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Protein

Mediator of RNA polymerase II transcription subunit 1

Gene

MED1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for activated transcription of the MtnA, MtnB and MtnD genes.By similarity1 Publication

GO - Molecular functioni

  • RNA polymerase II transcription cofactor activity Source: UniProtKB

GO - Biological processi

  • transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription initiation from RNA polymerase II promoter Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-212436. Generic Transcription Pathway.
R-DME-383280. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 1
Alternative name(s):
Mediator complex subunit 1
dTRAP220
Gene namesi
Name:MED1
Synonyms:Trap220
ORF Names:CG7162
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0037109. MED1.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: UniProtKB
  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003020231 – 1475Mediator of RNA polymerase II transcription subunit 1Add BLAST1475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei830Phosphoserine1 Publication1
Modified residuei834Phosphoserine1 Publication1
Modified residuei854Phosphoserine1 Publication1
Modified residuei858Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VP05.
PRIDEiQ9VP05.

PTM databases

iPTMnetiQ9VP05.

Expressioni

Gene expression databases

BgeeiFBgn0037109.
ExpressionAtlasiQ9VP05. baseline.
GenevisibleiQ9VP05. DM.

Interactioni

Subunit structurei

Component of the Mediator complex.By similarity

Protein-protein interaction databases

BioGridi65647. 2 interactors.
IntActiQ9VP05. 3 interactors.
MINTiMINT-1018274.
STRINGi7227.FBpp0078118.

Structurei

3D structure databases

ProteinModelPortaliQ9VP05.
SMRiQ9VP05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi529 – 577Gly-richAdd BLAST49
Compositional biasi756 – 1346Ser-richAdd BLAST591
Compositional biasi985 – 1129Ala-richAdd BLAST145

Sequence similaritiesi

Belongs to the Mediator complex subunit 1 family.Curated

Phylogenomic databases

eggNOGiENOG410IEFR. Eukaryota.
ENOG410XR2E. LUCA.
GeneTreeiENSGT00660000095569.
InParanoidiQ9VP05.
KOiK15144.
OMAiEFKLHGV.
OrthoDBiEOG091G035E.
PhylomeDBiQ9VP05.

