Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase Tor

Gene

Tor

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes cell and tissue growth, maintains tissue homeostatis and controls responses to environmental stress and aging (PubMed:11069885, PubMed:11069888, PubMed:19211682, PubMed:19225150). Regulates growth during animal development by coupling growth factor signaling to nutrient availability (PubMed:11069888). Central regulators of autophagy (PubMed:18604198, PubMed:19225150). May be involved in atg1 phosphorylation (PubMed:19225150). May also be involved, directly or indirectly, in the control of neuronal function (PubMed:15454083). Phosphorylates S6K/p70S6K, in vitro (PubMed:11069888). May regulate the activity of S6K (PubMed:11069885). Overexpression inhibits growth and reduces cell size (PubMed:14505573). Affects the timing of neuronal cell differentiation (PubMed:15454083). Hyperactivation of the signaling leads to accelerated differentiation, whereas inhibition of the signaling retards differentiation (PubMed:15454083). Thus, in addition to controlling growth of the cell in which it resides, it can also influence growth of distant cells and organs during development via a humoral mechanism (PubMed:14505573). As part of the TORC1 complex regulates energy homeostasis and promotes certain aspects of larval growth by negatively regulating REPTOR (PubMed:25920570). REPTOR functions downstream of TORC1 to regulate the expression of stress response genes in response to TORC1 inhibition resulting from nutrient deprivation (PubMed:25920570). When TORC1 activity is high it phosphorylates REPTOR which inhibits its recruitment into the nucleus and antagonizes their function (PubMed:25920570). This function is essential under normal feeding conditions to promote TORC1-dependent growth during larval development and, in adults and larvae to prevent the REPTOR-dependent expression of nutrient stress response genes (PubMed:25920570). In short, during development, it primarily controls growth, whereas in the adult, where there is relatively little growth, it controls aging and other aspects of nutrient-related physiology (PubMed:11069885, PubMed:11069888, PubMed:19211682, PubMed:19225150). Rag GTPases act as activators of TORC1 in response to amino acid signals (PubMed:18604198).8 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: GO_Central
  • protein-containing complex binding Source: InterPro
  • protein kinase activity Source: FlyBase
  • protein self-association Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  • apical protein localization Source: FlyBase
  • autophagy Source: FlyBase
  • axon guidance Source: FlyBase
  • cell cycle Source: UniProtKB-KW
  • dendrite morphogenesis Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • DNA repair Source: GO_Central
  • endocytic recycling Source: FlyBase
  • germarium-derived cystoblast division Source: FlyBase
  • gonad development Source: FlyBase
  • larval midgut cell programmed cell death Source: FlyBase
  • multicellular organism growth Source: FlyBase
  • myoblast fusion Source: FlyBase
  • negative regulation of macroautophagy Source: FlyBase
  • phosphorylation Source: FlyBase
  • phototaxis Source: FlyBase
  • positive regulation of cell cycle G1/S phase transition Source: FlyBase
  • positive regulation of cell growth Source: FlyBase
  • positive regulation of cell size Source: FlyBase
  • positive regulation of multicellular organism growth Source: FlyBase
  • positive regulation of protein phosphorylation Source: FlyBase
  • positive regulation of ribosome biogenesis Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of cell growth Source: FlyBase
  • regulation of reactive oxygen species metabolic process Source: FlyBase
  • regulation of response to drug Source: FlyBase
  • regulation of terminal button organization Source: FlyBase
  • response to nutrient Source: FlyBase
  • somatic muscle development Source: FlyBase
  • synaptic growth at neuromuscular junction Source: FlyBase
  • terminal branching, open tracheal system Source: FlyBase
  • TORC1 signaling Source: UniProtKB

Keywordsi

Molecular functionKinase, Transferase
Biological processCell cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1257604 PIP3 activates AKT signaling
R-DME-1632852 Macroautophagy
R-DME-166208 mTORC1-mediated signalling
R-DME-3371571 HSF1-dependent transactivation
R-DME-380972 Energy dependent regulation of mTOR by LKB1-AMPK
R-DME-389357 CD28 dependent PI3K/Akt signaling
R-DME-5218920 VEGFR2 mediated vascular permeability
R-DME-5628897 TP53 Regulates Metabolic Genes
R-DME-8943724 Regulation of PTEN gene transcription
SignaLinkiQ9VK45

