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Protein

Restin homolog

Gene

CLIP-190

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together CLIP-190 and jar may coordinate the interaction between the actin and microtubule cytoskeleton. May link endocytic vesicles to microtubules. May play a role in formation of furrows during cellularization.2 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • microtubule binding Source: UniProtKB
  • microtubule plus-end binding Source: FlyBase
  • myosin VI heavy chain binding Source: FlyBase

GO - Biological processi

  • cellularization Source: UniProtKB
  • microtubule-based process Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Restin homolog
Alternative name(s):
Cytoplasmic linker protein 190
Microtubule-binding protein 190
d-CLIP-190
Gene namesi
ORF Names:CG5020
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0020503. CLIP-190.

Subcellular locationi

  • Cytoplasm Curated
  • Cytoplasmcytoskeleton 1 Publication
  • Golgi apparatus 1 Publication

  • Note: Microtubule-associated. Lva-CLIP-190 complexes are found at the Golgi.2 Publications

GO - Cellular componenti

  • axon Source: FlyBase
  • axonal growth cone Source: FlyBase
  • fusome Source: FlyBase
  • Golgi apparatus Source: UniProtKB
  • kinetochore Source: FlyBase
  • microtubule associated complex Source: UniProtKB
  • microtubule plus-end Source: FlyBase
  • spindle microtubule Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16901690Restin homologPRO_0000083514Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641Phosphoserine1 Publication
Modified residuei67 – 671Phosphoserine1 Publication
Modified residuei216 – 2161Phosphoserine1 Publication
Modified residuei309 – 3091Phosphoserine1 Publication
Modified residuei322 – 3221Phosphoserine1 Publication
Modified residuei325 – 3251Phosphoserine1 Publication
Modified residuei327 – 3271Phosphothreonine1 Publication
Modified residuei328 – 3281Phosphoserine1 Publication
Modified residuei362 – 3621Phosphothreonine1 Publication
Modified residuei1681 – 16811Phosphothreonine1 Publication
Modified residuei1682 – 16821Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VJE5.
PRIDEiQ9VJE5.

PTM databases

iPTMnetiQ9VJE5.

Expressioni

Tissue specificityi

Specifically expressed at the tip of the furrow in cellularizing blastoderms. CLIP-190 and jar are coexpressed at several times in development and in a number of tissues, including embryonic axonal neuron processes and posterior pole.2 Publications

Gene expression databases

BgeeiFBgn0020503.
ExpressionAtlasiQ9VJE5. differential.
GenevisibleiQ9VJE5. DM.

Interactioni

Subunit structurei

Interacts with Lva.1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • microtubule binding Source: UniProtKB
  • microtubule plus-end binding Source: FlyBase
  • myosin VI heavy chain binding Source: FlyBase

Protein-protein interaction databases

BioGridi61038. 6 interactions.
DIPiDIP-21005N.
IntActiQ9VJE5. 18 interactions.
MINTiMINT-764195.
STRINGi7227.FBpp0080529.

Structurei

3D structure databases

ProteinModelPortaliQ9VJE5.
SMRiQ9VJE5. Positions 52-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini143 – 18543CAP-Gly 1PROSITE-ProRule annotationAdd
BLAST
Domaini260 – 30243CAP-Gly 2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili378 – 46891Sequence analysisAdd
BLAST
Coiled coili484 – 660177Sequence analysisAdd
BLAST
Coiled coili667 – 916250Sequence analysisAdd
BLAST
Coiled coili926 – 98156Sequence analysisAdd
BLAST
Coiled coili1001 – 1121121Sequence analysisAdd
BLAST
Coiled coili1158 – 1549392Sequence analysisAdd
BLAST
Coiled coili1565 – 160036Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi488 – 1452965Glu-richAdd
BLAST
Compositional biasi1200 – 1461262Gln-richAdd
BLAST

Sequence similaritiesi

Contains 2 CAP-Gly domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410ISTI. Eukaryota.
ENOG410XSDG. LUCA.
GeneTreeiENSGT00760000119173.
InParanoidiQ9VJE5.
OMAiSKADEWS.
OrthoDBiEOG091G0J1Y.
PhylomeDBiQ9VJE5.

