ID   GOSR2_DROME             Reviewed;         216 AA.
AC   Q9VRL2; B5RJH1; Q8MRU9;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   24-JAN-2024, entry version 148.
DE   RecName: Full=Probable Golgi SNAP receptor complex member 2;
GN   Name=Membrin {ECO:0000312|FlyBase:FBgn0260856};
GN   ORFNames=CG4780 {ECO:0000312|FlyBase:FBgn0260856};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley;
RA   Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL   Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   IDENTIFICATION.
RX   PubMed=10908590; DOI=10.1083/jcb.150.2.f77;
RA   Littleton J.T.;
RT   "A genomic analysis of membrane trafficking and neurotransmitter release in
RT   Drosophila.";
RL   J. Cell Biol. 150:F77-F82(2000).
CC   -!- FUNCTION: Involved in transport of proteins from the cis/medial-Golgi
CC       to the trans-Golgi network. {ECO:0000250|UniProtKB:O35165}.
CC   -!- SUBUNIT: Part of a unique SNARE complex.
CC       {ECO:0000250|UniProtKB:O35165}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC       {ECO:0000250|UniProtKB:O35165}; Single-pass type IV membrane protein
CC       {ECO:0000255}. Golgi apparatus membrane {ECO:0000250|UniProtKB:O35165}.
CC       Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:O35165}.
CC       Note=Concentrated most in the intermediate compartment/cis-Golgi
CC       network and the cis-Golgi cisternae 1 and 2. Greatly reduced in
CC       concentration at the trans end of the Golgi apparatus.
CC       {ECO:0000250|UniProtKB:O35165}.
CC   -!- SIMILARITY: Belongs to the GOSR2 family. {ECO:0000305}.
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DR   EMBL; AE014296; AAF50783.1; -; Genomic_DNA.
DR   EMBL; AY119259; AAM51119.1; -; mRNA.
DR   EMBL; BT044445; ACH92510.1; -; mRNA.
DR   RefSeq; NP_647960.1; NM_139703.4.
DR   AlphaFoldDB; Q9VRL2; -.
DR   SMR; Q9VRL2; -.
DR   BioGRID; 64080; 28.
DR   DIP; DIP-21257N; -.
DR   IntAct; Q9VRL2; 14.
DR   STRING; 7227.FBpp0076849; -.
DR   PaxDb; 7227-FBpp0076849; -.
DR   DNASU; 38614; -.
DR   EnsemblMetazoa; FBtr0077145; FBpp0076849; FBgn0260856.
DR   GeneID; 38614; -.
DR   KEGG; dme:Dmel_CG4780; -.
DR   UCSC; CG4780-RA; d. melanogaster.
DR   AGR; FB:FBgn0260856; -.
DR   CTD; 38614; -.
DR   FlyBase; FBgn0260856; Membrin.
DR   VEuPathDB; VectorBase:FBgn0260856; -.
DR   eggNOG; KOG3251; Eukaryota.
DR   GeneTree; ENSGT00940000164674; -.
DR   HOGENOM; CLU_083740_0_1_1; -.
DR   InParanoid; Q9VRL2; -.
DR   OMA; RMRVDQL; -.
DR   OrthoDB; 5477092at2759; -.
DR   PhylomeDB; Q9VRL2; -.
DR   Reactome; R-DME-204005; COPII-mediated vesicle transport.
DR   Reactome; R-DME-5694530; Cargo concentration in the ER.
DR   Reactome; R-DME-6807878; COPI-mediated anterograde transport.
DR   Reactome; R-DME-6811438; Intra-Golgi traffic.
DR   BioGRID-ORCS; 38614; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 38614; -.
DR   PRO; PR:Q9VRL2; -.
DR   Proteomes; UP000000803; Chromosome 3L.
DR   Bgee; FBgn0260856; Expressed in saliva-secreting gland and 24 other cell types or tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR   GO; GO:0031201; C:SNARE complex; ISS:FlyBase.
DR   GO; GO:0005484; F:SNAP receptor activity; ISS:FlyBase.
DR   GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR   GO; GO:0035149; P:lumen formation, open tracheal system; HMP:FlyBase.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006906; P:vesicle fusion; ISS:FlyBase.
DR   GO; GO:0016192; P:vesicle-mediated transport; ISS:FlyBase.
DR   CDD; cd15863; SNARE_GS27; 1.
DR   Gene3D; 1.20.5.110; -; 1.
DR   Gene3D; 1.20.58.400; t-snare proteins; 1.
DR   InterPro; IPR027027; GOSR2/Membrin/Bos1.
DR   InterPro; IPR038407; v-SNARE_N_sf.
DR   PANTHER; PTHR21230:SF1; GOLGI SNAP RECEPTOR COMPLEX MEMBER 2; 1.
DR   PANTHER; PTHR21230; VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED; 1.
DR   Pfam; PF12352; V-SNARE_C; 1.
DR   PIRSF; PIRSF028865; Membrin-2; 1.
DR   SUPFAM; SSF58038; SNARE fusion complex; 1.
DR   Genevisible; Q9VRL2; DM.
PE   2: Evidence at transcript level;
KW   Coiled coil; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW   Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..216
FT                   /note="Probable Golgi SNAP receptor complex member 2"
FT                   /id="PRO_0000212553"
FT   TOPO_DOM        1..194
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        195..215
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        216
FT                   /note="Vesicular"
FT                   /evidence="ECO:0000255"
FT   COILED          62..103
FT                   /evidence="ECO:0000255"
FT   CONFLICT        125
FT                   /note="Y -> H (in Ref. 3; AAM51119)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   216 AA;  24833 MW;  3CD23EA81BA9851F CRC64;
     MESLYHQTNN VVKDIERDFQ RLSQLSAQES LDVENGIQLK ITQANANCDR LDVLLYKVPP
     SQRQSSKLRV DQLKYDLRHL QTSLQTARER RQRRMQEISE REQLLNHRFT ANSAQPEETR
     LQLDYELQHH TQLGNAHRGV DDMIASGSGI LESLISQRMT LGGAHKRIQA IGSTLGLSNH
     TMKLIERRLV EDRRIFIGGV VVTLLIIALI IYFLVL
//