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Protein

Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11

Gene

Pde11

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.1 Publication

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Kineticsi

  1. KM=18.5 µM for cAMP KM=6 µM for cGMP1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei860 – 8601Proton donorBy similarity
    Metal bindingi864 – 8641Divalent metal cation 1By similarity
    Metal bindingi900 – 9001Divalent metal cation 1By similarity
    Metal bindingi901 – 9011Divalent metal cation 1By similarity
    Metal bindingi901 – 9011Divalent metal cation 2By similarity
    Metal bindingi904 – 9041Divalent metal cation 2By similarity
    Metal bindingi929 – 9291Divalent metal cation 2By similarity
    Metal bindingi1011 – 10111Divalent metal cation 1By similarity
    Binding sitei1064 – 10641cAMP or cGMPBy similarity

    GO - Molecular functioni

    • 3',5'-cyclic-AMP phosphodiesterase activity Source: UniProtKB
    • 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • cAMP metabolic process Source: UniProtKB
    • cGMP metabolic process Source: UniProtKB
    • lateral inhibition Source: FlyBase
    • signal transduction Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_325910. cGMP effects.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11 (EC:3.1.4.17, EC:3.1.4.35)
    Alternative name(s):
    cAMP and cGMP phosphodiesterase 11
    Gene namesi
    Name:Pde11
    ORF Names:CG34341
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803 Componenti: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0085370. Pde11.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: FlyBase
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14511451Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11PRO_0000247044Add
    BLAST

    Proteomic databases

    PaxDbiQ9VJ79.

    Expressioni

    Tissue specificityi

    In adults, it is enriched in Malpighian tubules.1 Publication

    Gene expression databases

    BgeeiQ9VJ79.
    ExpressionAtlasiQ9VJ79. differential.
    GenevisibleiQ9VJ79. DM.