Family and domain databases

Gene3Di2.30.30.190. 2 hits.
InterProiIPR000938. CAP-Gly_domain.
IPR032108. CLIP1_ZNF.
[Graphical view]
PfamiPF01302. CAP_GLY. 2 hits.
PF16641. CLIP1_ZNF. 2 hits.
[Graphical view]
SMARTiSM01052. CAP_GLY. 2 hits.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 2 hits.
PROSITEiPS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q9VJE5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDDTSASGG TSAPFPSPVT ADPEPGATAS KLPGPIRSNI PTPATSGTGI
60 70 80 90 100
PQPSKMKAPS SFGSTGSVSK IGRPCCNHTT PKSGPPPREA TSMSRESDDN
110 120 130 140 150
LSSINSAYTD NSSAVLTANT EQFIIGQRVW LGGTRPGQIA FIGDTHFAAG
160 170 180 190 200
EWAGVVLDEP NGKNDGCVSG KRYFQCEPKR GIFSRLTRLT TYPLAGAQTP
210 220 230 240 250
TSPLAKSSPD RSRTVSPTAS IRSSMLRSPG IGGKNGMAVG DRVIVSSGFG
260 270 280 290 300
SRPGILRYLG ETQFAPGNWC GVELDEPSGK NDGTVDDIRY FECKPKYGVF
310 320 330 340 350
VPIAKVSLSP SSKKTRLSRT GSRESLTSIG TMNSIATTAT SRMRMNAQQR
360 370 380 390 400
KSSTPVKPIL ATPKSQFSMQ DLLREKQQHV EKLMVERDLD REDAQNQALQ
410 420 430 440 450
LQKNINELKA RIVELESALD NERKKTEELQ CSIDEAQFCG DELNAQSQVY
460 470 480 490 500
KEKIHDLESK ITKLVSATPS LQSILPPDLP SDDGALQEEI AKLQEKMTIQ
510 520 530 540 550
QKEVESRIAE QLEEEQRLRE NVKYLNEQIA TLQSELVSKD EALEKFSLSE
560 570 580 590 600
CGIENLRREL ELLKEENEKQ AQEAQAEFTR KLAEKSVEVL RLSSELQNLK
610 620 630 640 650
ATSDSLESER VNKTDECEIL QTEVRMRDEQ IRELNQQLDE VTTQLNVQKA
660 670 680 690 700
DSSALDDMLR LQKEGTEEKS TLLEKTEKEL VQSKEQAAKT LNDKEQLEKQ
710 720 730 740 750
ISDLKQLAEQ EKLVREMTEN AINQIQLEKE SIEQQLALKQ NELEDFQKKQ
760 770 780 790 800
SESEVHLQEI KAQNTQKDFE LVESGESLKK LQQQLEQKTL GHEKLQAALE
810 820 830 840 850
ELKKEKETII KEKEQELQQL QSKSAESESA LKVVQVQLEQ LQQQAAASGE
860 870 880 890 900
EGSKTVAKLH DEISQLKSQA EETQSELKST QSNLEAKSKQ LEAANGSLEE
910 920 930 940 950
EAKKSGHLLE QITKLKSEVG ETQAALSSCH TDVESKTKQL EAANAALEKV
960 970 980 990 1000
NKEYAESRAE ASDLQDKVKE ITDTLHAELQ AERSSSSALH TKLSKFSDEI
1010 1020 1030 1040 1050
ATGHKELTSK ADAWSQEMLQ KEKELQELRQ QLQDSQDSQT KLKAEGERKE
1060 1070 1080 1090 1100
KSFEESIKNL QEEVTKAKTE NLELSTGTQT TIKDLQERLE ITNAELQHKE
1110 1120 1130 1140 1150
KMASEDAQKI ADLKTLVEAI QVANANISAT NAELSTVLEV LQAEKSETNH
1160 1170 1180 1190 1200
IFELFEMEAD MNSERLIEKV TGIKEELKET HLQLDERQKK FEELEEKLKQ
1210 1220 1230 1240 1250
AQQSEQKLQQ ESQTSKEKLT EIQQSLQELQ DSVKQKEELV QNLEEKVRES
1260 1270 1280 1290 1300
SSIIEAQNTK LNESNVQLEN KTSCLKETQD QLLESQKKEK QLQEEAAKLS
1310 1320 1330 1340 1350
GELQQVQEAN GDIKDSLVKV EELVKVLEEK LQAATSQLDA QQATNKELQE
1360 1370 1380 1390 1400
LLVKSQENEG NLQGESLAVT EKLQQLEQAN GELKEALCQK ENGLKELQGK
1410 1420 1430 1440 1450
LDESNTVLES QKKSHNEIQD KLEQAQQKER TLQEETSKLA EQLSQLKQAN
1460 1470 1480 1490 1500
EELQKSLQQK QLLLEKGNEF DTQLAEYQKV IDEMDDAASV KSALLEQLQN
1510 1520 1530 1540 1550
RVAELETALR QANDAQKTAY LETKELRRQL ESLELEKSRE VLSLKAQMNG
1560 1570 1580 1590 1600
ASSRSGKGDE VESLDIETSL AKINFLNSII ADMQQKNDAL KAKVQTLETL
1610 1620 1630 1640 1650
PMDFTKPHAF DALTKRKPAP RLFCDICDEF DQHDTEDCPI QGSEDQDYST
1660 1670 1680 1690
PSSESNNNEK ERKLPAPRKY CDSCEVFGHD TSECADDETY
Length:1,690
Mass (Da):189,065
Last modified:May 1, 2000 - v1
Checksum:iD6F7916A9C532F16
GO
Isoform B (identifier: Q9VJE5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     348-348: Missing.

