Trxr-2

Functionⁱ

Thioredoxin system is a major player in glutathione metabolism, due to the demonstrated absence of a glutathione reductase. Functionally interacts with the Sod/Cat reactive oxidation species (ROS) defense system and thereby has a role in preadult development and life span. Lack of a glutathione reductase suggests antioxidant defense in Drosophila, and probably in related insects, differs fundamentally from that in other organisms.By similarity

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityⁱ

Thioredoxin + NADP⁺ = thioredoxin disulfide + NADPH.By similarity

Cofactorⁱ

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	489	Proton acceptorBy similarity		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	62 – 79	FADBy similarityAdd BLAST		18

GO - Molecular functionⁱ

electron transfer activity Source: InterPro
flavin adenine dinucleotide binding Source: InterPro
thioredoxin-disulfide reductase activity
Source: FlyBase
Inferred from direct assayⁱ
- 14569031

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell redox homeostasis
Source: FlyBase
Inferred from sequence or structural similarityⁱ
- 11158675
response to oxygen radical Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Ligand	FAD, Flavoprotein, NADP

Enzyme and pathway databases

Reactomeⁱ	R-DME-3299685 Detoxification of Reactive Oxygen Species

Reactomeⁱ

R-DME-3299685 Detoxification of Reactive Oxygen Species

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Thioredoxin reductase 2, mitochondrial (EC:1.8.1.9) Short name: TrxR-2
Gene namesⁱ	Name:Trxr-2 ORF Names:CG11401
Organismⁱ	Drosophila melanogaster (Fruit fly)Imported
Taxonomic identifierⁱ	7227 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophila › Sophophora › melanogaster group › melanogaster subgroup
Proteomesⁱ	UP000000803 Componentⁱ: Chromosome 3L

Organism-specific databases

Drosophila genome database More...FlyBaseⁱ	FBgn0037170 Trxr-2

FlyBaseⁱ

FBgn0037170 Trxr-2

Keywords - Cellular componentⁱ

Mitochondrion

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000030293	? – 516	Thioredoxin reductase 2, mitochondrial
Transit peptideⁱ	1 – ?	MitochondrionSequence analysis

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Disulfide bondⁱ	79 ↔ 84	Redox-activeBy similarity

Keywords - PTMⁱ

Disulfide bond

Proteomic databases

PaxDbⁱ	Q9VNT5
PRIDEⁱ	Q9VNT5

Expressionⁱ

Gene expression databases

Bgeeⁱ	FBgn0037170
Genevisibleⁱ	Q9VNT5 DM

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

Protein-protein interaction databases

BioGridⁱ	657092 interactors.
Database of interacting proteins More...DIPⁱ	DIP-19796N
IntActⁱ	Q9VNT5 1 interactor.
STRINGⁱ	7227.FBpp0078166

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9VNT5
SMRⁱ	Q9VNT5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.Curated

Keywords - Domainⁱ

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGⁱ	KOG0405 Eukaryota COG1249 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000007578
InParanoidⁱ	Q9VNT5
KEGG Orthology (KO) More...KOⁱ	K22182
OMAⁱ	GLECACF
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03IU
PhylomeDBⁱ	Q9VNT5

Family and domain databases

Gene3Dⁱ	3.30.390.301 hit 3.50.50.603 hits
InterProⁱ	View protein in InterPro IPR036188 FAD/NAD-bd_sf IPR023753 FAD/NAD-binding_dom IPR016156 FAD/NAD-linked_Rdtase_dimer_sf IPR001100 Pyr_nuc-diS_OxRdtase IPR004099 Pyr_nucl-diS_OxRdtase_dimer IPR012999 Pyr_OxRdtase_I_AS IPR006338 Thioredoxin/glutathione_Rdtase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07992 Pyr_redox_2, 1 hit PF02852 Pyr_redox_dim, 1 hit
PIRSFⁱ	PIRSF000350 Mercury_reductase_MerA, 1 hit
SUPFAMⁱ	SSF51905 SSF51905, 1 hit SSF55424 SSF55424, 1 hit
TIGRFAMsⁱ	TIGR01438 TGR, 1 hit
PROSITEⁱ	View protein in PROSITE PS00076 PYRIDINE_REDOX_1, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9VNT5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSTIKFLRSS THNALRSSLG WCRLAASRPR YDYDLVVLGG GSAGLACAKE 
        60         70         80         90        100
AAGCGARVLC FDYVKPTPVG TKWGIGGTCV NVGCIPKKLM HQASLLGEAV 
       110        120        130        140        150
HEAVAYGWNV DDTNIRPDWR KLVRSVQNHI KSVNWVTRVD LRDKKVEYVN 
       160        170        180        190        200
SMATFRDSHT IEYVAMPGAE HRQVTSEYVV VAVGGRPRYP DIPGAVELGI 
       210        220        230        240        250
TSDDIFSYER EPGRTLVVGA GYVGLECACF LKGLGYEPTV MVRSIVLRGF 
       260        270        280        290        300
DRQMSELLAA MMTERGIPFL GTTIPKAVER QADGRLLVRY RNTTTQMDGS 
       310        320        330        340        350
DVFDTVLWAI GRKGLIEDLN LDAAGVKTHD DKIVVDAAEA TSVPHIFAVG 
       360        370        380        390        400
DIIYGRPELT PVAILSGRLL ARRLFAGSTQ LMDYADVATT VFTPLEYSCV 
       410        420        430        440        450
GMSEETAIEL RGADNIEVFH GYYKPTEFFI PQKSVRHCYL KAVAEVSGDQ 
       460        470        480        490        500
KILGLHYIGP VAGEVIQGFA AALKTGLTVK TLLNTVGIHP TTAEEFTRLS 
       510 
ITKRSGRDPT PASCCS

