Q9VNA5 (PSB4_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit beta type-4
EC=3.4.25.1

Alternative name(s):
Proteasome subunit beta type-7

Gene names

Name:	Prosbeta7
Synonyms:	Prosb4, Prosbeta4
ORF Names:	CG12000

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity By similarity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1B family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell proliferation Inferred from mutant phenotype Ref.4. Source: FlyBase centrosome organization Inferred from mutant phenotype Ref.4. Source: FlyBase mitotic spindle elongation Inferred from mutant phenotype Ref.4. Source: FlyBase proteolysis involved in cellular protein catabolic process Inferred from electronic annotation. Source: InterPro response to DNA damage stimulus Inferred from mutant phenotype. Source: FlyBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Proteasome subunit beta type-4		PRO_0000148071

Sequences

	Sequence		Length	Mass (Da)
	Q9VNA5 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 215433952659EAD0	FASTA	268	29,986
	10 20 30 40 50 60 MLNNYNSLAQ PMWQNGPAPG EFYNFTGGQT PVQQLPRELT TMGPYGTKHS TASSTTGTSV 70 80 90 100 110 120 LGIRYDSGVM LAADTLVSYG SMARYQNIER VFKVNKNILL GGSGDFADIQ SIKRNIDQKM 130 140 150 160 170 180 IEDQCCDDNI EMKPKSLASW MTRVLYNRRS RMNPLYIDVV VGGVDNEGTP YLANVDLRGR 190 200 210 220 230 240 SYEDYVVATG FARHLAVPLV REKKPKDRDF TAVEASELIR TCMEVLYYRD TRNISQYTVG 250 260 VCSVNGCGVE GPFQVNENWT FAETIKGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	"Genes required for mitotic spindle assembly in Drosophila S2 cells." Goshima G., Wollman R., Goodwin S.S., Zhang N., Scholey J.M., Vale R.D., Stuurman N. Science 316:417-421(2007) [PubMed: 17412918] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: AAF52041.1. AE014297 Genomic DNA. Translation: AAN13277.1. AY118936 mRNA. Translation: AAM50796.1.
RefSeq	NP_649529.1. NM_141272.1. NP_730922.1. NM_169049.1.
UniGene	Dm.11642.
3D structure databases
ProteinModelPortal	Q9VNA5.
SMR	Q9VNA5. Positions 50-267.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19164N.
IntAct	Q9VNA5. 8 interactions.
MINT	MINT-924269.
STRING	Q9VNA5.
Protein family/group databases
MEROPS	T01.987.
Proteomic databases
PRIDE	Q9VNA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0078805; FBpp0078448; FBgn0250746. FBtr0078806; FBpp0078449; FBgn0250746.
GeneID	40639.
KEGG	dme:Dmel_CG12000.
Organism-specific databases
CTD	40639.
FlyBase	FBgn0250746. Prosbeta7.
Phylogenomic databases
eggNOG	inNOG06356.
GeneTree	EMGT00050000009439.
InParanoid	Q9VNA5.
OMA	RIMRVND.
OrthoDB	EOG4Q575B.
PhylomeDB	Q9VNA5.
Gene expression databases
Bgee	Q9VNA5.
GermOnline	CG12000. Drosophila melanogaster.
Family and domain databases
InterPro	IPR016050. Proteasome_bsu_CS. IPR016295. Proteasome_endopept_cplx_B. IPR001353. Proteasome_sua/b. IPR023333. Proteasome_suB-type. [Graphical view]
KO	K02736.
Pfam	PF00227. Proteasome. 1 hit. [Graphical view]
PIRSF	PIRSF001213. Psome_endopept_beta. 1 hit.
PROSITE	PS00854. PROTEASOME_B_1. 1 hit. PS51476. PROTEASOME_B_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	819792.

Entry information

Entry name

PSB4_DROME

Accession

Primary (citable) accession number: Q9VNA5
Secondary accession number(s): A4V2F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project