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Protein

Cullin-associated NEDD8-dissociated protein 1

Gene

Cand1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes (Probable).1 Publication

GO - Biological processi

  • negative regulation of protein neddylation Source: FlyBase
  • protein ubiquitination Source: GO_Central
  • regulation of protein stability Source: FlyBase
  • SCF complex assembly Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-associated NEDD8-dissociated protein 1
Alternative name(s):
Cullin-associated and neddylation-dissociated protein 1
Gene namesi
Name:Cand1
ORF Names:CG5366
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0027568. Cand1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Lethality in the pupal stage. Accumulation of neddylated Cul3 and stabilization of the Cul3 adapter protein HIB. Enhances protein degradation of Cubitus interruptus (Ci), suggesting that Cul3-RING ligase activity is enhanced in the absence of Cand1.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12481248Cullin-associated NEDD8-dissociated protein 1PRO_0000422241Add
BLAST

Proteomic databases

PaxDbiQ9VKY2.
PRIDEiQ9VKY2.

Expressioni

Gene expression databases

ExpressionAtlasiQ9VKY2. differential.
GenevisibleiQ9VKY2. DM.

Interactioni

Protein-protein interaction databases

BioGridi60488. 16 interactions.
IntActiQ9VKY2. 2 interactions.
STRINGi7227.FBpp0304507.

Structurei

3D structure databases

ProteinModelPortaliQ9VKY2.
SMRiQ9VKY2. Positions 87-117, 142-204, 429-458, 486-544.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati44 – 8239HEAT 1Add
BLAST
Repeati127 – 16539HEAT 2Add
BLAST
Repeati168 – 20639HEAT 3Add
BLAST
Repeati365 – 40339HEAT 4Add
BLAST
Repeati425 – 46339HEAT 5Add
BLAST
Repeati510 – 54839HEAT 6Add
BLAST
Repeati604 – 64239HEAT 7Add
BLAST
Repeati644 – 68239HEAT 8Add
BLAST
Repeati861 – 90040HEAT 9Add
BLAST
Repeati976 – 101439HEAT 10Add
BLAST
Repeati1054 – 109340HEAT 11Add
BLAST

Sequence similaritiesi

Belongs to the CAND family.Curated
Contains 11 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1824. Eukaryota.
ENOG410XPK4. LUCA.
GeneTreeiENSGT00390000017740.
InParanoidiQ9VKY2.
KOiK17263.
OMAiTRPAQSW.
OrthoDBiEOG77HDCZ.
PhylomeDBiQ9VKY2.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013932. TATA-bd_TIP120.
[Graphical view]
PfamiPF08623. TIP120. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VKY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASHQYHQIA NLLEKMTSTD KDFRFMATND LMTELQKDSI ILDDESEKKV
60 70 80 90 100
VRMVLKLLED KNGEVQNLAV KCLGPLVNKV KEIQVETIVD SLCANMMSNT
110 120 130 140 150
EQLRDISSIG LKTVIAELPQ SSNSLAPNVC QRITGKLSTA IEKEDVSVKL
160 170 180 190 200
ESLDILADLL SRFGEFLVPF HSTILKALMP QLASSRQAVR KRTIVALSFL
210 220 230 240 250
LIQANSNAYN GVIDHLLDGL ENPPNPAAIR TYIQCLASIC RQAGHRLCNH
260 270 280 290 300
IDRSMLLLSQ YSQRDDDELR EFCLQACEAF VMRCPDAINP HIPMILELCL
310 320 330 340 350
NYITYDPNYN YETDDGDTGN AMDTEDDEYV DSEEYSDDDD MSWKVRRAAA
360 370 380 390 400
KCLEVLISTR QELVEDFYRS LSPALIARFK EREENVKSDI FHAYVALLKN
410 420 430 440 450
TRLTDDVAND HDSMDQVSGP TSLLIEQLPL IVKAIQPLMR EKSMKTRQDC
460 470 480 490 500
FLLLRELLNS LPGALGPYLD SIVPGISYSL NDKSSTSNMK IESLGFLYSL
510 520 530 540 550
LQGHPPHVFH PHIPLLVPLV VTSVFDPFYK IATEALLVLQ QLVKVIRPLE
560 570 580 590 600
PNAAKSDFDA PSFVGQVYSC TLQKLKVTDV DQEVKERAIA CMGQIIANMG
610 620 630 640 650
DMLQNELAVC LPIFMERLKN EVTRLSSVKA LTLIAASSLR IDLTPILHDV
660 670 680 690 700
LPALGTFLRK NHRALKLHSL DLINKIVINY SSNFEANLLQ TAIVEIPPLI
710 720 730 740 750
SDSDLHVAQY SLTLLSTVAR RQPQALVGIH EQFLRSVLIL VRSPLLQGSA
760 770 780 790 800
LNCTLELFQA LVQTQLSGLD YHSLVSKLMA PVLGGNGDVK SRATAGAPSE
810 820 830 840 850
VVQLHKQAYH SSAKCIAALT QQCPQVATPL ATKLITDLQK RNDTEIIFCL
860 870 880 890 900
LTIGEIGRHF DLSSIQVLPQ TIIECFGATS EDVKAAASHA LGAVSVGSLQ
910 920 930 940 950
TYLPLILHEI EVQPKRQYLL LHSLKEVISS LSVSPSGLAQ LLPSVPSIWD
960 970 980 990 1000
QLFKHCECSE EGSRNVVAEC LGKLVLVNPD ELLPQLQQAL RSESATMRTV
1010 1020 1030 1040 1050
VVSSVKFTIS DQPQPIDVLL KQNIGEFLFA LRDPEPQVRR VALVAFNSAV
1060 1070 1080 1090 1100
HNKPSLVRDL LPTLLPWLYS ETKVKSELIR EVEMGPFKHT VDDGLDIRKA
1110 1120 1130 1140 1150
AFECMYTLLE QGLDRVDVMQ FLDHVQAGLC DHYDIKMLTY LMTARLAILC
1160 1170 1180 1190 1200
PDKVLLRLDQ FIQQLRDTCT HKVKANSVKQ EYEKQDELKR SALRAVSALS
1210 1220 1230 1240
QIPKANKNQQ LVDFLKSIKE TPELNKIFEY IQKDSITGSS DIIVMDQS
Length:1,248
Mass (Da):139,355
Last modified:May 1, 2000 - v1
Checksum:i237877E5B6F28A93
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti292 – 2921I → T in AAY84910 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF52924.1.
BT023510 mRNA. Translation: AAY84910.1.
BT133409 mRNA. Translation: AFH55508.1.
RefSeqiNP_001260333.1. NM_001273404.1.
NP_609389.1. NM_135545.3.
UniGeneiDm.3151.

