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Protein

Ubiquitin thioesterase OTU1

Gene

CG4603

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei158By similarity1
Active sitei161NucleophileBy similarity1
Binding sitei266Substrate; via carbonyl oxygenBy similarity1
Active sitei267By similarity1
Active sitei341By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri317 – 341C2H2-typeAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-5689896. Ovarian tumor domain proteases.

Protein family/group databases

MEROPSiC85.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase OTU1 (EC:3.4.19.12By similarity)
Gene namesi
ORF Names:CG4603
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0035593. CG4603.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002823621 – 347Ubiquitin thioesterase OTU1Add BLAST347

Proteomic databases

PaxDbiQ9VRJ9.
PRIDEiQ9VRJ9.

Expressioni

Gene expression databases

BgeeiFBgn0035593.
GenevisibleiQ9VRJ9. DM.

Interactioni

Protein-protein interaction databases

BioGridi64071. 8 interactors.
IntActiQ9VRJ9. 1 interactor.
MINTiMINT-951773.
STRINGi7227.FBpp0303184.

Structurei

3D structure databases

ProteinModelPortaliQ9VRJ9.
SMRiQ9VRJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 87Ubiquitin-likeAdd BLAST83
Domaini150 – 274OTUPROSITE-ProRule annotationAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 89UBX-likeBy similarityAdd BLAST82
Regioni155 – 161Cys-loopBy similarity7
Regioni213 – 223Variable-loopBy similarityAdd BLAST11
Regioni263 – 267His-loopBy similarity5
Regioni290 – 295S2 siteBy similarity6

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri317 – 341C2H2-typeAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3288. Eukaryota.
COG5539. LUCA.
GeneTreeiENSGT00390000009989.
InParanoidiQ9VRJ9.
KOiK13719.
OrthoDBiEOG091G0CA6.
PhylomeDBiQ9VRJ9.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VRJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGSFSVKLK SKKGQFIVND LNEHTTLGEL KTKIVQATDI EATQLHVLVG
60 70 80 90 100
YPPKPLDLSQ QQEQRALKAV GINSGETLIV EEKAAPAPAA PVPGGTTVED
110 120 130 140 150
DEALARRLQA EEEAQLLQET AGGPVAQAAD YQLPVAPTES GPNGDFNGIL
160 170 180 190 200
LKKVVPADNS CLFTSIRFVL NGKVDNEGSE MMRHIIAQEV AADPQSYNDA
210 220 230 240 250
VLGKSNAEYC AWIQKADSWG GAIEVSILSN YYGIEIDVVD IQNAIINRFG
260 270 280 290 300
EDKNFGLRVF LLFDGIHYDP LYMETSPSAA PATIFPVEEL GVYQQAEQLA
310 320 330 340
NEAQSSRQYT NVDKFTLRCM QCDVRLVGQV QAQEHAKQTG HKNFGEI
Length:347
Mass (Da):37,823
Last modified:May 1, 2000 - v1
Checksum:iF8AFA9C679DD77E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87A → S in AAL39440 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50796.1.
AY069295 mRNA. Translation: AAL39440.1.
RefSeqiNP_001261436.1. NM_001274507.1.
NP_647951.2. NM_139694.4.
UniGeneiDm.2192.

Genome annotation databases

EnsemblMetazoaiFBtr0077164; FBpp0076867; FBgn0035593.
FBtr0331804; FBpp0304189; FBgn0035593.
GeneIDi38603.
KEGGidme:Dmel_CG4603.
UCSCiCG4603-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50796.1.
AY069295 mRNA. Translation: AAL39440.1.
RefSeqiNP_001261436.1. NM_001274507.1.
NP_647951.2. NM_139694.4.
UniGeneiDm.2192.

3D structure databases

ProteinModelPortaliQ9VRJ9.
SMRiQ9VRJ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64071. 8 interactors.
IntActiQ9VRJ9. 1 interactor.
MINTiMINT-951773.
STRINGi7227.FBpp0303184.

Protein family/group databases

MEROPSiC85.007.

Proteomic databases

PaxDbiQ9VRJ9.
PRIDEiQ9VRJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077164; FBpp0076867; FBgn0035593.
FBtr0331804; FBpp0304189; FBgn0035593.
GeneIDi38603.
KEGGidme:Dmel_CG4603.
UCSCiCG4603-RA. d. melanogaster.

Organism-specific databases

FlyBaseiFBgn0035593. CG4603.

Phylogenomic databases

eggNOGiKOG3288. Eukaryota.
COG5539. LUCA.
GeneTreeiENSGT00390000009989.
InParanoidiQ9VRJ9.
KOiK13719.
OrthoDBiEOG091G0CA6.
PhylomeDBiQ9VRJ9.

Enzyme and pathway databases

ReactomeiR-DME-5689896. Ovarian tumor domain proteases.

Miscellaneous databases

GenomeRNAii38603.
PROiQ9VRJ9.

Gene expression databases

BgeeiFBgn0035593.
GenevisibleiQ9VRJ9. DM.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTU1_DROME
AccessioniPrimary (citable) accession number: Q9VRJ9
Secondary accession number(s): Q8T9H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.