Reviewed, UniProtKB/Swiss-Prot Q9VJQ5 (NC2B_DROME)

Names and origin

Protein names

Recommended name:
    Protein Dr1
Alternative name(s):
    Down-regulator of transcription 1
    Negative cofactor 2-beta
      Short name=NC2-beta
    dNC2

Gene names

Name:	NC2beta
Synonyms:	Dr1
ORF Names:	CG4185

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Bifunctional basic transcription factor. Activates transcription of DPE (Downstream Promoter Element) containing promoters while repressing transcription of promoters which contain TATA elements. Ref.1
Subunit structure	Interacts with NC2-alpha/Drap1 to form the dNC2 complex. Ref.1
Subcellular location	Nucleus. Ref.1
Sequence similarities	Belongs to the NC2 beta/DR1 family. Contains 1 histone-fold domain.
Sequence caution	The sequence AAL28265.2 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of transcription from RNA polymerase II promoter Ref.1 Inferred from direct assay. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Ref.1 Inferred from direct assay. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	RNA polymerase II transcription factor activity Ref.1 Inferred from direct assay. Source: UniProtKB protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription activator activity Ref.1 Inferred from direct assay. Source: UniProtKB transcription repressor activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Mes4	Q9W256	1	EBI-176193,EBI-156665
NC2alpha	Q9W2E3	1	EBI-176193,EBI-99385
Taf11	P49906	1	EBI-176193,EBI-187533

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	Protein Dr1		PRO_0000096752
Regions
	Region	92 – 183	92	Repression of TATA-containing promoters
	Compositional bias	174 – 182	9	Poly-Asp

Sequences

	Sequence		Length	Mass (Da)
	Q9VJQ5-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BE7B25679CE02F7C	FASTA	183	20,914
	10 20 30 40 50 60 MSNPQEELLP PSAEDDELTL PRASINKIIK ELVPTVRVAN ESRELILNCC SEFIHLISSE 70 80 90 100 110 120 ANEVCNMRNK KTINAEHVLE ALERLGFHDY KQEAEAVLHD CKEVAAKRRR QSTRLENLGI 130 140 150 160 170 180 PEEELLRQQQ ELFAKAREEQ AREEQQQWMS MQAAAMVQRP PLADGSVASK PSEDDDDDDD DDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A basal transcription factor that activates or represses transcription." Willy P.J., Kobayashi R., Kadonaga J.T. Science 290:982-985(2000) [PubMed: 11062130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 72-84 AND 114-133, FUNCTION, INTERACTION WITH NC2-ALPHA, SUBCELLULAR LOCATION. Strain: Berkeley. Tissue: Head.
[2]	"An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region." Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T., Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A., Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R. , Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K., Celniker S.E., Rubin G.M. Genetics 153:179-219(1999) [PubMed: 10471707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[6]	"Intragenic Hill-Robertson interference influences selection intensity on synonymous mutations in Drosophila." Comeron J.M., Guthrie T.B. Mol. Biol. Evol. 22:2519-2530(2005) [PubMed: 16120803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-177. Strain: Ral1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF302257 mRNA. Translation: AAG15388.1. AE014134 Genomic DNA. Translation: AAF53428.1. AY060717 mRNA. Translation: AAL28265.2. Sequence problems. DQ138728 Genomic DNA. Translation: ABA86334.1.
RefSeq	NP_609736.1.
UniGene	Dm.36385
3D structure databases
HSSP	HSSP built from PDB template 1JFI based on UniProtKB Q01658.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VJQ5. 7 interactions.
Proteomic databases
PRIDE	Q9VJQ5.
Genome annotation databases
Ensembl	FBgn0028926. Drosophila melanogaster. [Contig view]
GeneID	34875.
KEGG	dme:Dmel_CG4185.
NMPDR	fig|7227.3.peg.2462.
Organism-specific databases
FlyBase	FBgn0028926. NC2beta.
Phylogenomic databases
HOGENOM	Q9VJQ5.
OMA	Q9VJQ5. KATVQKI.
Gene expression databases
GermOnline	CG4185. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003958. CBFA_NFYB_domain. IPR009072. Histone-fold. [Graphical view]
Gene3D	G3DSA:1.10.20.10. Histone-fold. 1 hit.
Pfam	PF00808. CBFD_NFYB_HMF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	790647.

Entry information

Entry name

NC2B_DROME

Accession

Primary (citable) accession number: Q9VJQ5
Secondary accession number(s): Q2XYD4, Q9NKA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project