    Interactioni

    Protein-protein interaction databases

    BioGridi61102. 6 interactions.
    MINTiMINT-1027877.
    STRINGi7227.FBpp0111456.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VJ79.
    SMRiQ9VJ79. Positions 402-1137.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini419 – 572154GAF 1Add
    BLAST
    Domaini604 – 754151GAF 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni836 – 1100265CatalyticBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1121 – 11244Poly-Gln
    Compositional biasi1328 – 1436109His-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG270709.
    InParanoidiQ9VJ79.
    KOiK13298.
    OMAiFNCEIDS.
    OrthoDBiEOG7RRF69.
    PhylomeDBiQ9VJ79.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 2 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 2 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform B (identifier: Q9VJ79-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MGQAASMCRF RGCRYKNKNK SSKQQQQQQQ QQQQQQQQHQ QQQQQTPSHS
    60 70 80 90 100
    PQIQHHSEII PATTGLHLRS IEEPATTPLQ FQPTGRMNTE QGGTGYGGYG
    110 120 130 140 150
    SSEHSLLIAT RHAGVPLPLA QHQPLPAHYQ PLNHSGAAPP SSSNGSSSSG
    160 170 180 190 200
    GGVQTSATPQ QQQQYQVQQP YQYQYQHHYH HQANSPQHHR PYDPEHARME
    210 220 230 240 250
    AWLDENQEFV QDYFIRKATR QTVDAWLVSH ATSAGNDVVS STSPTHANGQ
    260 270 280 290 300
    TSSSRGGSGA TTPVRKISAH EFERGGLLKP IVNTIDGTPT FLSIGPPMDN
    310 320 330 340 350
    GSVGGSCSNL QNVGGVVAGQ YQYNHQQHHH NHAHLHHSQH SHYQAGGAVG
    360 370 380 390 400
    SSSLGSTGGA SGAGGAPSLG GSGGAGNGHQ YPYYHCHQRP QRLSRNELKQ
    410 420 430 440 450
    LDEKELIFEL VKDICNELEV RTLCHKILQN VSILLNADRG SLFLVQGRCN
    460 470 480 490 500
    GPDGLKKCLV SKLFDVCPRS TVEEMEQQDE VRVAWGTGIA GHVAESGEPV
    510 520 530 540 550
    NIPDAYQDER FNCEIDSLTG YRTKALLCMP IKDSSGDVIG VAQVINKMNG
    560 570 580 590 600
    ECFSEIDEKV FSSYLQFCGI GLRNAQLYEK SQLEIKRNQV LLDLARMIFE
    610 620 630 640 650
    EQSTIEHMVF RILTHMQSLI QCQRVQILLV HEADKGSFSR VFDFEANDLS
    660 670 680 690 700
    EEEATSRTSP YESRFPINIG ITGHVATTGE TVNVPNAYED DRFDASVDEN
    710 720 730 740 750
    SCFKHRSILC MAIKNSLGQI IGVIQLINKF NELDFTKNDE NFVEAFAIFC
    760 770 780 790 800
    GMGIHNTHMY EKAIVAMAKQ SVTLEVLSYH ASATMDEAHR LRRLRVPSAV
    810 820 830 840 850
    HFRLHDFKFD DIHFEDDDTL KACLRMFLDL DFVERFHIDY EVLCRWLLSV
    860 870 880 890 900
    KKNYRNVTYH NWRHAFNVAQ MMFAILTTTQ WWKIFGEIEC LALIIGCLCH
    910 920 930 940 950
    DLDHRGTNNS FQIKASSPLA QLYSTSTMEH HHFDQCLMIL NSPGNQILAN
    960 970 980 990 1000
    LSSDDYCRVI RVLEDAILST DLAVYFKKRG PFLESVSQPT SYWVAEEPRA
    1010 1020 1030 1040 1050
    LLRAMSMTVC DLSAITKPWE IEKRVADLVS SEFFEQGDME KQELNITPID
    1060 1070 1080 1090 1100
    IMNREKEDEL PMMQVNFIDS ICLPIYEAFA TLSDKLEPLV EGVRDNRGHW
    1110 1120 1130 1140 1150
    IDLADVVKTK TSQDQEPEEE QQQQNVISNG DCKAMSDDDV AASEAEVAVD
    1160 1170 1180 1190 1200
    SPSEKASVNG SNVANNSSNT NKKIAVASHP TSTQPSDDDN DVDADADDVD
    1210 1220 1230 1240 1250
    EQAAEENGHD AEVDEASCRS NSTCSSSTAS SCLSTPPPTG EDDSTPVSPL
    1260 1270 1280 1290 1300
    KTLQAKLVAA NLNALQRQTS NQAQTQKQRC KSCDHSRSGL QVRKTSSLRG
    1310 1320 1330 1340 1350
    AQELDLDSKT RNGTHAALCK STPVINNHSH HHNHSHSHNH NHHHHHHHHS
    1360 1370 1380 1390 1400
    HHNHSQHGIG IGSASIGGSG LISLTTPLLA MDSDRIPKIV GKIGNLDGLP
    1410 1420 1430 1440 1450
    FANGIGGPQN GHGLPFGSYQ HHHHHQHHHH LLARRHSETN SNGATAMAVE

    K
    Note: No experimental confirmation available.
    Length:1,451
    Mass (Da):160,930
    Last modified:January 15, 2008 - v4
    Checksum:iDFEC01058FB600A9
    GO
    Isoform C (identifier: Q9VJ79-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-44: Missing.
         45-64: QTPSHSPQIQHHSEIIPATT → MASSPNNAAPPVLWRARRKI

    Note: No experimental confirmation available.
    Show »
    Length:1,407
    Mass (Da):155,622
    Checksum:iDC01CA40E8325C1E
    GO
    Isoform D (identifier: Q9VJ79-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MKVTQSEENT...GLTFALLAGP