Length:516

Mass (Da):56,277

Last modified:May 1, 2000 - v1

Checksum:ⁱD82A89CBF6A4B7E8

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF236866 Genomic DNA Translation: AAF64152.1 AE014296 Genomic DNA Translation: AAF51835.1 AY121613 mRNA Translation: AAM51940.1
NCBI Reference Sequences More...RefSeqⁱ	NP_524216.1, NM_079492.3
UniGeneⁱ	Dm.2498

Genome annotation databases

EnsemblMetazoaⁱ	FBtr0078514; FBpp0078166; FBgn0037170
GeneIDⁱ	40475
KEGGⁱ	dme:Dmel_CG11401

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9VNT5	UPI0007E800CF UPI0007E783F5 B4IB14 B4IUP2 A0A0R1EBM9 A0A0J9RZU9 UPI0007E69B62 UPI0007E7A2EF UPI0007E81174 UPI0007E44EC7	Drosophila rhopaloa (Fruit fly) Drosophila elegans (Fruit fly) Drosophila sechellia (Fruit fly) Drosophila yakuba (Fruit fly) Drosophila simulans (Fruit fly) Drosophila takahashii (Fruit fly) Drosophila biarmipes (Fruit fly) Drosophila eugracilis (Fruit fly) Drosophila suzukii (Spotted-wing drosophila fruit fly)	516	UniRef90_Q9VNT5	Cluster: Thioredoxin reductase 2, mitochondrial	11

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9VNT5	Q9JLT4-4 UPI00001BE54F A0A0U1RPV8 J3QMN4 UPI00084778CA A0A0M4F9W1 UPI00083ED0A7 UPI00083F2A8C A0A0Q9WGQ4 A0A0Q9WYT0 +240	Mus musculus (Mouse) Drosophila navojoa (Fruit fly) Drosophila busckii (Fruit fly) Drosophila virilis (Fruit fly) Drosophila mojavensis (Fruit fly) Drosophila hydei (Fruit fly) Drosophila arizonae (Fruit fly) Drosophila willistoni (Fruit fly) Rhincodon typus (whale shark) Bombyx mori (Silk moth) And more	493	UniRef50_Q9JLT4-4	Cluster: Isoform 4 of Thioredoxin reductase 2, mitochondrial	251

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF236866 Genomic DNA Translation: AAF64152.1 AE014296 Genomic DNA Translation: AAF51835.1 AY121613 mRNA Translation: AAM51940.1
NCBI Reference Sequences More...RefSeqⁱ	NP_524216.1, NM_079492.3
UniGeneⁱ	Dm.2498

3D structure databases

ProteinModelPortalⁱ	Q9VNT5
SMRⁱ	Q9VNT5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	657092 interactors.
Database of interacting proteins More...DIPⁱ	DIP-19796N
IntActⁱ	Q9VNT5 1 interactor.
STRINGⁱ	7227.FBpp0078166

Proteomic databases

PaxDbⁱ	Q9VNT5
PRIDEⁱ	Q9VNT5

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblMetazoaⁱ	FBtr0078514; FBpp0078166; FBgn0037170
GeneIDⁱ	40475
KEGGⁱ	dme:Dmel_CG11401

Organism-specific databases

CTDⁱ	40475
Drosophila genome database More...FlyBaseⁱ	FBgn0037170 Trxr-2

Phylogenomic databases

eggNOGⁱ	KOG0405 Eukaryota COG1249 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000007578
InParanoidⁱ	Q9VNT5
KEGG Orthology (KO) More...KOⁱ	K22182
OMAⁱ	GLECACF
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03IU
PhylomeDBⁱ	Q9VNT5

Enzyme and pathway databases

Reactomeⁱ	R-DME-3299685 Detoxification of Reactive Oxygen Species

Reactomeⁱ

R-DME-3299685 Detoxification of Reactive Oxygen Species

Miscellaneous databases

ChiTaRSⁱ	Trxr-2 fly
GenomeRNAiⁱ	40475
Protein Ontology More...PROⁱ	PR:Q9VNT5

Gene expression databases

Bgeeⁱ	FBgn0037170
Genevisibleⁱ	Q9VNT5 DM

Family and domain databases

Gene3Dⁱ	3.30.390.301 hit 3.50.50.603 hits
InterProⁱ	View protein in InterPro IPR036188 FAD/NAD-bd_sf IPR023753 FAD/NAD-binding_dom IPR016156 FAD/NAD-linked_Rdtase_dimer_sf IPR001100 Pyr_nuc-diS_OxRdtase IPR004099 Pyr_nucl-diS_OxRdtase_dimer IPR012999 Pyr_OxRdtase_I_AS IPR006338 Thioredoxin/glutathione_Rdtase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07992 Pyr_redox_2, 1 hit PF02852 Pyr_redox_dim, 1 hit
PIRSFⁱ	PIRSF000350 Mercury_reductase_MerA, 1 hit
SUPFAMⁱ	SSF51905 SSF51905, 1 hit SSF55424 SSF55424, 1 hit
TIGRFAMsⁱ	TIGR01438 TGR, 1 hit
PROSITEⁱ	View protein in PROSITE PS00076 PYRIDINE_REDOX_1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TRXR2_DROME
Accessionⁱ	Q9VNT5Primary (citable) accession number: Q9VNT5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
	Last sequence update:	May 1, 2000
	Last modified:	February 28, 2018
	This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Drosophila annotation project

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

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Annotation systems

Supporting data

UniProtKB - Q9VNT5 (TRXR2_DROME)

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Thioredoxin reductase 2, mitochondrial

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Mitochondrion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