Reviewed, UniProtKB/Swiss-Prot Q9VKY8 (SPS2_DROME)

Names and origin

Protein names

Recommended name:
    Selenide, water dikinase 2
    EC=2.7.9.3
Alternative name(s):
    Selenophosphate synthetase 2
    Selenium donor protein 2
      Short name=Dsps2

Gene names

Name:	Sps2
ORF Names:	CG5025

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Synthesizes selenophosphate from selenide and ATP.
Catalytic activity	ATP + selenide + H2O = AMP + selenophosphate + phosphate.
Tissue specificity	First expressed in the midgut anlagen with subsequent expression in a variety of tissues including the gut and nervous system. Ref.1
Developmental stage	Expressed both maternally and zygotically. Ref.1
Sequence similarities	Belongs to the selenophosphate synthetase 1 family. Class I subfamily.
Sequence caution	The sequence AAR99143.1 differs from that shown. Reason: Erroneous termination at position 24. Translated as Sec.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Selenocysteine
Ligand	ATP-binding Nucleotide-binding Selenium
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Ref.1 Traceable author statement. Source: UniProtKB
Molecular function	ATP binding Ref.1 Traceable author statement. Source: UniProtKB selenide, water dikinase activity Ref.1 Traceable author statement. Source: UniProtKB selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q9VKY8-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q9VKY8-2) The sequence of this isoform differs from the canonical sequence as follows:      279-305: VLFQINKLPIIKNVLKFSTLVGQSTKF → HLGGPKHKVSIWQIGGNLWRPFNMPSC      306-370: Missing.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
	Sequence conflict	279	1	H → P in AAF80118. Ref.2

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Selenide, water dikinase 2		PRO_0000127653
Regions
	Nucleotide binding	261 – 267	7	ATP Potential
Sites
	Active site	24	1	Potential
	Site	27	1	Important for catalytic activity By similarity
Amino acid modifications
	Non-standard residue	24	1	Selenocysteine
Natural variations
	Alternative sequence	279 – 305	27	VLFQI…QSTKF → HLGGPKHKVSIWQIGGNLWR PFNMPSC in isoform B.		VSP_050768
	Alternative sequence	306 – 370	65	Missing in isoform B.		VSP_050769
Experimental info
	Sequence conflict	189	1	L → F Ref.2
	Sequence conflict	189	1	L → F Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified February 26, 2008. Version 3. Checksum: 95128BCD133731B3	FASTA	370	41,131
	10 20 30 40 50 60 MFQPEKHGLE PDFQLTKFTT HTGUSCKIPQ KVLEKYLRGT EIENKNNDGY LIGSGMDCAV 70 80 90 100 110 120 IPLKRHKDYL LIQTVDFFYP MVNDPELLGR IALANVLSDV YAVGVTQFDT VEMIVSTSTS 130 140 150 160 170 180 FSEKERDVVI GLVMKGFQNS LKANGYRNTP LIIRQLKINP WCIIGGIATS VCRSEEIILP 190 200 210 220 230 240 SNAQPGDVLV LTKPLGGQMA MDAHLWQLNQ TEKYKKLLSE CSDADIKETF EIAVKSMTYL 250 260 270 280 290 300 NKNAALLMHK YQAHCATDIT GFGLLGHANN LAQFQKEKVL FQINKLPIIK NVLKFSTLVG 310 320 330 340 350 360 QSTKFRSGRS VETSGGLLIC LPADAADKFC RDFEEATNGE QKSFQIGHVT AANESDAVLC 370 EDVEFIEVSL
	Isoform B. Checksum: 59C8805F98948F5A	FASTA	305	34,155
	10 20 30 40 50 60 MFQPEKHGLE PDFQLTKFTT HTGUSCKIPQ KVLEKYLRGT EIENKNNDGY LIGSGMDCAV 70 80 90 100 110 120 IPLKRHKDYL LIQTVDFFYP MVNDPELLGR IALANVLSDV YAVGVTQFDT VEMIVSTSTS 130 140 150 160 170 180 FSEKERDVVI GLVMKGFQNS LKANGYRNTP LIIRQLKINP WCIIGGIATS VCRSEEIILP 190 200 210 220 230 240 SNAQPGDVLV LTKPLGGQMA MDAHLWQLNQ TEKYKKLLSE CSDADIKETF EIAVKSMTYL 250 260 270 280 290 300 NKNAALLMHK YQAHCATDIT GFGLLGHANN LAQFQKEKHL GGPKHKVSIW QIGGNLWRPF NMPSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The class 2 selenophosphate synthetase gene of Drosophila contains a functional mammalian-type SECIS." Hirosawa-Takamori M., Jackle H., Vorbruggen G. EMBO Rep. 1:441-446(2000) [PubMed: 11258485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A), PROBABLE SELENOCYSTEINE AT SEC-24, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Embryo.
[2]	"Structural analysis of new local features in SECIS RNA hairpins." Fagegaltier D., Lescure A., Walczak R., Carbon P., Krol A. Nucleic Acids Res. 28:2679-2689(2000) [PubMed: 10908323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278068 mRNA. Translation: CAB93526.2. AF279253 mRNA. Translation: AAF80118.1. AE014134 Genomic DNA. Translation: AAF52918.2. AE014134 Genomic DNA. Translation: AAN10746.2. BT011485 mRNA. Translation: AAR99143.1. Sequence problems.
RefSeq	NP_477477.4. NP_477478.4.
UniGene	Dm.3457
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VKY8. 4 interactions.
STRING	Q9VKY8.
Genome annotation databases
Ensembl	FBtr0079997; FBpp0079587; FBgn0032224; Drosophila melanogaster. [Genome view] FBtr0079998; FBpp0079588; FBgn0032224; Drosophila melanogaster. [Genome view]
GeneID	34397.
KEGG	dme:Dmel_CG5025.
Organism-specific databases
CTD	34397.
FlyBase	FBgn0032224. Sps2.
Phylogenomic databases
InParanoid	Q9VKY8.
OMA	GISANIW.
PhylomeDB	Q9VKY8.
Enzyme and pathway databases
BRENDA	2.7.9.3. 48.
Gene expression databases
Bgee	Q9VKY8.
GermOnline	CG5025. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR016188. PurM_N-like. IPR004536. SelD. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
PIRSF	PIRSF036407. Selenphspht_syn. 1 hit.
TIGRFAMs	TIGR00476. selD. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	788302.

Entry information

Entry name

SPS2_DROME

Accession

Primary (citable) accession number: Q9VKY8
Secondary accession number(s): Q8IPC0, Q9NAX3, Q9NFK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project