Reviewed, UniProtKB/Swiss-Prot Q9VNJ5 (DISP_DROME)

Names and origin

Protein names

Recommended name:
    Protein dispatched

Gene names

Name:	disp
ORF Names:	CG2019

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Segment polarity protein which functions in hedgehog (Hh) signaling. Regulates the trafficking and the release of cholesterol-modified hedgehog protein from cells of the posterior compartment (P cells) and is hence required for the effective production of the Hh signal. Ref.1 Ref.6 Ref.7
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Developmental stage	Ubiquitously expressed throughout the embryo and the imaginal discs. Ref.1
Disruption phenotype	Death during embryogenesis with a strong segment-polarity phenotype. Ref.1
Sequence similarities	Belongs to the dispatched family. Contains 1 SSD (sterol-sensing) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	exocytosis Traceable author statement. Source: FlyBase multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW pole cell migration Inferred from genetic interaction. Source: FlyBase smoothened signaling pathway Ref.1 Ref.6 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hedgehog receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1218	1218	Protein dispatched		PRO_0000310699
Regions
	Transmembrane	21 – 41	21	Potential
	Transmembrane	443 – 463	21	Potential
	Transmembrane	473 – 493	21	Potential
	Transmembrane	504 – 524	21	Potential
	Transmembrane	570 – 590	21	Potential
	Transmembrane	598 – 618	21	Potential
	Transmembrane	670 – 690	21	Potential
	Transmembrane	975 – 995	21	Potential
	Transmembrane	996 – 1016	21	Potential
	Transmembrane	1019 – 1039	21	Potential
	Transmembrane	1058 – 1078	21	Potential
	Transmembrane	1087 – 1107	21	Potential
	Domain	430 – 624	195	SSD
Amino acid modifications
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	264	1	N-linked (GlcNAc...) Potential
	Glycosylation	319	1	N-linked (GlcNAc...) Potential
	Glycosylation	388	1	N-linked (GlcNAc...) Ref.8
	Glycosylation	767	1	N-linked (GlcNAc...) Potential
	Glycosylation	883	1	N-linked (GlcNAc...) Potential
	Glycosylation	891	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	516 – 517	2	DD → AA: Loss of function; when associated with A-1030.
	Mutagenesis	516 – 517	2	DD → NN: Loss of function; when associated with N-1030.
	Mutagenesis	1030	1	D → A: Loss of function; when associated with 516-A-A-517. Ref.6
	Mutagenesis	1030	1	D → N: Loss of function; when associated with 517-N-N-517. Ref.6
	Sequence conflict	727	1	L → W in AAF23397. Ref.1
	Sequence conflict	779	1	D → G in AAV36904. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9VNJ5-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8214042F61156B63	FASTA	1,218	138,979
	10 20 30 40 50 60 MLCFDSERMN WYYHVLARRP YLVVVSIAVY CVACIIVALV LNKLPDFSDP TLGFETRGTK 70 80 90 100 110 120 IGERLTAWYN LLQETDHHGA LFSNPSDLWE RRRVEQGYVE TKLHPNHRRR KNKHKNRNKN 130 140 150 160 170 180 KRRKEQNQSS HEHHDVAQKM MQFKKRLKAT SSPSPNLGFD TWIGDSGVFR DYEITNDSAS 190 200 210 220 230 240 SSLEPTRRTE QIEYGHNTTS VDEEEHQQRV QTKKSTWRLL KQAATLPTDG WADMHRRQPI 250 260 270 280 290 300 EGFFCDSSPR KEYSHFVVQR IGPNATDSLF DLNGLLAMCQ LQDQITEVPS YRAFCEPEML 310 320 330 340 350 360 TTECCRPWSL PNYAAMLANK SSCFDLTTED VTSLHTLLLG CYEYFHDLKM DNHCNEIPHC 370 380 390 400 410 420 RAPEECKRLN IVFNVLNFLT DFSFIKSNDS NVYLKYAMIF IPVAQSNRLL PLFHEWEDVE 430 440 450 460 470 480 LINELVEVVA MDLGLENELF NELLLTDVWL VSLGGTFVMA SVWLYTGSAF ITLMSCVAIC 490 500 510 520 530 540 FSLGLAYFFY AIVLEFEFFP YMNLLAVVVI IGIGADDVFL