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Protein

Ubiquitin thioesterase otubain-like

Gene

CG4968

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Possible hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei72By similarity1
Active sitei75NucleophileBy similarity1
Binding sitei168Substrate; via carbonyl oxygenBy similarity1
Active sitei255By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DME-5689880. Ub-specific processing proteases.
R-DME-5689896. Ovarian tumor domain proteases.

Protein family/group databases

MEROPSiC65.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase otubain-like (EC:3.4.19.12By similarity)
Alternative name(s):
Ubiquitin-specific-processing protease otubain-like
Short name:
Deubiquitinating enzyme otubain-like
Gene namesi
ORF Names:CG4968
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0032214. CG4968.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002210121 – 262Ubiquitin thioesterase otubain-likeAdd BLAST262

Proteomic databases

PaxDbiQ9VL00.
PRIDEiQ9VL00.

Expressioni

Gene expression databases

BgeeiFBgn0032214.
GenevisibleiQ9VL00. DM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi60470. 2 interactors.
IntActiQ9VL00. 1 interactor.
MINTiMINT-327859.
STRINGi7227.FBpp0079577.

Structurei

3D structure databases

ProteinModelPortaliQ9VL00.
SMRiQ9VL00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 262OTUPROSITE-ProRule annotationAdd BLAST199

Sequence similaritiesi

Belongs to the peptidase C65 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3991. Eukaryota.
ENOG410ZMN7. LUCA.
GeneTreeiENSGT00390000006979.
InParanoidiQ9VL00.
KOiK09602.
OMAiEDFHETF.
OrthoDBiEOG091G0GDO.
PhylomeDBiQ9VL00.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR016615. Otubain.
IPR019400. Peptidase_C65_otubain.
[Graphical view]
PfamiPF10275. Peptidase_C65. 1 hit.
[Graphical view]
PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VL00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPFTHNDGN RDELIIQQKR DIEKEISDTT PLVSEQLPLT CLYAEYSGDE
60 70 80 90 100
IFTAKIQDLS KKYKFIRRTR PDGNCFFRAF AYSYLEYLIS NTSAYQEFKK
110 120 130 140 150
LAEESKEKLV QLGFPSFTLE DFHETFMEVI QRVSPDNAGG HSTVQDELHK
160 170 180 190 200
IFNEQGYSDY VVVYLRLITS GKLQEEADFY QNFIEGDLTI EAFRHLEVEP
210 220 230 240 250
MYKESDHIHI IALCTALGAG VRVEYLDRGE GGTVKAHDFP EGSEPRIYLI
260
YRPGHYDILY PN
Length:262
Mass (Da):30,372
Last modified:May 1, 2000 - v1
Checksum:i5F65662D161DFCD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF52905.1.
AY061382 mRNA. Translation: AAL28930.1.
RefSeqiNP_609375.1. NM_135531.3.
UniGeneiDm.385.

Genome annotation databases

EnsemblMetazoaiFBtr0079987; FBpp0079577; FBgn0032214.
GeneIDi34384.
KEGGidme:Dmel_CG4968.
UCSCiCG4968-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF52905.1.
AY061382 mRNA. Translation: AAL28930.1.
RefSeqiNP_609375.1. NM_135531.3.
UniGeneiDm.385.

3D structure databases

ProteinModelPortaliQ9VL00.
SMRiQ9VL00.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60470. 2 interactors.
IntActiQ9VL00. 1 interactor.
MINTiMINT-327859.
STRINGi7227.FBpp0079577.

Protein family/group databases

MEROPSiC65.001.

Proteomic databases

PaxDbiQ9VL00.
PRIDEiQ9VL00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079987; FBpp0079577; FBgn0032214.
GeneIDi34384.
KEGGidme:Dmel_CG4968.
UCSCiCG4968-RA. d. melanogaster.

Organism-specific databases

FlyBaseiFBgn0032214. CG4968.

Phylogenomic databases

eggNOGiKOG3991. Eukaryota.
ENOG410ZMN7. LUCA.
GeneTreeiENSGT00390000006979.
InParanoidiQ9VL00.
KOiK09602.
OMAiEDFHETF.
OrthoDBiEOG091G0GDO.
PhylomeDBiQ9VL00.

Enzyme and pathway databases

ReactomeiR-DME-5689880. Ub-specific processing proteases.
R-DME-5689896. Ovarian tumor domain proteases.

Miscellaneous databases

GenomeRNAii34384.
PROiQ9VL00.

Gene expression databases

BgeeiFBgn0032214.
GenevisibleiQ9VL00. DM.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR016615. Otubain.
IPR019400. Peptidase_C65_otubain.
[Graphical view]
PfamiPF10275. Peptidase_C65. 1 hit.
[Graphical view]
PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTUBL_DROME
AccessioniPrimary (citable) accession number: Q9VL00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.