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Tor (EC:2.7.11.1)
Alternative name(s):
Target of rapamycin
Gene namesi
Name:Tor
ORF Names:CG5092
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0021796 Tor

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Not lethal. Displays phenotypes characteristic of amino acid deprivation, including reduced nucleolar size, lipid vesicle aggregation in the larval fat body, and a cell type-specific pattern of cell cycle arrest that can be bypassed by overexpression of the S-phase regulator cyclin E. Reach only the size of second instar larvae, at which point they undergo cell cycle arrest.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1956S → T: Rapamycin resistance and loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003774581 – 2470Serine/threonine-protein kinase TorAdd BLAST2470

Proteomic databases

PaxDbiQ9VK45
PRIDEiQ9VK45

Expressioni

Inductioni

By PI3K/Akt signaling, or by nutrients such as amino acids, and by high cellular energy levels.1 Publication

Gene expression databases

BgeeiFBgn0021796
ExpressionAtlasiQ9VK45 differential
GenevisibleiQ9VK45 DM

Interactioni

Subunit structurei

May be part of a minimal complex, TORC1, consisting of tor, raptor and lst8. May be part of a minimal complex, TORC2, consisting of tor, rictor and lst8 (By similarity). Self-associates; assembles into homomultimeric complexes. Component of a multiprotein complex.By similarity1 Publication

GO - Molecular functioni

  • protein self-association Source: FlyBase

Protein-protein interaction databases

BioGridi70789, 44 interactors
IntActiQ9VK45, 16 interactors
STRINGi7227.FBpp0080003

Structurei

3D structure databases

ProteinModelPortaliQ9VK45
SMRiQ9VK45
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati172 – 209HEAT 1Sequence analysisAdd BLAST38
Repeati746 – 785HEAT 2Sequence analysisAdd BLAST40
Repeati791 – 829HEAT 3Sequence analysisAdd BLAST39
Repeati835 – 873HEAT 4Sequence analysisAdd BLAST39
Repeati962 – 999HEAT 5Sequence analysisAdd BLAST38
Repeati1043 – 1080HEAT 6Sequence analysisAdd BLAST38
Repeati1083 – 1122HEAT 7Sequence analysisAdd BLAST40
Repeati1124 – 1160HEAT 8Sequence analysisAdd BLAST37
Domaini1349 – 1903FATPROSITE-ProRule annotationAdd BLAST555
Repeati1407 – 1440TPR 1Sequence analysisAdd BLAST34
Repeati1718 – 1751TPR 2PROSITE-ProRule annotationAdd BLAST34
Repeati1854 – 1891HEAT 9Sequence analysisAdd BLAST38
Domaini2103 – 2470PI3K/PI4KPROSITE-ProRule annotationAdd BLAST368
Domaini2438 – 2470FATCPROSITE-ProRule annotationAdd BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi293 – 297Poly-Ser5

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0891 Eukaryota
COG5032 LUCA
GeneTreeiENSGT00910000144247
InParanoidiQ9VK45
KOiK07203
OMAiSSHQGLM
OrthoDBiEOG091G0046
PhylomeDBiQ9VK45

Family and domain databases

Gene3Di1.10.1070.11, 1 hit
1.20.120.150, 1 hit
1.25.10.10, 3 hits
1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR024585 DUF3385_TOR
IPR003152 FATC_dom
IPR009076 FRB_dom
IPR036738 FRB_sf
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR003151 PIK-rel_kinase_FAT
IPR014009 PIK_FAT
IPR026683 TOR
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PANTHERiPTHR11139:SF9 PTHR11139:SF9, 1 hit
PfamiView protein in Pfam
PF11865 DUF3385, 1 hit
PF02259 FAT, 1 hit
PF02260 FATC, 1 hit
PF08771 FRB_dom, 1 hit
PF00454 PI3_PI4_kinase, 1 hit
SMARTiView protein in SMART
SM01346 DUF3385, 1 hit
SM01343 FATC, 1 hit
SM00146 PI3Kc, 1 hit
SUPFAMiSSF47212 SSF47212, 1 hit
SSF48371 SSF48371, 4 hits
SSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS51189 FAT, 1 hit
PS51190 FATC, 1 hit
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS50005 TPR, 2 hits
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VK45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTSVVQQF VNGLKSRNRN VQNKATQDLL FYVKTELREM SQEELAQFFD
60 70 80 90 100
EFDHHIFTMV NATDINEKKG GALAMKCLIN CEGSLTARKG ISPYLNRLRD
110 120 130 140 150
LLLINDVSVM EIAARSLVKL ANMPTSKGAD SFDFDIKKAF EVLRGERQEY
160 170 180 190 200
RRHSAVFILR ELAIALPTYF YQHILTFFEV IFNAIFDPKP AIRESAGEAL
210 220 230 240 250
RAALIVTAQR ESTKQSSEPQ WYRICYDEAN GSFNADLGSS KDQKGVTRDD
260 270 280 290 300
RIHGGLVVFN ELFRCANATW ERRYTSLKTL FPKTQHNKFL EASSSSSMGS
310 320 330 340 350
QLNTLVPRLK VPFIDKLGST QTHLGEGEHH KGVAKFASHN VLESAYAQEI
360 370 380 390 400
LQEHYTSICD NVLEQRTSKS PYVQQALLQI LPRLAAFNRA VFVEKYLQTC
410 420 430 440 450
VSHLMQILRG KEKDRTVAYI TIGYMAVAVQ SAIEVHLSSI MTSVKVALPS
460 470 480 490 500
KDLTSKRKVP VDPAVFACIT LLAHAVKSEI ADDVKDILEQ MFYTGLSPAL
510 520 530 540 550
TVCLRELSEN VPQLKSAITE GLIGILSQVL MNKAAILPYT ALPTIAIDGS
560 570 580 590 600
LMQNGDGATT VLALKTLGTF NFEEQNMLDF VQRCADYFIV HEQQEIRLEA
610 620 630 640 650
VQTCTRLLKL AVQSSESMEN SKTLSDTVSH VIERLLMVAI TDMDCNVRIR
660 670 680 690 700
ILRSLDETFD GKLAQPESLN SLFITLHDEI FEIRELAMVT IGRLSSINPA
710 720 730 740 750
YVMPKLRTTM IELITDLKYS GMSRNKEQSA KMLDHLVIST PRLISSYMNP
760 770 780 790 800
ILKALVPKLH EPESNPGVIL NVLRTIGDLA EVNGGSDEME LWADDLLSIL
810 820 830 840 850
LEMLGDAGSP DKRGVALWTL GQLISATGRV VTPYHKYPVL IDILINFLKT
860 870 880 890 900
EQRRSIRRET IRVLGLLGAM DPYKHKMNKG LIDSQKDNVL IAYSDGKVDE
910 920 930 940 950
SQDISTAELL VNMGNALDEY YPAVAIAALM RILRDPTLST RHTSVVQAVT
960 970 980 990 1000
FIFQSLGIKC VPYLAQVLPN LLDNVRTADN NLREFLFQQL AILVAFVKLH
1010 1020 1030 1040 1050
IISYMGDIFK LIKEFWTINT PLQNTLINLI EQIAVALGCE FRDYLAELIP
1060 1070 1080 1090 1100
QILRVLQHDN SKDRMVTRRL LQALQKFGST LGYYLPLILP PIVKLFDSPY
1110 1120 1130 1140 1150
