Reviewed, UniProtKB/Swiss-Prot Q9VPT1 (GR21A_DROME)

Names and origin

Protein names

Recommended name:
    Gustatory and odorant receptor 21a

Gene names

Name:	Gr21a
Synonyms:	GR21D.1
ORF Names:	CG13948

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable role in the gustatory response. Gr21a and Gr63a together are sufficient for olfactory carbon dioxide-chemosensation. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed in the adult labellar chemosensory neurons. Carbon dioxide-responsive neurons co-express Gr21a and Gr63a in a pair of chemosensory receptors at both larval and adult life stages. Ref.1 Ref.5
Sequence similarities	Belongs to the G-protein coupled receptor 7 family.
Sequence caution	The sequence AAF51461.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence ABE01237.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence ABE01237.1 differs from that shown. Reason: Frameshift at positions 333, 335 and 392.

Ontologies

Keywords
Biological process	Behavior Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW behavior Inferred from electronic annotation. Source: UniProtKB-KW detection of carbon dioxide Inferred from genetic interaction. Source: FlyBase response to carbon dioxide Ref.1 Inferred from genetic interaction. Source: FlyBase sensory perception of smell Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of taste Ref.5 Ref.6 Non-traceable author statement. Source: UniProtKB
Cellular component	cell soma Inferred from direct assay. Source: FlyBase dendrite Inferred from direct assay. Source: FlyBase integral to membrane Ref.5 Ref.6 Non-traceable author statement. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	taste receptor activity Ref.5 Ref.6 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Gustatory and odorant receptor 21a		PRO_0000216492
Regions
	Topological domain	1 – 114	114	Extracellular Potential
	Transmembrane	115 – 135	21	1 Potential
	Topological domain	136 – 153	18	Cytoplasmic Potential
	Transmembrane	154 – 174	21	2 Potential
	Topological domain	175 – 206	32	Extracellular Potential
	Transmembrane	207 – 227	21	3 Potential
	Topological domain	228 – 237	10	Cytoplasmic Potential
	Transmembrane	238 – 258	21	4 Potential
	Topological domain	259 – 312	54	Extracellular Potential
	Transmembrane	313 – 333	21	5 Potential
	Topological domain	334 – 345	12	Cytoplasmic Potential
	Transmembrane	346 – 366	21	6 Potential
	Topological domain	367 – 422	56	Extracellular Potential
	Transmembrane	423 – 443	21	7 Potential
	Topological domain	444 – 454	11	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	385	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9VPT1-1 [UniParc]. Last modified July 24, 2007. Version 3. Checksum: 3390E8F0E105C733	FASTA	454	52,773
	10 20 30 40 50 60 MTFLDRTMSF WAVSRGLTPP SKVVPMLNPN QRQFLEDEVR YREKLKLMAR GDAMEEVYVR 70 80 90 100 110 120 KQETVDDPLE LDKHDSFYQT TKSLLVLFQI MGVMPIHRNP PEKNLPRTGY SWGSKQVMWA 130 140 150 160 170 180 IFIYSCQTTI VVLVLRERVK KFVTSPDKRF DEAIYNVIFI SLLFTNFLLP VASWRHGPQV 190 200 210 220 230 240 AIFKNMWTNY QYKFFKTTGS PIVFPNLYPL TWSLCVFSWL LSIAINLSQY FLQPDFRLWY 250 260 270 280 290 300 TFAYYPIIAM LNCFCSLWYI NCNAFGTASR ALSDALQTTI RGEKPAQKLT EYRHLWVDLS 310 320 330 340 350 360 HMMQQLGRAY SNMYGMYCLV IFFTTIIATY GSISEIIDHG ATYKEVGLFV IVFYCMGLLY 370 380 390 400 410 420 IICNEAHYAS RKVGLDFQTK LLNINLTAVD AATQKEVEML LVAINKNPPI MNLDGYANIN 430 440 450 RELITTNISF MATYLVVLLQ FKITEQRRIG QQQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two chemosensory receptors together mediate carbon dioxide detection in Drosophila." Jones W.D., Cayirlioglu P., Grunwald Kadow I., Vosshall L.B. Nature 445:86-90(2007) [PubMed: 17167414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: Oregon-R. Tissue: Antenna.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
[5]	"Candidate taste receptors in Drosophila." Clyne P.J., Warr C.G., Carlson J.R. Science 287:1830-1834(2000) [PubMed: 10710312] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[6]	"Spatially restricted expression of candidate taste receptors in the Drosophila gustatory system." Dunipace L., Meister S., McNealy C., Amrein H. Curr. Biol. 11:822-835(2001) [PubMed: 11516643] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ989014 Genomic DNA. Translation: ABK97615.1. AE014134 Genomic DNA. Translation: AAF51461.2. Sequence problems. BT025007 mRNA. Translation: ABE01237.1. Sequence problems.
RefSeq	NP_523448.2.
UniGene	Dm.26698
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q9VPT1.
Genome annotation databases
Ensembl	FBgn0041250. Drosophila melanogaster. [Contig view]
GeneID	33251.
KEGG	dme:Dmel_CG13948.
NMPDR	fig|7227.3.peg.140.
Organism-specific databases
FlyBase	FBgn0041250. Gr21a.
Phylogenomic databases
HOGENOM	Q9VPT1.
Gene expression databases
ArrayExpress	Q9VPT1.
GermOnline	CG13948. Drosophila melanogaster.
Family and domain databases
InterPro	IPR013604. 7TM_chemorcpt. [Graphical view]
Pfam	PF08395. 7tm_7. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	782662.

Entry information

Entry name

GR21A_DROME

Accession

Primary (citable) accession number: Q9VPT1
Secondary accession number(s): A1C3L0, Q1WW99

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	July 24, 2007
Last modified:	June 16, 2009
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project