Q9VLP9 (PORED_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyprenol reductase
EC=1.3.1.94

Gene names

ORF Names:

CG7840

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism By similarity.
Catalytic activity	Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.
Sequence caution	The sequence AAM52687.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dolichol metabolic process Inferred from sequence or structural similarity. Source: UniProtKB dolichol-linked oligosaccharide biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB polyprenol catabolic process Inferred from sequence or structural similarity. Source: UniProtKB protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	oxidoreductase activity, acting on the CH-CH group of donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
unc-119	Q9XYQ2	1	EBI-88569,EBI-127298

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Polyprenol reductase		PRO_0000398654
Regions
	Transmembrane	26 – 46	21	Helical; Potential
	Transmembrane	84 – 104	21	Helical; Potential
	Transmembrane	167 – 187	21	Helical; Potential
	Transmembrane	212 – 232	21	Helical; Potential
	Transmembrane	256 – 276	21	Helical; Potential
Natural variations
	Natural variant	9	1	L → P in strain: ZBMEL95 and ZBMEL157.
	Natural variant	96	1	A → V in strain: MEL11, ZBMEL145, ZBMEL131, ZBMEL229 and ZBMEL384.
	Natural variant	125	1	R → H in strain: MEL01, MEL02, MEL12, MEL13, MEL14, MEL15, MEL16, MEL17, MEL18, MEL19, MEL20, ZBMEL84, ZBMEL95, ZBMEL131, ZBMEL157, ZBMEL191, ZBMEL377 and ZBMEL398.
	Natural variant	239	1	P → R in strain: ZBMEL186.

Sequences

	Sequence		Length	Mass (Da)
	Q9VLP9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B1DF380F2631AD90	FASTA	326	38,034
	10 20 30 40 50 60 MAPPENGILE VLENLLDRYK INLLQMMFGT FIATIVFFGG LMTFVEKYLP NSIRQSFRYG 70 80 90 100 110 120 KHSFKGETDP LVAWLEVPKS WFKHFYTFAL FWSWLAFYVL VSTVREQKEA PEYVLQFLDI 130 140 150 160 170 180 MGGGRSHRKV EIDSTTACVG AFMLTLQCTR RFYETNFVQI FSKKSKINLS HYAVGYVHYF 190 200 210 220 230 240 GAVIALLSNT SGFVRGSKPM EFSLDKLTSQ QILYLGVFFL AWQQQYASNM ILVNLRKDPR 250 260 270 280 290 300 TGSVKTEKHL LPKGGLFNLL SSPHMFLEVV MYFCIADLYM PVRIWRLIFL WVASNQTINA 310 320 LLTHKWYQET FREYPKNRRA IIPFLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Widespread adaptive evolution of Drosophila genes with sex-biased expression." Proeschel M., Zhang Z., Parsch J. Genetics 174:893-900(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ZBMEL131, ZBMEL145, ZBMEL157, ZBMEL186, ZBMEL191, ZBMEL229, ZBMEL377, ZBMEL384, ZBMEL398, ZBMEL84 and ZBMEL95.
[2]	"The influence of demography and weak selection on the McDonald-Kreitman test: an empirical study in Drosophila." Parsch J., Zhang Z., Baines J.F. Mol. Biol. Evol. 26:691-698(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MEL01, MEL02, MEL11, MEL12, MEL13, MEL14, MEL15, MEL16, MEL17, MEL18, MEL19 and MEL20.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM294298 Genomic DNA. Translation: CAL26216.1. AM294299 Genomic DNA. Translation: CAL26217.1. AM294300 Genomic DNA. Translation: CAL26218.1. AM294301 Genomic DNA. Translation: CAL26219.1. AM294302 Genomic DNA. Translation: CAL26220.1. AM294303 Genomic DNA. Translation: CAL26221.1. AM294304 Genomic DNA. Translation: CAL26230.1. AM294305 Genomic DNA. Translation: CAL26235.1. AM294306 Genomic DNA. Translation: CAL26236.1. AM294307 Genomic DNA. Translation: CAL26237.1. AM294308 Genomic DNA. Translation: CAL26238.1. FM245364 Genomic DNA. Translation: CAR93290.1. FM245365 Genomic DNA. Translation: CAR93291.1. FM245366 Genomic DNA. Translation: CAR93292.1. FM245367 Genomic DNA. Translation: CAR93293.1. FM245368 Genomic DNA. Translation: CAR93294.1. FM245369 Genomic DNA. Translation: CAR93295.1. FM245370 Genomic DNA. Translation: CAR93296.1. FM245371 Genomic DNA. Translation: CAR93297.1. FM245372 Genomic DNA. Translation: CAR93298.1. FM245373 Genomic DNA. Translation: CAR93299.1. FM245374 Genomic DNA. Translation: CAR93300.1. FM245375 Genomic DNA. Translation: CAR93301.1. AE014134 Genomic DNA. Translation: AAF52635.1. AY122175 mRNA. Translation: AAM52687.1. Different initiation.
RefSeq	NP_609203.1. NM_135359.3.
UniGene	Dm.23194.
3D structure databases
ProteinModelPortal	Q9VLP9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VLP9. 1 interaction.
MINT	MINT-313018.
STRING	7227.FBpp0079218.
Proteomic databases
PaxDb	Q9VLP9.
PRIDE	Q9VLP9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0079598; FBpp0079218; FBgn0032014.
GeneID	34136.
KEGG	dme:Dmel_CG7840.
UCSC	CG7840-RA. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0032014. CG7840.
Phylogenomic databases
eggNOG	NOG330066.
GeneTree	ENSGT00500000044920.
InParanoid	Q9VLP9.
KO	K12345.
OMA	SSPHMFF.
OrthoDB	EOG41VHJM.
PhylomeDB	Q9VLP9.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Bgee	Q9VLP9.
Family and domain databases
InterPro	IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	34136.
NextBio	787042.

Entry information

Entry name

PORED_DROME

Accession

Primary (citable) accession number: Q9VLP9
Secondary accession number(s): A0ANR0 , A0ANR1, A0ANR2, A0ANR3, A0ANR5, Q8MR21

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project