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Q9VRP5

- UBP36_DROME

UniProt

Q9VRP5 - UBP36_DROME

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Protein

Ubiquitin carboxyl-terminal hydrolase 36

Gene
scny, Usp36, CG5505
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4).1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei181 – 1811Nucleophile
Active sitei439 – 4391Proton acceptor By similarity

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. ubiquitin-specific protease activity Source: FlyBase
  3. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. germ-line stem cell maintenance Source: UniProtKB
  2. histone H2B conserved C-terminal lysine deubiquitination Source: FlyBase
  3. negative regulation of innate immune response Source: FlyBase
  4. positive regulation of chromatin silencing Source: FlyBase
  5. positive regulation of stem cell maintenance Source: FlyBase
  6. protein deubiquitination Source: UniProtKB
  7. regulation of apoptotic process Source: FlyBase
  8. somatic stem cell maintenance Source: UniProtKB
  9. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.097.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 36
Protein scrawny
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36
Gene namesi
Name:scny
Synonyms:Usp36
ORF Names:CG5505
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0260936. scny.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. nucleolus Source: UniProtKB
  2. nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi181 – 1811C → S: Loss of H2B deubiquitination. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10381038Ubiquitin carboxyl-terminal hydrolase 36PRO_0000378498Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei513 – 5131Phosphoserine1 Publication
Modified residuei515 – 5151Phosphoserine1 Publication
Modified residuei658 – 6581Phosphothreonine1 Publication
Modified residuei662 – 6621Phosphothreonine1 Publication
Modified residuei672 – 6721Phosphoserine1 Publication
Modified residuei674 – 6741Phosphoserine1 Publication
Modified residuei747 – 7471Phosphoserine1 Publication
Modified residuei779 – 7791Phosphoserine1 Publication
Modified residuei782 – 7821Phosphothreonine1 Publication
Modified residuei785 – 7851Phosphoserine1 Publication
Modified residuei819 – 8191Phosphoserine1 Publication
Modified residuei825 – 8251Phosphothreonine1 Publication
Modified residuei843 – 8431Phosphoserine1 Publication
Modified residuei846 – 8461Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VRP5.

Expressioni

Gene expression databases

BgeeiQ9VRP5.

Interactioni

Subunit structurei

Interacts with atms/PAF1, but not with CycT.1 Publication

Protein-protein interaction databases

STRINGi7227.FBpp0076805.

Structurei

3D structure databases

ProteinModelPortaliQ9VRP5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 480309USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00750000117288.
InParanoidiQ86NM9.
KOiK11855.
OMAiENEMDRG.
OrthoDBiEOG7XDBF6.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform D (identifier: Q9VRP5-3) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPVSMAVCET ANVVNAALRE SLGGNSSAGS STDQAKSGED TNGSLQNHIV     50
ANAKRILMAK IEYEEVPNYH ESVLENLKSK YIVIKPGNPG AINGFSGKNN 100
TGKLVGANGH DNNGARKQAE HPNNQSHHIN HHNHQHPTSN PNELPKPKRV 150
LYPRENIRIG WKQSERKWQV GTGMINVGNT CYLNSTLQAL LHIPALANWL 200
VSEQAHLADC NVAEPGSGCI ICAMTKTLLA TQSNQSAVRP FLIYSKLKQI 250
CKHMVVGRQE DAHEFLRFLV EAMERAYLMR FRNYKELDQL VKETTPLGQI 300
FGGYLRSEVR CLSCNHVSIT FQHFQDLLLD IRKADSLEDA FEGHFSRERL 350
EDMGYKCEGC KKKVSATKQF SLERAPITLC IQLKRFSMIG NKLTKQISFK 400
SRIDLSKYAA RSQAAQAQPL TYRLVSMVTH LGASQHCGHY TAIGSTDTGS 450
FYNFDDSYVR PIAMHSVCNT NAYIMFFELD LSQAASPAAN RPNGVRLTNG 500
HSTTPVPAAT VSSPSPTRFI GPQLPAGGAN GYTNGNAQKT AIQFKQQNQQ 550
SPQNGLQLGT GKFQDTAKPP LVGAHAKGEA TSAPTANGNK SSSPSSNSSS 600
NHKSINQQQY LPISSDDEDI EDEMKPRPTT AQLPSMPNMT ENHTEPKAKS 650
PVKIQVKTPV KTPLKSLVPY ESASEEEEAP LPNPRKRPSG EDSSESDQES 700
GQTNGHSKTN GSHTNGSASS SVHVNNSKQK TDAIDEIFKS LKKSADSDED 750
DDEEEPSIQL TNGWHPQKQS QSQSKAPPSP KTPPSPAVIK SKTGIWKVTR 800
NDEVDAIEDD VDVVVVEGSP VKIPTPNKNH RNPFSSSKPS TDSPATPGAK 850
RQKLLNGSAL KSHQQPRVGN GYQSNATSNG STINELLKQS YRGYGSPVLS 900
WNGKPAELEK ELLVDAREQR QRDIDDDEEN EMDRGRQRKV KSGSAKGNNA 950
SNSTPGYNPF QEYEGQKRWN KNGGGGGFPR FYNQNYRQNF QQRNKFKFNR 1000
FGGPGSAKFQ QQRALQRHLS AGGGFSRRQP SAQQQQQT 1038

