Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

60 kDa heat shock protein homolog 2, mitochondrial

Gene

Hsp60C

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity). Essential for proper development of trachea, spermatogonia and spermatocytes.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • 'de novo' protein folding Source: FlyBase
  • female germline ring canal formation Source: FlyBase
  • oogenesis Source: FlyBase
  • open tracheal system development Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • protein refolding Source: InterPro
  • protein targeting to mitochondrion Source: FlyBase
  • spermatogenesis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Developmental protein

Keywords - Biological processi

Differentiation, Oogenesis, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
60 kDa heat shock protein homolog 2, mitochondrial
Alternative name(s):
60 kDa chaperonin
CPN60
Heat shock protein 60
Short name:
HSP-60
Hsp60
Gene namesi
Name:Hsp60C
ORF Names:CG7235
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0031728. Hsp60C.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Flies are male and female sterile; reduction in numbers of primary and secondary spermatocytes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6161MitochondrionBy similarityAdd
BLAST
Chaini62 – 57651560 kDa heat shock protein homolog 2, mitochondrialPRO_0000005033Add
BLAST

Proteomic databases

PaxDbiQ9VMN5.
PRIDEiQ9VMN5.

Expressioni

Tissue specificityi

First detectable expression is seen in the posterior part of the dorsal tracheal trunk at stage 14-15, which marks the beginning of terminal tracheation. In the larval gut, expression in proventriculus is stronger than in midgut and hindgut. Malpighian tubules shows low expression and late third instar larval imaginal disks and brain showed moderate expression. In larval ovary and testis, expression is strong in the posterior region.1 Publication

Developmental stagei

Expression first seen in late embryonic stages and continues in larval and adult stages.1 Publication

Gene expression databases

BgeeiQ9VMN5.
GenevisibleiQ9VMN5. DM.

Interactioni

Protein-protein interaction databases

BioGridi59964. 14 interactions.
DIPiDIP-20697N.
IntActiQ9VMN5. 14 interactions.
MINTiMINT-287822.
STRINGi7227.FBpp0078742.

Structurei

3D structure databases

ProteinModelPortaliQ9VMN5.
SMRiQ9VMN5. Positions 27-550.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chaperonin (HSP60) family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0356. Eukaryota.
COG0459. LUCA.
GeneTreeiENSGT00390000005727.
InParanoidiQ9VMN5.
KOiK04077.
OMAiGNMIERG.
OrthoDBiEOG7HTHGJ.
PhylomeDBiQ9VMN5.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.50.7.10. 1 hit.
HAMAPiMF_00600. CH60.
InterProiIPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00298. CHAPERONIN60.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02348. GroEL. 1 hit.
PROSITEiPS00296. CHAPERONINS_CPN60. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9VMN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRMFRYTNT LQRTAKISHV LWARNYAKDV RFGPEVRAMM LQGVDVLADA
60 70 80 90 100
VAVTMGPKGR NVIIEQSWGS PKITKDGVTV AKSIALKDKF QNIGAKLVQD
110 120 130 140 150
VANNTNEEAG DGTTTATVLA RAIAKEGFEK ISRGASPVEI RRGVMLAIET
160 170 180 190 200
VKDNLRRLSR PVNTPEEICQ VATISANGDK SVGNLISEAI KKVGRDGVIT
210 220 230 240 250
VKDGKTLCDE LEVIEGMKFD RGYISPYFIN TSKGAKVEFQ DALLLFCEKK
260 270 280 290 300
IKSAPSIVPA LELANAQRKP LVIIAEDLEA EALSTLVVNR LKVGLQVCAV
310 320 330 340 350
KAPGFGDNRK ENLMDMAVAT GGIVFGDEAN MVRLEDIKMS DFGRVGEVVV
360 370 380 390 400
SKDDTMLLKG KGQKAEVEKR VEGLREAIKE STSSYEKEKM QERLARLSSG
410 420 430 440 450
VALLRVGGSS DVEVSEKKDR VIDALNATRA AVEEGIVPGG GTALLRCIQK
460 470 480 490 500
LNDLKGANED QNMGIEIIRR ALRMPCLTIA KNAGVDGAMV VAKVEILDGD
510 520 530 540 550
YGYDALKGEY GNMIERGIID PTKVVRTAIS DAAGVASLLT TAEAVVTELP
560 570
LEEAAAAGAA AGLGALGGMG MGGMGM
Length:576
Mass (Da):61,587
Last modified:May 10, 2004 - v2
Checksum:i7FB52C049E9A5F79
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601R → Q in AAM29278 (PubMed:12537569).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF52277.2.
AE014134 Genomic DNA. Translation: AAN10550.2.
AE014134 Genomic DNA. Translation: AAN10551.2.
AY113273 mRNA. Translation: AAM29278.1.
BT001308 mRNA. Translation: AAN71063.1.
RefSeqiNP_608948.2. NM_135104.5.
NP_723104.2. NM_164654.2.
NP_723105.2. NM_164655.3.
UniGeneiDm.6406.

