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Q9VJ79

- PDE11_DROME

UniProt

Q9VJ79 - PDE11_DROME

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Protein
Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11
Gene
Pde11, CG34341
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.1 Publication

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Kineticsi

  1. KM=18.5 µM for cAMP KM=6 µM for cGMP1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei860 – 8601Proton donor By similarity
Metal bindingi864 – 8641Divalent metal cation 1 By similarity
Metal bindingi900 – 9001Divalent metal cation 1 By similarity
Metal bindingi901 – 9011Divalent metal cation 1 By similarity
Metal bindingi901 – 9011Divalent metal cation 2 By similarity
Metal bindingi904 – 9041Divalent metal cation 2 By similarity
Metal bindingi929 – 9291Divalent metal cation 2 By similarity
Metal bindingi1011 – 10111Divalent metal cation 1 By similarity
Binding sitei1064 – 10641cAMP or cGMP By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: UniProtKB
  2. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. cAMP metabolic process Source: UniProtKB
  2. cGMP metabolic process Source: UniProtKB
  3. lateral inhibition Source: FlyBase
  4. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, cGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11 (EC:3.1.4.17, EC:3.1.4.35)
Alternative name(s):
cAMP and cGMP phosphodiesterase 11
Gene namesi
Name:Pde11
ORF Names:CG34341
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0085370. Pde11.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14511451Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11
PRO_0000247044Add
BLAST

Proteomic databases

PaxDbiQ9VJ79.

Expressioni

Tissue specificityi

In adults, it is enriched in Malpighian tubules.1 Publication

Gene expression databases

BgeeiQ9VJ79.

Interactioni

Protein-protein interaction databases

BioGridi61102. 6 interactions.
MINTiMINT-1027877.

Structurei

3D structure databases

ProteinModelPortaliQ9VJ79.
SMRiQ9VJ79. Positions 402-1137.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini419 – 572154GAF 1
Add
BLAST
Domaini604 – 754151GAF 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni836 – 1100265Catalytic By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1121 – 11244Poly-Gln
Compositional biasi1328 – 1436109His-rich
Add
BLAST

Sequence similaritiesi

Contains 2 GAF domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270709.
GeneTreeiENSGT00750000117253.
InParanoidiQ9VJ79.
KOiK13298.
OMAiCEIDSLT.
OrthoDBiEOG7RRF69.
PhylomeDBiQ9VJ79.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 2 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform B (identifier: Q9VJ79-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGQAASMCRF RGCRYKNKNK SSKQQQQQQQ QQQQQQQQHQ QQQQQTPSHS     50
PQIQHHSEII PATTGLHLRS IEEPATTPLQ FQPTGRMNTE QGGTGYGGYG 100
SSEHSLLIAT RHAGVPLPLA QHQPLPAHYQ PLNHSGAAPP SSSNGSSSSG 150
GGVQTSATPQ QQQQYQVQQP YQYQYQHHYH HQANSPQHHR PYDPEHARME 200
AWLDENQEFV QDYFIRKATR QTVDAWLVSH ATSAGNDVVS STSPTHANGQ 250
TSSSRGGSGA TTPVRKISAH EFERGGLLKP IVNTIDGTPT FLSIGPPMDN 300
GSVGGSCSNL QNVGGVVAGQ YQYNHQQHHH NHAHLHHSQH SHYQAGGAVG 350
SSSLGSTGGA SGAGGAPSLG GSGGAGNGHQ YPYYHCHQRP QRLSRNELKQ 400
LDEKELIFEL VKDICNELEV RTLCHKILQN VSILLNADRG SLFLVQGRCN 450
GPDGLKKCLV SKLFDVCPRS TVEEMEQQDE VRVAWGTGIA GHVAESGEPV 500
NIPDAYQDER FNCEIDSLTG YRTKALLCMP IKDSSGDVIG VAQVINKMNG 550
ECFSEIDEKV FSSYLQFCGI GLRNAQLYEK SQLEIKRNQV LLDLARMIFE 600
EQSTIEHMVF RILTHMQSLI QCQRVQILLV HEADKGSFSR VFDFEANDLS 650
EEEATSRTSP YESRFPINIG ITGHVATTGE TVNVPNAYED DRFDASVDEN 700
SCFKHRSILC MAIKNSLGQI IGVIQLINKF NELDFTKNDE NFVEAFAIFC 750
GMGIHNTHMY EKAIVAMAKQ SVTLEVLSYH ASATMDEAHR LRRLRVPSAV 800
HFRLHDFKFD DIHFEDDDTL KACLRMFLDL DFVERFHIDY EVLCRWLLSV 850
KKNYRNVTYH NWRHAFNVAQ MMFAILTTTQ WWKIFGEIEC LALIIGCLCH 900
DLDHRGTNNS FQIKASSPLA QLYSTSTMEH HHFDQCLMIL NSPGNQILAN 950
LSSDDYCRVI RVLEDAILST DLAVYFKKRG PFLESVSQPT SYWVAEEPRA 1000
LLRAMSMTVC DLSAITKPWE IEKRVADLVS SEFFEQGDME KQELNITPID 1050
IMNREKEDEL PMMQVNFIDS ICLPIYEAFA TLSDKLEPLV EGVRDNRGHW 1100
IDLADVVKTK TSQDQEPEEE QQQQNVISNG DCKAMSDDDV AASEAEVAVD 1150
SPSEKASVNG SNVANNSSNT NKKIAVASHP TSTQPSDDDN DVDADADDVD 1200
EQAAEENGHD AEVDEASCRS NSTCSSSTAS SCLSTPPPTG EDDSTPVSPL 1250
KTLQAKLVAA NLNALQRQTS NQAQTQKQRC KSCDHSRSGL QVRKTSSLRG 1300
AQELDLDSKT RNGTHAALCK STPVINNHSH HHNHSHSHNH NHHHHHHHHS 1350
HHNHSQHGIG IGSASIGGSG LISLTTPLLA MDSDRIPKIV GKIGNLDGLP 1400
FANGIGGPQN GHGLPFGSYQ HHHHHQHHHH LLARRHSETN SNGATAMAVE 1450
K 1451