Note: No experimental confirmation available.

Length:1,038
Mass (Da):114,088
Last modified:April 16, 2014 - v3
Checksum:i98CECD6993333E22
GO
Isoform B (identifier: Q9VRP5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     71-110: ESVLENLKSK...TGKLVGANGH → MTVIMVDGFA...KLSTVLFTFA

Note: No experimental confirmation available.

Show »
Length:968
Mass (Da):107,252
Checksum:i941F6973D4435C8A
GO
Isoform C (identifier: Q9VRP5-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     91-110: AINGFSGKNNTGKLVGANGH → MLHSPVPRYNKCVPFPTLRT

Note: No experimental confirmation available.

Show »
Length:948
Mass (Da):104,961
Checksum:i29E5A05BB8761243
GO
Isoform G (identifier: Q9VRP5-7) [UniParc]FASTAAdd to Basket

Also known as: H

The sequence of this isoform differs from the canonical sequence as follows:
     911-1038: ELLVDAREQR...QPSAQQQQQT → ETFELVCAKR...PDPDRLTDGR

Note: No experimental confirmation available.

Show »
Length:976
Mass (Da):105,957
Checksum:i89E02FAC5B5FCC82
GO
Isoform I (identifier: Q9VRP5-8) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     91-110: AINGFSGKNNTGKLVGANGH → MLHSPVPRYNKCVPFPTLRT
     911-1038: ELLVDAREQR...QPSAQQQQQT → ETFELVCAKR...PDPDRLTDGR

Note: No experimental confirmation available.

Show »
Length:886
Mass (Da):96,830
Checksum:i572BE9A8370C7615
GO
Isoform J (identifier: Q9VRP5-9) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     71-110: ESVLENLKSK...TGKLVGANGH → MTVIMVDGFA...KLSTVLFTFA
     911-1038: ELLVDAREQR...QPSAQQQQQT → ETFELVCAKR...PDPDRLTDGR

Note: No experimental confirmation available.

Show »
Length:906
Mass (Da):99,121
Checksum:i74BBC44F1AB468F4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9090Missing in isoform C and isoform I. VSP_037590Add
BLAST
Alternative sequencei1 – 7070Missing in isoform B and isoform J. VSP_037591Add
BLAST
Alternative sequencei71 – 11040ESVLE…GANGH → MTVIMVDGFALWLLYKLFLS PCCLLLWHVLKLSTVLFTFA in isoform B and isoform J. VSP_037592Add
BLAST
Alternative sequencei91 – 11020AINGF…GANGH → MLHSPVPRYNKCVPFPTLRT in isoform C and isoform I. VSP_037593Add
BLAST
Alternative sequencei911 – 1038128ELLVD…QQQQT → ETFELVCAKRIAGHGSVEGS DIVEGSVAVDAAVTSGSDSK DVVVIAVAVTDTTADAPDPD RLTDGR in isoform G, isoform I and isoform J. VSP_054032Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151A → T in AAQ22589. 1 Publication
Sequence conflicti642 – 6421N → D in AAQ22589. 1 Publication
Sequence conflicti642 – 6421N → D in AAO42671. 1 Publication
Sequence conflicti766 – 7661P → T in AAQ22589. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014296 Genomic DNA. Translation: AAN12107.1.
AE014296 Genomic DNA. Translation: AAN12108.2.
AE014296 Genomic DNA. Translation: AAN12109.1.
AE014296 Genomic DNA. Translation: AAN12110.2.
AE014296 Genomic DNA. Translation: AAN12111.1.
AE014296 Genomic DNA. Translation: AFH04310.1.
AE014296 Genomic DNA. Translation: AFH04311.1.
AY051916 mRNA. Translation: AAK93340.1.
BT004507 mRNA. Translation: AAO42671.1.
BT010120 mRNA. Translation: AAQ22589.1.
BT046169 mRNA. Translation: ACI47091.1.
RefSeqiNP_001246639.1. NM_001259710.2. [Q9VRP5-7]
NP_001246640.1. NM_001259711.2. [Q9VRP5-7]
NP_647986.3. NM_139729.3. [Q9VRP5-9]
NP_729092.1. NM_168132.2. [Q9VRP5-3]
NP_729093.2. NM_168133.2. [Q9VRP5-8]
NP_729094.1. NM_168134.2. [Q9VRP5-6]
NP_729095.1. NM_168135.2. [Q9VRP5-2]
UniGeneiDm.6825.

