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Q9VLP9

- PORED_DROME

UniProt

Q9VLP9 - PORED_DROME

Protein

Polyprenol reductase

Gene

CG7840

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism By similarity.By similarity

    Catalytic activityi

    Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

    Pathwayi

    GO - Molecular functioni

    1. oxidoreductase activity, acting on the CH-CH group of donors Source: InterPro

    GO - Biological processi

    1. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
    2. dolichol metabolic process Source: UniProtKB
    3. polyprenol catabolic process Source: UniProtKB
    4. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_181992. Synthesis of Dolichyl-phosphate.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyprenol reductase (EC:1.3.1.94)
    Gene namesi
    ORF Names:CG7840
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0032014. CG7840.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Polyprenol reductasePRO_0000398654Add
    BLAST

    Proteomic databases

    PaxDbiQ9VLP9.
    PRIDEiQ9VLP9.

    Expressioni

    Gene expression databases

    BgeeiQ9VLP9.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    unc-119Q9XYQ21EBI-88569,EBI-127298

    Protein-protein interaction databases

    BioGridi60262. 1 interaction.
    IntActiQ9VLP9. 1 interaction.
    MINTiMINT-313018.
    STRINGi7227.FBpp0079218.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VLP9.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei26 – 4621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei84 – 10421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei167 – 18721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei212 – 23221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei256 – 27621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG330066.
    GeneTreeiENSGT00500000044920.
    InParanoidiQ9VLP9.
    KOiK12345.
    OMAiSSPHMFF.
    OrthoDBiEOG72ZCFT.
    PhylomeDBiQ9VLP9.

    Family and domain databases

    InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9VLP9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPPENGILE VLENLLDRYK INLLQMMFGT FIATIVFFGG LMTFVEKYLP    50
    NSIRQSFRYG KHSFKGETDP LVAWLEVPKS WFKHFYTFAL FWSWLAFYVL 100
    VSTVREQKEA PEYVLQFLDI MGGGRSHRKV EIDSTTACVG AFMLTLQCTR 150
    RFYETNFVQI FSKKSKINLS HYAVGYVHYF GAVIALLSNT SGFVRGSKPM 200
    EFSLDKLTSQ QILYLGVFFL AWQQQYASNM ILVNLRKDPR TGSVKTEKHL 250
    LPKGGLFNLL SSPHMFLEVV MYFCIADLYM PVRIWRLIFL WVASNQTINA 300
    LLTHKWYQET FREYPKNRRA IIPFLL 326
    Length:326
    Mass (Da):38,034
    Last modified:May 1, 2000 - v1
    Checksum:iB1DF380F2631AD90
    GO

    Sequence cautioni

    The sequence AAM52687.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti9 – 91L → P in strain: ZBMEL95 and ZBMEL157.
    Natural varianti96 – 961A → V in strain: MEL11, ZBMEL145, ZBMEL131, ZBMEL229 and ZBMEL384.
    Natural varianti125 – 1251R → H in strain: MEL01, MEL02, MEL12, MEL13, MEL14, MEL15, MEL16, MEL17, MEL18, MEL19, MEL20, ZBMEL84, ZBMEL95, ZBMEL131, ZBMEL157, ZBMEL191, ZBMEL377 and ZBMEL398.
    Natural varianti239 – 2391P → R in strain: ZBMEL186.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM294298 Genomic DNA. Translation: CAL26216.1.
    AM294299 Genomic DNA. Translation: CAL26217.1.
    AM294300 Genomic DNA. Translation: CAL26218.1.
    AM294301 Genomic DNA. Translation: CAL26219.1.
    AM294302 Genomic DNA. Translation: CAL26220.1.
    AM294303 Genomic DNA. Translation: CAL26221.1.
    AM294304 Genomic DNA. Translation: CAL26230.1.
    AM294305 Genomic DNA. Translation: CAL26235.1.
    AM294306 Genomic DNA. Translation: CAL26236.1.
    AM294307 Genomic DNA. Translation: CAL26237.1.
    AM294308 Genomic DNA. Translation: CAL26238.1.
    FM245364 Genomic DNA. Translation: CAR93290.1.
    FM245365 Genomic DNA. Translation: CAR93291.1.
    FM245366 Genomic DNA. Translation: CAR93292.1.
    FM245367 Genomic DNA. Translation: CAR93293.1.
    FM245368 Genomic DNA. Translation: CAR93294.1.
    FM245369 Genomic DNA. Translation: CAR93295.1.
    FM245370 Genomic DNA. Translation: CAR93296.1.
    FM245371 Genomic DNA. Translation: CAR93297.1.
    FM245372 Genomic DNA. Translation: CAR93298.1.
    FM245373 Genomic DNA. Translation: CAR93299.1.
    FM245374 Genomic DNA. Translation: CAR93300.1.
    FM245375 Genomic DNA. Translation: CAR93301.1.
    AE014134 Genomic DNA. Translation: AAF52635.1.
    AY122175 mRNA. Translation: AAM52687.1. Different initiation.
    RefSeqiNP_609203.1. NM_135359.3.
    UniGeneiDm.23194.