Note: No experimental confirmation available.

Length:1,451
Mass (Da):160,930
Last modified:January 15, 2008 - v4
Checksum:iDFEC01058FB600A9
GO
Isoform C (identifier: Q9VJ79-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     45-64: QTPSHSPQIQHHSEIIPATT → MASSPNNAAPPVLWRARRKI

Note: No experimental confirmation available.

Show »
Length:1,407
Mass (Da):155,622
Checksum:iDC01CA40E8325C1E
GO
Isoform D (identifier: Q9VJ79-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKVTQSEENT...GLTFALLAGP

Note: No experimental confirmation available.

Show »
Length:1,539
Mass (Da):170,998
Checksum:iE1EBAD64C5391214
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4444Missing in isoform C.
VSP_030328Add
BLAST
Alternative sequencei1 – 11M → MKVTQSEENTRNTSDRSKSV QTNRKFDNFNWLLSCGLLAA VKSTKLIPQLPRQQQPKKYN LRYAAELLKFDKIQFIKTEG LTFALLAGP in isoform D.
VSP_019906
Alternative sequencei45 – 6420QTPSH…IPATT → MASSPNNAAPPVLWRARRKI in isoform C.
VSP_030329Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381Q → QQQ in AAM52774. 1 Publication
Sequence conflicti1192 – 11921V → A in AAM52774. 1 Publication
Sequence conflicti1196 – 11961A → V in AAM52774. 1 Publication
Sequence conflicti1232 – 12321C → R in AAM52774. 1 Publication
Sequence conflicti1342 – 13421H → HNH in AAM52774. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014134 Genomic DNA. Translation: AAF53675.3.
AE014134 Genomic DNA. Translation: AAF53676.2.
AY122262 mRNA. Translation: AAM52774.1.
RefSeqiNP_001097177.1. NM_001103707.1. [Q9VJ79-3]
NP_609885.2. NM_136041.2. [Q9VJ79-1]
UniGeneiDm.13948.

Genome annotation databases

EnsemblMetazoaiFBtr0112544; FBpp0111456; FBgn0085370. [Q9VJ79-1]
GeneIDi35107.
KEGGidme:Dmel_CG34341.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014134 Genomic DNA. Translation: AAF53675.3 .
AE014134 Genomic DNA. Translation: AAF53676.2 .
AY122262 mRNA. Translation: AAM52774.1 .
RefSeqi NP_001097177.1. NM_001103707.1. [Q9VJ79-3 ]
NP_609885.2. NM_136041.2. [Q9VJ79-1 ]
UniGenei Dm.13948.

3D structure databases

ProteinModelPortali Q9VJ79.
SMRi Q9VJ79. Positions 402-1137.
ModBasei Search...

Protein-protein interaction databases

BioGridi 61102. 6 interactions.
MINTi MINT-1027877.

Proteomic databases

PaxDbi Q9VJ79.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0112544 ; FBpp0111456 ; FBgn0085370 . [Q9VJ79-1 ]
GeneIDi 35107.
KEGGi dme:Dmel_CG34341.

Organism-specific databases

CTDi 35107.
FlyBasei FBgn0085370. Pde11.

Phylogenomic databases

eggNOGi NOG270709.
GeneTreei ENSGT00750000117253.
InParanoidi Q9VJ79.
KOi K13298.
OMAi CEIDSLT.
OrthoDBi EOG7RRF69.
PhylomeDBi Q9VJ79.

Miscellaneous databases

GenomeRNAii 35107.
NextBioi 791899.

Gene expression databases

Bgeei Q9VJ79.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 2 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
    Strain: Berkeley.
    Tissue: Embryo.
  4. "Cyclic nucleotide phosphodiesterases in Drosophila melanogaster."
    Day J.P., Dow J.A.T., Houslay M.D., Davies S.A.
    Biochem. J. 388:333-342(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPDE11_DROME
AccessioniPrimary (citable) accession number: Q9VJ79
Secondary accession number(s): Q8MQW0, Q9VJ78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 15, 2008
Last modified: July 9, 2014
This is version 89 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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