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Q9VJ79 (PDE11_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11

EC=3.1.4.17
EC=3.1.4.35
Alternative name(s):
cAMP and cGMP phosphodiesterase 11
Gene names
Name:Pde11
ORF Names:CG34341
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1451 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively. Ref.4

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Tissue specificity

In adults, it is enriched in Malpighian tubules. Ref.4

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 2 GAF domains.

Biophysicochemical properties

Kinetic parameters:

KM=18.5 µM for cAMP KM=6 µM for cGMP Ref.4

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: Q9VJ79-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform C (identifier: Q9VJ79-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     45-64: QTPSHSPQIQHHSEIIPATT → MASSPNNAAPPVLWRARRKI
Note: No experimental confirmation available.
Isoform D (identifier: Q9VJ79-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKVTQSEENT...GLTFALLAGP
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14511451Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11
PRO_0000247044

Regions

Domain419 – 572154GAF 1
Domain604 – 754151GAF 2
Region836 – 1100265Catalytic By similarity
Compositional bias1121 – 11244Poly-Gln
Compositional bias1328 – 1436109His-rich

Sites

Active site8601Proton donor By similarity
Metal binding8641Divalent metal cation 1 By similarity
Metal binding9001Divalent metal cation 1 By similarity
Metal binding9011Divalent metal cation 1 By similarity
Metal binding9011Divalent metal cation 2 By similarity
Metal binding9041Divalent metal cation 2 By similarity
Metal binding9291Divalent metal cation 2 By similarity
Metal binding10111Divalent metal cation 1 By similarity
Binding site10641cAMP or cGMP By similarity

Natural variations

Alternative sequence1 – 4444Missing in isoform C.
VSP_030328
Alternative sequence11M → MKVTQSEENTRNTSDRSKSV QTNRKFDNFNWLLSCGLLAA VKSTKLIPQLPRQQQPKKYN LRYAAELLKFDKIQFIKTEG LTFALLAGP in isoform D.
VSP_019906
Alternative sequence45 – 6420QTPSH…IPATT → MASSPNNAAPPVLWRARRKI in isoform C.
VSP_030329

Experimental info

Sequence conflict381Q → QQQ in AAM52774. Ref.3
Sequence conflict11921V → A in AAM52774. Ref.3
Sequence conflict11961A → V in AAM52774. Ref.3
Sequence conflict12321C → R in AAM52774. Ref.3
Sequence conflict13421H → HNH in AAM52774. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform B [UniParc].

Last modified January 15, 2008. Version 4.
Checksum: DFEC01058FB600A9

FASTA1,451160,930
        10         20         30         40         50         60 
MGQAASMCRF RGCRYKNKNK SSKQQQQQQQ QQQQQQQQHQ QQQQQTPSHS PQIQHHSEII 

        70         80         90        100        110        120 
PATTGLHLRS IEEPATTPLQ FQPTGRMNTE QGGTGYGGYG SSEHSLLIAT RHAGVPLPLA 

       130        140        150        160        170        180 
QHQPLPAHYQ PLNHSGAAPP SSSNGSSSSG GGVQTSATPQ QQQQYQVQQP YQYQYQHHYH 

       190        200        210        220        230        240 
HQANSPQHHR PYDPEHARME AWLDENQEFV QDYFIRKATR QTVDAWLVSH ATSAGNDVVS 

       250        260        270        280        290        300 
STSPTHANGQ TSSSRGGSGA TTPVRKISAH EFERGGLLKP IVNTIDGTPT FLSIGPPMDN 

       310        320        330        340        350        360 
GSVGGSCSNL QNVGGVVAGQ YQYNHQQHHH NHAHLHHSQH SHYQAGGAVG SSSLGSTGGA 

       370        380        390        400        410        420 
SGAGGAPSLG GSGGAGNGHQ YPYYHCHQRP QRLSRNELKQ LDEKELIFEL VKDICNELEV 

       430        440        450        460        470        480 
RTLCHKILQN VSILLNADRG SLFLVQGRCN GPDGLKKCLV SKLFDVCPRS TVEEMEQQDE 

       490        500        510        520        530        540 
VRVAWGTGIA GHVAESGEPV NIPDAYQDER FNCEIDSLTG YRTKALLCMP IKDSSGDVIG 

       550        560        570        580        590        600 
VAQVINKMNG ECFSEIDEKV FSSYLQFCGI GLRNAQLYEK SQLEIKRNQV LLDLARMIFE 

