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Protein

Putative apoptosis-inducing factor 1, mitochondrial

Gene

AIF

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probable NADH oxidoreductase (By similarity). Mitochondrial effector of cell death that plays roles in developmentally regulated cell death and normal mitochondrial function.By similarity1 Publication

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei295 – 2951FADBy similarity
Binding sitei300 – 3001FADBy similarity
Binding sitei358 – 3581FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei410 – 4101FADBy similarity
Binding sitei564 – 5641FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi261 – 2655FADBy similarity
Nucleotide bindingi580 – 5812FADBy similarity

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell redox homeostasis Source: InterPro
  • defense response to fungus Source: FlyBase
  • developmental programmed cell death Source: FlyBase
  • mitochondrial ATP synthesis coupled electron transport Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

FAD, Flavoprotein, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Putative apoptosis-inducing factor 1, mitochondrial (EC:1.1.1.-)
Short name:
DmAIF
Gene namesi
Name:AIF
ORF Names:CG7263
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0031392. AIF.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial intermembrane space Source: UniProtKB
  • mitochondrion Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Loss of zygotic expression results in decreased embryonic cell death and the persistence of differentiated neuronal cells along the ventral nerve cord at late embryonic stages. Embryos that do hatch undergo growth arrest at early larval stages, accompanied by mitochondrial respiratory dysfunction.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4242MitochondrionSequence analysisAdd
BLAST
Chaini43 – 739697Putative apoptosis-inducing factor 1, mitochondrialPRO_0000022029Add
BLAST

Proteomic databases

PaxDbiQ9VQ79.
PRIDEiQ9VQ79.

Expressioni

Developmental stagei

Expressed both maternally and zygotically throughout development with slightly lower levels during pupation.1 Publication

Gene expression databases

BgeeiQ9VQ79.
ExpressionAtlasiQ9VQ79. differential.
GenevisibleiQ9VQ79. DM.

Interactioni

Protein-protein interaction databases

BioGridi59619. 14 interactions.
IntActiQ9VQ79. 3 interactions.
STRINGi7227.FBpp0077504.

Structurei

3D structure databases

ProteinModelPortaliQ9VQ79.
SMRiQ9VQ79. Positions 252-737.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni257 – 564308FAD-dependent oxidoreductaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1346. Eukaryota.
COG0446. LUCA.
GeneTreeiENSGT00530000063416.
InParanoidiQ9VQ79.
KOiK04727.
OMAiYTPNPNE.
OrthoDBiEOG7C2R0V.
PhylomeDBiQ9VQ79.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR029324. AIF_C.
IPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
[Graphical view]
PfamiPF14721. AIF_C. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
SSF55424. SSF55424. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: Q9VQ79-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSIWGVRCLT QRFIRQAYIL ANRRLLGPVP QRSPPAYAPL RPAHSSLYQM
60 70 80 90 100
VKKRTLEART KLQANKYPNH QVCVTKPSTT PPVEEYETAV EGAGVPAYAN
110 120 130 140 150
AQFQAHSQSE PLFKVGFADV KSVCSAKDVL KSDSAKLSTS QPVLDSCKAT
160 170 180 190 200
SPCEEFKRKR KETTCQPCDE DGTAPGGGDG GDEECECRMK DLRLKCLLGA
210 220 230 240 250
LAALLAGGFL AWFMTRDTDD SEAKKAEAEE EERKRRLVAG LATSPPSSED
260 270 280 290 300
LPKHVPYLII GGGTAAFSAF RAIKSNDATA KVLMISNEFR KPYMRPPLSK
310 320 330 340 350
ELWYTPNPNE DPIKDYRFKQ WTGSERSLFF EPDEFFIDPE DLDDNANGGI
360 370 380 390 400
AVAQGFSVKK VDAQKRIVTL NDGYEISYDE CLIATGCAPK NLPMLRDAPP
410 420 430 440 450
SVLEKVMVYR TPDDFDRLRK LAAEKRSITI VGNGFIGSEL ACSLAHYSRE
460 470 480 490 500
NNGGKVYQVF QENANMSKVL PNYLSRWTTA KMEAQGVCVI PNASIRSAVR
510 520 530 540 550
DETNLKLELN NGMTLMSDVV VVCVGCTPNT DLAGPSRLEV DRSLGGFVVN
560 570 580 590 600
AELEARRNLY VAGDASCFFD PLLGRRRVEH HDHSVVSGRL AGENMTGAKK
610 620 630 640 650
PYQHQSMFWS DLGPEIGYEG IGLVDSSLPT VGVFALPSES ATRVDQLSES
660 670 680 690 700
SDSDVPETST SSSQSSKSDA GASQDGVTCD PDEAGNYGKG VIFYLKNDKI
710 720 730
VGILLWNLFN RIGLARTIIN QNKKYDDLNE VAKLFEIHA
Note: No experimental confirmation available.
Length:739
Mass (Da):81,217
Last modified:August 15, 2003 - v2
Checksum:i71184B1721AB93AF
GO
Isoform A (identifier: Q9VQ79-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-115: Missing.

