR-782; SER-785; SER-819; THR-825; SER-843 AND THR-846, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.
  6. "Drosophila stem cells share a common requirement for the histone H2B ubiquitin protease scrawny."
    Buszczak M., Paterno S., Spradling A.C.
    Science 323:248-251(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATMS, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-181.

Entry informationi

Entry nameiUBP36_DROME
AccessioniPrimary (citable) accession number: Q9VRP5
Secondary accession number(s): M9NE30
, Q7YTX7, Q86NM9, Q8IQ57, Q8IQ58, Q8IQ59, Q8IQ60, Q960Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 16, 2014
Last modified: March 4, 2015
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.