Genome annotation databases

EnsemblMetazoaiFBtr0080054; FBpp0079643; FBgn0027568.
FBtr0332198; FBpp0304507; FBgn0027568.
GeneIDi34403.
KEGGidme:Dmel_CG5366.
UCSCiCG5366-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF52924.1.
BT023510 mRNA. Translation: AAY84910.1.
BT133409 mRNA. Translation: AFH55508.1.
RefSeqiNP_001260333.1. NM_001273404.1.
NP_609389.1. NM_135545.3.
UniGeneiDm.3151.

3D structure databases

ProteinModelPortaliQ9VKY2.
SMRiQ9VKY2. Positions 87-117, 142-204, 429-458, 486-544.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60488. 16 interactions.
IntActiQ9VKY2. 2 interactions.
STRINGi7227.FBpp0304507.

Proteomic databases

PaxDbiQ9VKY2.
PRIDEiQ9VKY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080054; FBpp0079643; FBgn0027568.
FBtr0332198; FBpp0304507; FBgn0027568.
GeneIDi34403.
KEGGidme:Dmel_CG5366.
UCSCiCG5366-RA. d. melanogaster.

Organism-specific databases

CTDi55832.
FlyBaseiFBgn0027568. Cand1.

Phylogenomic databases

eggNOGiKOG1824. Eukaryota.
ENOG410XPK4. LUCA.
GeneTreeiENSGT00390000017740.
InParanoidiQ9VKY2.
KOiK17263.
OMAiTRPAQSW.
OrthoDBiEOG77HDCZ.
PhylomeDBiQ9VKY2.

Miscellaneous databases

GenomeRNAii34403.
PROiQ9VKY2.

Gene expression databases

ExpressionAtlasiQ9VKY2. differential.
GenevisibleiQ9VKY2. DM.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013932. TATA-bd_TIP120.
[Graphical view]
PfamiPF08623. TIP120. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.
    Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
  4. "Drosophila Cand1 regulates Cullin3-dependent E3 ligases by affecting the neddylation of Cullin3 and by controlling the stability of Cullin3 and adaptor protein."
    Kim S.H., Kim H.J., Kim S., Yim J.
    Dev. Biol. 346:247-257(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCAND1_DROME
AccessioniPrimary (citable) accession number: Q9VKY2
Secondary accession number(s): Q4QQA6, Q9Y127
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.