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Protein

Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11

Gene

Pde11

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.1 Publication

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Kineticsi

  1. KM=18.5 µM for cAMP KM=6 µM for cGMP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei860Proton donorBy similarity1
    Metal bindingi864Divalent metal cation 1PROSITE-ProRule annotation1
    Metal bindingi900Divalent metal cation 1PROSITE-ProRule annotation1
    Metal bindingi901Divalent metal cation 1PROSITE-ProRule annotation1
    Metal bindingi901Divalent metal cation 2PROSITE-ProRule annotation1
    Metal bindingi1011Divalent metal cation 1PROSITE-ProRule annotation1
    Binding sitei1064cAMP or cGMPBy similarity1

    GO - Molecular functioni

    • 3',5'-cyclic-AMP phosphodiesterase activity Source: UniProtKB
    • 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • cAMP metabolic process Source: UniProtKB
    • cGMP metabolic process Source: UniProtKB
    • signal transduction Source: InterPro

    Keywordsi

    Molecular functionHydrolase
    LigandcAMP, cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiR-DME-418457 cGMP effects
    R-DME-418555 G alpha (s) signalling events

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11 (EC:3.1.4.35, EC:3.1.4.53)
    Alternative name(s):
    cAMP and cGMP phosphodiesterase 11
    Gene namesi
    Name:Pde11
    ORF Names:CG34341
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    Proteomesi
    • UP000000803 Componenti: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0085370 Pde11

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002470441 – 1451Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11Add BLAST1451

    Proteomic databases

    PaxDbiQ9VJ79
    PRIDEiQ9VJ79

    Expressioni

    Tissue specificityi

    In adults, it is enriched in Malpighian tubules.1 Publication

    Gene expression databases

    BgeeiFBgn0085370
    ExpressionAtlasiQ9VJ79 baseline and differential
    GenevisibleiQ9VJ79 DM

    Interactioni

    Protein-protein interaction databases

    BioGridi61102, 6 interactors
    STRINGi7227.FBpp0111456

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VJ79
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini419 – 572GAF 1Add BLAST154
    Domaini604 – 754GAF 2Add BLAST151
    Domaini783 – 1107PDEasePROSITE-ProRule annotationAdd BLAST325

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi1121 – 1124Poly-Gln4
    Compositional biasi1328 – 1436His-richAdd BLAST109

    Sequence similaritiesi

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiKOG3689 Eukaryota
    ENOG410XRI7 LUCA
    InParanoidiQ9VJ79
    KOiK13298
    OrthoDBiEOG091G04JU
    PhylomeDBiQ9VJ79

    Family and domain databases

    CDDicd00077 HDc, 1 hit
    Gene3Di1.10.1300.10, 1 hit
    3.30.450.40, 2 hits
    InterProiView protein in InterPro
    IPR003018 GAF
    IPR029016 GAF-like_dom_sf
    IPR003607 HD/PDEase_dom
    IPR023088 PDEase
    IPR002073 PDEase_catalytic_dom
    IPR036971 PDEase_catalytic_dom_sf
    IPR023174 PDEase_CS
    PfamiView protein in Pfam
    PF01590 GAF, 2 hits
    PF00233 PDEase_I, 1 hit
    PRINTSiPR00387 PDIESTERASE1
    SMARTiView protein in SMART
    SM00065 GAF, 2 hits
    SM00471 HDc, 1 hit
    PROSITEiView protein in PROSITE
    PS00126 PDEASE_I_1, 1 hit
    PS51845 PDEASE_I_2, 1 hit

