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Q9VL00

- OTUBL_DROME

UniProt

Q9VL00 - OTUBL_DROME

Protein

Ubiquitin thioesterase otubain-like

Gene

CG4968

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Possible hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei72 – 721By similarity
    Active sitei75 – 751NucleophileBy similarity
    Active sitei255 – 2551By similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. omega peptidase activity Source: InterPro

    GO - Biological processi

    1. cellular amino acid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC65.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase otubain-like (EC:3.4.19.12)
    Alternative name(s):
    Ubiquitin-specific-processing protease otubain-like
    Short name:
    Deubiquitinating enzyme otubain-like
    Gene namesi
    ORF Names:CG4968
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0032214. CG4968.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 262262Ubiquitin thioesterase otubain-likePRO_0000221012Add
    BLAST

    Proteomic databases

    PaxDbiQ9VL00.
    PRIDEiQ9VL00.

    Expressioni

    Gene expression databases

    BgeeiQ9VL00.

    Interactioni

    Protein-protein interaction databases

    BioGridi60470. 2 interactions.
    IntActiQ9VL00. 1 interaction.
    MINTiMINT-327859.
    STRINGi7227.FBpp0079577.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VL00.
    SMRiQ9VL00. Positions 31-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini64 – 262199OTUPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C65 family.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5539.
    GeneTreeiENSGT00390000006979.
    InParanoidiQ9VL00.
    KOiK09602.
    OMAiVEPMYKE.
    OrthoDBiEOG71K64D.
    PhylomeDBiQ9VL00.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR019400. Peptidase_C65_otubain.
    IPR016615. Ubiquitin_thioesterase_Otubain.
    [Graphical view]
    PfamiPF10275. Peptidase_C65. 1 hit.
    [Graphical view]
    PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9VL00-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPFTHNDGN RDELIIQQKR DIEKEISDTT PLVSEQLPLT CLYAEYSGDE    50
    IFTAKIQDLS KKYKFIRRTR PDGNCFFRAF AYSYLEYLIS NTSAYQEFKK 100
    LAEESKEKLV QLGFPSFTLE DFHETFMEVI QRVSPDNAGG HSTVQDELHK 150
    IFNEQGYSDY VVVYLRLITS GKLQEEADFY QNFIEGDLTI EAFRHLEVEP 200
    MYKESDHIHI IALCTALGAG VRVEYLDRGE GGTVKAHDFP EGSEPRIYLI 250
    YRPGHYDILY PN 262
    Length:262
    Mass (Da):30,372
    Last modified:May 1, 2000 - v1
    Checksum:i5F65662D161DFCD2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014134 Genomic DNA. Translation: AAF52905.1.
    AY061382 mRNA. Translation: AAL28930.1.
    RefSeqiNP_609375.1. NM_135531.3.
    UniGeneiDm.385.

    Genome annotation databases

    EnsemblMetazoaiFBtr0079987; FBpp0079577; FBgn0032214.
    GeneIDi34384.
    KEGGidme:Dmel_CG4968.
    UCSCiCG4968-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014134 Genomic DNA. Translation: AAF52905.1 .
    AY061382 mRNA. Translation: AAL28930.1 .
    RefSeqi NP_609375.1. NM_135531.3.
    UniGenei Dm.385.

    3D structure databases

    ProteinModelPortali Q9VL00.
    SMRi Q9VL00. Positions 31-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 60470. 2 interactions.
    IntActi Q9VL00. 1 interaction.
    MINTi MINT-327859.
    STRINGi 7227.FBpp0079577.

    Protein family/group databases

    MEROPSi C65.001.

    Proteomic databases

    PaxDbi Q9VL00.
    PRIDEi Q9VL00.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0079987 ; FBpp0079577 ; FBgn0032214 .
    GeneIDi 34384.
    KEGGi dme:Dmel_CG4968.
    UCSCi CG4968-RA. d. melanogaster.

    Organism-specific databases

    FlyBasei FBgn0032214. CG4968.

    Phylogenomic databases

    eggNOGi COG5539.
    GeneTreei ENSGT00390000006979.
    InParanoidi Q9VL00.
    KOi K09602.
    OMAi VEPMYKE.
    OrthoDBi EOG71K64D.
    PhylomeDBi Q9VL00.

    Miscellaneous databases

    GenomeRNAii 34384.
    NextBioi 788236.
    PROi Q9VL00.

    Gene expression databases

    Bgeei Q9VL00.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR019400. Peptidase_C65_otubain.
    IPR016615. Ubiquitin_thioesterase_Otubain.
    [Graphical view ]
    Pfami PF10275. Peptidase_C65. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiOTUBL_DROME
    AccessioniPrimary (citable) accession number: Q9VL00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3