Show »
Length:674
Mass (Da):74,050
Checksum:iEFFBAC7C5FC7E9DA
GO

Sequence cautioni

The sequence AAL13925.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti639 – 6391E → D in AAK93507 (PubMed:12537569).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei51 – 11565Missing in isoform A. 3 PublicationsVSP_007951Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF51299.2.
AE014134 Genomic DNA. Translation: AAN10444.2.
AY052083 mRNA. Translation: AAK93507.1.
AY058696 mRNA. Translation: AAL13925.1. Different initiation.
BT044192 mRNA. Translation: ACH92257.1.
RefSeqiNP_608649.2. NM_134805.4. [Q9VQ79-1]
NP_722765.2. NM_164456.3. [Q9VQ79-2]
UniGeneiDm.7891.

Genome annotation databases

EnsemblMetazoaiFBtr0077831; FBpp0077504; FBgn0031392. [Q9VQ79-2]
GeneIDi33390.
KEGGidme:Dmel_CG7263.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF51299.2.
AE014134 Genomic DNA. Translation: AAN10444.2.
AY052083 mRNA. Translation: AAK93507.1.
AY058696 mRNA. Translation: AAL13925.1. Different initiation.
BT044192 mRNA. Translation: ACH92257.1.
RefSeqiNP_608649.2. NM_134805.4. [Q9VQ79-1]
NP_722765.2. NM_164456.3. [Q9VQ79-2]
UniGeneiDm.7891.

3D structure databases

ProteinModelPortaliQ9VQ79.
SMRiQ9VQ79. Positions 252-737.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59619. 14 interactions.
IntActiQ9VQ79. 3 interactions.
STRINGi7227.FBpp0077504.

Proteomic databases

PaxDbiQ9VQ79.
PRIDEiQ9VQ79.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077831; FBpp0077504; FBgn0031392. [Q9VQ79-2]
GeneIDi33390.
KEGGidme:Dmel_CG7263.

Organism-specific databases

CTDi33390.
FlyBaseiFBgn0031392. AIF.

Phylogenomic databases

eggNOGiKOG1346. Eukaryota.
COG0446. LUCA.
GeneTreeiENSGT00530000063416.
InParanoidiQ9VQ79.
KOiK04727.
OMAiYTPNPNE.
OrthoDBiEOG7C2R0V.
PhylomeDBiQ9VQ79.

Miscellaneous databases

GenomeRNAii33390.
PROiQ9VQ79.

Gene expression databases

BgeeiQ9VQ79.
ExpressionAtlasiQ9VQ79. differential.
GenevisibleiQ9VQ79. DM.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR029324. AIF_C.
IPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
[Graphical view]
PfamiPF14721. AIF_C. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
SSF55424. SSF55424. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: Berkeley.
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.
  5. Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.

Entry informationi

Entry nameiAIFM1_DROME
AccessioniPrimary (citable) accession number: Q9VQ79
Secondary accession number(s): B5RIR8, Q95TL9, Q960F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 15, 2003
Last modified: June 8, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.