Reviewed, UniProtKB/Swiss-Prot Q9VK33 (SMBT_DROME)

Names and origin

Protein names

Recommended name:
    Polycomb protein Sfmbt
Alternative name(s):
    Scm-like with four MBT domain-containing protein 1
    dSfmbt

Gene names

Name:	Sfmbt
ORF Names:	CG16975

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Polycomb group (PcG) protein that binds to the Polycomb response elements (PREs) found in the regulatory regions of many genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Necessary but not sufficient to recruit a functional PcG repressive complex that represses target genes, suggesting that the recruitment of the distinct PRC1 complex is also required to allow a subsequent repression. Ref.4
Subunit structure	Interacts with pho as a component of the pho-repressive complex (PhoRC). Ref.4
Subcellular location	Nucleus. Ref.4
Domain	MBT repeats have unique discriminatory binding activity for methylated Lys residues in H3 and H4; the MBT repeats bind mono- and dimethylated H3K9Me1, H3K9Me2, H4K20Me1 and H4K20Me2 but fail to interact with these residues if they are unmodified or trimethylated. Ref.4
Sequence similarities	Contains 1 FCS-type zinc finger. Contains 4 MBT repeats. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Chromatin regulator Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW chromatin silencing Ref.4 Inferred from direct assay. Source: FlyBase imaginal disc growth Ref.4 Inferred from mutant phenotype. Source: FlyBase oogenesis Ref.4 Inferred from mutant phenotype. Source: FlyBase transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.4 Inferred from direct assay. Source: FlyBase
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW methylated histone residue binding Ref.4 Inferred from direct assay. Source: FlyBase zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VQD0	1	EBI-117801,EBI-138843

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B Ref.1 (identifier: Q9VK33-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A Ref.1 (identifier: Q9VK33-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-352: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1220	1220	Polycomb protein Sfmbt		PRO_0000306371
Regions
	Repeat	536 – 647	112	MBT 1
	Repeat	655 – 753	99	MBT 2
	Repeat	761 – 871	111	MBT 3
	Repeat	879 – 975	97	MBT 4
	Domain	1140 – 1203	64	SAM
	Zinc finger	322 – 357	36	FCS-type
Natural variations
	Alternative sequence	1 – 352	352	Missing in isoform A. Ref.1		VSP_052548