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform B (identifier: Q9VJ79-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MGQAASMCRF RGCRYKNKNK SSKQQQQQQQ QQQQQQQQHQ QQQQQTPSHS
    60 70 80 90 100
    PQIQHHSEII PATTGLHLRS IEEPATTPLQ FQPTGRMNTE QGGTGYGGYG
    110 120 130 140 150
    SSEHSLLIAT RHAGVPLPLA QHQPLPAHYQ PLNHSGAAPP SSSNGSSSSG
    160 170 180 190 200
    GGVQTSATPQ QQQQYQVQQP YQYQYQHHYH HQANSPQHHR PYDPEHARME
    210 220 230 240 250
    AWLDENQEFV QDYFIRKATR QTVDAWLVSH ATSAGNDVVS STSPTHANGQ
    260 270 280 290 300
    TSSSRGGSGA TTPVRKISAH EFERGGLLKP IVNTIDGTPT FLSIGPPMDN
    310 320 330 340 350
    GSVGGSCSNL QNVGGVVAGQ YQYNHQQHHH NHAHLHHSQH SHYQAGGAVG
    360 370 380 390 400
    SSSLGSTGGA SGAGGAPSLG GSGGAGNGHQ YPYYHCHQRP QRLSRNELKQ
    410 420 430 440 450
    LDEKELIFEL VKDICNELEV RTLCHKILQN VSILLNADRG SLFLVQGRCN
    460 470 480 490 500
    GPDGLKKCLV SKLFDVCPRS TVEEMEQQDE VRVAWGTGIA GHVAESGEPV
    510 520 530 540 550
    NIPDAYQDER FNCEIDSLTG YRTKALLCMP IKDSSGDVIG VAQVINKMNG
    560 570 580 590 600
    ECFSEIDEKV FSSYLQFCGI GLRNAQLYEK SQLEIKRNQV LLDLARMIFE
    610 620 630 640 650
    EQSTIEHMVF RILTHMQSLI QCQRVQILLV HEADKGSFSR VFDFEANDLS
    660 670 680 690 700
    EEEATSRTSP YESRFPINIG ITGHVATTGE TVNVPNAYED DRFDASVDEN
    710 720 730 740 750
    SCFKHRSILC MAIKNSLGQI IGVIQLINKF NELDFTKNDE NFVEAFAIFC
    760 770 780 790 800
    GMGIHNTHMY EKAIVAMAKQ SVTLEVLSYH ASATMDEAHR LRRLRVPSAV
    810 820 830 840 850
    HFRLHDFKFD DIHFEDDDTL KACLRMFLDL DFVERFHIDY EVLCRWLLSV
    860 870 880 890 900
    KKNYRNVTYH NWRHAFNVAQ MMFAILTTTQ WWKIFGEIEC LALIIGCLCH
    910 920 930 940 950
    DLDHRGTNNS FQIKASSPLA QLYSTSTMEH HHFDQCLMIL NSPGNQILAN
    960 970 980 990 1000
    LSSDDYCRVI RVLEDAILST DLAVYFKKRG PFLESVSQPT SYWVAEEPRA
    1010 1020 1030 1040 1050
    LLRAMSMTVC DLSAITKPWE IEKRVADLVS SEFFEQGDME KQELNITPID
    1060 1070 1080 1090 1100
    IMNREKEDEL PMMQVNFIDS ICLPIYEAFA TLSDKLEPLV EGVRDNRGHW
    1110 1120 1130 1140 1150
    IDLADVVKTK TSQDQEPEEE QQQQNVISNG DCKAMSDDDV AASEAEVAVD
    1160 1170 1180 1190 1200
    SPSEKASVNG SNVANNSSNT NKKIAVASHP TSTQPSDDDN DVDADADDVD
    1210 1220 1230 1240 1250
    EQAAEENGHD AEVDEASCRS NSTCSSSTAS SCLSTPPPTG EDDSTPVSPL
    1260 1270 1280 1290 1300
    KTLQAKLVAA NLNALQRQTS NQAQTQKQRC KSCDHSRSGL QVRKTSSLRG
    1310 1320 1330 1340 1350
    AQELDLDSKT RNGTHAALCK STPVINNHSH HHNHSHSHNH NHHHHHHHHS
    1360 1370 1380 1390 1400
    HHNHSQHGIG IGSASIGGSG LISLTTPLLA MDSDRIPKIV GKIGNLDGLP
    1410 1420 1430 1440 1450
    FANGIGGPQN GHGLPFGSYQ HHHHHQHHHH LLARRHSETN SNGATAMAVE

    K
    Note: No experimental confirmation available.
    Length:1,451
    Mass (Da):160,930
    Last modified:January 15, 2008 - v4
    Checksum:iDFEC01058FB600A9
    GO
    Isoform C (identifier: Q9VJ79-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-44: Missing.
         45-64: QTPSHSPQIQHHSEIIPATT → MASSPNNAAPPVLWRARRKI

    Note: No experimental confirmation available.
    Show »
    Length:1,407
    Mass (Da):155,622
    Checksum:iDC01CA40E8325C1E
    GO
    Isoform D (identifier: Q9VJ79-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MKVTQSEENT...GLTFALLAGP

    Note: No experimental confirmation available.
    Show »
    Length:1,539
    Mass (Da):170,998
    Checksum:iE1EBAD64C5391214
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti38Q → QQQ in AAM52774 (PubMed:12537569).Curated1
    Sequence conflicti1192V → A in AAM52774 (PubMed:12537569).Curated1
    Sequence conflicti1196A → V in AAM52774 (PubMed:12537569).Curated1
    Sequence conflicti1232C → R in AAM52774 (PubMed:12537569).Curated1
    Sequence conflicti1342H → HNH in AAM52774 (PubMed:12537569).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0303281 – 44Missing in isoform C. CuratedAdd BLAST44
    Alternative sequenceiVSP_0199061M → MKVTQSEENTRNTSDRSKSV QTNRKFDNFNWLLSCGLLAA VKSTKLIPQLPRQQQPKKYN LRYAAELLKFDKIQFIKTEG LTFALLAGP in isoform D. 1 Publication1
    Alternative sequenceiVSP_03032945 – 64QTPSH…IPATT → MASSPNNAAPPVLWRARRKI in isoform C. CuratedAdd BLAST20

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE014134 Genomic DNA Translation: AAF53675.3
    AE014134 Genomic DNA Translation: AAF53676.2
    AY122262 mRNA Translation: AAM52774.1
    RefSeqiNP_001097177.1, NM_001103707.2 [Q9VJ79-3]
    NP_001286044.1, NM_001299115.1 [Q9VJ79-1]
    NP_001286045.1, NM_001299116.1 [Q9VJ79-3]
    NP_609885.2, NM_136041.2 [Q9VJ79-1]
    UniGeneiDm.13948

    Genome annotation databases

    EnsemblMetazoaiFBtr0112544; FBpp0111456; FBgn0085370 [Q9VJ79-1]
    FBtr0112545; FBpp0111457; FBgn0085370 [Q9VJ79-3]
    FBtr0343830; FBpp0310380; FBgn0085370 [Q9VJ79-1]
    FBtr0343831; FBpp0310381; FBgn0085370 [Q9VJ79-3]
    GeneIDi35107
    KEGGidme:Dmel_CG34341

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiPDE11_DROME
    AccessioniPrimary (citable) accession number: Q9VJ79
    Secondary accession number(s): Q8MQW0, Q9VJ78
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: January 15, 2008
    Last modified: May 23, 2018
    This is version 127 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

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