Q9VPC0 (KP58_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PITSLRE
EC=2.7.11.22

Alternative name(s):
Cell division cycle 2-like

Gene names

Name:	Pitslre
ORF Names:	CG4268

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	952 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a negative regulator of the normal cell cycle progression. May function in regulating proliferation by the phosphorylation and subsequent plasma membrane targeting of galactosyltransferase By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + a protein tyrosine = ADP + protein tyrosine phosphate.
Subcellular location	Nucleus Potential.
Tissue specificity	Present throughout the early embryo. In late embryos levels are highest in the CNS. Ref.1
Developmental stage	Expressed both maternally and zygotically. Highest levels in early embryogenesis (0-6 hours), low levels during later embryogenesis, moderate levels in pupae and adults. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAM75018.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from mutant phenotype PubMed 14527345. Source: FlyBase cell cycle Inferred from mutant phenotype PubMed 14527345. Source: FlyBase regulation of cell shape Inferred from mutant phenotype PubMed 14527345. Source: FlyBase
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Inferred from sequence or structural similarity Ref.6Ref.1. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 952	952	Serine/threonine-protein kinase PITSLRE		PRO_0000086160
Regions
	Domain	558 – 851	294	Protein kinase
	Nucleotide binding	564 – 572	9	ATP By similarity
	Motif	51 – 56	6	Nuclear localization signal Potential
	Compositional bias	92 – 146	55	His-rich
	Compositional bias	386 – 504	119	Ser-rich
Sites
	Active site	685	1	Proton acceptor By similarity
	Binding site	587	1	ATP By similarity
Amino acid modifications
	Modified residue	439	1	Phosphoserine Ref.7
	Modified residue	442	1	Phosphoserine Ref.7
	Modified residue	447	1	Phosphotyrosine Ref.7
	Modified residue	449	1	Phosphoserine Ref.7
	Modified residue	881	1	Phosphoserine Ref.7
	Modified residue	886	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	69	1	D → E in CAA67863. Ref.1
	Sequence conflict	223	1	V → A in CAA67863. Ref.1
	Sequence conflict	283	1	I → S in CAA67863. Ref.1
	Sequence conflict	492	1	V → A in CAA67863. Ref.1
	Sequence conflict	584	1	V → E Ref.6
	Sequence conflict	603	1	L → R Ref.6
	Sequence conflict	612	1	G → GG Ref.6
	Sequence conflict	670	1	Missing Ref.6
	Sequence conflict	680	1	Missing Ref.6
	Sequence conflict	717	1	Missing Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9VPC0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 9CBDE8D459D0713D	FASTA	952	108,838