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified June 1, 2003. Version 2. Checksum: 2BE2785144CA051F	FASTA	1,220	133,666
	10 20 30 40 50 60 MNPSELRMMW MSSQYNSERI TLEDAATLLG HPTVGLSVME DLSAHQPTLD MNPMMSLMGG 70 80 90 100 110 120 DFTGQAAATA AALGVQPGTL IATNSNNLYG FAHMGGLQQQ LLQQSAAAAV FQNYAEAMDN 130 140 150 160 170 180 DVENGMVGMA MEAVVDDDDQ VYGQRDNNFD DNGSELEPKQ EIINIDDFVM MNEDNNSYDG 190 200 210 220 230 240 TDFMTSSDKD ISQSSSSCMA QMPGSLGVPG VEHDLLVPLP DGLLHHKLLG TTLVPAMGTL 250 260 270 280 290 300 NGNAFGNIMV STENTSSKQM QRTYSTAKGA NSTATTATCS ASTSSALRSQ RKTRKIEPVN 310 320 330 340 350 360 RPGLVLKTPI AYRGNIDPSV IPIQKDGMAV CKRCGAIGVK HTFYTKSRRF CSMACARGEL 370 380 390 400 410 420 YSLVLNTKME GDQATTSSPD PGAGSESADL PGDQQQSQSD IELDLHAAHI KNANYRFRIT 430 440 450 460 470 480 DQSKITQLNS FGEPMSMGGD AAANNVQMAA DETIAALNGG AVGDATAPGS TEEGASTPNS 490 500 510 520 530 540 YLSAAPTPKA LRLFKDIYPQ DDLPQIPKYE RLPVPCPQME KIISIRRRMY DPTHSYDWLP 550 560 570 580 590 600 RLSKENFNAA PVTCFPHAPG CEVWDNLGVG MKVEVENTDC DSIEVIQPGQ TPTSFWVATI 610 620 630 640 650 660 LEIKGYKALM SYEGFDTDSH DFWVNLCNAE VHSVGWCATR GKPLIPPRTI EHKYKDWKDF 670 680 690 700 710 720 LVGRLSGART LPSNFYNKIN DSLQSRFRLG LNLECVDKDR ISQVRLATVT KIVGKRLFLR 730 740 750 760 770 780 YFDSDDGFWC HEDSPIIHPV GWATTVGHNL AAPQDYLERM LAGREAMIEV HEDDATIELF 790 800 810 820 830 840 KMNFTFDEYY SDGKTNSFVE GMKLEAVDPL NLSSICPATV MAVLKFGYMM IRIDSYQPDA 850 860 870 880 890 900 SGSDWFCYHE KSPCIFPAGF CSVNNISVTP PNGYDSRTFT WEGYLRDTGA VAAGQHLFHR 910 920 930 940 950 960 IIPDHGFEVG MSLECADLMD PRLVCVATVA RVVGRLLKVH FDGWTDEYDQ WLDCESADIY 970 980 990 1000 1010 1020 PVGWCVLVNH KLEGPPRVAH QQAPKPAPKP KIQRKRKPKK GAAGGKTPTD NNTQSVKSRT 1030 1040 1050 1060 1070 1080 IALKTTPHLP KLSIKLELKP EHHNAAFYEN NQPEEEGDEE DPDADGDGDG STSHISEQST 1090 1100 1110 1120 1130 1140 TQSSSDLIAG SGSGSGSASL VTLATGSNKT NSSATNNKYI PRLADIDSSE PHLELVPDTW 1150 1160 1170 1180 1190 1200 NVYDVSQFLR VNDCTAHCDT FSRNKIDGKR LLQLTKDDIM PLLGMKVGPA LKISDLIAQL 1210 1220 KCKVNPGRAR SHKTNKSPFL
	Isoform A. Checksum: A8D56440315A78E4	FASTA	868	95,867
	10 20 30 40 50 60 MACARGELYS LVLNTKMEGD QATTSSPDPG AGSESADLPG DQQQSQSDIE LDLHAAHIKN 70 80 90 100 110 120 ANYRFRITDQ SKITQLNSFG EPMSMGGDAA ANNVQMAADE TIAALNGGAV GDATAPGSTE 130 140 150 160 170 180 EGASTPNSYL SAAPTPKALR LFKDIYPQDD LPQIPKYERL PVPCPQMEKI ISIRRRMYDP 190 200 210 220 230 240 THSYDWLPRL SKENFNAAPV TCFPHAPGCE VWDNLGVGMK VEVENTDCDS IEVIQPGQTP 250 260 270 280 290 300 TSFWVATILE IKGYKALMSY EGFDTDSHDF WVNLCNAEVH SVGWCATRGK PLIPPRTIEH 310 320 330 340 350 360 KYKDWKDFLV GRLSGARTLP SNFYNKINDS LQSRFRLGLN LECVDKDRIS QVRLATVTKI 370 380 390 400 410 420 VGKRLFLRYF DSDDGFWCHE DSPIIHPVGW ATTVGHNLAA PQDYLERMLA GREAMIEVHE 430 440 450 460 470 480 DDATIELFKM NFTFDEYYSD GKTNSFVEGM KLEAVDPLNL SSICPATVMA VLKFGYMMIR 490 500 510 520 530 540 IDSYQPDASG SDWFCYHEKS PCIFPAGFCS VNNISVTPPN GYDSRTFTWE GYLRDTGAVA 550 560 570 580 590 600 AGQHLFHRII PDHGFEVGMS LECADLMDPR LVCVATVARV VGRLLKVHFD GWTDEYDQWL 610 620 630 640 650 660 DCESADIYPV GWCVLVNHKL EGPPRVAHQQ APKPAPKPKI QRKRKPKKGA AGGKTPTDNN 670 680 690 700 710 720 TQSVKSRTIA LKTTPHLPKL SIKLELKPEH HNAAFYENNQ PEEEGDEEDP DADGDGDGST 730 740 750 760 770 780 SHISEQSTTQ SSSDLIAGSG SGSGSASLVT LATGSNKTNS SATNNKYIPR LADIDSSEPH 790 800 810 820 830 840 LELVPDTWNV YDVSQFLRVN DCTAHCDTFS RNKIDGKRLL QLTKDDIMPL LGMKVGPALK 850 860 ISDLIAQLKC KVNPGRARSH KTNKSPFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Embryo.
[4]	"A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities." Klymenko T., Papp B., Fischle W., Koecher T., Schelder M., Fritsch C., Wild B., Wilm M., Mueller J. Genes Dev. 20:1110-1122(2006) [PubMed: 16618800] [Abstract] Cited for: FUNCTION, INTERACTION WITH PHO, SUBCELLULAR LOCATION. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014134 Genomic DNA. Translation: AAF53249.2. AE014134 Genomic DNA. Translation: AAF53250.2. BT006011 mRNA. Translation: AAO74694.1.
RefSeq	NP_609606.2. NP_723786.1.
UniGene	Dm.11543
3D structure databases
HSSP	HSSP built from PDB template 1OI1 based on UniProtKB Q9UQR0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VK33. 8 interactions.
Genome annotation databases
Ensembl	FBgn0032475. Drosophila melanogaster. [Contig view]
GeneID	34709.
KEGG	dme:Dmel_CG16975.
Organism-specific databases
FlyBase	FBgn0032475. Sfmbt.
Phylogenomic databases
HOGENOM	Q9VK33.
OMA	Q9VK33. HEKSPCI.
Gene expression databases
ArrayExpress	Q9VK33.
Family and domain databases
InterPro	IPR004092. Mbt. IPR001660. SAM. IPR013761. SAM_type. IPR012313. Znf_FCS. [Graphical view]
Gene3D	G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF02820. MBT. 4 hits. PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00561. MBT. 4 hits. SM00454. SAM. 1 hit. [Graphical view]
PROSITE	PS51079. MBT. 4 hits. PS50105. SAM_DOMAIN. 1 hit. PS51024. ZF_FCS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	789812.

Entry information

Entry name

SMBT_DROME

Accession

Primary (citable) accession number: Q9VK33
Secondary accession number(s): Q7KTB5, Q9VK32

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project