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Q9VRP5

- UBP36_DROME

UniProt

Q9VRP5 - UBP36_DROME

Protein

Ubiquitin carboxyl-terminal hydrolase 36

Gene

scny

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 3 (16 Apr 2014)
      Previous versions | rss
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    Functioni

    Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4).1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei181 – 1811Nucleophile
    Active sitei439 – 4391Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. ubiquitin-specific protease activity Source: FlyBase
    3. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. germ-line stem cell maintenance Source: UniProtKB
    2. histone H2B conserved C-terminal lysine deubiquitination Source: FlyBase
    3. negative regulation of innate immune response Source: FlyBase
    4. positive regulation of chromatin silencing Source: FlyBase
    5. positive regulation of stem cell maintenance Source: FlyBase
    6. protein deubiquitination Source: UniProtKB
    7. regulation of apoptotic process Source: FlyBase
    8. somatic stem cell maintenance Source: UniProtKB
    9. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.097.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 36
    Protein scrawny
    Ubiquitin thioesterase 36
    Ubiquitin-specific-processing protease 36
    Gene namesi
    Name:scny
    Synonyms:Usp36
    ORF Names:CG5505
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0260936. scny.

    Subcellular locationi

    Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB
    2. nucleus Source: FlyBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi181 – 1811C → S: Loss of H2B deubiquitination. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10381038Ubiquitin carboxyl-terminal hydrolase 36PRO_0000378498Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei513 – 5131Phosphoserine1 Publication
    Modified residuei515 – 5151Phosphoserine1 Publication
    Modified residuei658 – 6581Phosphothreonine1 Publication
    Modified residuei662 – 6621Phosphothreonine1 Publication
    Modified residuei672 – 6721Phosphoserine1 Publication
    Modified residuei674 – 6741Phosphoserine1 Publication
    Modified residuei747 – 7471Phosphoserine1 Publication
    Modified residuei779 – 7791Phosphoserine1 Publication
    Modified residuei782 – 7821Phosphothreonine1 Publication
    Modified residuei785 – 7851Phosphoserine1 Publication
    Modified residuei819 – 8191Phosphoserine1 Publication
    Modified residuei825 – 8251Phosphothreonine1 Publication
    Modified residuei843 – 8431Phosphoserine1 Publication
    Modified residuei846 – 8461Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9VRP5.

    Expressioni

    Gene expression databases

    BgeeiQ9VRP5.

    Interactioni

    Subunit structurei

    Interacts with atms/PAF1, but not with CycT.1 Publication

    Protein-protein interaction databases

    STRINGi7227.FBpp0076805.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VRP5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini172 – 480309USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    GeneTreeiENSGT00750000117288.
    InParanoidiQ86NM9.
    KOiK11855.
    OMAiENEMDRG.
    OrthoDBiEOG7XDBF6.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform D (identifier: Q9VRP5-3) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPVSMAVCET ANVVNAALRE SLGGNSSAGS STDQAKSGED TNGSLQNHIV     50
    ANAKRILMAK IEYEEVPNYH ESVLENLKSK YIVIKPGNPG AINGFSGKNN 100
    TGKLVGANGH DNNGARKQAE HPNNQSHHIN HHNHQHPTSN PNELPKPKRV 150
    LYPRENIRIG WKQSERKWQV GTGMINVGNT CYLNSTLQAL LHIPALANWL 200
    VSEQAHLADC NVAEPGSGCI ICAMTKTLLA TQSNQSAVRP FLIYSKLKQI 250
    CKHMVVGRQE DAHEFLRFLV EAMERAYLMR FRNYKELDQL VKETTPLGQI 300
    FGGYLRSEVR CLSCNHVSIT FQHFQDLLLD IRKADSLEDA FEGHFSRERL 350
    EDMGYKCEGC KKKVSATKQF SLERAPITLC IQLKRFSMIG NKLTKQISFK 400
    SRIDLSKYAA RSQAAQAQPL TYRLVSMVTH LGASQHCGHY TAIGSTDTGS 450
    FYNFDDSYVR PIAMHSVCNT NAYIMFFELD LSQAASPAAN RPNGVRLTNG 500
    HSTTPVPAAT VSSPSPTRFI GPQLPAGGAN GYTNGNAQKT AIQFKQQNQQ 550
    SPQNGLQLGT GKFQDTAKPP LVGAHAKGEA TSAPTANGNK SSSPSSNSSS 600
    NHKSINQQQY LPISSDDEDI EDEMKPRPTT AQLPSMPNMT ENHTEPKAKS 650
    PVKIQVKTPV KTPLKSLVPY ESASEEEEAP LPNPRKRPSG EDSSESDQES 700
    GQTNGHSKTN GSHTNGSASS SVHVNNSKQK TDAIDEIFKS LKKSADSDED 750
    DDEEEPSIQL TNGWHPQKQS QSQSKAPPSP KTPPSPAVIK SKTGIWKVTR 800
    NDEVDAIEDD VDVVVVEGSP VKIPTPNKNH RNPFSSSKPS TDSPATPGAK 850
    RQKLLNGSAL KSHQQPRVGN GYQSNATSNG STINELLKQS YRGYGSPVLS 900
    WNGKPAELEK ELLVDAREQR QRDIDDDEEN EMDRGRQRKV KSGSAKGNNA 950
    SNSTPGYNPF QEYEGQKRWN KNGGGGGFPR FYNQNYRQNF QQRNKFKFNR 1000
    FGGPGSAKFQ QQRALQRHLS AGGGFSRRQP SAQQQQQT 1038

    Note: No experimental confirmation available.

    Length:1,038
    Mass (Da):114,088
    Last modified:April 16, 2014 - v3
    Checksum:i98CECD6993333E22
    GO
    Isoform B (identifier: Q9VRP5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-70: Missing.
         71-110: ESVLENLKSK...TGKLVGANGH → MTVIMVDGFA...KLSTVLFTFA

    Note: No experimental confirmation available.

    Show »
    Length:968
    Mass (Da):107,252
    Checksum:i941F6973D4435C8A
    GO
    Isoform C (identifier: Q9VRP5-6) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-90: Missing.
         91-110: AINGFSGKNNTGKLVGANGH → MLHSPVPRYNKCVPFPTLRT

    Note: No experimental confirmation available.

    Show »
    Length:948
    Mass (Da):104,961
    Checksum:i29E5A05BB8761243
    GO
    Isoform G (identifier: Q9VRP5-7) [UniParc]FASTAAdd to Basket

    Also known as: H

    The sequence of this isoform differs from the canonical sequence as follows:
         911-1038: ELLVDAREQR...QPSAQQQQQT → ETFELVCAKR...PDPDRLTDGR

    Note: No experimental confirmation available.

    Show »
    Length:976
    Mass (Da):105,957
    Checksum:i89E02FAC5B5FCC82
    GO
    Isoform I (identifier: Q9VRP5-8) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-90: Missing.
         91-110: AINGFSGKNNTGKLVGANGH → MLHSPVPRYNKCVPFPTLRT
         911-1038: ELLVDAREQR...QPSAQQQQQT → ETFELVCAKR...PDPDRLTDGR

    Note: No experimental confirmation available.

    Show »
    Length:886
    Mass (Da):96,830
    Checksum:i572BE9A8370C7615
    GO
    Isoform J (identifier: Q9VRP5-9) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-70: Missing.
         71-110: ESVLENLKSK...TGKLVGANGH → MTVIMVDGFA...KLSTVLFTFA
         911-1038: ELLVDAREQR...QPSAQQQQQT → ETFELVCAKR...PDPDRLTDGR

    Note: No experimental confirmation available.

    Show »
    Length:906
    Mass (Da):99,121
    Checksum:i74BBC44F1AB468F4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti115 – 1151A → T in AAQ22589. 1 PublicationCurated
    Sequence conflicti642 – 6421N → D in AAQ22589. 1 PublicationCurated
    Sequence conflicti642 – 6421N → D in AAO42671. 1 PublicationCurated
    Sequence conflicti766 – 7661P → T in AAQ22589. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9090Missing in isoform C and isoform I. 1 PublicationVSP_037590Add
    BLAST
    Alternative sequencei1 – 7070Missing in isoform B and isoform J. 1 PublicationVSP_037591Add
    BLAST
    Alternative sequencei71 – 11040ESVLE…GANGH → MTVIMVDGFALWLLYKLFLS PCCLLLWHVLKLSTVLFTFA in isoform B and isoform J. 1 PublicationVSP_037592Add
    BLAST
    Alternative sequencei91 – 11020AINGF…GANGH → MLHSPVPRYNKCVPFPTLRT in isoform C and isoform I. 1 PublicationVSP_037593Add
    BLAST
    Alternative sequencei911 – 1038128ELLVD…QQQQT → ETFELVCAKRIAGHGSVEGS DIVEGSVAVDAAVTSGSDSK DVVVIAVAVTDTTADAPDPD RLTDGR in isoform G, isoform I and isoform J. CuratedVSP_054032Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014296 Genomic DNA. Translation: AAN12107.1.
    AE014296 Genomic DNA. Translation: AAN12108.2.
    AE014296 Genomic DNA. Translation: AAN12109.1.
    AE014296 Genomic DNA. Translation: AAN12110.2.
    AE014296 Genomic DNA. Translation: AAN12111.1.
    AE014296 Genomic DNA. Translation: AFH04310.1.
    AE014296 Genomic DNA. Translation: AFH04311.1.
    AY051916 mRNA. Translation: AAK93340.1.
    BT004507 mRNA. Translation: AAO42671.1.
    BT010120 mRNA. Translation: AAQ22589.1.
    BT046169 mRNA. Translation: ACI47091.1.
    RefSeqiNP_001246639.1. NM_001259710.2. [Q9VRP5-7]
    NP_001246640.1. NM_001259711.2. [Q9VRP5-7]
    NP_647986.3. NM_139729.3. [Q9VRP5-9]
    NP_729092.1. NM_168132.2. [Q9VRP5-3]
    NP_729093.2. NM_168133.2. [Q9VRP5-8]
    NP_729094.1. NM_168134.2. [Q9VRP5-6]
    NP_729095.1. NM_168135.2. [Q9VRP5-2]
    UniGeneiDm.6825.

    Genome annotation databases

    EnsemblMetazoaiFBtr0307975; FBpp0300344; FBgn0260936. [Q9VRP5-7]
    FBtr0307976; FBpp0300345; FBgn0260936. [Q9VRP5-7]
    GeneIDi38648.
    KEGGidme:Dmel_CG5505.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014296 Genomic DNA. Translation: AAN12107.1 .
    AE014296 Genomic DNA. Translation: AAN12108.2 .
    AE014296 Genomic DNA. Translation: AAN12109.1 .
    AE014296 Genomic DNA. Translation: AAN12110.2 .
    AE014296 Genomic DNA. Translation: AAN12111.1 .
    AE014296 Genomic DNA. Translation: AFH04310.1 .
    AE014296 Genomic DNA. Translation: AFH04311.1 .
    AY051916 mRNA. Translation: AAK93340.1 .
    BT004507 mRNA. Translation: AAO42671.1 .
    BT010120 mRNA. Translation: AAQ22589.1 .
    BT046169 mRNA. Translation: ACI47091.1 .
    RefSeqi NP_001246639.1. NM_001259710.2. [Q9VRP5-7 ]
    NP_001246640.1. NM_001259711.2. [Q9VRP5-7 ]
    NP_647986.3. NM_139729.3. [Q9VRP5-9 ]
    NP_729092.1. NM_168132.2. [Q9VRP5-3 ]
    NP_729093.2. NM_168133.2. [Q9VRP5-8 ]
    NP_729094.1. NM_168134.2. [Q9VRP5-6 ]
    NP_729095.1. NM_168135.2. [Q9VRP5-2 ]
    UniGenei Dm.6825.

    3D structure databases

    ProteinModelPortali Q9VRP5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7227.FBpp0076805.

    Protein family/group databases

    MEROPSi C19.097.

    Proteomic databases

    PaxDbi Q9VRP5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0307975 ; FBpp0300344 ; FBgn0260936 . [Q9VRP5-7 ]
    FBtr0307976 ; FBpp0300345 ; FBgn0260936 . [Q9VRP5-7 ]
    GeneIDi 38648.
    KEGGi dme:Dmel_CG5505.

    Organism-specific databases

    CTDi 38648.
    FlyBasei FBgn0260936. scny.

    Phylogenomic databases

    eggNOGi COG5533.
    GeneTreei ENSGT00750000117288.
    InParanoidi Q86NM9.
    KOi K11855.
    OMAi ENEMDRG.
    OrthoDBi EOG7XDBF6.

    Miscellaneous databases

    GenomeRNAii 38648.
    NextBioi 809699.

    Gene expression databases

    Bgeei Q9VRP5.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
      Strain: Berkeley.
      Tissue: Embryo.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS C AND D).
      Strain: Berkeley.
      Tissue: Testis.
    5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-513; SER-515; THR-658; THR-662; SER-672; SER-674; SER-747; SER-779; THR-782; SER-785; SER-819; THR-825; SER-843 AND THR-846, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.
    6. "Drosophila stem cells share a common requirement for the histone H2B ubiquitin protease scrawny."
      Buszczak M., Paterno S., Spradling A.C.
      Science 323:248-251(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATMS, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-181.

    Entry informationi

    Entry nameiUBP36_DROME
    AccessioniPrimary (citable) accession number: Q9VRP5
    Secondary accession number(s): M9NE30
    , Q7YTX7, Q86NM9, Q8IQ57, Q8IQ58, Q8IQ59, Q8IQ60, Q960Q4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 7, 2009
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 96 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3