Q9VKK1 (EDC4_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enhancer of mRNA-decapping protein 4 homolog

Gene names

Name:	Ge-1
ORF Names:	CG6181

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In the process of mRNA degradation, seems to play a role in mRNA decapping. Required for silencing a subset of endogenous miRNA targets. Ref.5
Subunit structure	Homodimer Probable. Interacts with Dcp1 and Dcp2. Ref.8
Subcellular location	Cytoplasm › P-body Ref.5 Ref.8.
Sequence similarities	Belongs to the WD repeat EDC4 family. Contains 2 WD repeats.
Sequence caution	The sequence AAM29374.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil Repeat WD repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic mRNA processing body assembly Inferred from mutant phenotype. Source: FlyBase mRNA catabolic process Inferred from mutant phenotype Ref.5. Source: UniProtKB
Cellular component	cytoplasmic mRNA processing body Inferred from direct assay Ref.8. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.8. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1354

1354

Enhancer of mRNA-decapping protein 4 homolog

PRO_0000372649

Regions

Repeat

309 – 348

40

WD 1

Repeat

363 – 406

44

WD 2

Coiled coil

765 – 803

39

Potential

Coiled coil

893 – 936

44

Potential

Coiled coil

969 – 1036

68

Potential

Coiled coil

1159 – 1188

30

Potential

Compositional bias

263 – 266

4

Poly-Asp

Compositional bias

589 – 662

74

Ala-rich

Compositional bias

838 – 880

43

Asn-rich

Amino acid modifications

Modified residue

32

1

Phosphoserine Ref.7

Modified residue

561

1

Phosphoserine Ref.7

Modified residue

563

1

Phosphothreonine Ref.7

Modified residue

576

1

Phosphoserine Ref.6 Ref.7

Modified residue

581

1

Phosphothreonine Ref.6

Modified residue

759

1

Phosphoserine Ref.7

Modified residue

762

1

Phosphoserine Ref.7

Modified residue

763

1

Phosphoserine Ref.7

Modified residue

1207

1

Phosphoserine Ref.7

Modified residue

1211

1

Phosphothreonine Ref.7

Modified residue

1317

1

Phosphothreonine Ref.7

Modified residue

1320

1

Phosphotyrosine Ref.7

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1354

Helix Strand Turn

Details...