Genome annotation databases

EnsemblMetazoaiFBtr0307975; FBpp0300344; FBgn0260936. [Q9VRP5-7]
FBtr0307976; FBpp0300345; FBgn0260936. [Q9VRP5-7]
GeneIDi38648.
KEGGidme:Dmel_CG5505.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014296 Genomic DNA. Translation: AAN12107.1 .
AE014296 Genomic DNA. Translation: AAN12108.2 .
AE014296 Genomic DNA. Translation: AAN12109.1 .
AE014296 Genomic DNA. Translation: AAN12110.2 .
AE014296 Genomic DNA. Translation: AAN12111.1 .
AE014296 Genomic DNA. Translation: AFH04310.1 .
AE014296 Genomic DNA. Translation: AFH04311.1 .
AY051916 mRNA. Translation: AAK93340.1 .
BT004507 mRNA. Translation: AAO42671.1 .
BT010120 mRNA. Translation: AAQ22589.1 .
BT046169 mRNA. Translation: ACI47091.1 .
RefSeqi NP_001246639.1. NM_001259710.2. [Q9VRP5-7 ]
NP_001246640.1. NM_001259711.2. [Q9VRP5-7 ]
NP_647986.3. NM_139729.3. [Q9VRP5-9 ]
NP_729092.1. NM_168132.2. [Q9VRP5-3 ]
NP_729093.2. NM_168133.2. [Q9VRP5-8 ]
NP_729094.1. NM_168134.2. [Q9VRP5-6 ]
NP_729095.1. NM_168135.2. [Q9VRP5-2 ]
UniGenei Dm.6825.

3D structure databases

ProteinModelPortali Q9VRP5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7227.FBpp0076805.

Protein family/group databases

MEROPSi C19.097.

Proteomic databases

PaxDbi Q9VRP5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0307975 ; FBpp0300344 ; FBgn0260936 . [Q9VRP5-7 ]
FBtr0307976 ; FBpp0300345 ; FBgn0260936 . [Q9VRP5-7 ]
GeneIDi 38648.
KEGGi dme:Dmel_CG5505.

Organism-specific databases

CTDi 38648.
FlyBasei FBgn0260936. scny.

Phylogenomic databases

eggNOGi COG5533.
GeneTreei ENSGT00750000117288.
InParanoidi Q86NM9.
KOi K11855.
OMAi ENEMDRG.
OrthoDBi EOG7XDBF6.

Miscellaneous databases

GenomeRNAii 38648.
NextBioi 809699.

Gene expression databases

Bgeei Q9VRP5.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Strain: Berkeley.
    Tissue: Embryo.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS C AND D).
    Strain: Berkeley.
    Tissue: Testis.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-513; SER-515; THR-658; THR-662; SER-672; SER-674; SER-747; SER-779; THR-782; SER-785; SER-819; THR-825; SER-843 AND THR-846, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.
  6. "Drosophila stem cells share a common requirement for the histone H2B ubiquitin protease scrawny."
    Buszczak M., Paterno S., Spradling A.C.
    Science 323:248-251(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATMS, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-181.

Entry informationi

Entry nameiUBP36_DROME
AccessioniPrimary (citable) accession number: Q9VRP5
Secondary accession number(s): M9NE30
, Q7YTX7, Q86NM9, Q8IQ57, Q8IQ58, Q8IQ59, Q8IQ60, Q960Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 16, 2014
Last modified: July 9, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi