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Protein

Polyprenol reductase

Gene

CG7840

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism (By similarity).By similarity

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

ReactomeiR-DME-193048. Androgen biosynthesis.
R-DME-446199. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Gene namesi
ORF Names:CG7840
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0032014. CG7840.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Transmembranei84 – 104HelicalSequence analysisAdd BLAST21
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003986541 – 326Polyprenol reductaseAdd BLAST326

Proteomic databases

PaxDbiQ9VLP9.
PRIDEiQ9VLP9.

Expressioni

Gene expression databases

BgeeiFBgn0032014.
GenevisibleiQ9VLP9. DM.

Interactioni

Protein-protein interaction databases

BioGridi60262. 1 interactor.
IntActiQ9VLP9. 1 interactor.
MINTiMINT-313018.
STRINGi7227.FBpp0079218.

Structurei

3D structure databases

ProteinModelPortaliQ9VLP9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1640. Eukaryota.
ENOG4111JQM. LUCA.
GeneTreeiENSGT00500000044920.
InParanoidiQ9VLP9.
KOiK12345.
OMAiRRFYETN.
OrthoDBiEOG091G0F09.
PhylomeDBiQ9VLP9.

Family and domain databases

InterProiView protein in InterPro
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
PfamiView protein in Pfam
PF02544. Steroid_dh. 1 hit.
PROSITEiView protein in PROSITE
PS50244. S5A_REDUCTASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VLP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPENGILE VLENLLDRYK INLLQMMFGT FIATIVFFGG LMTFVEKYLP
60 70 80 90 100
NSIRQSFRYG KHSFKGETDP LVAWLEVPKS WFKHFYTFAL FWSWLAFYVL
110 120 130 140 150
VSTVREQKEA PEYVLQFLDI MGGGRSHRKV EIDSTTACVG AFMLTLQCTR
160 170 180 190 200
RFYETNFVQI FSKKSKINLS HYAVGYVHYF GAVIALLSNT SGFVRGSKPM
210 220 230 240 250
EFSLDKLTSQ QILYLGVFFL AWQQQYASNM ILVNLRKDPR TGSVKTEKHL
260 270 280 290 300
LPKGGLFNLL SSPHMFLEVV MYFCIADLYM PVRIWRLIFL WVASNQTINA
310 320
LLTHKWYQET FREYPKNRRA IIPFLL
Length:326
Mass (Da):38,034
Last modified:May 1, 2000 - v1
Checksum:iB1DF380F2631AD90
GO

Sequence cautioni

The sequence AAM52687 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti9L → P in strain: ZBMEL95 and ZBMEL157. 1
Natural varianti96A → V in strain: MEL11, ZBMEL145, ZBMEL131, ZBMEL229 and ZBMEL384. 1
Natural varianti125R → H in strain: MEL01, MEL02, MEL12, MEL13, MEL14, MEL15, MEL16, MEL17, MEL18, MEL19, MEL20, ZBMEL84, ZBMEL95, ZBMEL131, ZBMEL157, ZBMEL191, ZBMEL377 and ZBMEL398. 1
Natural varianti239P → R in strain: ZBMEL186. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM294298 Genomic DNA. Translation: CAL26216.1.
AM294299 Genomic DNA. Translation: CAL26217.1.
AM294300 Genomic DNA. Translation: CAL26218.1.
AM294301 Genomic DNA. Translation: CAL26219.1.
AM294302 Genomic DNA. Translation: CAL26220.1.
AM294303 Genomic DNA. Translation: CAL26221.1.
AM294304 Genomic DNA. Translation: CAL26230.1.
AM294305 Genomic DNA. Translation: CAL26235.1.
AM294306 Genomic DNA. Translation: CAL26236.1.
AM294307 Genomic DNA. Translation: CAL26237.1.
AM294308 Genomic DNA. Translation: CAL26238.1.
FM245364 Genomic DNA. Translation: CAR93290.1.
FM245365 Genomic DNA. Translation: CAR93291.1.
FM245366 Genomic DNA. Translation: CAR93292.1.
FM245367 Genomic DNA. Translation: CAR93293.1.
FM245368 Genomic DNA. Translation: CAR93294.1.
FM245369 Genomic DNA. Translation: CAR93295.1.
FM245370 Genomic DNA. Translation: CAR93296.1.
FM245371 Genomic DNA. Translation: CAR93297.1.
FM245372 Genomic DNA. Translation: CAR93298.1.
FM245373 Genomic DNA. Translation: CAR93299.1.
FM245374 Genomic DNA. Translation: CAR93300.1.
FM245375 Genomic DNA. Translation: CAR93301.1.
AE014134 Genomic DNA. Translation: AAF52635.1.
AY122175 mRNA. Translation: AAM52687.1. Different initiation.
RefSeqiNP_609203.1. NM_135359.3.
UniGeneiDm.23194.

Genome annotation databases

EnsemblMetazoaiFBtr0079598; FBpp0079218; FBgn0032014.
GeneIDi34136.
KEGGidme:Dmel_CG7840.
UCSCiCG7840-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiPORED_DROME
AccessioniPrimary (citable) accession number: Q9VLP9
Secondary accession number(s): A0ANR0
, A0ANR1, A0ANR2, A0ANR3, A0ANR5, Q8MR21
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 2000
Last modified: September 27, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families