FLKIWHCVLT ERFSNRCTLT 550 560 570 580 590 600 TQSQSALPTL ENSDHTESLE NIMALTMRHA AASMFVTSLT TAGAFYASYS SSITAIKCFG 610 620 630 640 650 660 IFAGTVVVTN YLLMITWLPA SVSIMERLFA TRMSCHHPMS IKLIHACKKS INRFCQMFEE 670 680 690 700 710 720 CITKSIMNYA YLWLLIFGAL GASSAVIVFW YPGLQLPEKS HFQLFVSKHP FEVYSSLKQQ 730 740 750 760 770 780 FWFEKPLQAY ENFKMHMHFV WGVQAVDDGD YTNPNSYGHL HYDNNFNVSS RPAQLWILDF 790 800 810 820 830 840 CQSVRQQPFY KETLGMLLPN CFIENLIDYM KRRCIDDMDS TRKDRSPCCD AQFPFEPHIF 850 860 870 880 890 900 EYCLPQSISN MYDTTFFRPG VAGPKFAEAP RLETEDYLGM SGNESAEYST NGSFTPLLVK 910 920 930 940 950 960 ALVIEFESNV AYSTIYANIR QFYESVEHWF QMQLKTAPPE LQGGWFTSDL KFYNVQDTLS 970 980 990 1000 1010 1020 HDTFVAICLA MAASLAVLLC FTVNILISIY AVLTVSLSIF NTVAVLILLG WQLNILESIA 1030 1040 1050 1060 1070 1080 VSTAIGLAVD FSLHYGIHYR MSPVKERLAA TQFVLSRIIG PTVMAATTTG LAGGIMMASN 1090 1100 1110 1120 1130 1140 ILPYIQIGVF LVVVMIVSWF YATFFLMSLL RVAGPQHGFL ELKWPLWSKR SSGSSKFYER 1150 1160 1170 1180 1190 1200 KPSQVIASEQ LLTPTSSAIV ELANSETHEL ESLNSNSLIK TISGIESAHA LSSLPRDFEH 1210 SFQTMHECKY QTYPSTSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dispatched, a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells." Burke R., Nellen D., Bellotto M., Hafen E., Senti K.-A., Dickson B.J., Basler K. Cell 99:803-815(1999) [PubMed: 10619433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
[6]	"Hedgehog-mediated patterning of the mammalian embryo requires transporter-like function of dispatched." Ma Y., Erkner A., Gong R., Yao S., Taipale J., Basler K., Beachy P.A. Cell 111:63-75(2002) [PubMed: 12372301] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF 516-ASP-ASP-517 AND ASP-1030.
[7]	"Cholesterol modification of hedgehog is required for trafficking and movement, revealing an asymmetric cellular response to hedgehog." Gallet A., Rodriguez R., Ruel L., Therond P.P. Dev. Cell 4:191-204(2003) [PubMed: 12586063] [Abstract] Cited for: FUNCTION.
[8]	"Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster." Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V. Glycobiology 17:1388-1403(2007) [PubMed: 17893096] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-388, MASS SPECTROMETRY. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF200691 mRNA. Translation: AAF23397.1. AE014297 Genomic DNA. Translation: AAF51938.1. AY075394 mRNA. Translation: AAL68228.1. BT016019 mRNA. Translation: AAV36904.1. BT050429 mRNA. Translation: ACJ13136.1.
RefSeq	NP_524734.2.
UniGene	Dm.1845
3D structure databases
SMR	Q9VNJ5. Positions 449-627, 982-1114.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VNJ5. 1 interaction.
STRING	Q9VNJ5.
Proteomic databases
PRIDE	Q9VNJ5.
Genome annotation databases
Ensembl	FBtr0078665; FBpp0078314; FBgn0029088; Drosophila melanogaster. [Genome view]
GeneID	44274.
KEGG	dme:Dmel_CG2019.
NMPDR	fig|7227.3.peg.11321.
UCSC	CG2019-RA. d. melanogaster.
Organism-specific databases
CTD	44274.
FlyBase	FBgn0029088. disp.
Phylogenomic databases
eggNOG	inNOG07871.
InParanoid	Q9VNJ5.
OMA	PNCFIEN.
OrthoDB	EOG92FSQN.
PhylomeDB	Q9VNJ5.
Gene expression databases
Bgee	Q9VNJ5.
Family and domain databases
InterPro	IPR003392. Patched. IPR000731. SSD_5TM. [Graphical view]
Pfam	PF02460. Patched. 1 hit. [Graphical view]
PROSITE	PS50156. SSD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	837097.

Entry information

Entry name

DISP_DROME

Accession

Primary (citable) accession number: Q9VNJ5
Secondary accession number(s): B6IDI9 , Q5U173, Q8SY40, Q9U477

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project