Note: No experimental confirmation available.
Show »
Length:1,689
Mass (Da):188,937
Checksum:i410773DA3899E2E1
GO
Isoform C (identifier: Q9VJE5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-109: MSDDTSASGG...NLSSINSAYT → MSRESDDNLS...LEATGRRRSS

Note: No experimental confirmation available.
Show »
Length:1,652
Mass (Da):186,352
Checksum:iB47CB31029196839
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091T → I in AAR82803 (Ref. 5) Curated
Sequence conflicti207 – 2071S → N in AAB96783 (PubMed:9472041).Curated
Sequence conflicti420 – 4201D → G in AAB96783 (PubMed:9472041).Curated
Sequence conflicti420 – 4201D → G in AAR82803 (Ref. 5) Curated
Sequence conflicti492 – 4921K → Q in AAB96783 (PubMed:9472041).Curated
Sequence conflicti492 – 4921K → Q in AAR82803 (Ref. 5) Curated
Sequence conflicti561 – 5611E → A in AAB96783 (PubMed:9472041).Curated
Sequence conflicti614 – 6141T → S in AAB96783 (PubMed:9472041).Curated
Sequence conflicti683 – 6831S → I in AAB96783 (PubMed:9472041).Curated
Sequence conflicti683 – 6831S → I in AAR82803 (Ref. 5) Curated
Sequence conflicti692 – 6921N → Q in AAB96783 (PubMed:9472041).Curated
Sequence conflicti692 – 6921N → Q in AAR82803 (Ref. 5) Curated
Sequence conflicti717 – 7171M → K in AAB96783 (PubMed:9472041).Curated
Sequence conflicti717 – 7171M → K in AAR82803 (Ref. 5) Curated
Sequence conflicti728 – 7281E → D in AAR82803 (Ref. 5) Curated
Sequence conflicti745 – 7451D → V in AAR82803 (Ref. 5) Curated
Sequence conflicti769 – 7691F → L in AAB96783 (PubMed:9472041).Curated
Sequence conflicti769 – 7691F → L in AAR82803 (Ref. 5) Curated
Sequence conflicti787 – 7871Q → E in AAB96783 (PubMed:9472041).Curated
Sequence conflicti787 – 7871Q → E in AAR82803 (Ref. 5) Curated
Sequence conflicti805 – 8073EKE → KKK in AAR82803 (Ref. 5) Curated
Sequence conflicti881 – 8811Q → E in AAB96783 (PubMed:9472041).Curated
Sequence conflicti907 – 9093HLL → QLQ in AAB96783 (PubMed:9472041).Curated
Sequence conflicti920 – 9201G → E in AAB96783 (PubMed:9472041).Curated
Sequence conflicti929 – 9291C → Y in AAB96783 (PubMed:9472041).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 109109MSDDT…NSAYT → MSRESDDNLSSINSAYTDLY QETVRRFTRSSLSPTPDWDR FSPARRSLKSEAGSRASYDY YLEATGRRRSS in isoform C. CuratedVSP_050480Add
BLAST
Alternative sequencei348 – 3481Missing in isoform B. 2 PublicationsVSP_050479

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041382 mRNA. Translation: AAB96783.1.
AE014134 Genomic DNA. Translation: AAF53604.1.
AE014134 Genomic DNA. Translation: AAF53605.2.
AE014134 Genomic DNA. Translation: AAN10987.1.
AY118896 mRNA. Translation: AAM50756.1.
BT011136 mRNA. Translation: AAR82803.1.
PIRiT13030.
RefSeqiNP_609835.2. NM_135991.4. [Q9VJE5-1]
NP_724047.2. NM_165213.5. [Q9VJE5-2]
NP_724048.1. NM_165214.2. [Q9VJE5-3]
UniGeneiDm.3487.

Genome annotation databases

EnsemblMetazoaiFBtr0080976; FBpp0080529; FBgn0020503. [Q9VJE5-1]
GeneIDi35042.
KEGGidme:Dmel_CG5020.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041382 mRNA. Translation: AAB96783.1.
AE014134 Genomic DNA. Translation: AAF53604.1.
AE014134 Genomic DNA. Translation: AAF53605.2.
AE014134 Genomic DNA. Translation: AAN10987.1.
AY118896 mRNA. Translation: AAM50756.1.
BT011136 mRNA. Translation: AAR82803.1.
PIRiT13030.
RefSeqiNP_609835.2. NM_135991.4. [Q9VJE5-1]
NP_724047.2. NM_165213.5. [Q9VJE5-2]
NP_724048.1. NM_165214.2. [Q9VJE5-3]
UniGeneiDm.3487.

3D structure databases

ProteinModelPortaliQ9VJE5.
SMRiQ9VJE5. Positions 52-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61038. 6 interactions.
DIPiDIP-21005N.
IntActiQ9VJE5. 18 interactions.
MINTiMINT-764195.
STRINGi7227.FBpp0080529.

PTM databases

iPTMnetiQ9VJE5.

Proteomic databases

PaxDbiQ9VJE5.
PRIDEiQ9VJE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080976; FBpp0080529; FBgn0020503. [Q9VJE5-1]
GeneIDi35042.
KEGGidme:Dmel_CG5020.

Organism-specific databases

CTDi35042.
FlyBaseiFBgn0020503. CLIP-190.

Phylogenomic databases

eggNOGiENOG410ISTI. Eukaryota.
ENOG410XSDG. LUCA.
GeneTreeiENSGT00760000119173.
InParanoidiQ9VJE5.
OMAiSKADEWS.
OrthoDBiEOG091G0J1Y.
PhylomeDBiQ9VJE5.

Miscellaneous databases

GenomeRNAii35042.
PROiQ9VJE5.

Gene expression databases

BgeeiFBgn0020503.
ExpressionAtlasiQ9VJE5. differential.
GenevisibleiQ9VJE5. DM.

Family and domain databases

Gene3Di2.30.30.190. 2 hits.
InterProiIPR000938. CAP-Gly_domain.
IPR032108. CLIP1_ZNF.
[Graphical view]
PfamiPF01302. CAP_GLY. 2 hits.
PF16641. CLIP1_ZNF. 2 hits.
[Graphical view]
SMARTiSM01052. CAP_GLY. 2 hits.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 2 hits.
PROSITEiPS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCL190_DROME
AccessioniPrimary (citable) accession number: Q9VJE5
Secondary accession number(s): O44929, Q8INY8, Q8MSD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.