Helix

1221 – 1227

7

Helix

1231 – 1240

10

Helix

1247 – 1253

7

Helix

1256 – 1259

4

Helix

1269 – 1281

13

Helix

1288 – 1300

13

Helix

1306 – 1329

24

Helix

1336 – 1349

14

Sequences

	Sequence		Length	Mass (Da)
	Q9VKK1 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: CA9E6E78D338CD2C	FASTA	1,354	149,251
	10 20 30 40 50 60 MLIALFALAH LPIFRKTPLS ASSSPPPSVH RSPRCGKAST AYHPPPADAC ISITASAIDA 70 80 90 100 110 120 ASGMSNSAEQ DKPIAANGNA AEPQEIEVIH FQAQEKQCCR VIRSNKTKVL TSGGVHTRGS 130 140 150 160 170 180 SKVKLKNIVD YKWERKYYYP GHLVAVHRDG KHLAYAINVN NKATGMEGMV RVCNIATSMR 190 200 210 220 230 240 ALIKGMSGEV LDLQFAHTDC ERILAVIDVS SLFVYKVDQI EGNLLCNLVL KVEDPIANYV 250 260 270 280 290 300 PEYDMVSWCP YVCSSSATVP INDDDDENQL LIWSRSSQFQ CFQVKMIVSE HGRGKIQPAA 310 320 330 340 350 360 LESGYLKIEE DSLITCAALS PDGTTVAAAC ADGLVRFYQI YLFDVRNHRC LHEWKPHDGK 370 380 390 400 410 420 KVCSLFFLDN INKPVEESYW QHVITTSDAN TEIKLWNCSL WKCLQTINVV ASPSSLQPRN 430 440 450 460 470 480 FIAGIDRSAN YLVLSCLDSL AVYVMQIGST GGADSENRSS DSEGEGCDTS KRIQNVAEFK 490 500 510 520 530 540 LSSGILSFSI VNASMRRVKN SIESYYPIEE PDDFDDDSNS TSALVLHMFV VQAKSLQECQ 550 560 570 580 590 600 IIYQPCVAEK TERSSLNSKR SQTPEDNLLI KEEPESPNSG TVGAVQLDAL FAKSAKRAST 610 620 630 640 650 660 GSSSAMVAVA AAAAAAPSAI LQDATKEAAK SESPQLSSAY TQQVNLMTPD AFSASGTAAA 670 680 690 700 710 720 AAVFVSTSTT TSIGTDSSTT TSGQDRSIDS AVLQTIRMLA TVTSKTSENP NAEVLLNLMN 730 740 750 760 770 780 NTLIEDREQQ KLKEKLDARK KFIAIDRNPE RNVAENLASG SSSPSREVQE IMATQDDADA 790 800 810 820 830 840 YEAELENLDD DDDDEEEELA NSSPLPEAVD GTWPIVKLSS HSAELQNAAQ IMSQAVQNTN 850 860 870 880 890 900 NGNVPPTLGG GHNNNTSVGS NSNNNTATTL STSNTSSSNN AGGTCVDSSG TGELNAKMEL 910 920 930 940 950 960 LIDLVKAQSK QINKLENEVN KLQKQQEAAA ALHSKQDTSL EPKNLSQLAY KIEMQLSKLM 970 980 990 1000 1010 1020 EQYLKRYENE HKKKLTEFLA ARESQNRELR DSVLQVLNQY VMNHFTDIIG NVLNMELQRQ 1030 1040 1050 1060 1070 1080 LLPRVNANMD QLQAQMQVEI VQKLSVFDKT VKENIAQVCK SKQFLDTFGK SVLIGVQTSL 1090 1100 1110 1120 1130 1140 QTAFIESMSS TLIPAYEKSS QNMFKQLHDA FSVGIKDFMV QFNTYLQHMP QPQAGSGNTE 1150 1160 1170 1180 1190 1200 EINNKLSMLK QLVESSLHKH RTELTDAMLE TQREVKSLEI LLARQVQETI RAELRKHMEA 1210 1220 1230 1240 1250 1260 QNVAMRSQAA TPAPPYDLRD SIKQLLMAGQ INKAFHQALL ANDLGLVEFT LRHTDSNQAF 1270 1280 1290 1300 1310 1320 APEGCRLEQK VLLSLIQQIS ADMTNHNELK QRYLNEALLA INMADPITRE HAPKVLTELY 1330 1340 1350 RNCQQFIKNS PKNSQFSNVR LLMKAIITYR DQLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 377-1354. Strain: Berkeley. Tissue: Embryo.
[5]	"Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing." Eulalio A., Rehwinkel J., Stricker M., Huntzinger E., Yang S.-F., Doerks T., Dorner S., Bork P., Boutros M., Izaurralde E. Genes Dev. 21:2558-2570(2007) [PubMed: 17901217] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-576 AND THR-581, MASS SPECTROMETRY.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-561; THR-563; SER-576; SER-759; SER-762; SER-763; SER-1207; THR-1211; THR-1317 AND TYR-1320, MASS SPECTROMETRY. Tissue: Embryo.
[8]	"The C-terminal region of Ge-1 presents conserved structural features required for P-body localization." Jinek M., Eulalio A., Lingel A., Helms S., Conti E., Izaurralde E. RNA 14:1991-1998(2008) [PubMed: 18755833] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1220-1354, SUBUNIT, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE014134 Genomic DNA. Translation: AAF53066.2.
AE014134 Genomic DNA. Translation: AAF53067.2.
BT004479 mRNA. Translation: AAO42643.1.
AY113369 mRNA. Translation: AAM29374.1. Different initiation.

RefSeq

NP_609486.2. NM_135642.2.
NP_723654.1. NM_164957.1.

UniGene

Dm.12439.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VXG	X-ray	1.90	A/B	1220-1354	[»]

ProteinModelPortal

Q9VKK1.

SMR

Q9VKK1. Positions 168-217, 310-408, 1220-1350.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9VKK1. 1 interaction.

MINT

MINT-965853.

STRING

Q9VKK1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0080231; FBpp0079818; FBgn0032340.
FBtr0080232; FBpp0079819; FBgn0032340.

GeneID

34541.

KEGG

dme:Dmel_CG6181.

UCSC

CG6181-RA. d. melanogaster.

Organism-specific databases

CTD

34541.

FlyBase

FBgn0032340. Ge-1.

Phylogenomic databases

eggNOG

inNOG08310.

GeneTree

EMGT00050000014092.

InParanoid

Q9VKK1.

OMA

ESPRNGL.

OrthoDB

EOG4N02VW.

PhylomeDB

Q9VKK1.

Gene expression databases

ArrayExpress

Q9VKK1.

Bgee

Q9VKK1.

Family and domain databases

InterPro

IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.

KO

K12616.

SMART

SM00320. WD40. 2 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. False negative.
PS50294. WD_REPEATS_REGION. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

788972.

Entry information

Entry name

EDC4_DROME

Accession

Primary (citable) accession number: Q9VKK1
Secondary accession number(s): Q8MZ42

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project