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Protein

Proteasome subunit beta type-4

Gene

Prosbeta7

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: FlyBase
  • centrosome cycle Source: FlyBase
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-209360. Ubiquitination and proteolysis of phosphorylated CI.
R-DME-209406. Degradation of NF-kappa-B inhibitor, CACT.
R-DME-209461. Ubiquitination and degradation of phosphorylated ARM.
R-DME-216167. Nuclear CI is degraded.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-432395. Degradation of TIM.
R-DME-432524. Degradation of PER.
R-DME-446652. Interleukin-1 family signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-538848. Degradation of CLK.
R-DME-538864. Degradation of CRY.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-8948751. Regulation of PTEN stability and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-4 (EC:3.4.25.1)
Alternative name(s):
Proteasome subunit beta type-7
Gene namesi
Name:Prosbeta7
Synonyms:Prosb4, Prosbeta4
ORF Names:CG12000
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0250746. Prosbeta7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001480711 – 268Proteasome subunit beta type-4Add BLAST268

Proteomic databases

PaxDbiQ9VNA5.
PRIDEiQ9VNA5.

Expressioni

Gene expression databases

BgeeiFBgn0250746.
GenevisibleiQ9VNA5. DM.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).By similarity

Protein-protein interaction databases

BioGridi65850. 42 interactors.
DIPiDIP-19164N.
IntActiQ9VNA5. 8 interactors.
MINTiMINT-924269.
STRINGi7227.FBpp0078448.

Structurei

3D structure databases

ProteinModelPortaliQ9VNA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0185. Eukaryota.
ENOG410YAMA. LUCA.
GeneTreeiENSGT00390000000698.
InParanoidiQ9VNA5.
KOiK02736.
OMAiKECMKVL.
OrthoDBiEOG091G0JV7.
PhylomeDBiQ9VNA5.

Family and domain databases

CDDicd03760. proteasome_beta_type_4. 1 hit.
Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR016295. Proteasome_endopept_cplx_B.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
PANTHERiPTHR11599:SF5. PTHR11599:SF5. 1 hit.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PIRSFiPIRSF001213. Psome_endopept_beta. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VNA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNNYNSLAQ PMWQNGPAPG EFYNFTGGQT PVQQLPRELT TMGPYGTKHS
60 70 80 90 100
TASSTTGTSV LGIRYDSGVM LAADTLVSYG SMARYQNIER VFKVNKNILL
110 120 130 140 150
GGSGDFADIQ SIKRNIDQKM IEDQCCDDNI EMKPKSLASW MTRVLYNRRS
160 170 180 190 200
RMNPLYIDVV VGGVDNEGTP YLANVDLRGR SYEDYVVATG FARHLAVPLV
210 220 230 240 250
REKKPKDRDF TAVEASELIR TCMEVLYYRD TRNISQYTVG VCSVNGCGVE
260
GPFQVNENWT FAETIKGY
Length:268
Mass (Da):29,986
Last modified:May 1, 2000 - v1
Checksum:i215433952659EAD0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF52041.1.
AE014297 Genomic DNA. Translation: AAN13277.1.
AY118936 mRNA. Translation: AAM50796.1.
RefSeqiNP_649529.1. NM_141272.1.
NP_730922.1. NM_169049.2.
UniGeneiDm.11642.

Genome annotation databases

EnsemblMetazoaiFBtr0078805; FBpp0078448; FBgn0250746.
FBtr0078806; FBpp0078449; FBgn0250746.
GeneIDi40639.
KEGGidme:Dmel_CG12000.

Similar proteinsi

Entry informationi

Entry nameiPSB4_DROME
AccessioniPrimary (citable) accession number: Q9VNA5
Secondary accession number(s): A4V2F4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 2000
Last modified: November 22, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families