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Protein

Cyclin-dependent kinase 12

Gene

Cdk12

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cyclin-dependent kinase which displays CTD kinase activity: hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit, thereby acting as a key regulator of transcription elongation.1 Publication

Catalytic activityi

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.1 Publication
ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei833ATPPROSITE-ProRule annotation1
Active sitei936Proton acceptorPROSITE-ProRule annotation1
Binding sitei1118ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi810 – 818ATPPROSITE-ProRule annotation9
Nucleotide bindingi891 – 896ATPPROSITE-ProRule annotation6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin binding Source: UniProtKB
  • cyclin-dependent protein serine/threonine kinase activity Source: FlyBase
  • RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB
  • transcription factor binding Source: GO_Central
  • transcription regulatory region DNA binding Source: GO_Central

GO - Biological processi

  • phosphorylation of RNA polymerase II C-terminal domain Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • transcription elongation from RNA polymerase II promoter Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-DME-6798695 Neutrophil degranulation
SignaLinkiQ9VP22

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 12 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 12
Short name:
dCdk12
Gene namesi
Name:Cdk12
ORF Names:CG7597
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi

Organism-specific databases

FlyBaseiFBgn0037093 Cdk12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003728591 – 1157Cyclin-dependent kinase 12Add BLAST1157

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei106Phosphothreonine1 Publication1
Modified residuei184Phosphothreonine1 Publication1
Modified residuei190Phosphoserine1 Publication1
Modified residuei192Phosphoserine1 Publication1
Modified residuei217Phosphothreonine1 Publication1
Modified residuei280Phosphoserine1 Publication1
Modified residuei283Phosphothreonine1 Publication1
Modified residuei291Phosphoserine1 Publication1
Modified residuei301Phosphoserine1 Publication1
Modified residuei314Phosphoserine1 Publication1
Modified residuei353Phosphoserine1 Publication1
Modified residuei365Phosphothreonine1 Publication1
Modified residuei487Phosphoserine1 Publication1
Modified residuei492Phosphoserine1 Publication1
Modified residuei553Phosphoserine1 Publication1
Modified residuei730Phosphoserine1 Publication1
Modified residuei743Phosphoserine1 Publication1
Modified residuei747Phosphoserine1 Publication1
Modified residuei755Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VP22
PRIDEiQ9VP22

PTM databases

iPTMnetiQ9VP22

Expressioni

Gene expression databases

BgeeiFBgn0037093
ExpressionAtlasiQ9VP22 baseline and differential
GenevisibleiQ9VP22 DM

Interactioni

Subunit structurei

Interacts with cyclin CycK.1 Publication

GO - Molecular functioni

  • cyclin binding Source: UniProtKB
  • transcription factor binding Source: GO_Central

Protein-protein interaction databases

BioGridi65630, 2 interactors
IntActiQ9VP22, 11 interactors
STRINGi7227.FBpp0078013

Structurei

3D structure databases

ProteinModelPortaliQ9VP22
SMRiQ9VP22
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini804 – 1098Protein kinasePROSITE-ProRule annotationAdd BLAST295

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0600 Eukaryota
ENOG410XPIR LUCA
GeneTreeiENSGT00890000139396
InParanoidiQ9VP22
KOiK08819
OMAiQKKTHRR
OrthoDBiEOG091G08Z8
PhylomeDBiQ9VP22