VPQQVSMVAL ETINNLACQL DFTDFSSRII HPLVRVLDAE PELRDQAMTT
1160 1170 1180 1190 1200
LRSLAKQLGK KYLVFVPMVQ RTLNKHRIVD PEYEELLSKI KSCSTLADSY
1210 1220 1230 1240 1250
GAGESELRPS RFKNNEPFVT DRNSNNKNLQ VTTNELRTAW QVTRRVSKDD
1260 1270 1280 1290 1300
WVEWLKRLSI GLLKESPSHA LRACRSLAQE YDTLLRDLFN AAFISCWTEL
1310 1320 1330 1340 1350
SPDLKNELTQ SLIQALQVTD MPEITQTILN LAEFMEHCDR DPIPIETKLL
1360 1370 1380 1390 1400
GTRAMACRAY AKALRYKEEE FLLREDSQVF ESLILINNKL QQREAAEGLL
1410 1420 1430 1440 1450
TRYRNAANEL NVQGRWYEKL HNWDEALEHY ERNLKTDSSD LEARLGHMRC
1460 1470 1480 1490 1500
LEALGDWSEL SNVTKHEWEN FGTEAKSRAG PLAAVAAWGL QDWEAMREYV
1510 1520 1530 1540 1550
RCIPEDTQDG SYYRAVLAVH HDDFETAQRL IDETRDLLDT ELTSMAGESY
1560 1570 1580 1590 1600
ERAYGAMVCV QMLAELEEVI QYKLIPERRE PLKTMWWKRL QGGQRLVEDW
1610 1620 1630 1640 1650
RRIIQVHSLV VKPHEDIHTW LKYASLCRKS GSLHLSHKTL VMLLGTDPKL
1660 1670 1680 1690 1700
NPNQPLPCNQ PQVTYAYTKY MAANNQLQEA YEQLTHFVST YSQELSCLPP
1710 1720 1730 1740 1750
EALKQQDQRL MARCYLRMAT WQNKLQDSIR PDAIQGALEC FEKATSYDPN
1760 1770 1780 1790 1800
WYKAWHLWAY MNFKVVQAQK SALDKQQPPG ASMGMTMGSG LDSDLMIIQR
1810 1820 1830 1840 1850
YAVPAVQGFF RSISLIKGNS LQDTLRLLTL WFDYGNHAEV YEALLSGMKL
1860 1870 1880 1890 1900
IEINTWLQVI PQLIARIDTH RQLVGQLIHQ LLMDIGKNHP QALVYPLTVA
1910 1920 1930 1940 1950
SKSASLARRN AAFKILDSMR KHSPTLVEQA VMCSEELIRV AILWHEQWHE
1960 1970 1980 1990 2000
GLEEASRLYF GDRNVKGMFE ILEPLHAMLE RGPQTLKETS FSQAYGRELT
2010 2020 2030 2040 2050
EAYEWSQRYK TSAVVMDLDR AWDIYYHVFQ KISRQLPQLT SLELPYVSPK
2060 2070 2080 2090 2100
LMTCKDLELA VPGSYNPGQE LIRISIIKTN LQVITSKQRP RKLCIRGSNG
2110 2120 2130 2140 2150
KDYMYLLKGH EDLRQDERVM QLFSLVNTLL LDDPDTFRRN LAIQRYAVIP
2160 2170 2180 2190 2200
LSTNSGLIGW VPHCDTLHTL IRDYRDKKKV PLNQEHRTML NFAPDYDHLT
2210 2220 2230 2240 2250
LMQKVEVFEH ALGQTQGDDL AKLLWLKSPS SELWFERRNN YTRSLAVMSM
2260 2270 2280 2290 2300
VGYILGLGDR HPSNLMLDRM SGKILHIDFG DCFEVAMTRE KFPEKIPFRL
2310 2320 2330 2340 2350
TRMLIKAMEV TGIEGTYRRT CESVMLVLRR NKDSLMAVLE AFVYDPLLNW
2360 2370 2380 2390 2400
RLLDVDKKGN DAVAGAGAPG GRGGSGMQDS LSNSVEDSLP MAKSKPYDPT
2410 2420 2430 2440 2450
LQQGGLHNNV ADETNSKASQ VIKRVKCKLT GTDFQTEKSV NEQSQVELLI
2460 2470
QQATNNENLC QCYIGWCPFW
Length:2,470
Mass (Da):281,033
Last modified:May 1, 2000 - v1
Checksum:i5D78D2ECC07C7FF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA Translation: AAF53237.1
RefSeqiNP_524891.1, NM_080152.3
UniGeneiDm.1502

Genome annotation databases

EnsemblMetazoaiFBtr0080422; FBpp0080003; FBgn0021796
GeneIDi47396
KEGGidme:Dmel_CG5092
UCSCiCG5092-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiTOR_DROME
AccessioniPrimary (citable) accession number: Q9VK45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health