Reviewed, UniProtKB/Swiss-Prot Q9VMT6 (C28D2_DROME)

Names and origin

Protein names

Recommended name:
    Probable cytochrome P450 28d2
    EC=1.14.-.-
Alternative name(s):
    CYPXXVIIID2

Gene names

Name:	Cyp28d2
ORF Names:	CG6081

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein Potential. Microsome membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence AAF52225.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VWZ8	1	EBI-156554,EBI-94666

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Probable cytochrome P450 28d2		PRO_0000051991
Sites
	Metal binding	446	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9VMT6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 9AD85F249390A655	FASTA	501	58,222
	10 20 30 40 50 60 MCPVTTFLVL VLTLLVLVYV FLTWNFNYWR KRGIKTAPTW PFVGSFPSIF TRKRNIAYDI 70 80 90 100 110 120 DDIYEKYKDT DNMVGVFTTR VPQLLVMCPE YIHKIYATDF RSFHNNEWRN FVNKKTDMIL 130 140 150 160 170 180 GNNPFVLTGD EWKERRSEIM PALSPNRVKA VYPVSQSVCK KFVEYIRRQQ QMATSEGLDA 190 200 210 220 230 240 MDLSLCYTTE VVSDCGLGVS AQSFTDTPTP LLKMIKRVFN TSFEFIFYSV VTNLWQKVRK 250 260 270 280 290 300 FYSVPFFNKE TEVFFLDIIR RCITLRLEKP EQQRDDFLNY MLQLQEKKGL HTDNILINTM 310 320 330 340 350 360 TFILDGFETT ALVLAHIMLM LGRNPEEQDK VRKEIGSADL TFDQMSELPH LDACIYETLR 370 380 390 400 410 420 LFSPQVAARK LVTEPFEFAN KNGRTVHLKP GDVVTIPVKA LHHDPQYYED PLTFKPERFL 430 440 450 460 470 480 ESNGGGMKSY RDRGVYLAFG DGPRHCPGMR FALTQLKAAL VEILRNFEIK VNPKTRSDNQ 490 500 IDDTFFMATL KGGIYLDFKD L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	Nelson B. Unpublished observations (SEP-2000) Cited for: CONCEPTUAL TRANSLATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014134 Genomic DNA. Translation: AAF52225.1. Sequence problems.
RefSeq	NP_608911.1.
UniGene	Dm.23848
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:20831N.
IntAct	Q9VMT6. 2 interactions.
Proteomic databases
PRIDE	Q9VMT6.
Genome annotation databases
Ensembl	FBgn0031688. Drosophila melanogaster. [Contig view]
GeneID	33748.
KEGG	dme:Dmel_CG6081.
NMPDR	fig|7227.3.peg.855.
Organism-specific databases
FlyBase	FBgn0031688. Cyp28d2.
Phylogenomic databases
HOGENOM	Q9VMT6.
OMA	Q9VMT6. ERRSEIM.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-007451-MON.
Gene expression databases
ArrayExpress	Q9VMT6.
GermOnline	CG6081. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	785052.

Entry information

Entry name

C28D2_DROME

Accession

Primary (citable) accession number: Q9VMT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project