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VP22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHASSAAATA LVEYSDVSSE DFSDQEAGDL DADAGKGAGN IKKPKPAPDN
60 70 80 90 100
QFSKGRLDAK PDKEGYDNYR SRRAEDSSDP VAAGSRQTSS SEATNPREEP
110 120 130 140 150
SQASNTSKDE LWGREIYMSS DSIDTDELEA EMKRQKRKKQ KKEKHKHKSK
160 170 180 190 200
KKSKKRKKKR AKSYSSIDSM SDNDINALLD RRYTPPTAPS KSNERTVSAA
210 220 230 240 250
PSSFTPHNLK ESSSPATPPP VRRPNTNSNY YGESSLETAN SALGSNLQVT
260 270 280 290 300
VTNKQSISNR LRSPPPSSRS SGNGPRFGNS PRTPPPSHYS SSGGGGVGSG
310 320 330 340 350
SVVRDSRSSR YVNSPHKEDV SAHHRSSHDH GYQGRYSGAG SSSHDTRKVK
360 370 380 390 400
RLSPELDRYN HQPSTPPHKR RKFSDGREVG LGNFEHSRHH SGKYERYSRD
410 420 430 440 450
RYSRRSSRSP SVQHSRSRQS PSGGLSSGSN AFRHGGSHKH KYGTTVSSTP
460 470 480 490 500
SHTTRTSKRA SGTGTSGDRY SRSPRTSSRY MESSPPSPVG ASGSHHYHHR
510 520 530 540 550
RSPRMRQRTR GDSRRRSPSS ASSESSASRS RSPTSRDLKH KREEYIKKIS
560 570 580 590 600
ETSLFAELVK DRHKRQKALK EIIERQEENS NSNSNGALTI NDNSSSVDGN
610 620 630 640 650
TPNAADGRSA PGSGTPAAAS TTSNGLQALG SKPDLDLNNI PMPNKQNDSV
660 670 680 690 700
VSNPASNADV PDSVAQLKQP LLVPPFSASK NNIKPKSLTS LPLPPGMNVL
710 720 730 740 750
DLAGARSPSP GQKKESDEKN VTSSGSANKS VLNLPMPPVI PGSEELSGDD
760 770 780 790 800
DVIDSPEDFD APAVGTVHGH GGGPGTTRQR PVILNRRDSR NNVRDWGERC
810 820 830 840 850
VDVFEMIAQI GEGTYGQVYK ARDHHTNDMV ALKKVRLEHE KEGFPITAVR
860 870 880 890 900
EIKILRQLNH RNIVNLHEIV TDKQDAVEFR KDKGSFYLVF EYMDHDLMGL
910 920 930 940 950
LESGMVDFNE ENNASIMKQL LDGLNYCHKK NFLHRDIKCS NILMNNRGKV
960 970 980 990 1000
KLADFGLARL YNADDRERPY TNKVITLWYR PPELLLGEER YGPSIDVWSC
1010 1020 1030 1040 1050
GCILGELFVK RPLFQANAEM AQLETISKIC GSPVPAVWPN VIKLPLFHTL
1060 1070 1080 1090 1100
KQKKTHRRRL REDFEFMPAP ALDLLDKMLD LDPDKRITAE DALRSPWLRK
1110 1120 1130 1140 1150
INPDEMPTPQ LPTWQDCHEL WSKKRRRQMR EQQESLPPTV IASTKYQQHG

ATMVGDA
Length:1,157
Mass (Da):128,333
Last modified:May 1, 2000 - v1
Checksum:iB37ADCFBCAB4E9F3
GO

Sequence cautioni

The sequence AAL28383 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188A → V in AAL39951 (PubMed:12537569).Curated1
Sequence conflicti1099R → K in AAL28383 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AAF51738.1
AE014296 Genomic DNA Translation: AAN12171.1
AY060835 mRNA Translation: AAL28383.1 Different initiation.
AY069806 mRNA Translation: AAL39951.1
BT058001 mRNA Translation: ACM16711.1
RefSeqiNP_001262167.1, NM_001275238.1
NP_649325.2, NM_141068.4
NP_730643.1, NM_168912.2
UniGeneiDm.1943

Genome annotation databases

EnsemblMetazoaiFBtr0078357; FBpp0078013; FBgn0037093
FBtr0078358; FBpp0078014; FBgn0037093
FBtr0332716; FBpp0304962; FBgn0037093
GeneIDi40385
KEGGidme:Dmel_CG7597
UCSCiCG7597-RA d. melanogaster

Entry informationi

Entry nameiCDK12_DROME
AccessioniPrimary (citable) accession number: Q9VP22
Secondary accession number(s): Q8T9E1, Q95SE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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