       610        620        630        640        650        660 
EQSTIEHMVF RILTHMQSLI QCQRVQILLV HEADKGSFSR VFDFEANDLS EEEATSRTSP 

       670        680        690        700        710        720 
YESRFPINIG ITGHVATTGE TVNVPNAYED DRFDASVDEN SCFKHRSILC MAIKNSLGQI 

       730        740        750        760        770        780 
IGVIQLINKF NELDFTKNDE NFVEAFAIFC GMGIHNTHMY EKAIVAMAKQ SVTLEVLSYH 

       790        800        810        820        830        840 
ASATMDEAHR LRRLRVPSAV HFRLHDFKFD DIHFEDDDTL KACLRMFLDL DFVERFHIDY 

       850        860        870        880        890        900 
EVLCRWLLSV KKNYRNVTYH NWRHAFNVAQ MMFAILTTTQ WWKIFGEIEC LALIIGCLCH 

       910        920        930        940        950        960 
DLDHRGTNNS FQIKASSPLA QLYSTSTMEH HHFDQCLMIL NSPGNQILAN LSSDDYCRVI 

       970        980        990       1000       1010       1020 
RVLEDAILST DLAVYFKKRG PFLESVSQPT SYWVAEEPRA LLRAMSMTVC DLSAITKPWE 

      1030       1040       1050       1060       1070       1080 
IEKRVADLVS SEFFEQGDME KQELNITPID IMNREKEDEL PMMQVNFIDS ICLPIYEAFA 

      1090       1100       1110       1120       1130       1140 
TLSDKLEPLV EGVRDNRGHW IDLADVVKTK TSQDQEPEEE QQQQNVISNG DCKAMSDDDV 

      1150       1160       1170       1180       1190       1200 
AASEAEVAVD SPSEKASVNG SNVANNSSNT NKKIAVASHP TSTQPSDDDN DVDADADDVD 

      1210       1220       1230       1240       1250       1260 
EQAAEENGHD AEVDEASCRS NSTCSSSTAS SCLSTPPPTG EDDSTPVSPL KTLQAKLVAA 

      1270       1280       1290       1300       1310       1320 
NLNALQRQTS NQAQTQKQRC KSCDHSRSGL QVRKTSSLRG AQELDLDSKT RNGTHAALCK 

      1330       1340       1350       1360       1370       1380 
STPVINNHSH HHNHSHSHNH NHHHHHHHHS HHNHSQHGIG IGSASIGGSG LISLTTPLLA 

      1390       1400       1410       1420       1430       1440 
MDSDRIPKIV GKIGNLDGLP FANGIGGPQN GHGLPFGSYQ HHHHHQHHHH LLARRHSETN 

      1450 
SNGATAMAVE K 

« Hide

Isoform C [UniParc].

Checksum: DC01CA40E8325C1E
Show »

FASTA1,407155,622
Isoform D [UniParc].

Checksum: E1EBAD64C5391214
Show »

FASTA1,539170,998

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[3]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
Strain: Berkeley.
Tissue: Embryo.
[4]"Cyclic nucleotide phosphodiesterases in Drosophila melanogaster."
Day J.P., Dow J.A.T., Houslay M.D., Davies S.A.
Biochem. J. 388:333-342(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014134 Genomic DNA. Translation: AAF53675.3.
AE014134 Genomic DNA. Translation: AAF53676.2.
AY122262 mRNA. Translation: AAM52774.1.
RefSeqNP_001097177.1. NM_001103707.1.
NP_609885.2. NM_136041.2.
UniGeneDm.13948.

3D structure databases

ProteinModelPortalQ9VJ79.
SMRQ9VJ79. Positions 402-1137.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid61102. 6 interactions.
MINTMINT-1027877.

Proteomic databases

PaxDbQ9VJ79.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0112544; FBpp0111456; FBgn0085370. [Q9VJ79-1]
GeneID35107.
KEGGdme:Dmel_CG34341.

Organism-specific databases

CTD35107.
FlyBaseFBgn0085370. Pde11.

Phylogenomic databases

eggNOGNOG270709.
GeneTreeENSGT00750000117253.
InParanoidQ9VJ79.
KOK13298.
OMACEIDSLT.
OrthoDBEOG7RRF69.
PhylomeDBQ9VJ79.

Gene expression databases

BgeeQ9VJ79.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
InterProIPR003018. GAF.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi35107.
NextBio791899.

Entry information

Entry namePDE11_DROME
AccessionPrimary (citable) accession number: Q9VJ79
Secondary accession number(s): Q8MQW0, Q9VJ78
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 86 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase