Reviewed, UniProtKB/Swiss-Prot Q9VKJ9 (C2D1_DROME)

Names and origin

Protein names

Recommended name:
    Coiled-coil and C2 domain-containing protein 1-like
Alternative name(s):
    Lethal giant disks protein

Gene names

Name:	l(2)gd1
Synonyms:	lgd
ORF Names:	CG4713

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	816 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Negative regulator of the Notch signaling pathway, acting to restrict the activity of Notch to the dorsoventral (D/V) boundary of the wing imaginal disk. Also causes negative regulation of Notch during vein, eye, and bristle development. Acts by targeting Notch for endosomal degradation or recycling. Ref.4 Ref.5
Subcellular location	Cytoplasm. Ref.5
Tissue specificity	Expressed in wing and eye imaginal disks. Ref.4 Ref.5
Disruption phenotype	Flies exhibit problems in vein, eye, and bristle development. Ref.4
Sequence similarities	Belongs to the CC2D1 family. Contains 1 C2 domain.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Cytoplasm
Domain	Coiled coil
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 816	816	Coiled-coil and C2 domain-containing protein 1-like		PRO_0000288430
Regions
	Domain	651 – 760	110	C2
	Coiled coil	354 – 385	32	Potential
	Compositional bias	87 – 92	6	Poly-Asp
	Compositional bias	111 – 117	7	Poly-Glu
	Compositional bias	197 – 258	62	Pro-rich
	Compositional bias	315 – 318	4	Poly-Pro
	Compositional bias	410 – 478	69	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9VKJ9-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: CD757E267DA491E5	FASTA	816	89,038
	10 20 30 40 50 60 MFSRKKPEPA KRRQHDLSQF GLTEIPDDFD PSAGYGEDDG GDSDLEAELA AITGGEGAKP 70 80 90 100 110 120 KPKPKAKLLP ASDLDKMIAD SLRDVSDDDD DDNLESDPDL LGELSGIGGL EEAEEEEPVA 130 140 150 160 170 180 QPPAASEEPV QTFLPTTTVD TLSIIKQRLE MYKQAEANAK TAGDSGKARR FGRGLKTLKD 190 200 210 220 230 240 LHRQAAAGKS INVDDIPPEV SVKPIGGQAP PVPAEESPAP STPASPPPVP SRAAPDPPTP 250 260 270 280 290 300 GTPVEPTTSV APTSPPNPLV TQMRSRQTDY KAAALQSKRS GDISTALQFL KVVKQFDVVI 310 320 330 340 350 360 KMCEDGQEVD LSDMPPPPAE FLEFLKKMQE EAAAEAVAEP TAAPEPTPVA PAPVLAAATN 370 380 390 400 410 420 MLEALQQRLE KYQSVEAAAK AENNSGKARR FGRIVKQYED AIKLYKAGKP VPYDELPVPP 430 440 450 460 470 480 GFGPLPTADA APVAPTPSLP TSPTSPPPTA STSAGGTPSS SSATTPTAPR KAPSPPKPKE 490 500 510 520 530 540 LTTRTSGNQQ KNNIAEQQMK LLLERQKEFK LAAIEAKKAG EIDQAKEYLK IFKGFDSLLN 550 560 570 580 590 600 AASSGLPVDL STLPVPPSQR DNLEASFAIV SAEECDPTDD ICEIGVRMEE QLAKQLMMCK 610 620 630 640 650 660 NTRDHHKAMG DVAGMNRFEN LALTVQKDLD LVRYSKRKNE PLPKFHYEKR SFNIVHCNTD 670 680 690 700 710 720 LTDSELEIVV VRGISYNVAN PKDVDTYVRV EFPLLNDESF KTKTNVIRDT SSPDYDERFK 730 740 750 760 770 780 VDIQRTNRQF QRIFKRHGVK FEIYSRGGFL RSDTLIGTVN VKLQPLETKC EIHDTYDLMD 790 800 810 GRKQVGGKLE VKIRVRNPIL TKQMEHITEK WLVLDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	"The tumour suppressor gene l(2)giant discs is required to restrict the activity of Notch to the dorsoventral boundary during Drosophila wing development." Klein T. Dev. Biol. 255:313-333(2003) [PubMed: 12648493] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[5]	"Lethal giant discs, a novel C2-domain protein, restricts notch activation during endocytosis." Childress J.L., Acar M., Tao C., Halder G. Curr. Biol. 16:2228-2233(2006) [PubMed: 17088062] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014134 Genomic DNA. Translation: AAF53069.1. AY058523 mRNA. Translation: AAL13752.1.
RefSeq	NP_609488.1.
UniGene	Dm.4175
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9VKJ9.
Genome annotation databases
Ensembl	FBgn0032342. Drosophila melanogaster. [Contig view]
GeneID	34543.
KEGG	dme:Dmel_CG4713.
NMPDR	fig|7227.3.peg.1967.
Organism-specific databases
FlyBase	FBgn0250747. l(2)gd1.
Phylogenomic databases
HOGENOM	Q9VKJ9.
OMA	Q9VKJ9. LPMAHIE.
Gene expression databases
ArrayExpress	Q9VKJ9.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR006608. DM14. [Graphical view]
Pfam	PF00168. C2. 1 hit. [Graphical view]
SMART	SM00239. C2. 1 hit. SM00685. DM14. 4 hits. [Graphical view]
PROSITE	PS50004. C2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	788984.

Entry information

Entry name

C2D1_DROME

Accession

Primary (citable) accession number: Q9VKJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project