Genome annotation databases

EnsemblMetazoaiFBtr0079110; FBpp0078742; FBgn0031728.
FBtr0079111; FBpp0078743; FBgn0031728.
FBtr0079112; FBpp0078744; FBgn0031728.
GeneIDi33796.
KEGGidme:Dmel_CG7235.
UCSCiCG7235-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF52277.2.
AE014134 Genomic DNA. Translation: AAN10550.2.
AE014134 Genomic DNA. Translation: AAN10551.2.
AY113273 mRNA. Translation: AAM29278.1.
BT001308 mRNA. Translation: AAN71063.1.
RefSeqiNP_608948.2. NM_135104.5.
NP_723104.2. NM_164654.2.
NP_723105.2. NM_164655.3.
UniGeneiDm.6406.

3D structure databases

ProteinModelPortaliQ9VMN5.
SMRiQ9VMN5. Positions 27-550.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59964. 14 interactions.
DIPiDIP-20697N.
IntActiQ9VMN5. 14 interactions.
MINTiMINT-287822.
STRINGi7227.FBpp0078742.

Proteomic databases

PaxDbiQ9VMN5.
PRIDEiQ9VMN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079110; FBpp0078742; FBgn0031728.
FBtr0079111; FBpp0078743; FBgn0031728.
FBtr0079112; FBpp0078744; FBgn0031728.
GeneIDi33796.
KEGGidme:Dmel_CG7235.
UCSCiCG7235-RA. d. melanogaster.

Organism-specific databases

CTDi33796.
FlyBaseiFBgn0031728. Hsp60C.

Phylogenomic databases

eggNOGiKOG0356. Eukaryota.
COG0459. LUCA.
GeneTreeiENSGT00390000005727.
InParanoidiQ9VMN5.
KOiK04077.
OMAiGNMIERG.
OrthoDBiEOG7HTHGJ.
PhylomeDBiQ9VMN5.

Miscellaneous databases

ChiTaRSiHsp60C. fly.
GenomeRNAii33796.
PROiQ9VMN5.

Gene expression databases

BgeeiQ9VMN5.
GenevisibleiQ9VMN5. DM.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.50.7.10. 1 hit.
HAMAPiMF_00600. CH60.
InterProiIPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00298. CHAPERONIN60.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02348. GroEL. 1 hit.
PROSITEiPS00296. CHAPERONINS_CPN60. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Testis.
  4. "The Hsp60C gene in the 25F cytogenetic region in Drosophila melanogaster is essential for tracheal development and fertility."
    Sarkar S., Lakhotia S.C.
    J. Genet. 84:265-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCH60C_DROME
AccessioniPrimary (citable) accession number: Q9VMN5
Secondary accession number(s): A4V087, Q8IHD0, Q8MZB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 10, 2004
Last modified: June 8, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.