Q9VQX4 (PNCB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate phosphoribosyltransferase
Short name=NAPRTase
EC=2.4.2.11

Gene names

ORF Names:

CG3714

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) By similarity.
Catalytic activity	Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway nicotinate nucleotide salvage Inferred from electronic annotation. Source: InterPro response to oxidative stress Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	nicotinate phosphoribosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB nicotinate-nucleotide diphosphorylase (carboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9VQX4-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9VQX4-2)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     304-304: K → KSRETEQANT...YLPKYVEKSF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Nicotinate phosphoribosyltransferase		PRO_0000315686
Natural variations
	Alternative sequence	304	1	K → KSRETEQANTEPKHINDIKS ENSPPKPKASPSTNGHQNGQ GVGHVNGHKTTNGHQNGSAQ NNGSRTPKDTDIQDTSTSTT TTKTTATATGTLKTTTTVSN GTAYLPKYVEKSF in isoform 2.		VSP_030614

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (A) [UniParc]. Last modified October 1, 2002. Version 2. Checksum: CA1E8C7954DD0F41	FASTA	555	62,165
	10 20 30 40 50 60 MNDRELGCAG GQFMDRGRMN QNGVVQPLLT DLYQITMAYA YWKSDKTDDT AVFDLFFRNN 70 80 90 100 110 120 PFHGEFTIFA GLEECLKFLD SFHYSQSDIE YLKQTLPEGI EHEFFEYLGN LTARDVTLYA 130 140 150 160 170 180 IDEGTVAFPR VPIIKIEGPL IIVQLLETTL LTLVNYASLM ATNAARYRMV AGKHVKLLEF 190 200 210 220 230 240 GLRRAQGPDG GLSASKYSYT GGFDGTSNVL AGKLFNIPVK GTHAHAYITS FSSIGELKTR 250 260 270 280 290 300 LIKHKQTGIL EDLLEHAVRH RALLSHLLDV STEESSEGEL AAMVSYAIAF PDGFMALVDT 310 320 330 340 350 360 YDVKRSGLLN FSAVALALND LGYHALGIRI DSGDLAYLSC LARETFEKVA ERFKVPWFNK 370 380 390 400 410 420 LTIVASNDIN EDTILSLNEQ GHKIDCFGIG THLVTCQRQP ALGCVYKLVE INGQPRIKLS 430 440 450 460 470 480 QDVEKVTMPG NKNAYRLYSA DGHALIDLLQ KVSEPPPAVG QKVLCRHPFQ ESKRAYVIPS 490 500 510 520 530 540 HVESLYKVYW KSGKICQQLP TLEQVREKVQ ISLKTLRNDH KRTLNPTPYK VAVSDNLYNF 550 IHDLWLQNAP IGELS
	Isoform 2 (C) [UniParc]. Checksum: 93CEC4DDF3DED751	FASTA	667	73,971
	10 20 30 40 50 60 MNDRELGCAG GQFMDRGRMN QNGVVQPLLT DLYQITMAYA YWKSDKTDDT AVFDLFFRNN 70 80 90 100 110 120 PFHGEFTIFA GLEECLKFLD SFHYSQSDIE YLKQTLPEGI EHEFFEYLGN LTARDVTLYA 130 140 150 160 170 180 IDEGTVAFPR VPIIKIEGPL IIVQLLETTL LTLVNYASLM ATNAARYRMV AGKHVKLLEF 190 200 210 220 230 240 GLRRAQGPDG GLSASKYSYT GGFDGTSNVL AGKLFNIPVK GTHAHAYITS FSSIGELKTR 250 260 270 280 290 300 LIKHKQTGIL EDLLEHAVRH RALLSHLLDV STEESSEGEL AAMVSYAIAF PDGFMALVDT 310 320 330 340 350 360 YDVKSRETEQ ANTEPKHIND IKSENSPPKP KASPSTNGHQ NGQGVGHVNG HKTTNGHQNG 370 380 390 400 410 420 SAQNNGSRTP KDTDIQDTST STTTTKTTAT ATGTLKTTTT VSNGTAYLPK YVEKSFRSGL 430 440 450 460 470 480 LNFSAVALAL NDLGYHALGI RIDSGDLAYL SCLARETFEK VAERFKVPWF NKLTIVASND 490 500 510 520 530 540 INEDTILSLN EQGHKIDCFG IGTHLVTCQR QPALGCVYKL VEINGQPRIK LSQDVEKVTM 550 560 570 580 590 600 PGNKNAYRLY SADGHALIDL LQKVSEPPPA VGQKVLCRHP FQESKRAYVI PSHVESLYKV 610 620 630 640 650 660 YWKSGKICQQ LPTLEQVREK VQISLKTLRN DHKRTLNPTP YKVAVSDNLY NFIHDLWLQN APIGELS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014134 Genomic DNA. Translation: AAF51037.2. AE014134 Genomic DNA. Translation: AAN11169.1. AE014134 Genomic DNA. Translation: AAN11170.1. AE014134 Genomic DNA. Translation: AAN11171.1. AE014134 Genomic DNA. Translation: AAN11172.1. AE014134 Genomic DNA. Translation: ABV53616.1. AY119468 mRNA. Translation: AAM50122.1.
RefSeq	NP_001097077.1. NM_001103607.2. NP_608818.4. NM_134974.5. NP_722961.1. NM_164572.2. NP_722962.1. NM_164573.2. NP_722963.1. NM_164574.2. NP_722964.1. NM_164575.2.
UniGene	Dm.11340.
3D structure databases
ProteinModelPortal	Q9VQX4.
SMR	Q9VQX4. Positions 106-517.
ModBase	Search...
Proteomic databases
PaxDb	Q9VQX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0077481; FBpp0077170; FBgn0031589.
GeneID	33626.
KEGG	dme:Dmel_CG3714.
UCSC	CG3714-RA. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0031589. CG3714.
Phylogenomic databases
eggNOG	COG1488.
GeneTree	ENSGT00390000002664.
InParanoid	Q9VQX4.
KO	K00763.
OMA	FWPEYLR.
OrthoDB	EOG44F4R7.
Enzyme and pathway databases
UniPathway	UPA00253; UER00457.
Gene expression databases
Bgee	Q9VQX4.
Family and domain databases
InterPro	IPR015977. Nic_PRibTrfase-like. IPR007229. Nic_PRibTrfase-rel. IPR006405. Nic_PRibTrfase_put. IPR002638. Quinolinate_PRibosylTrfase_C. [Graphical view]
PANTHER	PTHR11098:SF1. PTHR11098:SF1. 1 hit.
Pfam	PF04095. NAPRTase. 1 hit. [Graphical view]
PIRSF	PIRSF000484. NAPRT. 1 hit.
SUPFAM	SSF51690. Q_phspho_trans. 1 hit.
TIGRFAMs	TIGR01513. NAPRTase_put. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	33626.
NextBio	784493.

Entry information

Entry name

PNCB_DROME

Accession

Primary (citable) accession number: Q9VQX4
Secondary accession number(s): Q8MRQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2002
Last modified:	May 29, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project