ID   7B2_DROME               Reviewed;         276 AA.
AC   Q59E04; E4NKI5; Q9N9Z7; Q9VNB0;
DT   22-FEB-2023, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   24-JAN-2024, entry version 118.
DE   RecName: Full=Neuroendocrine protein 7B2 {ECO:0000303|PubMed:10749852};
DE   Flags: Precursor;
GN   Name=7B2 {ECO:0000303|PubMed:10749852,
GN   ECO:0000312|FlyBase:FBgn0041707};
GN   ORFNames=CG1168 {ECO:0000312|FlyBase:FBgn0041707};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN   [1] {ECO:0000312|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2] {ECO:0000312|Proteomes:UP000000803}
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3] {ECO:0000312|EMBL:AAM50107.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC   STRAIN=Berkeley {ECO:0000312|EMBL:AAM50107.1};
RC   TISSUE=Head {ECO:0000312|EMBL:AAM50107.1};
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [4] {ECO:0000312|EMBL:ADQ89796.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC   STRAIN=Berkeley {ECO:0000312|EMBL:ADQ89796.1};
RC   TISSUE=Head {ECO:0000312|EMBL:ADQ89796.1};
RA   Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL   Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-276, AND FUNCTION.
RX   PubMed=10749852; DOI=10.1074/jbc.m000032200;
RA   Hwang J.R., Siekhaus D.E., Fuller R.S., Taghert P.H., Lindberg I.;
RT   "Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2.
RT   Insect cell-specific processing and secretion.";
RL   J. Biol. Chem. 275:17886-17893(2000).
CC   -!- FUNCTION: Acts as a molecular chaperone for neuroendocrine convertase
CC       amon/PC2, preventing its premature activation in the regulated
CC       secretory pathway (PubMed:10749852). Binds to inactive amon in the
CC       endoplasmic reticulum and facilitates its transport from there to later
CC       compartments of the secretory pathway where it is proteolytically
CC       matured and activated (By similarity). Also required for cleavage of
CC       amon (PubMed:10749852). {ECO:0000250|UniProtKB:P12961,
CC       ECO:0000269|PubMed:10749852}.
CC   -!- SUBUNIT: Interacts with amon/PC2 early in the secretory pathway.
CC       Dissociation occurs at later stages. {ECO:0000250|UniProtKB:P05408}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P01165}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=B {ECO:0000312|FlyBase:FBgn0041707};
CC         IsoId=Q59E04-1; Sequence=Displayed;
CC       Name=A {ECO:0000312|FlyBase:FBgn0041707};
CC         IsoId=Q59E04-2; Sequence=VSP_061793;
CC   -!- SIMILARITY: Belongs to the 7B2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ADQ89796.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE014297; AAX52936.1; -; Genomic_DNA.
DR   EMBL; AE014297; AAF52036.3; -; Genomic_DNA.
DR   EMBL; AY119453; AAM50107.1; -; mRNA.
DR   EMBL; BT125782; ADQ89796.1; ALT_INIT; mRNA.
DR   EMBL; AJ271974; CAB72934.1; -; Genomic_DNA.
DR   RefSeq; NP_001014608.1; NM_001014608.2. [Q59E04-1]
DR   RefSeq; NP_620471.3; NM_138986.4. [Q59E04-2]
DR   AlphaFoldDB; Q59E04; -.
DR   IntAct; Q59E04; 2.
DR   STRING; 7227.FBpp0100150; -.
DR   MEROPS; I21.001; -.
DR   PaxDb; 7227-FBpp0100150; -.
DR   DNASU; 40644; -.
DR   EnsemblMetazoa; FBtr0078789; FBpp0078435; FBgn0041707. [Q59E04-2]
DR   EnsemblMetazoa; FBtr0301640; FBpp0100150; FBgn0041707. [Q59E04-1]
DR   GeneID; 40644; -.
DR   KEGG; dme:Dmel_CG1168; -.
DR   UCSC; CG1168-RB; d. melanogaster. [Q59E04-1]
DR   AGR; FB:FBgn0041707; -.
DR   CTD; 40644; -.
DR   FlyBase; FBgn0041707; 7B2.
DR   VEuPathDB; VectorBase:FBgn0041707; -.
DR   eggNOG; KOG4187; Eukaryota.
DR   GeneTree; ENSGT00390000009816; -.
DR   HOGENOM; CLU_080823_0_0_1; -.
DR   InParanoid; Q59E04; -.
DR   OMA; CTCDSEH; -.
DR   OrthoDB; 3024399at2759; -.
DR   PhylomeDB; Q59E04; -.
DR   BioGRID-ORCS; 40644; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 40644; -.
DR   Proteomes; UP000000803; Chromosome 3R.
DR   Bgee; FBgn0041707; Expressed in head capsule and 13 other cell types or tissues.
DR   ExpressionAtlas; Q59E04; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030141; C:secretory granule; IEA:InterPro.
DR   GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR   GO; GO:0016504; F:peptidase activator activity; IDA:FlyBase.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:InterPro.
DR   GO; GO:0045862; P:positive regulation of proteolysis; IDA:FlyBase.
DR   GO; GO:0046883; P:regulation of hormone secretion; IBA:GO_Central.
DR   InterPro; IPR007945; Secretogranin_V.
DR   PANTHER; PTHR12738; NEUROENDOCRINE PROTEIN 7B2; 1.
DR   PANTHER; PTHR12738:SF0; NEUROENDOCRINE PROTEIN 7B2; 1.
DR   Pfam; PF05281; Secretogranin_V; 1.
DR   Genevisible; Q59E04; DM.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Chaperone; Disulfide bond; Reference proteome;
KW   Secreted; Signal; Transport.
FT   SIGNAL          1..?
FT                   /evidence="ECO:0000305"
FT   CHAIN           ?..276
FT                   /note="Neuroendocrine protein 7B2"
FT                   /id="PRO_0000457563"
FT   DISULFID        155..168
FT                   /evidence="ECO:0000250|UniProtKB:P18844"
FT   VAR_SEQ         7..12
FT                   /note="THVFPG -> S (in isoform A)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_061793"
SQ   SEQUENCE   276 AA;  29995 MW;  DDCE6CA4728F7E41 CRC64;
     MLFRSQTHVF PGVYMMVAML VVALSGYQVQ SYSAKDILAD VLMTDLLNRM DKDMQVGYYD
     VGNEAAAGSK DNVDLVSRSE YARLCDGGSD CILQSGSASG AASHPSLRDD EFLQHSSLWG
     HQFISGGMGE GPNRYPTIVK NDAGLPAYCN PPNPCPEGYD METQGGSCIV DFENTAIFSR
     EFQAAQDCTC DNEHMFDCSE QDSADVGGDK GDLNSAVEQY IMQMGQENSL NNVNSLAKKA
     GYPVMPDPRL DDAVINPFLQ GDRLPIAAKK GNLLFH
//