Family and domain databases

InterProiIPR019680. Mediator_Med1.
[Graphical view]
PfamiPF10744. Med1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VP05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSNAKSSG TGFGSHIPSI EEKNKQIQQE TMMEKLRAKY RNKPKSYEEI
60 70 80 90 100
KKSVRMYFIE KHYPLDPLCK ATLQSALDKL QHYIKVTSRH GLVERLESLS
110 120 130 140 150
RQLGLKFMED QQLLFISTDM FYVEILLDAA GSLSDVKVHH ECKIEQQSSE
160 170 180 190 200
LVACLKSGDF ADFTVQLEGL SSIYQLNAEP KVKKKAFVAL QAMETDIQSL
210 220 230 240 250
YQLHLQGHSG DSYSLMTSSS VGLVLPRRGG HPMRLTYFCP PLHLPEGDPK
260 270 280 290 300
LASGDFTIDQ VMRSSYGLSA TINLEGSSAN KLQTLPTVTL VRDAQTGLEV
310 320 330 340 350
PTYAQLNQNN SLLMPATFVL RLNKPMPVCL ESLKALGLPG LDSVATPPGP
360 370 380 390 400
PTTVLNLIVQ TASKQAIKNT QRGLYVNLPK ETHCYFFTDN RKLQGTLVSS
410 420 430 440 450
LPFTEPAQVP RIVAFLKKQA LFYTLLASCV REQQKQYNDM DSTVILEVTA
460 470 480 490 500
VSFNQITVEL QHPYEESLAT VDFLLEDGQP TCSVYCLTNE YELLSQKLTR
510 520 530 540 550
TARKVVSIPM VIYKLLKCWD EEHEFKLHGA IGPGSGSGAI GGGGMSGGGP
560 570 580 590 600
VSGVGNNFSQ FSMDTPTPSD GSLPGGGFAN INNLKMDAKS RSLADAFAAS
610 620 630 640 650
TSAAAAIAGL INLKRETDPQ SGSSASGTTV SGSSSSSGSA KTSDHDIADK
660 670 680 690 700
YKNIWKDKTP NLKHCVSITP IPGDGKSGSA GGVSGVEVQR TGGIEIIPLN
710 720 730 740 750
AQAAIAGGGV TASSSATPTT ITITPITGKD PSKDSTKKST AASAGVGVAA
760 770 780 790 800
KRPHESSTSS SSTSGCSGSG SSMSSSASSG SSDTQKEKKR KKKRDDSPMG
810 820 830 840 850
PPEKIYSRQN SPAGGADASA TGGVVRKFSS PSSSPKAGGG GQGLMAGVPT
860 870 880 890 900
ARPSPKHSPV YSSPKHNTAS NSPKSPFGTH SPKHGSSGKP SMSTLKSAAT
910 920 930 940 950
AATILSPKGD KSSSAVGNTS SGPSASSGSS GATGLVRSFA SVGAPPPPPP
960 970 980 990 1000
IPPLASSSGS ISSSQSLKKE KTSSASGSSS TSSSATAGVA SGGGISPASV
1010 1020 1030 1040 1050
AAAVAALKSS QQQMKSVASL SHLAAGGGLG SYAAPSGAGA SGAAAVVVGA
1060 1070 1080 1090 1100
GAGAGAGASG LELSALRKGM AGGAVSLMTS TAALAPTIPA PTTTVAAGSA
1110 1120 1130 1140 1150
ASLVSPVSAV VGQGQETAGA AAAATLATAT ILQQQPQPGA APTSSCLTTS
1160 1170 1180 1190 1200
GGSSDSAGSI NPAGASTEYM VKPSSQEGLK LTINKTGSSK SSGTGSGSSS
1210 1220 1230 1240 1250
SSGLQAKAKS SSSGATSFAG STGSTKKQHT GLKPGVNSGP ASKKATAAVS
1260 1270 1280 1290 1300
SATASSSKHF FQKANSSGNL SSKLSGSGSG GGIPLTKSNS TNSFQEHNAP
1310 1320 1330 1340 1350
RRRPSMGALA SGSSGGGSGQ RKLGSASGGG SGSSGSVSPA LSGSMSQPPP
1360 1370 1380 1390 1400
RFDHHTDMMT ILQYASPTMA ASMEGFIKGL HNKFQIPKLS QRGSGGNTTS
1410 1420 1430 1440 1450
GRSTPSGSSE PALAGTSSSI LGPIASSTGL TEPEAKPPVP PSQSGNEGLL
1460 1470
NLSSTAGTPS ADGIDEELLA SLAGE
Length:1,475
Mass (Da):149,531
Last modified:October 1, 2002 - v2
Checksum:i273E6CB3949DC6CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF289996 mRNA. Translation: AAG02461.1.
AE014296 Genomic DNA. Translation: AAF51757.2.
BT044447 mRNA. Translation: ACH92512.1.
RefSeqiNP_649341.1. NM_141084.3.
UniGeneiDm.6444.

Genome annotation databases

EnsemblMetazoaiFBtr0078464; FBpp0078118; FBgn0037109.
GeneIDi40403.
KEGGidme:Dmel_CG7162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF289996 mRNA. Translation: AAG02461.1.
AE014296 Genomic DNA. Translation: AAF51757.2.
BT044447 mRNA. Translation: ACH92512.1.
RefSeqiNP_649341.1. NM_141084.3.
UniGeneiDm.6444.

3D structure databases

ProteinModelPortaliQ9VP05.
SMRiQ9VP05.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65647. 2 interactors.
IntActiQ9VP05. 3 interactors.
MINTiMINT-1018274.
STRINGi7227.FBpp0078118.

PTM databases

iPTMnetiQ9VP05.

Proteomic databases

PaxDbiQ9VP05.
PRIDEiQ9VP05.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078464; FBpp0078118; FBgn0037109.
GeneIDi40403.
KEGGidme:Dmel_CG7162.

Organism-specific databases

CTDi5469.
FlyBaseiFBgn0037109. MED1.

Phylogenomic databases

eggNOGiENOG410IEFR. Eukaryota.
ENOG410XR2E. LUCA.
GeneTreeiENSGT00660000095569.
InParanoidiQ9VP05.
KOiK15144.
OMAiEFKLHGV.
OrthoDBiEOG091G035E.
PhylomeDBiQ9VP05.

Enzyme and pathway databases

ReactomeiR-DME-212436. Generic Transcription Pathway.
R-DME-383280. Nuclear Receptor transcription pathway.

Miscellaneous databases

ChiTaRSiMED1. fly.
GenomeRNAii40403.
PROiQ9VP05.

Gene expression databases

BgeeiFBgn0037109.
ExpressionAtlasiQ9VP05. baseline.
GenevisibleiQ9VP05. DM.

Family and domain databases

InterProiIPR019680. Mediator_Med1.
[Graphical view]
PfamiPF10744. Med1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED1_DROME
AccessioniPrimary (citable) accession number: Q9VP05
Secondary accession number(s): B5RJH3, Q9GYW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.