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Protein

Odorant receptor coreceptor

Gene

Orco

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Odorant coreceptor which complexes with conventional odorant receptors (ORs) to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. Orco is a universal and integral part of the functional odorant receptor, involved in the dendritic localization of other olfactory receptors. Expression of Orco alone leads to formation of rapid and transient ion channels not directly responding to odorants, but directly activated by intracellular cAMP or cGMP. Snmp, Or67d and lush act in concert to capture fatty-acid-derived male pheromone 11-cis vaccenyl acetate (cVA) molecules on the surface of Or67d expressing olfactory dendrites and facilitate their transfer to the odorant-receptor Orco complex.9 Publications

Miscellaneous

The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Orco complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways. The homomeric Orco channel is thought to be a cyclic-nucleotide-gated ion channel that depolarizes the olfactory receptor neuron.

GO - Molecular functioni

  • calcium-release channel activity Source: FlyBase
  • calmodulin binding Source: FlyBase
  • odorant binding Source: FlyBase
  • olfactory receptor activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • behavioral response to ethanol Source: FlyBase
  • detection of chemical stimulus involved in sensory perception of smell Source: FlyBase
  • olfactory behavior Source: FlyBase
  • protein heterooligomerization Source: FlyBase
  • regulation of protein localization Source: FlyBase
  • response to pheromone Source: FlyBase
  • sensory perception of smell Source: FlyBase

Keywordsi

Molecular functionReceptor, Transducer
Biological processBehavior, Olfaction, Sensory transduction

Protein family/group databases

TCDBi1.A.69.1.1. the heteromeric odorant receptor channel (horc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Odorant receptor coreceptor
Alternative name(s):
Odorant receptor 83b
Gene namesi
Name:Orco
Synonyms:A45, Or83b
ORF Names:CG10609
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037324. Orco.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 47Cytoplasmic1 PublicationAdd BLAST47
Transmembranei48 – 68Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini69 – 75Extracellular1 Publication7
Transmembranei76 – 96Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini97 – 135Cytoplasmic1 PublicationAdd BLAST39
Transmembranei136 – 156Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini157 – 191Extracellular1 PublicationAdd BLAST35
Transmembranei192 – 212Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini213 – 351Cytoplasmic1 PublicationAdd BLAST139
Transmembranei352 – 372Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini373 – 390Extracellular1 PublicationAdd BLAST18
Transmembranei391 – 411Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini412 – 462Cytoplasmic1 PublicationAdd BLAST51
Transmembranei463 – 483Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini484 – 486Extracellular1 Publication3

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi169N → NQ: Destroys glycosylation site. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001742721 – 486Odorant receptor coreceptorAdd BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi169N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi188N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9VNB5.
PRIDEiQ9VNB5.

PTM databases

iPTMnetiQ9VNB5.

Expressioni

Tissue specificityi

Expression is restricted to olfactory sensory neurons (OSNs). Coexpressed with Snmp in a lateral-distal population of OSNs. Expressed in the embryonic antennal-maxillary complex, in all 21 OSNs of the larval dorsal organ, in the pupal antennal OSNs, in all 120 adult maxillary palp neurons and in approximately 70-80% of adult antennal OSNs, where expression is highest at the dorsal-medial edge. Localized to OSN cell bodies and to the distal portion of ciliated OSN dendrites.5 Publications

Developmental stagei

Expressed throughout all developmental stages. First expressed at embryonic stage 15. Pupal expression first occurs 80 hours after puparium formation.1 Publication

Gene expression databases

BgeeiFBgn0037324.
ExpressionAtlasiQ9VNB5. differential.
GenevisibleiQ9VNB5. DM.

Interactioni

Subunit structurei

Heterodimer with conventional odorant receptors (ORs). Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway.

Binary interactionsi

WithEntry#Exp.IntActNotes
Or43aP819172EBI-15562228,EBI-15562282

GO - Molecular functioni

  • calmodulin binding Source: FlyBase
  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi65859. 5 interactors.
DIPiDIP-29174N.
IntActiQ9VNB5. 2 interactors.
STRINGi7227.FBpp0112105.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IK8M. Eukaryota.
ENOG4110PZW. LUCA.
InParanoidiQ9VNB5.
KOiK08471.
OMAiQIYYVLF.
OrthoDBiEOG091G07IH.
PhylomeDBiQ9VNB5.

Family and domain databases

InterProiView protein in InterPro
IPR004117. 7tm6_olfct_rcpt.
PANTHERiPTHR21137. PTHR21137. 1 hit.
PfamiView protein in Pfam
PF02949. 7tm_6. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VNB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSMQPSKY TGLVADLMPN IRAMKYSGLF MHNFTGGSAF MKKVYSSVHL
60 70 80 90 100
VFLLMQFTFI LVNMALNAEE VNELSGNTIT TLFFTHCITK FIYLAVNQKN
110 120 130 140 150
FYRTLNIWNQ VNTHPLFAES DARYHSIALA KMRKLFFLVM LTTVASATAW
160 170 180 190 200
TTITFFGDSV KMVVDHETNS SIPVEIPRLP IKSFYPWNAS HGMFYMISFA
210 220 230 240 250
FQIYYVLFSM IHSNLCDVMF CSWLIFACEQ LQHLKGIMKP LMELSASLDT
260 270 280 290 300
YRPNSAALFR SLSANSKSEL IHNEEKDPGT DMDMSGIYSS KADWGAQFRA
310 320 330 340 350
PSTLQSFGGN GGGGNGLVNG ANPNGLTKKQ EMMVRSAIKY WVERHKHVVR
360 370 380 390 400
LVAAIGDTYG AALLLHMLTS TIKLTLLAYQ ATKINGVNVY AFTVVGYLGY
410 420 430 440 450
ALAQVFHFCI FGNRLIEESS SVMEAAYSCH WYDGSEEAKT FVQIVCQQCQ
460 470 480
KAMSISGAKF FTVSLDLFAS VLGAVVTYFM VLVQLK
Length:486
Mass (Da):54,413
Last modified:June 1, 2001 - v2
Checksum:i8DD0ACDC3091B5D6
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti143T → I in strain: Oregon-R. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY567998 mRNA. Translation: AAT71306.1.
AE014297 Genomic DNA. Translation: AAF52031.2.
AE014297 Genomic DNA. Translation: ABW08603.1.
RefSeqiNP_001097687.1. NM_001104217.2.
NP_524235.2. NM_079511.5.
UniGeneiDm.25037.

Genome annotation databases

EnsemblMetazoaiFBtr0078794; FBpp0078438; FBgn0037324.
FBtr0113193; FBpp0112105; FBgn0037324.
GeneIDi40650.
KEGGidme:Dmel_CG10609.
UCSCiCG10609-RB. d. melanogaster.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiORCO_DROME
AccessioniPrimary (citable) accession number: Q9VNB5
Secondary accession number(s): A8JQT8, Q672R0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 2001
Last modified: October 25, 2017
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families