Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein Notchless

Gene

Nle

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in regulating Notch activity.1 Publication

GO - Molecular functioni

  • Notch binding Source: FlyBase

GO - Biological processi

  • Notch signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Notch signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Notchless1 Publication
Gene namesi
Name:NleImported
ORF Names:CG2863
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0021874. Nle.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Protein NotchlessPRO_0000424662Add
BLAST

Proteomic databases

PaxDbiQ9VPR4.

Expressioni

Gene expression databases

GenevisibleiQ9VPR4. DM.

Interactioni

Subunit structurei

Interacts with Notch (via cytoplasmic domain).1 Publication

GO - Molecular functioni

  • Notch binding Source: FlyBase

Protein-protein interaction databases

BioGridi59490. 5 interactions.
IntActiQ9VPR4. 1 interaction.
MINTiMINT-291987.
STRINGi7227.FBpp0077735.

Structurei

3D structure databases

ProteinModelPortaliQ9VPR4.
SMRiQ9VPR4. Positions 29-488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati117 – 15640WD 1Sequence analysisAdd
BLAST
Repeati159 – 19840WD 2Sequence analysisAdd
BLAST
Repeati202 – 24645WD 3Sequence analysisAdd
BLAST
Repeati249 – 28739WD 4Sequence analysisAdd
BLAST
Repeati329 – 37042WD 5Sequence analysisAdd
BLAST
Repeati373 – 41240WD 6Sequence analysisAdd
BLAST
Repeati415 – 45440WD 7Sequence analysisAdd
BLAST
Repeati457 – 48832WD 8Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the NLE1/RSA4 family.Sequence analysis
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0271. Eukaryota.
ENOG410XP3K. LUCA.
GeneTreeiENSGT00830000128308.
HOGENOMiHOG000238240.
InParanoidiQ9VPR4.
KOiK14855.
OMAiHINCLAW.
OrthoDBiEOG7SR4M7.
PhylomeDBiQ9VPR4.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VPR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAKKQKMQE TDTEQEATPH TIQARLVSDT GEEAGPPIDL PAGITTQQLG
60 70 80 90 100
LICNALLKNE EATPYLFFVG EDEIKKSLED TLDLASVDTE NVIDIVYQPQ
110 120 130 140 150
AVFKVRPVTR CTSSMPGHAE AVVSLNFSPD GAHLASGSGD TTVRLWDLNT
160 170 180 190 200
ETPHFTCTGH KQWVLCVSWA PDGKRLASGC KAGSIIIWDP ETGQQKGRPL
210 220 230 240 250
SGHKKHINCL AWEPYHRDPE CRKLASASGD GDCRIWDVKL GQCLMNIAGH
260 270 280 290 300
TNAVTAVRWG GAGLIYTSSK DRTVKMWRAA DGILCRTFSG HAHWVNNIAL
310 320 330 340 350
STDYVLRTGP FHPVKDRSKS HLSLSTEELQ ESALKRYQAV CPDEVESLVS
360 370 380 390 400
CSDDNTLYLW RNNQNKCVER MTGHQNVVND VKYSPDVKLI ASASFDKSVR
410 420 430 440 450
LWRASDGQYM ATFRGHVQAV YTVAWSADSR LIVSGSKDST LKVWSVQTKK
460 470 480
LAQELPGHAD EVFGVDWAPD GSRVASGGKD KVIKLWAY
Length:488
Mass (Da):53,810
Last modified:July 5, 2004 - v2
Checksum:iD4883202954A7785
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 292SD → Y in CAA10070 (PubMed:9857191).Curated
Sequence conflicti291 – 2911H → Q in CAA10070 (PubMed:9857191).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF51479.2.
AY089286 mRNA. Translation: AAL90024.1.
AJ012588 mRNA. Translation: CAA10070.1.
RefSeqiNP_477294.2. NM_057946.4.
UniGeneiDm.13475.

Genome annotation databases

EnsemblMetazoaiFBtr0078075; FBpp0077735; FBgn0021874.
GeneIDi33234.
KEGGidme:Dmel_CG2863.
UCSCiCG2863-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF51479.2.
AY089286 mRNA. Translation: AAL90024.1.
AJ012588 mRNA. Translation: CAA10070.1.
RefSeqiNP_477294.2. NM_057946.4.
UniGeneiDm.13475.

3D structure databases

ProteinModelPortaliQ9VPR4.
SMRiQ9VPR4. Positions 29-488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59490. 5 interactions.
IntActiQ9VPR4. 1 interaction.
MINTiMINT-291987.
STRINGi7227.FBpp0077735.

Proteomic databases

PaxDbiQ9VPR4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078075; FBpp0077735; FBgn0021874.
GeneIDi33234.
KEGGidme:Dmel_CG2863.
UCSCiCG2863-RA. d. melanogaster.

Organism-specific databases

CTDi33234.
FlyBaseiFBgn0021874. Nle.

Phylogenomic databases

eggNOGiKOG0271. Eukaryota.
ENOG410XP3K. LUCA.
GeneTreeiENSGT00830000128308.
HOGENOMiHOG000238240.
InParanoidiQ9VPR4.
KOiK14855.
OMAiHINCLAW.
OrthoDBiEOG7SR4M7.
PhylomeDBiQ9VPR4.

Miscellaneous databases

GenomeRNAii33234.
NextBioi782583.
PROiQ9VPR4.

Gene expression databases

GenevisibleiQ9VPR4. DM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley1 Publication.
    Tissue: Testis1 Publication.
  4. "Notchless encodes a novel WD40-repeat-containing protein that modulates Notch signaling activity."
    Royet J., Bouwmeester T., Cohen S.M.
    EMBO J. 17:7351-7360(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-488, FUNCTION, INTERACTION WITH NOTCH.

Entry informationi

Entry nameiNLE_DROME
AccessioniPrimary (citable) accession number: Q9VPR4
Secondary accession number(s): O96995, Q8T4A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 11, 2013
Last sequence update: July 5, 2004
Last modified: February 17, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

The name 'Notchless' derives from the ability to suppress the wing notching caused by some Notch mutant alleles.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.