    Genome annotation databases

    EnsemblMetazoaiFBtr0079598; FBpp0079218; FBgn0032014.
    GeneIDi34136.
    KEGGidme:Dmel_CG7840.
    UCSCiCG7840-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM294298 Genomic DNA. Translation: CAL26216.1 .
    AM294299 Genomic DNA. Translation: CAL26217.1 .
    AM294300 Genomic DNA. Translation: CAL26218.1 .
    AM294301 Genomic DNA. Translation: CAL26219.1 .
    AM294302 Genomic DNA. Translation: CAL26220.1 .
    AM294303 Genomic DNA. Translation: CAL26221.1 .
    AM294304 Genomic DNA. Translation: CAL26230.1 .
    AM294305 Genomic DNA. Translation: CAL26235.1 .
    AM294306 Genomic DNA. Translation: CAL26236.1 .
    AM294307 Genomic DNA. Translation: CAL26237.1 .
    AM294308 Genomic DNA. Translation: CAL26238.1 .
    FM245364 Genomic DNA. Translation: CAR93290.1 .
    FM245365 Genomic DNA. Translation: CAR93291.1 .
    FM245366 Genomic DNA. Translation: CAR93292.1 .
    FM245367 Genomic DNA. Translation: CAR93293.1 .
    FM245368 Genomic DNA. Translation: CAR93294.1 .
    FM245369 Genomic DNA. Translation: CAR93295.1 .
    FM245370 Genomic DNA. Translation: CAR93296.1 .
    FM245371 Genomic DNA. Translation: CAR93297.1 .
    FM245372 Genomic DNA. Translation: CAR93298.1 .
    FM245373 Genomic DNA. Translation: CAR93299.1 .
    FM245374 Genomic DNA. Translation: CAR93300.1 .
    FM245375 Genomic DNA. Translation: CAR93301.1 .
    AE014134 Genomic DNA. Translation: AAF52635.1 .
    AY122175 mRNA. Translation: AAM52687.1 . Different initiation.
    RefSeqi NP_609203.1. NM_135359.3.
    UniGenei Dm.23194.

    3D structure databases

    ProteinModelPortali Q9VLP9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 60262. 1 interaction.
    IntActi Q9VLP9. 1 interaction.
    MINTi MINT-313018.
    STRINGi 7227.FBpp0079218.

    Proteomic databases

    PaxDbi Q9VLP9.
    PRIDEi Q9VLP9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0079598 ; FBpp0079218 ; FBgn0032014 .
    GeneIDi 34136.
    KEGGi dme:Dmel_CG7840.
    UCSCi CG7840-RA. d. melanogaster.

    Organism-specific databases

    FlyBasei FBgn0032014. CG7840.

    Phylogenomic databases

    eggNOGi NOG330066.
    GeneTreei ENSGT00500000044920.
    InParanoidi Q9VLP9.
    KOi K12345.
    OMAi SSPHMFF.
    OrthoDBi EOG72ZCFT.
    PhylomeDBi Q9VLP9.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_181992. Synthesis of Dolichyl-phosphate.

    Miscellaneous databases

    GenomeRNAii 34136.
    NextBioi 787042.
    PROi Q9VLP9.

    Gene expression databases

    Bgeei Q9VLP9.

    Family and domain databases

    InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Widespread adaptive evolution of Drosophila genes with sex-biased expression."
      Proeschel M., Zhang Z., Parsch J.
      Genetics 174:893-900(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ZBMEL131, ZBMEL145, ZBMEL157, ZBMEL186, ZBMEL191, ZBMEL229, ZBMEL377, ZBMEL384, ZBMEL398, ZBMEL84 and ZBMEL95.
    2. "The influence of demography and weak selection on the McDonald-Kreitman test: an empirical study in Drosophila."
      Parsch J., Zhang Z., Baines J.F.
      Mol. Biol. Evol. 26:691-698(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: MEL01, MEL02, MEL11, MEL12, MEL13, MEL14, MEL15, MEL16, MEL17, MEL18, MEL19 and MEL20.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiPORED_DROME
    AccessioniPrimary (citable) accession number: Q9VLP9
    Secondary accession number(s): A0ANR0
    , A0ANR1, A0ANR2, A0ANR3, A0ANR5, Q8MR21
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3