Q9VMA7 (TGO1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transport and Golgi organization protein 1

Gene names

Name:	Tango1
ORF Names:	CG11098

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for protein secretion. May participate in cargo loading by binding to COPII coat subunits and guiding SH3-bound proteins into a growing carrier. Ref.6
Subcellular location	Golgi apparatus membrane; Single-pass type I membrane protein Ref.6.
Sequence similarities	Belongs to the MIA/OTOR family. Tango1 subfamily. Contains 1 SH3 domain.
Sequence caution	The sequence AAL68225.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAL68225.1 differs from that shown. Reason: Deletion of 12 residues at position 1268 causing a frameshift.

Ontologies

Keywords
Biological process	ER-Golgi transport Exocytosis Protein transport Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Signal Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi organization Inferred from mutant phenotype Ref.6. Source: FlyBase exocytosis Inferred from electronic annotation. Source: UniProtKB-KW protein secretion Inferred from mutant phenotype Ref.6. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from direct assay Ref.6. Source: FlyBase Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum exit site Inferred from direct assay PubMed 19942856. Source: FlyBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VMA7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B (identifier: Q9VMA7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     923-1095: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 1430	1396	Transport and Golgi organization protein 1		PRO_0000370510
Regions
	Topological domain	35 – 796	762	Extracellular Potential
	Transmembrane	797 – 817	21	Helical; Potential
	Topological domain	818 – 1430	613	Cytoplasmic Potential
	Domain	53 – 111	59	SH3
	Coiled coil	494 – 620	127	Potential
	Coiled coil	869 – 1245	377	Potential
	Compositional bias	161 – 164	4	Poly-Thr
	Compositional bias	630 – 633	4	Poly-His
	Compositional bias	1247 – 1253	7	Poly-Gly
	Compositional bias	1277 – 1333	57	Pro-rich
	Compositional bias	1365 – 1368	4	Poly-Arg
Amino acid modifications
	Modified residue	865	1	Phosphoserine Ref.5
	Modified residue	868	1	Phosphoserine Ref.5
	Modified residue	1345	1	Phosphoserine Ref.5
	Modified residue	1348	1	Phosphoserine Ref.5
	Modified residue	1390	1	Phosphoserine Ref.5
	Modified residue	1392	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	923 – 1095	173	Missing in isoform B.		VSP_036932

