Q9VR52 (TILB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein TILB

Alternative name(s):
Touch insensitive larva B protein

Gene names

Name:	tilB
ORF Names:	CG14620

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cilium-specific protein required for cilia structures in Johnston's sensory organ. Plays a role in motility of cilia and flagella. Ref.3
Subcellular location	Cytoplasm. Cell projection › dendrite. Cell projection › cilium › flagellum. Note: Detected in the inner and outer dendritic segment and basal body. Ref.3
Tissue specificity	Expressed in chordotonal sense organs, Johnston's organ and sperm (at protein level). Expressed in ciliated cells. Expressed in the head and testis. Ref.3
Developmental stage	Expressed in ciliated cells of the chordotonal organs, neurons of Johnston's organ and femoral chordotonal organs, olfactory sensilla on the entenna in late stage embryos and larvae. Ref.3
Disruption phenotype	Larvae mutants are insensitive to touch and have defects in larval locomotion. Adult mutants show defects in motility and coordination and are deaf; lack antenna oscillations and mechanical amplification of stimulus-induced antennal vibrations. Mutant males can mate but are sterile because of amotile sperm; tail axonemes lacked the inner dynein arms. Ref.3
Sequence similarities	Belongs to the tilb protein family. Contains 1 CS domain. Contains 4 LRR (leucine-rich) repeats. Contains 1 LRRCT domain.

Ontologies

Keywords
Cellular component	Cell projection Cilium Cytoplasm Flagellum
Domain	Coiled coil Leucine-rich repeat Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ciliary cell motility Inferred from mutant phenotype Ref.3. Source: UniProtKB cilium axoneme assembly Inferred from mutant phenotype Ref.3. Source: FlyBase flagellar cell motility Inferred from mutant phenotype Ref.3. Source: UniProtKB male courtship behavior, veined wing generated song production Inferred from mutant phenotype. Source: FlyBase sensory perception of sound Inferred from mutant phenotype Ref.3. Source: FlyBase temperature compensation of the circadian clock Inferred from mutant phenotype. Source: FlyBase
Cellular component	cytoplasm Inferred from direct assay Ref.3. Source: FlyBase dendrite Inferred from direct assay Ref.3. Source: UniProtKB flagellum Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Protein TILB		PRO_0000414861
Regions
	Repeat	20 – 44	25	LRR 1
	Repeat	46 – 65	20	LRR 2
	Repeat	66 – 89	24	LRR 3
	Repeat	90 – 110	21	LRR 4
	Domain	128 – 146	19	LRRCT
	Domain	264 – 355	92	CS
	Coiled coil	170 – 196	27	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9VR52 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 069201406B0083F5	FASTA	395	45,608
	10 20 30 40 50 60 MVLITEELVR KKSEHNERLI STLEEISLHQ EDIEVIEHIQ NWCRDLKILL LQSNLIARLE 70 80 90 100 110 120 NLHKLKRLEY LNVAINNIER VENLEGCESL SKLDLTLNFI RELTSVESLC GNYNLRELVL 130 140 150 160 170 180 IGNPCVDYPH YRDYVVATLP QLNSLDCVEI TPSERLRALR ELSKNRSIIV QKQVEQDIER 190 200 210 220 230 240 DEQRIRVAKQ QSALAEHCAG IEDEEERIKA FWQAKSEHCP EIRTEIARQH RLGRERHETK 250 260 270 280 290 300 SPLDPLKPQR NLFAPCGRPY NLNQAKLPFK FRDEADHYLL QLEVYRHLDT SLIDVDVQTT 310 320 330 340 350 360 YTRVTVKKKI FQIAYSEEVK PDESTVQRSQ ITGHLVVNLK KLKVNELLIA KKSPTKSPAA 370 380 390 PFDAGKKDGK PEEAFHGGVV DISNICAPED LPDLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"Hearing in Drosophila requires TilB, a conserved protein associated with ciliary motility." Kavlie R.G., Kernan M.J., Eberl D.F. Genetics 185:177-188(2010) [PubMed: 20215474] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014298 Genomic DNA. Translation: AAF50954.1.
RefSeq	NP_608460.1. NM_134616.3.
3D structure databases
HSSP	HSSP built from PDB template 1DS9 based on UniProtKB Q9XHH2.
ProteinModelPortal	Q9VR52.
SMR	Q9VR52. Positions 18-160.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VR52. 2 interactions.
MINT	MINT-1571663.
STRING	Q9VR52.
Proteomic databases
PRIDE	Q9VR52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0077345; FBpp0077037; FBgn0014395.
GeneID	33130.
KEGG	dme:Dmel_CG14620.
NMPDR	fig|7227.3.peg.18939.
UCSC	CG14620-RA. d. melanogaster.
Organism-specific databases
CTD	33130.
FlyBase	FBgn0014395. tilB.
Phylogenomic databases
GeneTree	EMGT00050000003473.
InParanoid	Q9VR52.
OMA	QQLKWLD.
PhylomeDB	Q9VR52.
Gene expression databases
ArrayExpress	Q9VR52.
Bgee	Q9VR52.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR003603. U2A'_phosphoprotein32A_C. [Graphical view]
SMART	SM00446. LRRcap. 1 hit. [Graphical view]
PROSITE	PS51203. CS. False negative. PS51450. LRR. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	782055.

Entry information

Entry name

TILB_DROME

Accession

Primary (citable) accession number: Q9VR52

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 25, 2012
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project