	10 20 30 40 50 60 MVNSSGSEDG QLRSPNDVHY HSRGEEDEHE GDADALYIQP PQASRESGSG PRREKKKHSR 70 80 90 100 110 120 ERRRHKERDD VGGAALALER DHRYDYRSRE EHYHHHQRER SSNAAAAYAK HHLGHAYHYP 130 140 150 160 170 180 QPPQQQQQPL PPAPSYAAHH YHHHQHLSGA RAAPREYHSY PSGYHSGSRH GDYPMEEPTR 190 200 210 220 230 240 RSSKYAESKD AESLEQDLRS RLLKKRHNYV KDYETEENYE HRVERSDRRE GGRKERERTV 250 260 270 280 290 300 RSTHKQNRHD RVIELLDSPE QEHHHQHQHK SHRSKWREEV EVIRRKVPED LELLARREKL 310 320 330 340 350 360 LAAERESRQR KQTAREELEA RRELLRERNE HSDALSPTTV AASVTAGLNI HVKRKSKPDN 370 380 390 400 410 420 YEKEIKLKKR REDDIEVIRD DDDEESEESD SNEEVPEQDS EGSATESGSE DSYASKKKSK 430 440 450 460 470 480 IKSKSQLEDD DEDLPLPDSP LSVGELYKSP KQRQRSRSVS SKSSSQSSRS SRSRSRSRSQ 490 500 510 520 530 540 SSLEDEVDRQ DVGADASPSS STRSEERGMT QEQPEEKPEE KLKEKQKSLE EQIPCDDKGI 550 560 570 580 590 600 PLPNYYPGVQ GCRSVEEFQC LNRIEEGTYG VVYRAKDKRT NEIVALKRLK MEKEKEGFPI 610 620 630 640 650 660 TSLREINTLL KGQHPNIVTV REIVVGSNMD KIFIVMDYVE HDLKSLMETM KNRKQSFFPG 670 680 690 700 710 720 EVKCLTQQLL RAVAHLHDNW ILHRDLKTSN LLLSHKGILK VGDFGLAREY GSPIKKYTSL 730 740 750 760 770 780 VVTLWYRAPE LLLCSPVYST PIDVWSVGCI FAEFLQMLPL FPGKSEIDEL NRIFKELGTP 790 800 810 820 830 840 NEKIWPGYTE LPAVKNMLSQ NSQFTEYPVS QLRKHFQEKT SEMGLSLLQG LLTYDPKQRL 850 860 870 880 890 900 SADAALKHGF FKELPLPIDP SMFPTWPAKS ELGARKAQAS SPKPPSGGSQ FKQLGRDEPI 910 920 930 940 950 IVGPGNKLSS GIITGNKKSH GAGGSSASTG FVLNAGITQR QLAMGPGFSL KF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel members of the cdc2-related kinase family in Drosophila: cdk4/6, cdk5, PFTAIRE, and PITSLRE kinase." Sauer K., Weigmann K., Sigrist S., Lehner C.F. Mol. Biol. Cell 7:1759-1769(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 22-952. Strain: Berkeley. Tissue: Head.
[6]	"Primary structure, expression, and signal-dependent tyrosine phosphorylation of a Drosophila homolog of extracellular signal-regulated kinase." Biggs W.H. III, Zipursky S.L. Proc. Natl. Acad. Sci. U.S.A. 89:6295-6299(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 571-722. Tissue: Imaginal disk.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-439; SER-442; TYR-447; SER-449; SER-881 AND SER-886, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X99513 mRNA. Translation: CAA67863.1. AE014296 Genomic DNA. Translation: AAF51635.1. AE014296 Genomic DNA. Translation: AAN12141.1. BT016092 mRNA. Translation: AAV36977.1. AY128425 mRNA. Translation: AAM75018.1. Sequence problems.
RefSeq	NP_001246851.1. NM_001259922.2. NP_001246852.1. NM_001259923.2. NP_001262122.1. NM_001275193.1. NP_649251.2. NM_140994.4. NP_730563.1. NM_168869.3.
UniGene	Dm.1019.
3D structure databases
ProteinModelPortal	Q9VPC0.
SMR	Q9VPC0. Positions 555-853.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VPC0. 2 interactions.
Proteomic databases
PaxDb	Q9VPC0.
PRIDE	Q9VPC0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0078247; FBpp0077905; FBgn0016696. FBtr0078250; FBpp0077908; FBgn0016696.
GeneID	40292.
KEGG	dme:Dmel_CG4268.
Organism-specific databases
CTD	40292.
FlyBase	FBgn0016696. Pitslre.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00690000102162.
InParanoid	Q9VPC0.
KO	K08818.
OMA	IFAEFLQ.
OrthoDB	EOG4KH197.
PhylomeDB	Q9VPC0.
Enzyme and pathway databases
BRENDA	2.7.11.22. 1994.
Gene expression databases
Bgee	Q9VPC0.
GermOnline	CG4268. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	40292.
NextBio	818015.

Entry information

Entry name

KP58_DROME

Accession

Primary (citable) accession number: Q9VPC0
Secondary accession number(s): Q5U100 , Q8MQR7, Q94889, Q9TXB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project