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified October 1, 2002. Version 2. Checksum: FCF9988A95979349	FASTA	1,430	159,052
	10 20 30 40 50 60 MRLTNEKATM QPQLSDLALV LGLLICCLPT LTWAATLSDK RLCADPKCEQ IISMGIAKIT 70 80 90 100 110 120 YAIGGEGLIS FKINSPIRVL SKSAGSNMQL WGVDINGRRG YANKDFIMEK KILVRDKDLL 130 140 150 160 170 180 YEVPVVGPGS PVQSVETPVQ SVETTVQPVL NASESTDDLA TTTTSPLEIA VDSIVVEHDK 190 200 210 220 230 240 LQDQQVPDPT AASKAQVQVI EGTELPLEAI AATTEGSIVP ETAADPQEAT NLDSTVVDTK 250 260 270 280 290 300 EPQALNSEAI KLQEEPKAQQ PATEAEKPPP LPQAINAELE DADDFDYGDD ETDDDSQQGS 310 320 330 340 350 360 QDNESIVEIA NDNKSINESI ELKPLSVAQL KKTDKVEDSK DETKEKHAEM EVSKQEDSSL 370 380 390 400 410 420 PTETLNVTAL EEQIDQKEFP KQVLDAAVEL KSSDPLPVEE VTETVAEPPR TIVEDKINEE 430 440 450 460 470 480 IVPVSAKIQA KPATVNPTEP IVAQSDAEIK APSESVISST TPAPVVEEAP QKADPVGLPP 490 500 510 520 530 540 LFEKKNFENP NNYYKQLQEE QEKQRLVAEA EEQKRLQEEA DQQKRLQEEA ALNKRLLEEA 550 560 570 580 590 600 EQQKRLQEEA EQQKRLQEEA ELNKRLLEEA EKQKRLHEES EQLQRSSEEA EPQLSVQEAN 610 620 630 640 650 660 MQQLNDSVDS QSNEIVDNNN RQQPEQYQQH HHHTESAFNH PSTASHTTPT PDAESPYAAV 670 680 690 700 710 720 QEETTEASQT DNHREGVGYV EPVALPATAS PVSEVPIKED AAGFGLFATI VDTVNNFIGK 730 740 750 760 770 780 DPQSDPADSS DELHRILYPG RPEVPPSQRK AEDFAPADVD GYCARFQAKD EHCHRSISLD 790 800 810 820 830 840 NFVEVMADKL VDHSQLLLCV VIAAISSLFF MFAYYCFCNS SQEGALLSKL NHLERSLLAS 850 860 870 880 890 900 HKENLIIKHD LMTTRTKLAS IEDNSFGSND MVADLKKQLE SELYEKAKLQ EQVGSLERDL 910 920 930 940 950 960 DNAAEAGLEL NKMLSEVLNG QNGDEAFMST VDELQRQLND QEKIIIEINN SLAEKSRENS 970 980 990 1000 1010 1020 ELQYTFTEAT TRLNSELKTL QEDNYELEME KSKLQTRLQE IQAETESELA KALEARNYEM 1030 1040 1050 1060 1070 1080 QKLQNQIVEL TVKWEREHGD LQTSLAKIEA LEDCLKAVGK DAIHNVQELI TSAKTRGELN 1090 1100 1110 1120 1130 1140 AVHKKLVELQ SKVEQEEAHK QRLESQLQQS SQDVEQLKQD FNQSERDKLE AQTRLEVLSG 1150 1160 1170 1180 1190 1200 YFREKENDLK KELSLQETKW LQHQGENAST VETQTLMKNE IQTLKSQNDE LRAEIEAQIA 1210 1220 1230 1240 1250 1260 SHKAQMGTLE NRAHESWLAA RQSERRCEEA LAEAASLRRK LTTMASGGGG VGGDPGVMEA 1270 1280 1290 1300 1310 1320 IAANGTSVLG AELKTAPSPL PLPGSPLLNM PNPLPFLAAP FSPFMGLPPP FLPPTGAGGA 1330 1340 1350 1360 1370 1380 RPPPLGRMRS PPPSSRGDRD RERYSDYSDY DDYDDDEEDD RGMDRRRRHS GSWGRRHRGS 1390 1400 1410 1420 1430 YSHSPRTYRS LSPSDSRYNY NDTETDFSPP PSPPPVPSGR SATSRPYSEV
	Isoform B [UniParc]. Checksum: 5843F0540EEA9511	FASTA	1,257	139,137
	10 20 30 40 50 60 MRLTNEKATM QPQLSDLALV LGLLICCLPT LTWAATLSDK RLCADPKCEQ IISMGIAKIT 70 80 90 100 110 120 YAIGGEGLIS FKINSPIRVL SKSAGSNMQL WGVDINGRRG YANKDFIMEK KILVRDKDLL 130 140 150 160 170 180 YEVPVVGPGS PVQSVETPVQ SVETTVQPVL NASESTDDLA TTTTSPLEIA VDSIVVEHDK 190 200 210 220 230 240 LQDQQVPDPT AASKAQVQVI EGTELPLEAI AATTEGSIVP ETAADPQEAT NLDSTVVDTK 250 260 270 280 290 300 EPQALNSEAI KLQEEPKAQQ PATEAEKPPP LPQAINAELE DADDFDYGDD ETDDDSQQGS 310 320 330 340 350 360 QDNESIVEIA NDNKSINESI ELKPLSVAQL KKTDKVEDSK DETKEKHAEM EVSKQEDSSL 370 380 390 400 410 420 PTETLNVTAL EEQIDQKEFP KQVLDAAVEL KSSDPLPVEE VTETVAEPPR TIVEDKINEE 430 440 450 460 470 480 IVPVSAKIQA KPATVNPTEP IVAQSDAEIK APSESVISST TPAPVVEEAP QKADPVGLPP 490 500 510 520 530 540 LFEKKNFENP NNYYKQLQEE QEKQRLVAEA EEQKRLQEEA DQQKRLQEEA ALNKRLLEEA 550 560 570 580 590 600 EQQKRLQEEA EQQKRLQEEA ELNKRLLEEA EKQKRLHEES EQLQRSSEEA EPQLSVQEAN 610 620 630 640 650 660 MQQLNDSVDS QSNEIVDNNN RQQPEQYQQH HHHTESAFNH PSTASHTTPT PDAESPYAAV 670 680 690 700 710 720 QEETTEASQT DNHREGVGYV EPVALPATAS PVSEVPIKED AAGFGLFATI VDTVNNFIGK 730 740 750 760 770 780 DPQSDPADSS DELHRILYPG RPEVPPSQRK AEDFAPADVD GYCARFQAKD EHCHRSISLD 790 800 810 820 830 840 NFVEVMADKL VDHSQLLLCV VIAAISSLFF MFAYYCFCNS SQEGALLSKL NHLERSLLAS 850 860 870 880 890 900 HKENLIIKHD LMTTRTKLAS IEDNSFGSND MVADLKKQLE SELYEKAKLQ EQVGSLERDL 910 920 930 940 950 960 DNAAEAGLEL NKMLSEVLNG QNEEAHKQRL ESQLQQSSQD VEQLKQDFNQ SERDKLEAQT 970 980 990 1000 1010 1020 RLEVLSGYFR EKENDLKKEL SLQETKWLQH QGENASTVET QTLMKNEIQT LKSQNDELRA 1030 1040 1050 1060 1070 1080 EIEAQIASHK AQMGTLENRA HESWLAARQS ERRCEEALAE AASLRRKLTT MASGGGGVGG 1090 1100 1110 1120 1130 1140 DPGVMEAIAA NGTSVLGAEL KTAPSPLPLP GSPLLNMPNP LPFLAAPFSP FMGLPPPFLP 1150 1160 1170 1180 1190 1200 PTGAGGARPP PLGRMRSPPP SSRGDRDRER YSDYSDYDDY DDDEEDDRGM DRRRRHSGSW 1210 1220 1230 1240 1250 GRRHRGSYSH SPRTYRSLSP SDSRYNYNDT ETDFSPPPSP PPVPSGRSAT SRPYSEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Head.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 472-1430 (ISOFORM B). Strain: Berkeley. Tissue: Embryo.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-865; SER-868; SER-1345; SER-1348; SER-1390 AND SER-1392, MASS SPECTROMETRY. Tissue: Embryo.
[6]	"Functional genomics reveals genes involved in protein secretion and Golgi organization." Bard F., Casano L., Mallabiabarrena A., Wallace E., Saito K., Kitayama H., Guizzunti G., Hu Y., Wendler F., Dasgupta R., Perrimon N., Malhotra V. Nature 439:604-607(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014134 Genomic DNA. Translation: AAF52414.2. AE014134 Genomic DNA. Translation: AAF52413.2. BT003314 mRNA. Translation: AAO25074.1. AY075391 mRNA. Translation: AAL68225.1. Sequence problems.
RefSeq	NP_609058.2. NM_135214.4. NP_723198.2. NM_164697.3.
UniGene	Dm.330.
3D structure databases
HSSP	HSSP built from PDB template 1I1J based on UniProtKB Q16674.
ProteinModelPortal	Q9VMA7.
ModBase	Search...
Protein-protein interaction databases
STRING	7227.FBpp0078925.
Proteomic databases
PaxDb	Q9VMA7.
PRIDE	Q9VMA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0079295; FBpp0078925; FBgn0031842. FBtr0303224; FBpp0292316; FBgn0031842.
GeneID	33930.
KEGG	dme:Dmel_CG11098.
UCSC	CG11098-RA. d. melanogaster.
Organism-specific databases
CTD	33930.
FlyBase	FBgn0031842. Tango1.
Phylogenomic databases
eggNOG	NOG133684.
GeneTree	ENSGT00530000063635.
InParanoid	Q9VMA7.
OMA	QASETEH.
OrthoDB	EOG46Q581.
PhylomeDB	Q9VMA7.
Gene expression databases
Bgee	Q9VMA7.
Family and domain databases
InterPro	IPR001452. SH3_domain. [Graphical view]
SMART	SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50002. SH3. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	33930.
NextBio	785974.

Entry information

Entry name

TGO1_DROME

Accession

Primary (citable) accession number: Q9VMA7
Secondary accession number(s): Q8SY42, Q9VMA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project