Reviewed, UniProtKB/Swiss-Prot Q9VJ62 (NAF1_DROME)

Names and origin

Protein names

Recommended name:
    H/ACA ribonucleoprotein complex non-core subunit NAF1

Gene names

ORF Names:

CG10341

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by GAR1/CG4038 to yield mature H/ACA snoRNPs complex By similarity.
Subunit structure	During assembly of the complex, component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs) By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the NAF1 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell small nucleolar ribonucleoprotein complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	RNA binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gbp	Q9V3B2	1	EBI-154062,EBI-115372

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	H/ACA ribonucleoprotein complex non-core subunit NAF1		PRO_0000315640
Regions
	Compositional bias	197 – 216	20	Ser-rich
	Compositional bias	516 – 563	48	Pro-rich
Amino acid modifications
	Modified residue	250	1	Phosphothreonine Ref.4
	Modified residue	254	1	Phosphoserine Ref.4
	Modified residue	403	1	Phosphoserine Ref.4
	Modified residue	427	1	Phosphothreonine Ref.4
	Modified residue	429	1	Phosphoserine Ref.4
	Modified residue	432	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9VJ62-1 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 283FB6B26F415044	FASTA	564	62,270
	10 20 30 40 50 60 MESEQPAAVK ASAPIEEPQS TETAVELGKH STLKADLSID KLETATTEAT NAVVEPVEME 70 80 90 100 110 120 QQVENELVNE STQAMTSTTT QEKVVGSVNN LVEEQQCVEM EFETSSDLVV QGSPSEESKK 130 140 150 160 170 180 VASDASATVT APQVQPAPHV DSSPQASGLS LLAAYSSDDS DDEKVTPVQN GDNDVIEVPV 190 200 210 220 230 240 TDPASSTTAY RPVVAVSSDD ESSKSSSSSS DSDSDSEGEY LTVLRKKIDK RINTVDCDED 250 260 270 280 290 300 DEDFDEDGAT GDRSRRRQPP KVRGEMLLDD LPPIHQLEIT VPEDECVELG KVQSIVDQLV 310 320 330 340 350 360 LVSVLPNSML FDLDTVLFLE KGRKVLGEVF DVLGQVSDPL YCVRFNSNKQ ILDRGIKIGD 370 380 390 400 410 420 VVYCAPKTEH TQFVILSKLM QVRGSDASWE HDVEPPARYV DHSDDEEERE ARAEQRKRRQ 430 440 450 460 470 480 RDRTNSTDSV DTVTSVATTA TKASSVAPPP RQRGRRGQRE SFRQSQRPSI NQHNQNQPQD 490 500 510 520 530 540 EQYNFHPSYN PGSWHSNYYQ NYHQAAANFN MAQQHPGMPF PVPNYGYGMP YAMPPMYPHM 550 560 YPPPPPFAPP PPNNQSHQGQ PPPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[4]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-250; SER-254; SER-403; THR-427; SER-429 AND THR-432, MASS SPECTROMETRY. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014134 Genomic DNA. Translation: AAF53693.2. AY051589 mRNA. Translation: AAK93013.1.
RefSeq	NP_609898.1.
UniGene	Dm.23213
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VJ62. 4 interactions.
Genome annotation databases
Ensembl	FBgn0032701. Drosophila melanogaster. [Contig view]
GeneID	35125.
NMPDR	fig|7227.3.peg.2858.
Organism-specific databases
FlyBase	FBgn0032701. CG10341.
Phylogenomic databases
HOGENOM	Q9VJ62.
OMA	Q9VJ62. SEGEYLT.
Family and domain databases
InterPro	IPR008696. NAF1. [Graphical view]
Pfam	PF05492. NAF1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	791988.

Entry information

Entry name

NAF1_DROME

Accession

Primary (citable) accession number: Q9VJ62
Secondary accession number(s): Q961H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project