    Note: No experimental confirmation available.
    Show »
    Length:1,539
    Mass (Da):170,998
    Checksum:iE1EBAD64C5391214
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 381Q → QQQ in AAM52774 (PubMed:12537569).Curated
    Sequence conflicti1192 – 11921V → A in AAM52774 (PubMed:12537569).Curated
    Sequence conflicti1196 – 11961A → V in AAM52774 (PubMed:12537569).Curated
    Sequence conflicti1232 – 12321C → R in AAM52774 (PubMed:12537569).Curated
    Sequence conflicti1342 – 13421H → HNH in AAM52774 (PubMed:12537569).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4444Missing in isoform C. CuratedVSP_030328Add
    BLAST
    Alternative sequencei1 – 11M → MKVTQSEENTRNTSDRSKSV QTNRKFDNFNWLLSCGLLAA VKSTKLIPQLPRQQQPKKYN LRYAAELLKFDKIQFIKTEG LTFALLAGP in isoform D. 1 PublicationVSP_019906
    Alternative sequencei45 – 6420QTPSH…IPATT → MASSPNNAAPPVLWRARRKI in isoform C. CuratedVSP_030329Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE014134 Genomic DNA. Translation: AAF53675.3.
    AE014134 Genomic DNA. Translation: AAF53676.2.
    AY122262 mRNA. Translation: AAM52774.1.
    RefSeqiNP_001097177.1. NM_001103707.2. [Q9VJ79-3]
    NP_001286044.1. NM_001299115.1. [Q9VJ79-1]
    NP_001286045.1. NM_001299116.1. [Q9VJ79-3]
    NP_609885.2. NM_136041.2. [Q9VJ79-1]
    UniGeneiDm.13948.

    Genome annotation databases

    EnsemblMetazoaiFBtr0112544; FBpp0111456; FBgn0085370. [Q9VJ79-1]
    FBtr0343830; FBpp0310380; FBgn0085370. [Q9VJ79-1]
    GeneIDi35107.
    KEGGidme:Dmel_CG34341.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE014134 Genomic DNA. Translation: AAF53675.3.
    AE014134 Genomic DNA. Translation: AAF53676.2.
    AY122262 mRNA. Translation: AAM52774.1.
    RefSeqiNP_001097177.1. NM_001103707.2. [Q9VJ79-3]
    NP_001286044.1. NM_001299115.1. [Q9VJ79-1]
    NP_001286045.1. NM_001299116.1. [Q9VJ79-3]
    NP_609885.2. NM_136041.2. [Q9VJ79-1]
    UniGeneiDm.13948.

    3D structure databases

    ProteinModelPortaliQ9VJ79.
    SMRiQ9VJ79. Positions 402-1137.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi61102. 6 interactions.
    MINTiMINT-1027877.
    STRINGi7227.FBpp0111456.

    Proteomic databases

    PaxDbiQ9VJ79.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblMetazoaiFBtr0112544; FBpp0111456; FBgn0085370. [Q9VJ79-1]
    FBtr0343830; FBpp0310380; FBgn0085370. [Q9VJ79-1]
    GeneIDi35107.
    KEGGidme:Dmel_CG34341.

    Organism-specific databases

    CTDi35107.
    FlyBaseiFBgn0085370. Pde11.

    Phylogenomic databases

    eggNOGiNOG270709.
    InParanoidiQ9VJ79.
    KOiK13298.
    OMAiFNCEIDS.
    OrthoDBiEOG7RRF69.
    PhylomeDBiQ9VJ79.

    Enzyme and pathway databases

    ReactomeiREACT_325910. cGMP effects.

    Miscellaneous databases

    GenomeRNAii35107.
    NextBioi791899.
    PROiQ9VJ79.

    Gene expression databases

    BgeeiQ9VJ79.
    ExpressionAtlasiQ9VJ79. differential.
    GenevisibleiQ9VJ79. DM.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 2 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 2 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
      Strain: Berkeley.
      Tissue: Embryo.
    4. "Cyclic nucleotide phosphodiesterases in Drosophila melanogaster."
      Day J.P., Dow J.A.T., Houslay M.D., Davies S.A.
      Biochem. J. 388:333-342(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPDE11_DROME
    AccessioniPrimary (citable) accession number: Q9VJ79
    Secondary accession number(s): Q8MQW0, Q9VJ78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: January 15, 2008
    Last modified: June 24, 2015
    This is version 97 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.